Products

COSMOSIL SFC Columns

Column Image

Supercritical Fluid Chromatography (SFC) has become more attractive because it offers some advantages over HPLC, such as high speed, unique selectivity and environmentally friendly separations. Many conventional normal-phase stationary phases, such as diol, amino and cyano, have been used for SFC applications. However, these phases present limitations for separations. COSMOSIL SFC Columns have been developed to enhance the capability of SFC separations.

Poster from SFC Asia 2017: Research into the Retention Mechanism of SFC pdf (358 KB)

Product Information

COSMOSIL SFC Columns

Nacalai Tesque has developed columns specially designed for SFC in collaboration with Nacalai USA and Pfizer, Inc. Global R&D: COSMOSIL HP, PY (equivalent to 2-ethylpyridine) and Quinoline. In addition to these, our HPLC columns Cholester and PBr have been tested for use with SFC.

Packing Material HP PY Quinoline Cholester PBr
Particle Sizes 3, 5 µm 2.5 µm 5 µm 2.5 µm 5 µm 5 µm
Pore Sizes 120 Å 130 Å 120 Å 130 Å 120 Å 120 Å
Surface Area 300 m2/g 330 m2/g 300 m2/g 330 m2/g 300 m2/g 300 m2/g
Structure Stationary Phase Stationary Phase Stationary Phase Stationary Phase Stationary Phase
Stationary
Phase
3-Hydroxyphenyl
group
Pyridinyl group Quinoline group Cholesteryl group Pentabromobenzyl group

Pharmaceutical Analysis

Each phase has different retention properties.

Imipramine

Conditions
Column size 2.1 mm I.D. x 150 mm Sample Imipramine (1mmol/l)

Mobile phase A: CO2
B: 0.1% CH3COONH4 -Methanol
B conc. 0→60% (0→14min), 60% (14-17min)
Flow Rate 0.8 ml/min
BPR 10 MPa
Temperature 40°C
Detection UV260nm Inj.Vol. 2.0 µl

Data courtesy of Kyushu University Medical Institute of Bioregulation Research Center for Transomics Medicine Division of Metabolomics

Procaine

Conditions
Column size 2.1 mm I.D. x 150 mm Sample Procaine (1mmol/l)


Mobile phase A: CO2
B: 0.1% CH3COONH4 - Methanol
B conc. 0→60% (0→14min), 60% (14-17min)
Flow Rate 0.8 ml/min
BPR 10 MPa
Temperature 40°C
Detection UV280nm Inj.Vol. 2.0 µl

Data courtesy of Kyushu University Medical Institute of Bioregulation Research Center for Transomics Medicine Division of Metabolomics

Comparison of Retention Behavior

The following three stationary phases were evaluated for their retention of hydrophilic, hydrophobic, acidic and basic compounds. COSMOSIL HP and PY elute hydrophobic compounds first and retain hydrophilic compounds longer, whereas PBr elutes in the reverse order, exhibiting high retention for hydrophobic compounds. HP had the longest retention for basic compounds.

Applications

Conditions
Column size 4.6 mm I.D. x 150 mm Flow Rate 0.8 ml/min
Mobile phase A: CO2
B: 0.1% CH3COONH4 - Methanol
B conc. 0→50% (0→14min), 50% (14-17min)
BPR 10 MPa
Temperature 40°C
Detection ESI-MS/MS, MRM
Sample
  1. Uridine
  2. 3’ ,5’ -Dibenzyloxyacetophenone
  3. 4-Ethylbenzenesulfonic Acid
  4. Benzyltrimethylammonium
Sample conc. 8 µmol/l
Inj.Vol. 2.0 µl

Data courtesy of Kyushu University Medical Institute of Bioregulation Research Center for Transomics Medicine Division of Metabolomics

Applications

COSMOSIL HP(3-Hydroxyphenyl)

Non-steroidal anti-inflammatory drugs

Applications

Conditions
Column size 4.6 mm I.D. x 150 mm Sample
  1. Ibuprofen
  2. Fenoprofen
  3. Flurbiprofen
  4. Ketoprofen
  5. Indoprofen
Mobile phase Carbon Dioxide : Methanol
Gradient 95:5 to 65:35 over 6 minutes
Flow rate 5.0 ml/min
Temperature 30°C
Detection UV 230 nm

Beta Blockers
Peak elution order reversal under identical Conditionss

Applications

Conditions
Column size 4.6 mm I.D. x 150 mm Sample
  1. Pindolol
  2. Atenolol
Mobile phase Carbon Dioxide : Methanol
w/20 mM ammonium formate
Gradient 95:5 to 50:50 over 4 minutes
Flow rate 5.0 ml/min
Temperature 30°C
Detection APCI(+)

COSMOSIL PY (Pyridinyl)

Hydrophilic organics

Applications

Conditions
Column size 4.6 mm I.D. x 150 mm
Flow rate 5.6 mL/min
Pressure 140 bar
Temperature Ambient
Mobile phase Methanol gradient 5-50%
@18%/minute;
hold @ 50% 0.1 min;
return to 5% @99%/min

COSMOSIL Quinoline

The structural similarities between polar lipids, such as cholesterol and related analogs, have posed chromatographic and spectrometric challenges to analysts interested in quantifying these potential biomarkers. COSMOSIL Quinoline has been developed to improve the separation of these structural isomers utilizing the π-π interactions and structural rigidity of the naphthylethyl phase and the hydrogen bonding of the pyridine phase.

Steroids

Applications

Conditions
Column size 4.6 mm I.D. x 150 mm Sample Applications
Mobile phase Carbon Dioxide (A)
: Methanol (B)
Gradient 2% - 7% B in 4 minutes
Flow rate 4.5 ml/min
Temperature 30°C
Detection APCI (+)

Caffeine analogs

Applications

Conditions
Column size 4.6 mm I.D. x 150 mm Sample
Mobile phase Carbon Dioxide:Methanol = 95:5
Flow rate 4.5 ml/min
Pressure 160 bar
Temperature 30°C
Detection UV 260 nm

COSMOSIL Cholester

Fat-Soluble Vitamin Analysis

When used with SFC, COSMOSIL Cholester can separate fat-soluble vitamins and their impurities.

Conditions
Column size 4.6 mm I.D. x 250 mm
Mobile phase IPA in CO2
IPA conc. 0→10%(0→10min),10%(10→12min)
Flow rate 3.0 ml/min
BPR 15 MPa
Temperature 40°C
Detection UV-VIS
Sample conc 2.0 mg/ml
Inj. Vol 1.0 µl
Sample

COSMOSIL Cholester exhibits strong retention for fat-soluble vitamins and is suitable for on-line SFE-SFC using Shimadzu’s Nexera UC. The on-line extraction from food also produced triglyceride impurities, which were successfully separated from the
vitamins.
Data courtesy of Shimadzu Corporation

COSMOSIL SFC Columns

Amitriptyline

Conditions
Column size 2.1 mm I.D. x 150 mm Temperature 40°C
Mobile phase A: CO2
B: 0.1% CH3COONH4 -Methanol
B conc. 0→60% (0→14min), 60% (14-17min)
Detection UV250nm
Inj.Vol.: 2.0 µl
Sample Amitriptyline (1 mmol/l)
Flow rate 0.8 ml/min
BPR 10 MPa

Data courtesy of Kyushu University Medical Institute of Bioregulation Research Center for Transomics Medicine Division of Metabolomics

Reserpine

Conditions
Column size 2.1 mm I.D. x 150 mm BPR 10 MPa
Mobile phase A: CO2
B: 0.1% CH3COONH4 -Methanol
B conc. 0→60% (0→14min), 60% (14-17min)
Temperature 40°C
Detection UV 260nm
Inj.Vol.: 2.0 µl
Flow rate 0.8 ml/min
Sample Reserpine (1mmol/l)

Data courtesy of Kyushu University Medical Institute of Bioregulation Research Center for Transomics Medicine Division of Metabolomics

3-Aminobenzoic Acid

Conditions
Column size 2.1 mm I.D. x 150 mm BPR 10 MPa
Mobile phase A: CO2
B: 0.1% CH3COONH4 -Methanol
B conc. 0→60% (0→14min), 60% (14-17min)
Temperature 40°C
Detection UV 250nm
Inj.Vol.: 2.0 µl
Flow rate 0.8 ml/min
Sample 3-Aminobenzoic Acid (10mmol/l)

Data courtesy of Kyushu University Medical Institute of Bioregulation Research Center for Transomics Medicine Division of Metabolomics

Benzoic Acid

Conditions
Column size 2.1 mm I.D. x 150 mm BPR 10 MPa
Mobile phase A: CO2
B: 0.1% CH3COONH4 -Methanol
B conc. 0→60% (0→14min), 60% (14-17min)
Temperature 40°C
Detection UV 230nm
Inj.Vol.: 2.0 µl
Flow rate 0.8 ml/min
Sample Benzoic Acid (10mmol/l)

Data courtesy of Kyushu University Medical Institute of Bioregulation Research Center for Transomics Medicine Division of Metabolomics

See more COSMOSIL Applications

Downloads

Brochures

Technical Guide

  • COSMOSIL SFC Technical Guidepdf (1 MB)

Posters

  • Research into the Retention Mechanism of SFC
    (Poster from SFC Asia 2017, Osaka, Japan)
    pdf (358 KB)
  • Development of novel stationary phases for Supercritical Fluid Chromatography (SFC) (Poster from HPLC 2011, Budapest, Hungary)
    pdf (242 KB)

Ordering Information

Analytical and Preparative Column (Particle size : 5 µm)

COSMOSIL HP (3-Hydroxyphenyl)

Product Name Column Size
(mmI.D.xmm)
Product No. Inquiry
Packed Column
(5 µm)
2.0 x 150 13787-91 Inquire
Now
4.6 x 250 13788-81
10.0 x 250 13789-71
20.0 x 250 13790-31
Guard Column
(5 µm)
10.0 x 20 13791-21 Inquire
Now
Packed Column
(3 µm)
2.0 x 150 13792-11 Inquire
Now
4.6 x 250 13793-01

COSMOSIL PY (Pyridinyl)

Product Name Column Size
(mmI.D.xmm)
Product No. Inquiry
Packed Column
(5 µm)
2.0 x 150 13818-81 Inquire
Now
4.6 x 250 13827-61
10.0 x 250 13828-51
20.0 x 250 13829-41
Guard Column
(5 µm)
10.0 x 20 13830-01 Inquire
Now
Packed Column
(3 µm)
2.0 x 150 13831-91 Inquire
Now
4.6 x 250 13832-81

COSMOSIL Quinoline

Product Name Column Size
(mmI.D.xmm)
Product No. Inquiry
Packed Column
(5 µm)
2.0 x 150 Inquire Inquire
Now
4.6 x 100 Inquire
4.6 x 150 Inquire
10.0 x 150 Inquire
20.0 x 150 Inquire
Packed Column
(2.5 µm)
3.0 x 50 Inquire Inquire
Now
3.0 x 100 Inquire
3.0 x 150 Inquire

COSMOSIL Cholester

Product Name Column Size
(mmI.D.xmm)
Product No. Inquiry
Packed Column
(5 µm)
4.6 x 150 05976-61 Inquire
Now
4.6 x 250 05977-51
10.0 x 250 05979-31
20.0 x 250 05982-71

COSMOSIL PBr

Product Name Column Size
(mmI.D.xmm)
Product No. Inquiry
Packed Column
(5 µm)
4.6 x 150 12394-61 Inquire
Now
4.6 x 250 12395-51
10.0 x 250 12397-31
20.0 x 250 12398-21

(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze