COSMOSIL Cholester
Features
- Cholesterol bonded stationary phase
- Usable under condition the same as C18
- Increased stereoselectivity and improved resolution for geometric isomers
- For separation of natural compounds
For information on COSMOCORE 2.6Cholester (2.6 µm Core Shell Particle)
Product Information
Column Specifications
Hydrophobic Interaction
Figure shows the comparison of hydrophobic interactions with competitive C18 columns. Cholester provides the same hydrophobicity as alkyl group bonded types (C18, C30). It is not necessary to change the analytical conditions when replacing C18 or C30 columns with Cholester.
Condition | |||
---|---|---|---|
Mobile phase | Methanol/H2O = 80/20 | Sample |
|
Flow rate | 1.0ml/min | ||
Temperature | 30°C | ||
Detection | UV 254 nm |
Comparison of Molecular Shape Selectivity
The stationary phase of Cholester has very rigid structures and can distinguish different molecular shapes. Cholester offers improved separation for structurally similar compounds that are difficult to analyze with alkyl group bonded materials (C18 and C30). As in the following example Cholester retains planar Triphenylene longer than stereoscopic o-Terphenyl.
Figure. Comparison of molecular sharp selectivity
Condition | |
---|---|
Column Size | 4.6 mm I.D. x 150 mm |
Mobile phase | Methanol : Water = 90 : 10 |
Flow rate | 1.0 ml/min |
Temperature | 30 °C |
Detection | UV 254 nm |
Sample | 1. o-Terphenyl 0.10 µg 2. Triphenylene 0.01 µg |
Improvement in Separation
COSMOSIL Cholester provides enhanced selectivity over traditional C18 columns and offers greater performance in separating isomers or other closely related compounds. COSMOSIL Cholester is ideal for method development and serves as an excellent alternative to traditional C18 columns. The figure below shows analytical data of chloroacetophenone isomers. These isomers are difficult to separate with C18 and C30, but they are well resolved by COSMOSIL Cholester.
Efficiency of Preparative Separation
The figure below shows the comparison of efficiency of preparative separation with a C18 column. Both columns show good separation. However, sample loading capacity for preparative separations can be affected by a slight difference in separation ability. COSMOSIL Cholester can load 4 times of sample volume compared with C18 columns.
Condition | |||
---|---|---|---|
Column Size | 4.6 mm I.D. x 150 mm | Detection | UV 254 nm |
Mobile phase | Methanol : Water = 100 : 0 | Sample |
|
Flow rate | 1.0ml/min | ||
Temperature | 30°C |
Applications
Catechins
Condition | |||
---|---|---|---|
Column Size | 4. 6mm I.D. x 150 mm | Sample |
Injection Vol. 1.0μl |
Mobile Phase | A: Acetonitrile/ 20mmol/l Phosphate buffer(pH2.5) = 10/90 B: Acetonitrile/ 20mmol/l Phosphate buffer(pH2 5) = 30/70 B conc. 0→100% 20min Linear gradient |
||
Flow Rate | 1.0 ml/min | ||
Temperature | 30°C | ||
Detection | UV280nm |
Flavones
Condition | |||
---|---|---|---|
Column Size | 4. 6mm I.D. x 150 mm | Sample |
|
Mobile Phase | A: Acetonitrile/ 20mmol/l Phosphate buffer(pH2 5) = 20/80 B: Acetonitrile/ 20mmol/l Phosphate buffer(pH2.5) = 70/30 B conc. 0→100% 20min Linear gradient |
||
Flow Rate | 1.0 ml/min | ||
Temperature | 30°C | ||
Detection | UV280nm |
Usage information
Column | 5 µm Cholester | |||||
---|---|---|---|---|---|---|
I.D. (mm) | 2.0 | 3.0 | 4.6 | 10.0 | 20.0 | 28.0 |
Washing method | 1. Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. For example, if your mobile phase was 50:50 methanol/20 mmol/l phosphate buffer, wash with 50:50 methanol/water. 2. (If you used a buffer, always do step 1 first!) Remove adsorbed compounds and fix unstable baselines by washing with methanol and/or THF. |
|||||
Storage conditions | Short-term (up to a week): Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. For example, if your mobile phase was 50:50 methanol/20 mmol/l phosphate buffer, wash with 50:50 methanol/water. Long-term: Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. Then, replace the solvent with 70% methanol or acetonitrile : 30% water. In either case, tightly plug the column, and store in a cool and dry place. |
|||||
Usable pH range | pH2~pH7.5 | |||||
Max. pressure | 20MPa | 15MPa | ||||
Temperature range | The maximum usable temperature is 60°C. However. for regular use, please use at a constant temperature between 20°C and 50°C. | |||||
Usable solvents | Any solvent that will not dissolve the silica gel (such as alkaline solutions) or cleave the stationary phase (such as very acidic solutions) is usable. Note: For solvents with high viscosity, please watch the system pressure and keep it below 20 MPa. Also, please wash the column after using acidic mobile phases or solvents with high freezing points. |
|||||
Other instructions | - Buffer concentration is usually sufficient at 0.005 - 0.02 mol/L. - Always filter mobile phases using a 0.5 µm or finer filter before use. |
Downloads
Brochures
- COSMOSIL Cholester (640 KB)
- COSMOSIL 2.5Cholester (1.2 MB)
- Analysis of Natural Compounds by 2.5Cholester (390 KB)
- Analysis of New Antimycotic Substances (5aTHQs) by COSMOSIL Cholester (1 MB)
- COSMOSIL/COSMOCORE Cholester Series Application Notebook and Reference List (14 MB)
Posters
- Identifying Vitamin D2 and D3 and their 25-OH Metabolites and C3 Epimers
in a Single LC-MS Run (Poster from Food Science 2016, Singapore)
(0.2 MB) - Simultaneous Separation of Fat-Soluble Vitamins by Reversed-Phase HPLC Using a Cholesteryl Group Bonded Stationary Phase (Poster from AOAC Annual Meeting & Exposition 2013, Chicago, IL USA)
(0.2 MB) - Rapid HPLC Method Development with C18 and Phenyl Columns
(Poster from HPLC2013, Amsterdam, the Netherlands)
(0.2 MB) - Simultaneous Separation of Fat-Soluble Vitamins by Reversed-Phase HPLC
Using a Cholesteryl Group Bonded Stationary Phase
(Poster from AOAC Annual Meeting 2012, Las Vegas, Nevada USA)
(0.2 MB)
References
Reference list
Ordering Information
Analytical Column (Particle Size : 5 µm)
COSMOSIL Cholester
Product Name | Size | Product No. | Price |
---|---|---|---|
COSMOSIL Cholester Guard Column | |||
4.6 mm I.D. x 10 mm | 05975-71 | ||
10 mm I.D. x 20 mm | 05978-41 | ||
20 mm I.D. x 20 mm | 05980-91 | ||
20 mm I.D. x 50 mm | 05981-81 | ||
28 mm I.D. x 50 mm | 05983-61 | ||
COSMOSIL Cholester Guard Cartridge Guard cartridge holder is required. |
4.6 mm l.D. x 10 mm | 19183-24 | |
COSMOSIL Cholester Packed Column | |||
1.0 mm I.D. x 150 mm | 05968-71 | ||
1.0 mm I.D. x 250 mm | 05969-61 | ||
2.0 mm I.D. x 150 mm | 05971-11 | ||
2.0 mm I.D. x 250 mm | 05972-01 | ||
3.0 mm I.D. x 150 mm | 05973-91 | ||
3.0 mm I.D. x 250 mm | 05974-81 | ||
4.6 mm I.D. x 50 mm | 06359-21 | ||
4.6 mm I.D. x 100 mm | 06591-61 | ||
4.6 mm I.D. x 150 mm | 05976-61 | ||
4.6 mm I.D. x 250 mm | 05977-51 | ||
10 mm I.D. x 150 mm | 08011-91 | ||
10 mm I.D. x 250 mm | 05979-31 | ||
20 mm I.D. x 150 mm | 06088-71 | ||
20 mm I.D. x 250 mm | 05982-71 | ||
28 mm I.D. x 250 mm | 05985-41 |
Analytical Column (Particle Size : 3 µm)
COSMOSIL 3Cholester
Product Name | Size | Product No. | Price |
---|---|---|---|
COSMOSIL 3Cholester Guard Cartridge Guard cartridge holder is required. |
2.0 mm I.D. × 10 mm | 19325-64 | |
4.6 mm I.D. × 10 mm | 19344-14 | ||
COSMOSIL 3Cholester Packed Column | 2.0 mm I.D. × 100 mm | 19190-11 | |
2.0 mm I.D. × 150 mm | 19191-01 | ||
2.0 mm I.D. × 250 mm | 19192-91 | ||
2.0 mm I.D. × 50 mm | 19188-61 | ||
2.0 mm I.D. × 75 mm | 19189-51 | ||
3.0 mm I.D. × 100 mm | 19196-51 | ||
3.0 mm I.D. × 150 mm | 19197-41 | ||
3.0 mm I.D. × 250 mm | 19198-31 | ||
3.0 mm I.D. × 50 mm | 19194-71 | ||
3.0 mm I.D. × 75 mm | 19195-61 | ||
4.6 mm I.D. × 100 mm | 19300-61 | ||
4.6 mm I.D. × 150 mm | 19151-21 | ||
4.6 mm I.D. × 250 mm | 19316-71 | ||
4.6 mm I.D. × 50 mm | 19199-21 | ||
4.6 mm I.D. × 75 mm | 19200-71 |
Ultra-High Performance Column (Particle Size : 2.5 µm)
COSMOSIL 2.5Cholester
For information on COSMOSIL 2.5Cholester (Ultra-High Performance Column)
(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze