COSMOSIL Index

COSMOSIL 5C18-PAQ

The COSMOSIL 5C18-PAQ is a new member of our C18 column family which already includes the monomeric type MS-II and the polymeric type AR-II. This new column maintains stable retention time even in 100% aqueous mobile phases. The new polymeric linking style gives this column a strong acidic resistance so that it is compatible with mobile phases of acidic pH that can permanently damage conventional octadecyl stationary phases. This type is especially good for separation of hydrophilic compounds.

Stable Performance under 100% Aqueous Conditions

The figure shows the change of retention time for thymine with 100% aqueous mobile phase (20 mmol/l phosphate buffer pH7). The sample was analyzed 4 times. Flow of mobile phase was then stopped for 1 hour. The sample was analyzed under the same condition again after 1 hour. The conventional C18 column showed change of retention time, but COSMOSIL 5C18-PAQ maintained stable retention time.

Stable_performance.gif

Applications

Organic Acid

Organic Acid

Condition
Column Size 4.6 mm I.D. x 250 mm
Mobile Phase 20 mmol/l Phosphoric Acid
Flow rate 1.0ml/min
Temperature 30°C
Detection UV 210 nm 0.16AUFS
Sample
  1. Glycolic Acid 6.3 µg
  2. Malic Acid 6.3 µ g
  3. Lactic Acid 13 µg
  4. Citric Acid 6.3 µ g
  5. Maleic Acid 0.063 µg
  6. Fumaric Acid 0.031 µg

Organic Acid

Condition
Column Size 4.6 mm I.D. x 250 mm
Mobile Phase 20 mmol/l Phosphoric Acid
Flow Rate 1.0ml/min
Temperature 30°C
Detection UV 210 nm 0.16AUFS
Sample
  1. Tartaric Acid 4.0 µg
  2. Acetic Acid 13 µg
  3. Succinic Acid 13 µg
  4. Propionic Acid 13 µg
Nucleic-acid Base

Nucleic-acid base

Condition
Column Size 4.6 mm I.D. x 150 mm
Mobile Phase 20 mmol/l Phosphate Buffer (pH7)
Flow Rate 1.0 ml/min
Temperature 30°C
Detection UV 254 nm 0.16AUFS
Sample
  1. Cytosine 0.1 µg
  2. Uracil 0.1 µg
  3. Guanine 0.1 µg
  4. Thymine 0.2 µg
  5. Adenine 0.05 µg
dNTP

dNTP

Condition
Column Size 4.6 mm I.D. x 250 mm
Mobile Phase 20 mmol/l Phosphate Buffer (pH7)
Flow Rate 1.0 ml/min
Temperature 30°C
Detection UV 260 nm 0.16 AUFS
Sample
  1. dCTP
  2. dTTP
  3. dGTP
  4. dATP 1 mmol/ each 0.5 µl
Ascorbic Acid

Ascorbic Acid

Condition
Column Size 4.6 mm I.D. x 250 mm mm
Mobile Phase 20 mmol/l Phosphoric Acid
Flow Rate 1.0 ml/min
Temperature 30°C
Detection UV 245 nm 0.16 AUFS
Sample Ascorbic Acid (Vitamin C) 0.1 µg
2-Phenylethyl Glycoside

2-Phenylethyl glycoside

2-Phenylethyl glycoside

Condition
Column Size 4.6 mm I.D. x 150 mm
Mobile Phase Acetonitrile : Methanol : Water = 8 : 4 : 88
Flow Rate 1.0 ml/min
Temperature 30°C
Detection UV 210 nm 0.064 AUFS
Sample 10.5 MPa

Ordering Information

Analytical Column (Particle Size : 5 µm)
COSMOSIL 5C18-PAQ
Product Name Size Product No.
COSMOSIL 5C18-PAQ Guard Column
4.6 mm I.D. x 10 mm 02484-91
10 mm I.D. x 20 mm 34457-61
20 mm I.D. x 20 mm 05803-11
20 mm I.D. x 50 mm 05804-01
28 mm I.D. x 50 mm 34455-81
COSMOSIL 5C18-PAQ Packed Column
1.0 mm I.D. x 50 mm 05792-61
1.0 mm I.D. x 150 mm 05793-51
2.0 mm I.D. x 30 mm 05878-51
2.0 mm I.D. x 50 mm 05794-41
2.0 mm I.D. x 100 mm 05470-71
2.0 mm I.D. x 150 mm 34449-71
2.0 mm I.D. x 250 mm 05795-31
3.0 mm I.D. x 100 mm 05796-21
3.0 mm I.D. x 150 mm 05797-11
3.0 mm I.D. x 250 mm 05798-01
4.6 mm I.D. x 30 mm 05879-41
4.6 mm I.D. x 50 mm 34451-21
4.6 mm I.D. x 100 mm 05799-91
4.6 mm I.D. x 150 mm 02486-71
4.6 mm I.D. x 250 mm 02485-81
6.0 mm I.D. x 150 mm 34419-61
6.0 mm I.D. x 250 mm 05800-41
10 mm I.D. x 50 mm 05801-31
10 mm I.D. x 150 mm 34466-41
10 mm I.D. x 250 mm 34376-21
20 mm I.D. x 150 mm 34476-11
20 mm I.D. x 250 mm 34373-51
28 mm I.D. x 250 mm 34456-71
Preparative Column (Particle Size : 15 µm)
COSMOSIL 15C18-PAQ
Product Name Size Product No.
COSMOSIL 15C18-PAQ Guard Column
28 mm l.D. x 50 mm 05887-31
50 mm l.D. x 50 mm 05889-11
COSMOSIL 15C18-PAQ Packed Column
28 mm l.D. x 250 mm 05888-21
50 mm l.D. x 250 mm 05890-71
50 mm l.D. x 500 mm 05891-61

▲Page top