Products

COSMOCORE 2.6Cholester

Features

Coreja

  • Cholesterol-bonded reversed-phase core-shell column
  • Usable under the same conditions as C18 columns
  • Better selectivity for cis-trans isomers, polyphenols, and natural products

>> Information on COSMOCORE 2.6C18

>> Information on COSMOCORE 2.6PBr

>> Information on COSMOSIL 5Cholester

Product Information

About Core-Shell Particles

About Core-Shell Particles

Column Specifications

Column Specifications

About Core-Shell 2.6Cholester Particles

COSMOCORE 2.6Cholester is packed with cholesterol-bonded 2.6 µm core-shell particles (Figure 1 and 2). It can achieve low pressure compared to fully-porous sub-2 µm columns (Figure 3).

Core-Shell Particle

Figure 1: Core-Shell Particle

Cholester

Figure 2: Bonded Cholestrol Stationary Phase

Comparison of Column Pressure

Figure 3: Comparison of Column Pressure

Column size 2.1mmI.D.-100mm, Mobile Phase Acetonitrile/Water=70/30, Temperature: 40°C

Comparison with C18

COSMOCORE 2.6Cholester offers improved separation for cis-trans isomers than C18 under typical reversed-phase mobile phase.

Application

Condition
Column size 2.1 mm I.D. - 100 mm Sample
  1. Vitamin K2 (0.50mg/ml)
  2. trans-Vitamin K1 (0.50mg/ml)
  3. cis-Vitamin K1
Mobile phase Methanol
Flow rate 0.4 ml/min
Temperature 40°C Inj. Vol 0.5µl
Detection UV280nm Structure

Separation Properties

COSMOCORE 2.6Cholester has about the same hydrophobicity as C18. It is not necessary to change the analytical conditions when replacing C18 Columns with COSMOCORE 2.6Cholester.

Application

Condition
Column size 2.1 mm I.D. - 100 mm Sample
  1. Uracil
  2. Methyl Benzoate
  3. Toluene
  4. Naphthalene
Mobile phase Acetonitrile/ H2O = 50/50
Flow rate 0.4 ml/min
Temperature 40°C
Detection UV254nm Inj. Vol 0.25µl

Molecular Shape Selectivity

COSMOCORE 2.6Cholester has excellent shape slectivity due to its structural rigidity. COSMOCORE 2.6Cholester retains planar triphenylene longer than nonplanar o-Terphenyl.

Application

Condition
Column size 2.1 mm I.D. - 100 mm Sample
  1. o-Terphenyl (0.25mg/ml)
  2. Triphenylene (0.05mg/ml)
Mobile phase Acetonitrile/ H2O = 70/30
Flow rate 0.4 ml/min Inj. Vol 0.5µl
Temperature 40°C Structure
Detection UV254nm

Scaling Up from Analytical to Preparative Separation

COSMOCORE 2.6Cholestrer has the same functional group as the fully-porous COSMOSIL Cholester 5 µm silica packing material. Therefore, the separation pattern in COSMOCORE 2.6Cholester is maintained when scaling up to preparative separation with 5 µm COSMOSIL Cholester.

Application

Condition
Mobile phase Methanol Sample
  • trans-Vitamin K1
  • cis-Vitamin K1
Flow rate (2.1mmI.D.) 0.4 ml/min
(4.6mmI.D.) 1.0 ml/min
(10.0mmI.D.) 5.0 ml/min
Temperature 40°C Structure
Detection UV280nm

References to COSMOSIL Cholesterol-BondedStationary Phase

The number of COSMOSIL Cholester reference has grown to over 100 since its introduction in 2005. (Reference Search: Google Scholar, as of July 2014)

Table Figure 1: The number of references to COSMOSIL Cholester and 2.5Cholester

Applications

Separation of natural components (Tomato components)

Lycopene exists in many cis-trans isomers. COSMOCORE 2.6Cholester separates these compounds better than C18.

