COSMOSIL HPLC Columns
Silica Based Preparative Columns
COSMOSIL series is available in 10.0 mm I.D. and 20.0 mm I.D. for semi-preparative applications and 28.0 mm I.D. and 50.0 mm I.D. for preparative scales. For column sizes and packing materials not listed below, contact either your local distributor or the manufacturer directly.
Material characteristics
| Packing material | 15C18-MS-II | 15C18-AR-II | 15C18-PAQ |
|---|---|---|---|
| Silica gel | high purity porous spherical silica | ||
| Average particle size | 15 m | ||
| Average pore size | approx. 120 | ||
| Specific surface area | approx. 300 m2/g | ||
| Stationary phase | octadecyl group | ||
| Bonding type | monomeric | polymeric | |
| Main interaction | hydrophobic interaction | ||
| End capping treatment | near-perfect treatment | ||
| Carbon content | approx. 16% | approx. 17% | approx. 11% |
| pH range | 2-10* | 1.5-7.5* | 2-7.5 |
| Feature | This phase is recommended for most of applications but particularly effective for basic organic compounds. | This phase is recommended for the separations requiring acidic mobile phase conditions. It also shows superior molecular shape selectivity to monomeric type C18columns. | This phase is designed to offer superior retention of polar compounds and excellent reproducibility in highly aqueous mobile phases, even in 100% aqueous. |
* Optimum pH range of columns based on silica gel is between 2 and 7.5.
Comparison of particle size
When change particle size of packing material from 5 m to 15 m, the number of theoretical plate (N) is reduced by one-third, and the pressure is reduced by one-ninth. As shown in the figures below, when a small amount of sample is injected, there is a big difference in the number of theoretical plates between 5 m and 15 m. However, when a large amount of sample is injected, there is not much difference between the two. Therefore the low pressure packing material (particle size 15 m) is recommended for preparative columns (28 mm I.D. or more).
The number of theoretical plate of N=4. Naphthalene peak.
| Condition | |||
|---|---|---|---|
| Column size | 4.6 mm I.D. x 250 mm | Detection | UV 254 nm |
| Mobile phase | methanol : water = 70 : 30 | Pressure | 5C18-AR-II/ 10.9 MPa 15C18-AR-II/1.8 MPa |
| Flow rate | 1.0 ml/min | Sample |
|
| Temperature | 30°C | ||
Preparative separation using 50.0 mm I.D. column
Carbazol is extracted from anthracene oil (coal tar) and required high purity because it is often used for analytical applications. Following is the preparative separation of carbazol using a 50.0 mm I.D. COSMOSIL 15C18-AR-Ⅱ .
| Condition | |
|---|---|
| Column | COSMOSIL 5C18-AR-II 4.6 mm I.D. x 150 mm |
| Flow rate | 0.5 ml/min |
| Temperature | room temperature |
| Detection | UV 254 nm |
| Condition | |
|---|---|
| Column | COSMOSIL 15C18-AR-II 50 mm I.D. x 250 mm |
| Flow rate | 60 ml/min |
| Temperature | room temperature |
| Detection | UV 254 nm |
Ordering information
Preparative column (Particle size : 15 m)
COSMOSIL 15C18-MS-II
| Product name | Size | Product No. |
|---|---|---|
| COSMOSIL 15C18-MS-II Guard Column | ||
| 28 mm l.D. x 50 mm | 05885-51 | |
| 50 mm l.D. x 50 mm | 34527-41 | |
| COSMOSIL 15C18-MS-II Packed Column | ||
| 28 mm l.D. x 250 mm | 34525-61 | |
| 50 mm l.D. x 250 mm | 05886-41 | |
| 50 mm l.D. x 500 mm | 34531-71 |
COSMOSIL 15C18-AR-II
| Product name | Size | Product No. |
|---|---|---|
| COSMOSIL 15C18-AR-II Guard Column | ||
| 28 mm l.D. x 50 mm | 38030-11 | |
| 50 mm l.D. x 50 mm | 38057-81 | |
| COSMOSIL 5CN-MS Packed Column | ||
| 28 mm l.D. x 250 mm | 37978-51 | |
| 50 mm l.D. x 250 mm | 38058-71 | |
| 50 mm l.D. x 500 mm | 05884-61 |
COSMOSIL 15C18-PAQ
| Product name | Size | Product No. |
|---|---|---|
| COSMOSIL 15C18-PAQ Guard Column | ||
| 28 mm l.D. x 50 mm | 05887-31 | |
| 50 mm l.D. x 50 mm | 05889-11 | |
| COSMOSIL 15C18-PAQ Packed Column | ||
| 28 mm l.D. x 250 mm | 05888-21 | |
| 50 mm l.D. x 250 mm | 05890-71 | |
| 50 mm l.D. x 500 mm | 05891-61 |
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