Nacalai Tesque, Inc. Reagents Immunologocal Products


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Epitope Tag Antibody

Nacalai Tesque carries an impressive variety of antibodies. Apart from the wide range of secondary antibodies, recently tag antibodies are developed in increased numbers. Presently available tagged antibody types are: GFP, c-Myc, His, V5, GST. Most of these types are available in POD coupled form as well.
Uniquely, GFP (Rat IgG2a) is suitable for immunohistochemical detection!


Anti-GFP (Rat IgG2a), Monoclonal(GF090R)

Feature
  • Immunohistochemical application
  • Rat monoclonal antibody
Clone : GF090R
Isotype : IgG2a(Rat)
Product form : Liquid
Immunogen : Liquid
Application:
Immunohistochemstry 1:1000 - 1:2000
Western blotting 1:1000 - 1:2000
ELISA 1:2000 - 1:20000
Immunocytochemistry
Sample : Mouse brain (nerve cell)
Primary antibody : Anti-GFP(Rat IgG2a), Monoclonal (1:1000) RT, O/N
Secondary antibody : Anti-Rat IgG-Cy3 (1:300) RT, 1hr
Blocking : 5% normal goat serum / 0.2% TritonX-100 in PBS
Fixing method : 4% Paraformaldehyde
Data courtesy of Dr.Yoshihara Y., RIKEN Brain Science Institute


Reference
  1. Nakashiba T et al. Science 319(5867), 1260-1264 (2008)
  2. Batista-Brito R et al. The Journal of Neuroscience 28(15), 3966-3975 (2008)
  3. Esumi S et al. Neuroscience Research 60(4), 439-451 (2008)
  4. Fogarty M et al. The Journal of Neuroscience 27(41), 10935-10946 (2007)
  5. Sasamura T et al. Development 134, 1347-1356 (2007)
  6. Sato Y et al. The Journal of Neuroscience 27(7), 1606-1615 (2007)
  7. Yamada M et al. Stem Cells 25(3), 562-570 (2007)
  8. Togashi H et al. The Journal of Cell Biology 174, 141-151 (2006)
  9. Ogata M. et al. Molecular and Cell Biology 26(24), 9220-9231 (2006)
  10. Sato Y et al. The Journal of Neuroscience 25(20), 4889-4897 (2005)

Anti-cMyc(mouse IgG1-k), Monoclonal
Clone : MC045
Isotype : IgG1-κ(mouse)
Product form : Liquid
Immunogen : c-Myc synthetic peptide [EQKLISEEDL] conjugated with KLH
Application:
Western blotting 1:1000 - 1:2000
Immunoprecipitation 1:400 - 1:1000
Immunocytochemistry 1:400 - 1:1000
ELISA 1:2000 - 1:20000
Immunocytochemistry
Sample : SH-SY5Y cell in which c-Myc-tagged TCP1β is expressed
Primary antibody : Anti-c-Myc (Mouse IgG1-κ) (2.5mg/ml) Anti-Protein X

Secondary antibody :
Alexa Fluor 546-conjugated antibody (1:400)
Alexa Fluor 488-conjugated antibody (1:400)

Data courtesy of RIKEN Brain Science Institute
 
Sample : HeLa cell in which c-Myc-tagged(N-terminal) hnRNP A1 is expressed
Primary antibody : Anti-c-Myc (Mouse IgG1-κ) (1:1000)
Secondary antibody : FITC-conjugated goat affinity purified f(ab')2 fragment to mouse IgG (whole molecule) (1:200)
Fixation method : 2% Formaldehyde in PBS (RT, 30min)
Permeabilization : Acetone (-20°C, 3min)
Blocking : 3% BSA in PBS (RT)
Data courtesy of Dr. Kataoka N., Department of Genetics and Molecular Biology,
Laboratry of Biochemistry, Institute for Virus Research, Kyoto University.

Anti-GFP (Mouse IgG1-k), Monoclonal
Clone : GF200
Isotype : IgG1-κ(Mouse)
Product form : Liquid
Immunogen : His-GFP (full length) fusion protein
Application:
Western blotting 1:1000 - 1:2000
ELISA 1:2000 - 1:20000
Western blotting
Sample :
lane 1) HeLa:GFP-H2A-Bbd
lane 2) HeLa:GFP-H2B
lane 3) HeLa
Data courtesy of Dr. Kimura H., Horizontal Medical Research Organization,
Kyoto University Faculty of Medicine
Western blotting
Protein extract from seedlings of Arabidopsis (10mg)
Lane1 : Non-transformants (Control)
Lane2 : Transformants

Primary antibody:
Anti-GFP(mouse IgG-κ) (1:5000)
Secondary antibody:
Anti-mouse IgG-HRP (1:2000)
Data courtesy of Dr. Shimada T., Department of Botany, Graduate School of Science, Kyoto University

Clone : GS019
Isotype : IgG2a-κ(mouse)
Product form : Liquid
Immunogen : Glutathione - S - Transferase(GST)
Application:
Western blotting 1:1000 - 1:2000
Immunoprecipitation 1:400 - 1:1000
ELISA 1:2000 - 1:20000
Western blotting
 
