Products

COSMOCORE 2.6C18

Features

Coreja

  • 2.6 µm Core Shell Particle
  • Increased loading capacity
  • Excellent pH stability (1.5-10)

>> Information on COSMOCORE 2.6Cholester

>> Information on COSMOCORE 2.6PBr

Product Information

About Core-Shell Particles

About Core-Shell Particles

Column Specifications

Column Specifications

Excellent pH Stability

Under accelerated pH 10.4, 40°C stability test, COSMOCORE C18 column shows superior stability compared with other core shell C18 phases.

pH stability

pH stability

Test condition
Column size 2.1 mm I.D. x 100 mm Temperature 40 °C
Mobile phase: 0.35% ammonium hydroxide/ acetonitrile = 90/10 (pH 10.4) Sample caffeine 0.05 mg/ml
Flow rate 0.4 ml/min. Inj.Vol. 1 µl

Reduced Back Pressure and Faster Analyses

COSMOCORE 2.6C18 delivers performance equivalent to sub-2 µm particles at faster flow rate and analysis time while maintaining a lower back pressure. COSMOCORE can also be used in longer column size to gain additional resolution.

table

table

Condition: Column size; 2.1mm I.D. - 50 mm, Mobile phase; Acetonitrile / Water = 70 / 30, Temperature; 40°C, Sample; Amylbenzene

Higher retention and loading capacity than competitors' core-shell columns

Retention

COSMOCORE 2.6C18 features high retention and better separation compared to other core-shell columns.

Chromatograms

Condition
Column size 2.1 mm I.D. x 50 mm Sample
  1. Benzene
    (2.0 mg/ml)
  2. Toluene
    (2.0 mg/ml)
  3. Ethylbenzene
    (2.0 mg/ml)
  4. Propylbenzene
    (2.0 mg/ml)
  5. Butylbenzene
    (2.0 mg/ml)
  6. Amylbenzene
    (2.0 mg/ml)
Mobile phase: A: Acetonitrile / Water = 50 / 50
B: Acetonitrile / Water = 70 / 30
B conc. 0→100% (0-4 min), 100%(4-5 min)
Flow rate 0.4 ml/min
Temperature 40 °C
Detection UV254nm Inj.Vol. 0.5 µl

Low Bleed - Suitable for LC-MS

COSMOCORE 2.6C18 has low column bleed and consequently low MS noise level.

table

Condition
Column size 4.6 mm I.D. x 150 mm
Mobile phase A: 0.1% Formic Acid
B: Acetonitrile
B conc.
5→100% (0 - 5 min), 100% (5 -10 min),
100→5% (10 - 11 min), 5% (11 - 17 min)
Flow rate 0.2 ml/min
Temperature 40°C
Detection MS (TIC (+), MW 50-1000)

Applications

Sharp Peaks with Many Types of Compounds

COSMOCORE 2.6C18 features a special end capping treatment that effectively shields residual silanol groups, yielding sharp peaks for basic compounds and metal coordination complexes.

Basic compounds

Amitriptyline

Chromatograms
Condition
Column Size 2.1 mm I.D. x 50 mm
Mobile Phase Acetonitrile / 20mmol/l Phosphoric Acid
Buffer(pH7.0) = 60 / 40
Flow Rate 0.4 ml/min
Temperature 40°C
Detection UV254nm
Sample
  1. Amitriptyline
    (0.2 mg/ml)
  2. Amylbenzene
    (2.0 mg/ml)
Inj.Vol. 0.5 µl

Haloperidol

Haloperidol
Condition
Column Size 2.1 mm I.D. x 50 mm
Mobile Phase A; Acetonitrile/
20mmol/l Phosphate buffer(pH7.0)
= 10/90
B; Acetonitrile/
20mmol/l Phosphate buffer(pH7.0)
= 50/50
B conc. 0→100%(0→3min), 100%(3-5min)
Flow Rate 0.4 ml/min
Temperature 40°C
Detection UV220nm
Sample
  1. 4-(4-Chlorophenyl)-4-hydroxypiperidine (0.1 mg/ml)
  2. Haloperidol (0.1 mg/ml)
Inj.Vol. 0.5 µl

Procainamide

Procainamide
Condition
Column Size 2.1 mm I.D. x 50 mm
Mobile Phase Acetonitrile/ 20mmol/l Phosphate buffer(pH7.0)
= 10/90
Flow Rate 0.4 ml/min
Temperature 40°C
Detection UV270nm
Sample
  1. Procainamide (0.05 mg/ml)
  2. N-Acetylprocainamide (0.05 mg/ml)
Inj.Vol. 0.5 µl

