Products

COSMOSIL C18-PAQ

Features

Column Image

  • Compatible with 100% water based mobile phase
  • Suitable for hydrophilic compounds

Product Information

Column Specifications

Column Specifications

Stable Performance

The figure shows the change of retention time for thymine with 100% aqueous mobile phase (20 mmol/l phosphate buffer pH7). The sample was analyzed 4 times (1 hour). Flow of mobile phase was then stopped for 1 hour. The sample was analyzed under the same condition again after 1 hour. The conventional C18 column showed change of retention time, but COSMOSIL 5C18-PAQ maintained stable retention time.

Stable performance under 100% aqueous conditions

Stable_performance.gif

Applications

Organic Acids

Chromatogram
Condition
Column Size 4.6 mm I.D. x 250 mm
Mobile Phase 20mmol/l Phosphate buffer(pH2.5)
Flow Rate 1.0 ml/min
Temperature 30°C
Detection UV210nm
Sample
  1. Glycolic Acid (6.3µg)
  2. Tartaric Acid (4.0µg)
  3. L‐(-)-Malic Acid (6.4µg)
  4. Lactic Acid (13µg)
  5. Acetic Acid (13µg)
  6. Citric Acid(6.3µg)
  7. Succinic Acid (13µg)
  8. Maleic Acid (0.06µg)
  9. Propionic Acid(13µg)
  10. Fumaric Acid (0.03µg)

Nucleobases and Nucleosides

Chromatogram
Condition
Column Size 4.6 mm I.D. x 150 mm
Mobile Phase 20mmol/l Phosphate buffer(pH7)
Flow Rate 1.0 ml/min
Temperature 30°C
Detection UV260nm
Sample
  1. Cytosine (0.16µg)
  2. Uracil (0.16µg)
  3. Cytidine (0.32µg)
  4. Uridine (0.24µg)
  5. Guanine (0.16µg)
  6. Thymine (0.16µg)
  7. Adenine (0.08µg)
  8. Guanosine (0.24µg)
  9. Thymidine(0 24µg)
  10. Adenosine (0.16µg)

dNTP

Chromatogram
Condition
Column Size 4.6 mm I.D. x 250 mm
Mobile Phase 20mmol/l Phosphate buffer(pH7)
Flow Rate 1.0 ml/min
Temperature 30°C
Detection UV260nm, 0.16AUFS
Sample
  1. 2’-Deoxycytidine 5’-Triphosphate [dCTP] (1mmol/l)
  2. 2’-Deoxythymidine 5’-Triphosphate [dTTP] (1mmol/l)
  3. 2’-Deoxyguanosine 5’-Triphosphate [dGTP] (1mmol/l)
  4. 2’-Deoxyadenosine 5’-Triphosphate [dATP] (1mmol/l)
Injection 0.5µl

Catecholamines

Chromatogram
Condition
Column Size 4.6 mm I.D. x 250 mm
Mobile Phase 20mmol/l Phosphate buffer(pH7.0)
Flow Rate 1.0 ml/min
Temperature 30°C
Detection UV270nm
Sample
  1. L-Noradrenaline (0.38µg)
  2. (±)-Epinephrine (0.38µg)
  3. DL-3-Methoxy
    -4-hydroxymandelic Acid (0.56µg)
  4. L-DOPA (0.56µg)
  5. Dopamine (1.13µg)
  6. Homovanillic Acid (1.13µg)

Ascorbic Acid

Chromatogram
Condition
Column Size 4.6 mm I.D. x 250 mm
Mobile Phase 20mmol/l Phosphoric Acid
Flow Rate 1.0 ml/min
Temperature 30°C
Detection UV245nm, 0.16AUFS
Sample L(+)-Ascorbic Acid [Vitamin C] (0.1µg)

2-Phenylethyl glycosides

Chromatogram
Condition
Column Size 4.6 mm I.D. x 150 mm
Mobile Phase Acetonitrile/Methanol/ H2O= 8/4/88
Flow Rate 1.0 ml/min
Temperature 30°C
Detection UV210nm
Sample
  1. 2-Phenylethyl-β-melibioside
  2. 2-Phenylethyl-β-gentiobioside
  3. 2-Phenylethyl-β-lactoside
  4. 2-Phenylethyl-β-cellobioside
  5. 2-Phenylethyl-β-maltoside

See more COSMOSIL Applications

Usage information

Column 5 µm C18-PAQ
I.D. (mm) 1.0 2.0 3.0 4.6 6.0 10.0 20.0 28.0
Washing method 1. Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. For example, if your mobile phase was 50:50 methanol/20 mmol/l phosphate buffer, wash with 50:50 methanol/water.
2. (If you used a buffer, always do step 1 first!) Remove adsorbed compounds and fix unstable baselines by washing with methanol and/or THF.
Storage conditions Short-term (up to a week):
Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. For example, if your mobile phase was 50:50 methanol/20 mmol/l phosphate buffer, wash with 50:50 methanol/water.

Long-term:
Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. Then, replace the solvent with 70% methanol or acetonitrile : 30% water.

In either case, tightly plug the column, and store in a cool and dry place.
Usable pH range pH2~pH7.5
Max. pressure 20MPa 15MPa
Temperature range The maximum usable temperature is 60°C. However. for regular use, please use at a constant temperature between 20°C and 50°C.
Usable solvents Any solvent that will not dissolve the silica gel (such as alkaline solutions) or cleave the stationary phase (such as very acidic solutions) is usable.
Note: For solvents with high viscosity, please watch the system pressure and keep it below 20 MPa. Also, please wash the column after using acidic mobile phases or solvents with high freezing points.
Other instructions - Buffer concentration is usually sufficient at 0.005 - 0.02 mol/L.
- Always filter mobile phases using a 0.5 µm or finer filter before use.

Downloads

Brochure

COSMOSIL 5C18-PAQpdf (659 KB)

References

Reference list

Ordering Information

Analytical Column (Particle Size : 5 µm)
COSMOSIL 5C18-PAQ

Product Name Size Product No. Price
COSMOSIL 5C18-PAQ Guard Column
4.6 mm I.D. x 10 mm 02484-91 e-Nacalai
10 mm I.D. x 20 mm 34457-61
20 mm I.D. x 20 mm 05803-11
20 mm I.D. x 50 mm 05804-01
28 mm I.D. x 50 mm 34455-81
COSMOSIL 5C18-PAQ Packed Column
1.0 mm I.D. x 50 mm 05792-61 e-Nacalai
1.0 mm I.D. x 150 mm 05793-51
2.0 mm I.D. x 30 mm 05878-51
2.0 mm I.D. x 50 mm 05794-41
2.0 mm I.D. x 100 mm 05470-71
2.0 mm I.D. x 150 mm 34449-71
2.0 mm I.D. x 250 mm 05795-31
3.0 mm I.D. x 100 mm 05796-21
3.0 mm I.D. x 150 mm 05797-11
3.0 mm I.D. x 250 mm 05798-01
4.6 mm I.D. x 30 mm 05879-41
4.6 mm I.D. x 50 mm 34451-21
4.6 mm I.D. x 100 mm 05799-91
4.6 mm I.D. x 150 mm 02486-71
4.6 mm I.D. x 250 mm 02485-81
6.0 mm I.D. x 150 mm 34419-61
6.0 mm I.D. x 250 mm 05800-41
10 mm I.D. x 50 mm 05801-31
10 mm I.D. x 150 mm 34466-41
10 mm I.D. x 250 mm 34376-21
20 mm I.D. x 150 mm 34476-11
20 mm I.D. x 250 mm 34373-51
28 mm I.D. x 250 mm 34456-71

Preparative Column (Particle Size : 15 µm)
COSMOSIL 15C18-PAQ

Product Name Size Product No. Price
COSMOSIL 15C18-PAQ Guard Column
28 mm l.D. x 50 mm 05887-31 e-Nacalai
50 mm l.D. x 50 mm 05889-11
COSMOSIL 15C18-PAQ Packed Column
28 mm l.D. x 250 mm 05888-21 e-Nacalai
50 mm l.D. x 250 mm 05890-71
50 mm l.D. x 500 mm 05891-61

(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze