Products

COSMOSIL CHiRAL Series

Features

  • Immobilized selectors can withstand many different solvents
  • Sharpen peaks with 3 µm particles
  • Preparative separations with 5 µm particles
  • Equivalent performance to columns currently on the market
  • Competitive price

Product Information

Conceptual Model

Conceptual Model

COSMOSIL CHiRAL Series

Packing Material COSMOSIL CHiRAL
3A, 5A
COSMOSIL CHiRAL
3B, 5B
COSMOSIL CHiRAL
3C, 5C
USP Code L99
Base Material Silica gel
Particle Size 3, 5 µm
Chiral Selector Amylose tris (3,5-dimethylphenyl
carbamate)
Cellulose tris (3,5-dimethylphenyl
carbamate)
Cellulose tris (3,5-dichlorophenyl
carbamate)
Structure Structure Structure
Chiral Selector Type Immobilized
Usable pH Range 2-9

Immobilized Stationary Phase

High Resistance to Solvents

The COSMOSIL CHiRAL series uses a chiral selector that is chemically bonded to the silica gel, which results in high resistance to solvents. Tetrahydrofuran (THF) strips coated selectors from the stationary phase, reducing theoretical plates to less than 1/5 in our experiment. However, the same selectors bonded to the silica withstood repeated injections of THF.

Application

Conditions
Column size 2.6 mm I.D. x 250 mm Sample trans-Stilbene oxide (0.5mg/ml)
Mobile phase n-Hexane / 2-Propanol = 90/10
Flow Rate 1.0 ml/min
Temperature 30°C
Detection UV270nm Inj.Vol. 10 µl

Usable Solvents

Due to its immobilized selectors, various solvents are usable with COSMOSIL CHiRAL. A wide variety of solvents increases your selectivity options.

Solvent Immobilized Coated
Normal phase Reversed phase Normal phase
n-Hexane ◯* N/A ◯*
n-Heptane N/A
Methanol ◯* ◯*
Ethanol
2-Propanol (Isopropanol)
Acetonitrile ◯* ◯*
Tetrahydrofuran (THF) ×
t-Butyl methyl ether N/A ×
Toluene N/A ×
Chloroform N/A ×
Dichloromethane
(Methylene chloride)
N/A ×
Ethyl acetate N/A ×
Water N/A N/A
Aqueous buffer N/A N/A

〇 Usable × Unusable
* Methanol / acetonitrile and n-hexane are not miscible with each other, so they should not be mixed for LC.
Elution strength: alcohols, THF >> chloroform > t-butyl methyl ether >> alkanes

Selectivity Depends on the Solvent

Column: COSMOSIL CHiRAL 3B, 4.6 mm I.D. x 250 mm
Sample: 1-Acenaphthenol

Application

Available in 3 µm Particles

Sharp Peaks

COSMOSIL CHiRAL columns are available in 3 µm particles, which yield sharper peaks than conventional 5 µm particles. Columns for preparative separations are available with 5 µm particles.

Comparison : 5µm/3 µm (1)

Application

Conditions
Column size 4.6 mm I.D. x 250 mm Sample Flavanone (0.5mg/ml)Structure
Mobile phase n-Hexane / 2-Propanol = 90/10
Flow rate 1.0 ml/min
Temperature 30°C
Detection UV 254 nm Inj.Vol. 1.0 µl
Comparison : 5µm/3 µm (2)

Application

Conditions
Column size 4.6 mm I.D. x 250 mm Sample Ketoprofen (0.3mg/ml)
Structure
Mobile phase n-Hexane / 2-Propanol / TFA = 90/10/0.1
Flow rate 1.0 ml/min
Temperature 30°C
Detection UV 254 nm Inj.Vol. 1.0 µl

Moderate Backpressure

The COSMOSIL CHiRAL 3 series (3 µm particles) puts moderately higher pressure on the system compared to conventional 5 µm particles. This improves reproducibility for low-pressure solvents, such as hexane, that may otherwise cause pump irregularities. As shown in the figure below, increased 2-propanol concentration causes higher pressure. However, even at 50% (v/v), the pressure did not exceed 12 MPa. At the lowest concentration of 5% (v/v), the backpressure was 5.3 MPa. This range allows high flexibility in mobile phase composition and selectivity.

Table

Conditions
Packing material COSMOSIL CHiRAL 3A (3µm)
COSMOSIL CHiRAL 5A (5µm)
Column size 4.6 mm I.D. x 250 mm
Mobile phase Hexane / 2-Propanol
(ratio as in figure)
Temperature 30 °C
Flow rate 1.0 ml/min

Compatible with SFC

COSMOSIL CHiRAL columns are compatible with SFC and exhibit similar performance compared to HPLC.

trans-Stilbene oxide

Condition
Column COSMOSIL CHiRAL 3A
Column Size 4.6 mm I.D. x 250 mm
Mobile Phase n-Hexane / 2-Propanol = 90/10
Flow Rate 1.0 ml/min
Temperature 30°C
Detection UV254nm
Sample trans-Stilbene oxide (2.0mg/ml)
Inj. Vol. 1.0 µl
Condition
Column COSMOSIL CHiRAL 3A
Column Size 4.6 mm I.D. x 250 mm
Mobile Phase A: CO2
B: 2-Propanol
B conc. 10%
Flow Rate 3.0 ml/min
PBR 10 MPa
Temperature 40°C
Detection UV254nm
Sample trans-Stilbene oxide (2.0mg/ml)
Inj. Vol. 2.0 µl

1-Phenoxy-2-propanol

Condition
Column COSMOSIL CHiRAL 3C
Column Size 4.6 mm I.D. x 250 mm
Mobile Phase n-Hexane / 2-Propanol = 90/10
Flow Rate 1.0 ml/min
Temperature 30°C
Detection UV270nm
Sample 1-Phenoxy-2-propanol (1.0mg/ml)
Inj. Vol. 1.5 µl
Condition
Column COSMOSIL CHiRAL 3C
Column Size 4.6 mm I.D. x 250 mm
Mobile Phase A: CO2
B: 2-Propanol
B conc. 10%
Flow Rate 3.0 ml/min
PBR 10 MPa
Temperature 40°C
Detection UV270nm
Sample 1-Phenoxy-2-propanol (1.0mg/ml)
Inj. Vol. 3.0 µl

Column Selection

COSMOSIL CHiRAL offers 3 different chiral selectors that, together, have a high probability of separating your sample. Of 28 samples in our test, 27 pairs of enantiomers were fully separated. For samples that do not separate easily with any column, please adjust the type and concentration of solvents in your mobile phase.

Sample, mobile phase COSMOSIL CHiRAL A COSMOSIL CHiRAL B COSMOSIL CHiRAL C
trans-Stillbene oxide
Structure
Hexane / 2-Propanol
= 90 / 10
Complete Separation

Doble ring

Application
Complete Separation

Application
Complete SeparationApplication
2-(2,4-Dichlorophenoxy)
propionic acid
Structure
Hexane / 2-Propanol / TFA
= 95 / 5 / 0.1
Complete Separation

Doublering

Application
Application Complete SeparationApplication
1-(1-Naphthyl)ethanol
Structure
Hexane / 2-Propanol
= 90 / 10
Application Complete Separation

Doublering

Application
Complete SeparationApplication
Nicardipine
Structure
Hexane / 2-Propanol / Diethylamine
= 90 / 10 / 0.1
Application Application Complete Separation

Doublerings

Application
Chiral selector hit rate*(n = 28) 60.70% 67.90% 71.40%

* Complete separation (hit) is defined as the two enantiomer peaks having resolution (RS) greater than or equal to 1.5.
The best separation for each sample is marked with double rings.

Applications

Neutral Compounds

Methyl DL-Mandelate

Application

Conditions
Column COSMOSIL CHiRAL 3B Sample Methyl DL-Mandelate (1.0mg/ml)
Structure
Column size 4.6 mm I.D. x 250 mm
Mobile phase n-Hexane / 2-Propanol = 90/10
Flow rate 1.0 ml/min
Temperature 30°C
Detection UV 220 nm Inj.Vol. 0.75µl

1,1’-Bi-2-naphthol

Application

Conditions
Column COSMOSIL CHiRAL 3C Sample 1,1’-Bi-2-naphthol (0.5mg/ml)
Structure
Column size 4.6 mm I.D. x 250 mm
Mobile phase n-Hexane / 2-Propanol = 90/10
Flow rate 1.0 ml/min
Temperature 30°C
Detection UV335nm Inj.Vol 1.5 µl

Acidic Compounds

To prevent tailing and lack of detection, add an appropriate acid to the mobile phase (about 0.1%).

N-Carbobenzoxy-DL-alanine

Application

Conditions
Column COSMOSIL CHiRAL 3B Sample N-Carbobenzoxy-DL-alanine (0.5mg/ml)
Structure
Column size 4.6 mm I.D. x 250 mm
Mobile phase n-Hexane / 2-Propanol / TFA = 90/10/0.1
Flow rate 1.0 ml/min
Temperature 30°C
Detection UV210nm Inj.Vol 0.75 µl

Mecoprop

Conditions
Column COSMOSIL CHiRAL 3A Sample Mecoprop (0.5mg/ml)Structure
Column size 4.6 mm I.D. x 250 mm
Mobile phase n-Hexane / 2-Propanol / TFA = 95/5/0.1
Flow rate 1.0 ml/min
Temperature 30°C
Detection UV230nm Inj.Vol 0.5 µl

Basic Compounds

To prevent tailing and lack of detection, add an appropriate base to the mobile phase (about 0.1%).

Carbinoxamine

Application

Conditions
Column COSMOSIL CHiRAL 3A Sample Carbinoxamine (1.0 mg/ml)
Structure
Column size 4.6 mm I.D. x 250 mm
Mobile phase n-Hexane / 2-Propanol / Diethylamine
= 90/10/0.1
Flow rate 1.0 ml/min
Temperature 30°C
Detection UV225nm Inj.Vol 0.5 µl

Tramadol

Application

Conditions
Column COSMOSIL CHiRAL 3C Sample Tramadol (1.0mg/ml)Structure
Column size 4.6 mm I.D. x 250 mm
Mobile phase n-Hexane / 2-Propanol / Diethylamine = 95/5/0.1
Flow rate 1.0 ml/min
Temperature 30°C
Detection UV225nm Inj.Vol 1.0 µl

icon See more COSMOSIL Applications

Downloads

Brochures

Ordering Information

Analytical Columns (Particle Size: 3 µm)

COSMOSIL CHiRAL 3 Packed Columns

Product Name Column Size
(mmI.D.xmm)
Product No. Inquiry
COSMOSIL CHiRAL 3A 2.0 x 150 15778-51 Inquire
Now
4.6 x 250 15779-41
COSMOSIL CHiRAL 3B 2.0 x 150 15783-71 Inquire
Now
4.6 x 250 15784-61
COSMOSIL CHiRAL 3C 2.0 x 150 15788-21 Inquire
Now
4.6 x 250 15789-11

Analytical / Preparative Columns (Particle Size: 5 µm)

COSMOSIL CHiRAL 5 Packed Columns

Product Name Column Size
(mmI.D.xmm)
Product No. Inquiry
COSMOSIL CHiRAL 5A 4.6 x 250 15780-01 Inquire
Now
10.0 x 250 15781-91
20.0 x 250 15782-81
COSMOSIL CHiRAL 5B 4.6 x 250 15785-51 Inquire
Now
10.0 x 250 15786-41
20.0 x 250 15787-31
COSMOSIL CHiRAL 5C 4.6 x 250 15790-71 Inquire
Now
10.0 x 250 15791-61
20.0 x 250 15792-51

* For 10 mm and 20 mm I.D. columns, please inquire about delivery time.

(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze