Products

COSMOSIL NH2-MS

Features

Column Image

  • Aminopropyl bonded stationary phase
  • Different selectivity with Sugar-D

Product Information

Column Specifications

Column Specifications

Comparison of Adsorption

NH2-MS offer better separation than Sugar-D in some cases depending on samples.

Condition
Column Size 4.6 mm I.D . x 250 mm Sample 1. Sucrose 10 µg
Flow Rate 1 ml/min 2. Maltose 10 µg
Temperature 30°C 3. Lactose 10 µg
Detection RI 4. D-(+)-Trehalose 10 µg

Applications

See more COSMOSIL Applications

Usage information

Column 5 µm NH2
I.D. (mm) 2.0 3.0 4.6 10.0 20.0
Washing method 1. Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. For example, if your mobile phase was 50:50 acetonitrile/20 mmol/l phosphate buffer, wash with 50:50 acetonitrile/water.

2. Remove adsorbed compounds and fix unstable baselines: Wash with 50:50 acetonitrile/water.
Storage conditions Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid.
Then, replace the solvent with 90:10 acetonitrile/water.

Tightly plug the column, and store in a cool and dry place.
Usable pH range pH2~pH7.5
Max. pressure 20MPa 15MPa
Temperature range The maximum usable temperature is 60°C. However. for regular use, please use at a constant temperature between 20°C and 50°C.
Usable solvents - Acetonitrile/water mobile phases are recommended.
Other instructions - Buffer concentration is usually sufficient at 0.005 - 0.02 mol/L.
- Always filter mobile phases using a 0.5 µm or finer filter before use.
- Methanol is not recommended as a mobile phase.
- If using a buffer or acid, we recommend using the column only for that mobile phase. Buffers may affect performance even after washing the column.
- Neutral pH will maximize retention time.

Downloads

Brochures

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References

Reference list

Ordering Information

Analytical and Preparative Column (Particle size : 5 µm)

COSMOSIL NH2-MS

Product Name Size Product No. Price
COSMOSIL NH2-MS Guard Column
4.6 mm l.D x 10 mm 38241-51 e-Nacalai
10 mm l.D x 20 mm 38242-41
20 mm l.D x 50 mm 06093-91
COSMOSIL NH2-MS Guard Cartridge
Guard cartridge holder is required.
4.6 mm l.D. x 10 mm 19182-34 e-Nacalai
COSMOSIL NH2-MS Packed Column
4.6 mm l.D x 150 mm 38245-11 e-Nacalai
4.6 mm l.D x 250 mm 38246-01
10 mm l.D x 250 mm 38249-71
20 mm l.D x 250 mm 38250-31

(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze