COSMOSIL® Index
Ion Exchange Chromatography - COSMOGEL IEX Series Columns
| · Available in 3 different ion-exchange modes (Anion-exchange type, Cation-exchange type, Amphoteric ion-exchange type) · Available for 3 different application areas (for Purification, for Ultra-fast analysis, for Precise analysis) · For separation of biopolymers such as proteins or nucleic acids |
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COSMOGEL IEX Series
| Ion Exchange Mode | Anion-exchange Type | Cation-exchange Type | Amphoteric ion-exchange Type | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Packing Material | Type Q | Type Q-N | Type S | Type S-N | Type M | Type M-N | |||
| Gel / Average Particle Size | Hydrophilic Polymer / 5 µm | ||||||||
| Average Pore Size | 1000 Å | Non-porous | 1000 Å | Non-porous | 1000 Å | Non-porous | |||
| Functional Group | -CH3N+(CH3)3 | -(CH2)3SO3- | -CH3N+(CH3)3 + -(CH2)3SO3- |
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| Protein Binding Capacity | 110-150 mg | 12-20 mg | 70-100 mg | 10-18 mg | 55-75 mg(BSA)/ml 35-50 mg(IgG)/ml |
6-10 mg(BSA)/ml 5-9 mg(IgG)/ml |
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| BSA/ml-resin | human IgG/ml-resin | ||||||||
| Target Sample | Acidic Proteins / DNA | Basic Proteins | All Proteins | ||||||
| Application | For Purification | For Ultra-fast Analysis |
For Precise Analysis |
For Purification | For Ultra-fast Analysis |
For Precise Analysis |
For Purification | For Precise Analysis |
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| Column Size I.D. x Length(mm) |
4.6 x 50 | 4.6 x 30 | 4.6 x 100 | 4.6 x 50 | 4.6 x 30 | 4.6 x 100 | 4.6 x 50 | 4.6 x 100 | |
Comprehensive isolation of proteins by amphoteric ion-exchange type (Type M)
The amphoteric ion-exchange type enables the simultaneous separation of both acidic and basic proteins in one application.
| Type Q | Type S | Type M |
|---|---|---|
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| Condition | |||
|---|---|---|---|
| Column | COSMOGEL IEX (4.6 mm I.D. x 50 mm) | ||
| Mobile phase | A: 20 mmol/l Phosphate buffer (pH6.8) B: 20 mmol/l Phosphate buffer (pH6.8) + 0.5 mol /l NaCl B: conc. 0→100% 30 min Linear gradient |
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| Flow rate | 0.5 ml/min | ||
| Temperature | 30°C | ||
| Detection | UV 220 nm | ||
| Sample |
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Separation by different ion-exchange mode of amphoteric ion-exchange type (Type M)
Amphoteric ion-exchange type can be used as anion-exchange type or cation-exchange type.| Type Q | Type M |
|---|---|
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| Condition | |||
|---|---|---|---|
| Column | COSMOGEL IEX (4.6 mm I.D. x 50 mm) | ||
| Mobile phase | A: 20mmol/l Tris buffer (pH8.3) B: 20mmol/l Tris buffer (pH8.3) + 0.5mol/l NaCl B conc. 0→100% 30 min Linear Gradient |
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| Flow rate | 0.5 ml/min | ||
| Temperature | 30°C | ||
| Detection | UV 220 nm | ||
| Sample |
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Application Types
COSMOGEL IEX Series are available for 3 types of applications: purification, ultra-fast analysis and precise analysis.For Purification: Type Q, Type S, Type M
(Packing Material: Porous, Column Size: 4.6 mm I.D. x 50 mm)
Type Q, Type S and Type M with porous packing materials have higher binding
capacity of proteins than the respective non-porous type, which means that
peak shape does not spread even with injection of a large volume of sample.
Therefore they are highly suitable for purification of large sample.
| Condition | |
|---|---|
| Column | COSMOGEL IEX Type S (4.6 mm I.D. x 50 mm) |
| Mobile phase | A: 20mmol/l Phosphate buffer (pH6.8) B: 20mmol/l Phosphate buffer (pH6.8) + 0.5mol/l NaCl B conc. 0→100% 30min Linear Gradient |
| Flow rate | 0.5 ml/min |
| Temperature | 30°C |
| Detection | UV 280 nm |
| Sample | 1. Ribonuclease A 2. Cytochrome C 3. Lysozyme |
For Precise Analysis: Type Q-N, Type S-N, Type M-N (Packing Material: Non-porous, Column Size: 4.6 mm I.D. x 100 mm)
Type Q-N, Type S-N, Type M-N with non-porous packing materials reduce spreading of samples in packing materials, result in high resolution separation for precise analysis such as quality control of antibody drugs. The longer column length also contributes to the sharper peaks.
| Type S (4.6 mm I.D. x 50 mm) | Type S-N (4.6 mm I.D. x 100 mm) |
|---|---|
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| Condition | |||
|---|---|---|---|
| Column | COSMOGEL IEX | ||
| Mobile phase | A: 20mmol/l Phosphate buffer (pH6.8) B: 20mmol/l Phosphate buffer (pH6.8) + 0.5mol/l NaCl B conc. 0→100% Linear Gradient |
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| Flow rate | 0.5ml/min | ||
| Temperature | 30°C | ||
| Detection | UV 220 nm | ||
| Sample |
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For Ultra-fast Analysis: Type Q-N, Type S-N, Type M-N
(Packing Material: Non-porous, Column Size: 4.6 mm I.D. x 30 mm)
The resolution with non-porous packing materials is not much affected by high flow rate and thus the materials are suitable for fast analysis. The shorter column length contributes to the fast analysis.
| Type S (4.6 mm I.D. x 50 mm) | Type S-N (4.6 mm I.D. x 30 mm) |
|---|---|
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| Condition | |||
|---|---|---|---|
| Column | COSMOGEL IEX | ||
| Mobile phase | A: 20mmol/l Phosphate buffer (pH6.8) B: 20mmol/l Phosphate buffer (pH6.8) + 0.5mol/l NaCl B conc. 0→100% Linear Gradient Type S: 30min Type S-N: 10min |
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| Flow rate | Type S: 0.5ml/min Type S-N: 1.0ml/min | ||
| Temperature | 30°C | ||
| Detection | UV 220 nm | ||
| Sample |
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Ordering information
| Ion Exchange Mode | Product Name | Application | Size I.D. x Length (mm) |
Product Number |
|---|---|---|---|---|
| Anion-exchange Type | COSMOGEL IEX Type Q |
For Purification | 4.6 x 50 | 06266-31 |
| COSMOGEL IEX Type Q-N |
For Ultra-fast Analysis | 4.6 x 30 | 06264-51 | |
| COSMOGEL IEX Type Q-N |
For Precise Analysis | 4.6 x 100 | 06258-41 | |
| Cation-exchange Type | COSMOGEL IEX Type S |
For Purification | 4.6 × 50 | 06252-01 |
| COSMOGEL IEX Type S-N |
For Ultra-fast Analysis | 4.6 x 30 | 06251-11 | |
| COSMOGEL IEX Type S-N |
For Precise Analysis | 4.6 × 100 | 06250-21 | |
| Amphoteric ion-exchange type | COSMOGEL IEX Type M |
For Purification | 4.6 x 50 | 06248-71 |
| COSMOGEL IEX Type M-N |
For Precise Analysis | 4.6 x 100 | 06244-11 |
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