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Low cytotoxicity & superior reagent for primary cell transfection
Glycofect^TM Transfection Reagent

Glycofect is a cationic carbohydrate-containing synthetic polymer based on the　poly-glycoamidoamine (PGAA) polymer library developed by Dr. Theresa M. Reineke et al. These polymers contain linkages that hydrolyse upon endocytosis, thereby increasing plasmid delivery and reducing toxicity. Glycofect is a superior reagent for primary cell transfection as compared to leading lipid and poly-cationic based products.

Features

Effective delivery with low cytotoxicity
Effective cellular uptake through nanoparticle delivery
Protects DNA from nuclease degradation
Biodegrades after endocytosis
Simple & robust transfection procedure
Exceptional results in primary cells

Documented Results-1

The following micrographs compare Glycofect to leading lipid-based reagents when used to transfect primary dermal fibroblasts and primary aortic smooth muscle cells. In each series, the DIC images (top row) show the density of the individual cells while the fluorescence iimages (bottom row) show those cells that have been transfected with a plasmid expressing green fluorescent protein. All samples were prepared per manufacturers' instructions.
■ Primary Dermal Fibroblasts

■ Primary Human Aortic Smooth Muscle

Documented Results-2

■ Primary Dermal Fibroblasts

Primary Dermal Fibroblasts (left) transfected with green fluorescent protein using a leading lipid-　based reagent (center) and Glycofect Transfection Reagent (right).

Technical References

T.M. Reineke "PGAAs: " Cationic Glycopolymers for DNA Delivery" J. Polym. Sci. A: Polym. Chem. 2006, 44, 6895-6908.

Y. Liu, T.M. Reineke, "Poly(glycoamidoamine)s for Gene Delivery. Stability of Polyplexes and Efficacy with Cardiomyoblast Cells" Bioconjugate Chem. 2006, 17, 101-108.

Y. Liu, T.M. Reineke, "Degradation of Poly(glycoamidoamine) DNA Delivery Vehicles: Polyamide Hydrolysis at Physiological Conditions Promotes DNA Release." Biomacromolecules, 2010, 11, 316.

Y. Liu, T.M. Reineke, "Hydroxyl Stereochemistry and Amine Number wihin Poly(glycoamidoamine)s Affect Intracellular DNA Delivery." J. Am. Chem. Soc., 2005, 127, 3004