Caffeine

Caffeine
Condition
Column Size 2.1 mm I.D. x 100 mm
Mobile Phase Acetonitrile/ H2O = 10/90
Flow Rate 0.4 ml/min
Temperature 40°C
Detection UV275nm
Sample
  1. Theobromine (0.05mg/ml)
  2. Theophylline (0.05mg/ml)
  3. Caffeine (0.05mg/ml)
Inj.Vol. 1.0 µl

Ciprofloxacin

Ciprofloxacin
Condition
Column Size 2.1 mm I.D. x 100 mm
Mobile Phase Acetonitrile/
20mmol/l Phosphate
buffer(pH2.5) = 10/90
Flow Rate 0.4 ml/min
Temperature 40°C
Detection UV278nm
Sample
  1. Ciprofloxacin　Ethylenediamine Analog
    (0.05 mg/ml)
  2. Ciprofloxacin
    (0.05 mg/ml)
Inj.Vol. 0.5 µl

Metal coordination complexes

Oxine-copper

Chromatograms
Condition
Column Size 2.1 mm I.D. x 50 mm
Mobile Phase Acetonitrile / 20mmol/l Phosphoric Acid
= 10 / 90
Flow Rate 0.4 ml/min
Temperature 40°C
Detection UV254nm
Sample
  1. Oxine-copper
    (0.003 mg/ml)
  2. Caffeine
    (0.3 mg/ml)
Inj.Vol. 0.5 µl

See more COSMOSIL Applications

Usage information

Column 2.6 COSMOCORE C18
I.D. (mm) 2.1 3 4.6
Washing method 1. Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. For example, if your mobile phase was 50:50 methanol/20 mmol/l phosphate buffer, wash with 50:50 methanol/water.

2. (If you used a buffer, always do step 1 first!) Remove adsorbed compounds and fix unstable baselines by washing with methanol and/or THF.
Storage conditions Short-term (up to a week):
Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. For example, if your mobile phase was 50:50 methanol/20 mmol/l phosphate buffer, wash with 50:50 methanol/water.

Long-term:
Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. Then, replace the solvent with 70% methanol or acetonitrile : 30% water.

In either case, tightly plug the column, and store in a cool and dry place.
Usable pH range pH1.5～pH10
(pH 2-7.5 recommended)
Max. pressure 60MPa
Temperature range The maximum usable temperature is 60°C. However. for regular use, please use at a constant temperature between 20°C and 50°C.
Usable solvents Any solvent that will not dissolve the silica gel (such as alkaline solutions) or cleave the stationary phase (such as very acidic solutions) is usable.
Note: For solvents with high viscosity, please watch the system pressure and keep it below 60 MPa. Also, please wash the column after using acidic mobile phases or solvents with high freezing points.
Other instructions - Buffer concentration is usually sufficient at 0.005 - 0.02 mol/L.
- Always filter mobile phases using a 0.45 µm or finer filter before use.
- Reproducibility may worsen when using a mobile phase that is more than 90% water.

Downloads

Brochures

Ordering Information

Analytical Column (Particle size : 2.6 µm)

COSMOCORE 2.6C18

Product Name Size Product No. Price
COSMOCORE 2.6C18
2.1 mm I.D. x 30 mm 12632-31 Inquire
Now
2.1 mm I.D. x 50 mm 12631-41
2.1 mm I.D. x 75 mm 12630-51
2.1 mm I.D. x 100 mm 12614-71
2.1 mm I.D. x 150 mm 12612-91
3.0 mm I.D. x 30 mm 12611-01
3.0 mm I.D. x 50 mm 12609-51
3.0 mm I.D. x 75 mm 12608-61
3.0 mm I.D. x 100 mm 12607-71
3.0 mm I.D. x 150 mm 12602-21
4.6 mm I.D. x 30 mm 12601-31
4.6 mm I.D. x 50 mm 12600-41
4.6 mm I.D. x 75 mm 12599-91
4.6 mm I.D. x 100 mm 12598-01
4.6 mm I.D. x 150 mm 12597-11
4.6 mm I.D. x 250 mm 12596-21

Other sizes may be available.

>> For UHPLC (COSMOCORE) fittings and adapters, please see this page.