NACALAI TESQUE, INC.

LEXSY in vitro Translation Kit  

The LEXSY in vitro translation system is a NEW rapid, convenient, flexible and cost efficient tool for cell-free production of recombinant proteins for biochemical, biophysical and structural analysis.
The LEXSY cell extract for in vitro translation contains functional ribosomes and all essential components of the eukaryotic translation and folding machinery. For target mRNA generation the heterologous T7 RNA polymerase was added to the extracts. To ensure a low background the translation of endogenous host mRNAs is efficiently blocked by an antisense oligonucleotide making use of the unique gene organization of Leishmania.

Vector map

Map of pLEXSY_invitro-1 (Cat.-No. EGE-261) expression vector with cloning sites for target gene in frame fusions. 3’ utr is derived from 1.4k-IR camCB of L. tarentolae. MCS1 and MCS2 are multicloning sites for replacement of vector EGFP control gene or N’ or C’ fusions resp. SITS is species independent translation sequence (Mureev et al., 2009).

Kit components

Ordering information