Application

Condition Sample preparation
Column size 3.0mmI.D.-50mm Preparation Method
Mobile phase (2.6C18) Acetonitrile/ H2O = 95/5
(2.6Cholester) Acetonitrile
Flow rate 1.0 ml/min
Temperature 40°C
Detection UV470nm
Sample
  1. Lycopene
  2. β-Carotene
Inj. Vol 0.5µl

Separation of Catechin

Application

Condition
Column Size 2.1mmI.D.-50mm Sample
  1. (-)-Gallocatechin[(-)-GC]
  2. Caffeine
  3. (-)-Epigallocatechin[(-)-EGC]
  4. (-)-Catechin[(-)-C] 
  5. (-)-Epicatechin[(-)-EC]
  6. (-)-Epigallocatechin G allate[(-)-EGCg]
  7. (-)-Gallocatechin Gallate[(-)-GCg]
  8. (-)-Epicatechin Gallate[(-)-ECg]
  9. (-)-Catechin Gallat[(-)-Cg]
Mobile Phase A; Acetonitrile/ 0.1% H3PO4 = 10/90
B; Acetonitrile/ 0.1% H3PO4 = 40/60
B conc. 0 - 100% 3min Linear gradient
Flow Rate 0.6 ml/min
Temperature 30°C
Detection UV280nm

Data courtesy of a customer

Apply to LC/MS

LCMS Data

Condition
Column Size 3.0mmI.D.-50mm Detection APCI-MS,
Positive, SIM
Mobile Phase Acetonitrile Sample Tomato extract
  1. Lycopene
  2. β-Carotene
Flow Rate 1.0 ml/min
Temperature 40°C Inj.Vol. 0.5µl

icon See more COSMOSIL Applications

Usage information

Column 2.6 COSMOCORE Cholester
I.D. (mm) 2.1 3 4.6
Washing method 1. Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. For example, if your mobile phase was 50:50 methanol/20 mmol/l phosphate buffer, wash with 50:50 methanol/water.

2. (If you used a buffer, always do step 1 first!) Remove adsorbed compounds and fix unstable baselines by washing with methanol and/or THF.
Storage conditions Short-term (up to a week):
Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. For example, if your mobile phase was 50:50 methanol/20 mmol/l phosphate buffer, wash with 50:50 methanol/water.

Long-term:
Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. Then, replace the solvent with 70% methanol or acetonitrile : 30% water.

In either case, tightly plug the column, and store in a cool and dry place.
Usable pH range pH 2-7.5
Max. pressure 60MPa
Temperature range The maximum usable temperature is 60°C. However. for regular use, please use at a constant temperature between 20°C and 50°C.
Usable solvents Any solvent that will not dissolve the silica gel (such as alkaline solutions) or cleave the stationary phase (such as very acidic solutions) is usable.
Note: For solvents with high viscosity, please watch the system pressure and keep it below 60 MPa. Also, please wash the column after using acidic mobile phases or solvents with high freezing points.
Other instructions - Buffer concentration is usually sufficient at 0.005 - 0.02 mol/L.
- Always filter mobile phases using a 0.45 µm or finer filter before use.

Downloads

Brochures

  • COSMOCORE Series pdf(5.5 MB)
  • COSMOSIL/COSMOCORE Cholester Series Application Notebook and Reference List pdf (14 MB)

Posters

  • Identifying vitamins D2/D3 and their 25-OH metabolites and C3 epimers in a single LC-MS run (Poster from HPLC2017 Prague)
    pdf (0.3 MB)
  • Detecting THC Metabolites and other Cannabinoids (Poster from MSACL 2016 US)
    pdf (0.3 MB)
  • A Novel Core-Shell HPLC Column with Unique Shape Selectivity
    (Poster from EAS2014, Somerset, NJ USA)
    pdf (0.3 MB)

Ordering Information

Analytical Column (Particle size : 2.6 µm)

COSMOCORE 2.6Cholester Column

Product Name Size Product No. Price
COSMOCORE 2.6Cholester
2.1 mm I.D. x 30 mm 12858-91 Inquire
Now
2.1 mm I.D. x 50 mm 12859-81
2.1 mm I.D. x 75 mm 12860-41
2.1 mm I.D. x 100 mm 12861-31
2.1 mm I.D. x 150 mm 12862-21
3.0 mm I.D. x 30 mm 12863-11
3.0 mm I.D. x 50 mm 12864-01
3.0 mm I.D. x 75 mm 12866-81
3.0 mm I.D. x 100 mm 12867-71
3.0 mm I.D. x 150 mm 12868-61
4.6 mm I.D. x 30 mm 12869-51
4.6 mm I.D. x 50 mm 12870-11
4.6 mm I.D. x 75 mm 12871-01
4.6 mm I.D. x 100 mm 12872-91
4.6 mm I.D. x 150 mm 12873-81
4.6 mm I.D. x 250 mm 12875-61

Other sizes may be available.

>> For UHPLC (COSMOCORE) fittings and adapters, please see this page.