Gel: 12.5% SDS-PAGE
Blocking: Blocking One (Nacalai Tesque) / 1 hr at RT
Primary antibody: anti-GST (Mouse IgG2a-κ),(GS019) (1:2000 with Blocking One) / 1 hr at RT
Wash: TBS-T (5 min x 3)
Secondary antibody: anti-mouse Ig-HRP(1:1000 with TBS-T) / 30 min at RT
Wash : TBS-T (5 min x 3)
Detection: Luminol Reagent (Santa Cruz)
Sample :
lane 1: Lysate from E.coli expressioning GST protein
lane 2: Glutathione Sepharose flow-through fraction
lane 3: Glutathione Sepharose elution fraction
Data courtesy of Dr. Shimizu Y., The Institute of Medical Science,
The University of Tokyo

Anti-V5(Mouse IgG1-k), Monoclonal(V5005)
Clone
Isotype
Product form
Immunogen 		: V5005
: IgG1-κ(mouse)
: Liquid
: V5 synthetic peptide [GKPIPNPLLGLDST] conjugated with KLH
Application Western blotting 1:1000 - 1:2000
Immunoprecipitation 1:400 - 1:1000
ELISA 1:2000 - 1:20000
Western blotting, Immunoprecipitation
(A) Western blotting
Sample:
Lysate from 293T cell in which V5-tagged (C-terminal) pCDNA dest40-VE-cadherin is expressed
Antibody: Anti-V5 (Mouse IgG1-κ) (1:5000)
(B) Immunoprecipitation
Sample: Lysate (A)
Antibody: Anti-V5 (Mouse IgG1-κ) (1µl)
Western blotting
Antibody: Anti-V5 (Mouse IgG1-κ) (1:2000)

Anti-6xHis(Mouse IgG1-k), Monoclonal(HI192)
Clone
Isotype
Product form
Immunogen 		: HI192
: IgG1-κ(mouse)
: Liquid
: 6xHis synthetic peptide [HHHHHH] conjugated with KLH
Application Western blotting 1:1000 - 1:2000
ELISA 1:2000 - 1:20000
Filter: FluoroTrans[PALL]
Blocking: 5% non-fat dry milk/PBS (30 min)
Primary antibody: anti-6xHis (Mouse IgG1-κ),Monoclonal (HI192)
Wash: 0.25% Tween-20/TBS (10 min x 3)
Secondary antibody: anti-mouse IgG-HRP(1:1000) / 2% BSA / 0.25% Tween-20/TBS (30 min)
Wash: 0.25% Tween-20/TBS (10 min x 5)
Detection: Luminol Reagent (Santa Cruz)
Sample: lysate from Sf 9 cells in which His-tagged Drosophila PTEN is expressed

Data courtesy of Dr. Maehama A., The Tokyo Metropolitan Institute
of Medical Science (RINSHOKEN)

<< Ordering Information Tag Antibodies >>
Product name Grade Storage Code No.
Quantity
Anti-GFP (Rat IgG2a), Monoclonal (GF090R)
 BC R 04404-26 50µg
04404-84 200µg
Anti-c-Myc (Mouse IgG1-κ), Monoclonal (MC045) BC R 04362-76 50µg
04362-34 200µg
Anti-GFP (Mouse IgG1-κ), Monoclonal (GF200) BC R 04363-66 50µg
04363-24 200µg
Anti-GST (Mouse IgG2a-κ), Monoclonal (GS019) BC R 04435-84 50µg
04435-26 200µg
Anti-V5 (Mouse IgG1-κ), Monoclonal (V5005) BC R 04434-94 50µg
04434-36 200µg
Anti-6xHis (Mouse IgG1-κ), Monoclonal (HI192) BC R 04428-26 50µg
04428-84 200µg

< POD Conjugated Tag Antibodies >
Product name Grade Storage Code No.
Quantity
Anti-c-Myc (Mouse IgG1-κ),Mono (MC045),POD Conjugated BC R 04554-24 50µg
Anti-GFP (Mouse IgG1-κ), Mono (GF200),POD Conjugated BC R 05178-34 50µg
Anti-GST (Mouse IgG2a-κ), Mono (GS019),POD Conjugated BC R 04559-74 50µg
Anti-V5 (Mouse IgG1-κ), Mono (V5005),POD Conjugated BC R 04578-24 50µg
Anti-6xHis (Mouse IgG1-κ), Mono (HI192),POD Conjugated BC R 04546-34 50µg

< Agarose Conjugated Tag Antibodies>
Product name Grade Storage Code No.
Quantity
Anti-c-Myc (Mouse IgG1-κ),Mono (MC045), Agarose Conjugated BC R 04145-55 500µg


Printed version(PDF 198KB)

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Quantity
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Blocking One SP R 03953-95 500 ml
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For research use only, not intended for diagnostic or drug use.
(Storage)
A = Cool and dark, RT = Room temperature, F= Freezer, R= Refrigerator



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