Products

COSMOSIL IEX Series

Features

IEX Image

  • Available in 3 different ion-exchange modes
    (Anion-exchange type, Cation-exchange type, Amphoteric ion-exchange type)
  • Available for 3 different application areas
    (for Purification, for Ultra-fast analysis, for Precise analysis)
  • For separation of biopolymers such as proteins or nucleic acids

Product Information

Ion Exchange Mode Anion-exchange Type Cation-exchange Type Amphoteric ion-exchange Type
Packing Material Type Q Type Q-N Type S Type S-N Type M Type M-N
Gel / Average Particle Size Hydrophilic Polymer / 5 µm
Average Pore Size 1000 Å Non-porous 1000 Å Non-porous 1000 Å Non-porous
Functional Group -CH3N+(CH3)3 -(CH2)3SO3- -CH3N+(CH3)3
+ -(CH2)3SO3-
Protein Binding Capacity 110-150 mg 12-20 mg 70-100 mg 10-18 mg 55-75 mg(BSA)/ml
35-50 mg(IgG)/ml
6-10 mg(BSA)/ml
5-9 mg(IgG)/ml
BSA/ml-resin human IgG/ml-resin
Target Sample Acidic Proteins / DNA Basic Proteins All Proteins
Application For Purification For
Ultra-fast
Analysis
For Precise
Analysis
For Purification For
Ultra-fast
Analysis
For Precise
Analysis
For Purification For Precise
Analysis
Column Size
I.D. x Length(mm)
4.6 x 50 4.6 x 30 4.6 x 100 4.6 x 50 4.6 x 30 4.6 x 100 4.6 x 50 4.6 x 100

Comprehensive Isolation of Proteins by Amphoteric Ion-exchange Type (Type M)

The amphoteric ion-exchange type enables the simultaneous separation of both acidic and basic proteins in one application.

Type Q Type S Type M
Chromatogram Chromatogram Chromatogram
Condition
Column COSMOGEL IEX (4.6 mm I.D. x 50 mm)
Mobile Phase A: 20 mmol/l Phosphate Buffer (pH6.8)
B: 20 mmol/l Phosphate Buffer (pH6.8) + 0.5 mol /l NaCl
B: conc. 0→100% 30 min Linear Gradient
Flow Rate 0.5 ml/min
Temperature 30°C
Detection UV 220 nm
Sample 1.  Ribonuclease A   
2. Cytochrome C    
3. Trypsin Inhibitor   
4.  β-Galactosidase 
(1.5 µg)
(1.5 µg)
(6.0 µg)
(6.0 µg)

Application Types

COSMOGEL IEX Series are available for 3 types of applications: purification, ultra-fast analysis and precise analysis.

For Purification: Type Q, Type S, Type M
(Packing Material: Porous, Column Size: 4.6 mm I.D. x 50 mm)

Type Q, Type S and Type M with porous packing materials have higher binding capacity of proteins than the respective non-porous type, which means that peak shape does not spread even with injection of a large volume of sample. Therefore they are highly suitable for purification of large sample.

Type SFigure

Condition
Column COSMOGEL IEX Type S (4.6 mm I.D. x 50 mm)
Mobile Phase A: 20mmol/l Phosphate Buffer (pH6.8)
B: 20mmol/l Phosphate Buffer (pH6.8) + 0.5mol/l NaCl
B conc. 0→100% 30min Linear Gradient
Flow Rate 0.5 ml/min
Temperature 30°C
Detection UV 280 nm
Sample 1. Ribonuclease A
2. Cytochrome C
3. Lysozyme

For Precise Analysis: Type Q-N, Type S-N, Type M-N (Packing Material: Non-porous, Column Size: 4.6 mm I.D. x 100 mm)

Type Q-N, Type S-N, Type M-N with non-porous packing materials reduce spreading of samples in packing materials, result in high resolution separation for precise analysis such as quality control of antibody drugs. The longer column length also contributes to the sharper peaks.

Type S (4.6 mm I.D. x 50 mm) Type S-N (4.6 mm I.D. x 100 mm)
Chromatogram Chromatogram
Condition
Column COSMOGEL IEX
Mobile Phase A: 20mmol/l Phosphate Buffer (pH6.8)
B: 20mmol/l Phosphate Buffer (pH6.8) + 0.5 mol/l NaCl
B conc. 0→100% Linear Gradient
Flow Rate 0.5ml/min
Temperature 30°C
Detection UV 220 nm
Sample 1. Ribonuclease A
2. Cytochrome C
3. Lysozyme 
(1.65 µg)
(1.65 µg)
(1.65 µg)

For Ultra-fast Analysis: Type Q-N, Type S-N, Type M-N
(Packing Material: Non-porous, Column Size: 4.6 mm I.D. x 30 mm)

The resolution with non-porous packing materials is not much affected by high flow rate and thus the materials are suitable for fast analysis. The shorter column length contributes to the fast analysis.

Type S (4.6 mm I.D. x 50 mm) Type S-N (4.6 mm I.D. x 30 mm)
Type S Type S-N
Condition
Column COSMOGEL IEX
Mobile Phase A: 20mmol/l Phosphate Buffer (pH6.8)
B: 20mmol/l Phosphate Buffer (pH6.8) + 0.5 mol/l NaCl
B conc. 0→100% Linear Gradient
Type S: 30min Type S-N: 10 min
Flow Rate Type S: 0.5ml/min Type S-N: 1.0ml/min
Temperature 30°C
Detection UV 220 nm
Sample 1. Ribonuclease A
2. Cytochrome C
3. Lysozyme 
(1.65 µg)
(1.65 µg)
(1.65 µg)

Applications

Performance Comparison

Chromatogram

Condition
Mobile Phase A: 20mmol/l Tris buffer(pH8.3)
B: 20mmol/l Tris buffer(pH8.3) + 0.5mol/l NaCl
B conc. 0% (0-13min)
0→100%(13-43min)
100% (43-53min)
Flow Rate 0.5 ml/min
Temperature 8 °C
Detection ÅKTAexplorer 10 S
Sample Trypsin Inhibitor from Soybean (1mg/ml)
Injection vol. 500 µl

Data courtesy of Kei WADA, Ph. D., Department of Biological Sciences, Graduate School of Science, Osaka University

Purification of His-tag Fusion Proteins

Chromatograms

Lane 1: Protein Makers for SDS-PAGE (# 09547-16)
Lane 2: His-tag protein [pET-41b(+)] in E. Coli lysate
Lane 3: Purified fraction by COSMOGEL His-Accept SF
Lane 4: Desalted fraction
Lane 5: Purified fraction by COSMOGEL IEX Type

Condition
 Column  COSMOGEL IEX Type Q 4.6mmI.D.-50mm
Mobile Phase A: 20mmol/l Tris buffer(pH8.0)
B: 20mmol/l Tris buffer(pH8.0) + 0.5mol/l NaCl
B conc. 30% (0min)
35% (0-30min)
50% (30-50min)
100% (50-55min)
30% (55min-)
Flow Rate 0.5 ml/min
Temperature Room Temperature
Detection UV280nm
Sample His-tag Protein in 20mmol/l Tris buffer(pH8.0) (Lane 4)
Injection vol. 5.0ml

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Ordering Information

Ion Exchange Mode Product Name Application Size
I.D. x Length (mm)
Product No. Price
Anion-exchange Type COSMOGEL IEX
Type Q
For Purification 4.6 x 50 06266-31 e-Nacalai
COSMOGEL IEX
Type Q-N
For Ultra-fast Analysis 4.6 x 30 06264-51
COSMOGEL IEX
Type Q-N
For Precise Analysis 4.6 x 100 06258-41
Cation-exchange Type COSMOGEL IEX
Type S
For Purification 4.6 × 50 06252-01
COSMOGEL IEX
Type S-N
For Ultra-fast Analysis 4.6 x 30 06251-11
COSMOGEL IEX
Type S-N
For Precise Analysis 4.6 × 100 06250-21
Amphoteric ion-exchange type COSMOGEL IEX
Type M
For Purification 4.6 x 50 06248-71
COSMOGEL IEX
Type M-N
For Precise Analysis 4.6 x 100 06244-11

Column Material : PEEK  Connection : Waters Type

(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze