NACALAI TESQUE, INC.

Ultra-pure silica gel of more than 99.99% purity is used for the COSMOSIL SL-II packed column series. This column provides improved separation and reproducibility for compounds with carbonyl or phenol hydroxyl groups, which are often problematic to separate using conventional silica gel columns.

Material characteristics

Packing material	SL-II
Silica gel	high purity porous spherical silica
Average particle size	3, 5, 15 μm
Average pore size	approx. 120 A
Specific surface area	approx. 300 m2/g
Stationary phase	High purity Silica Gel (>99.99%) with specially treatment Suitable for preparative separation

Analysis of acids and amide without ionic additives

Condition
Column size	4.6 mm I.D. × 250 mm	Detection	UV 254 nm, 0.5AUFS
Mobile phase	methanol : hexane = 10 : 90	Sample	Benzoic Acid  4.0 μg Salicylic Acid  6.0 μg Salicylamide  4.0 μg
Flow rate	1 ml/min
Temperature	30°C

Scaling up from analytical to preparative separation

Step 1	Step 2	Step 3
Mobile phase is optimized by using 4.6 mm I.D. × 250 mm column	Maximum load capacity is optimized by using 4.6 mm I.D. × 250 mm column	Preparative separation by using 20 mm I.D. × 250 mm column
As mobile phase, highly soluble solvent for sample is suitable.	Maximum injection volume 56 μl (28 mg)	Maximum injection volume 1,050 μl (525 mg) (19 times higher than 4.6 mm I.D.)

Condition
Mobile phase	ethyl acetate : hexane = 10 : 90	Sample	1.1-Naphthol
Flow rate	4.6 mm I.D. : 1 ml/min 20 mm I.D. : 18.9 ml/min
Temperature	room temperature		2.2-Naphthol
Detection	UV 254 nm

Ordering information

Analytical and preparative column (Particle size : 5 μm)

COSMOSIL 5SL-II

Product name	Size	Product No.
COSMOSIL 5SL-II Guard Column
	4.6 mm l.D.×10 mm	37997-01
	10 mm l.D.×20 mm	37998-91
	20 mm l.D.×20 mm	05874-91
	20 mm l.D.×50 mm	05875-81
	28 mm l.D.×50 mm	34359-51
COSMOSIL 5SL-II Packed Column
	4.6 mm l.D.×50 mm	37999-81
	4.6 mm l.D.×100 mm	38000-01
	4.6 mm l.D.×150 mm	38001-91
	4.6 mm l.D.×250 mm	38002-81
	6.0 mm l.D.×150 mm	38003-71
	6.0 mm l.D.×250 mm	38004-61
	10 mm l.D.×250 mm	38005-51
	20 mm l.D.×250 mm	38006-41
	28 mm l.D.×250 mm	34358-61

Preparative column (Particle size : 15 μm)

COSMOSIL 15SL-II

Product name	Size	Product No.
COSMOSIL 15SL-II Guard Column
	28 mm l.D.×50 mm	05892-51
	50 mm l.D.×50 mm	05894-31
COSMOSIL 15SL-II Packed Column
	28 mm l.D.×250 mm	05893-41
	50 mm l.D.×250 mm	05895-21
	50 mm l.D.×500 mm	05896-11

Fast LC column (Particle size : 3 μm)

COSMOSIL 3SL-II

Product name	Size	Product No.
COSMOSIL 3SL-II Packed Column
	4.6 mm l.D.×10 mm	38059-61
	4.6 mm l.D.×50 mm	38060-21
	4.6 mm l.D.×100 mm	38061-11