Column Specifications

Column Specifications

Features

About Sugar-D

Development of Sugar-D

Aminopropyl columns are commonly used for analysis of monosaccharides and oligosaccharides; however, this type of column has several problems, including low durability, poor separation, and adsorption and peak tailing with some analytes. Carbamoylbased columns separate anomers, which may not be desirable. To solve these problems, we developed a specialty column for sugar analysis with high durability and performance that does not induce irreversible adsorption.

Different selectivity from aminopropyl columns

COSMOSIL Sugar-D has a polyamine-based stationary phase, which results in different selectivity from primary anime-based phases, such as aminopropyl.

Comparison to aminopropyl columns

• Sucrose and Turanose

Condition
Column Size	4.6 mm I.D. x 250 mm
Flow Rate	1 ml/min
Temperature	30°C
Detection	RI
Sample	1; Fructose (26.5mg/ml) 2; Glucose (21.2mg/ml) 3; Sucrose (5.3mg/ml) 4; Turanose (5.9mg/ml) 5: Maltose (5.3mg/ml)
Inj.Vol.	17‹µl

Superior quantitative results

Aldehyde groups in the open-chain form of sugars can form Schiff bases with the amino groups on aminopropyl columns, causing low recovery and peak tailing. COSMOSIL Sugar-D was designed to avoid this, so sugars like arabinose and galactose, which are problematic on aminopropyl columns, elute with sharp peaks. Sugar-D is especially useful for samples of low concentration.

Quantitation at low concentration

Superior durability

The stationary phase of COSMOSIL Sugar-D is not easily hydrolyzed, so even using water as the mobile phase does not affect it much.

Change in retention factor

Eluent Condition
Eluent	Water
Flow Rate	1 ml/min
Temperature	RT

Test Condition
Column Size	4.6 mm I.D. x 250 mm
Mobile Phase	Acetonitrile : Water = 70 : 30
Flow Rate	1.0 ml/min
Temperature	30°C
Detection	RI
Sample	Maltose

Anomers remain unseparated

When analyzing sugars with amide columns, anomers may separate unless harsh conditions, such as high temperature and basic solvents, are employed. COSMOSIL Sugar-D does not separate anomers, even under mild conditions.

Comparison to amide column

Test Condition
Column Size	4.6 mm I.D. x 250 mm
Mobile Phase	(Sugar-D) Acetonitrile / H2O = 80/20 (Company E) Acetonitrile / H2O = 75/25
Flow Rate	1.0 ml/min
Temperature	30°C
Detection	RI
Sample	1; Rhamnose (10‹µg) 2; Xylose (10‹µg) 3; Fructose (10‹µg) 4; Mannose (10‹µg) 5; Glucose (10‹µg)

Analysis of Carbohydrate Mixtures by COSMOSIL Sugar-D

We have compiled a list of our Sugar-D HPLC application data in an easy-to-read format. Please click the compound name to view its data.

Coumpound	CAS No.	No. of applications
D-(+)-Glucose	50-99-7	11
D-(-)-Fructose	57-48-7	9
Xylitol	87-99-0	4
D-Psicose	551-68-8	3
Sucrose	57-50-1	7
Maltose	6363-53-7	5
D-(+)-Raffinose	17629-30-0	2
D-(+)-Mannose	3458-28-4	2
L-(+)-Rhamnose	10030-85-0	2
D-(+)-Trehalose	6138-23-4	1
D-(+)-Allose	2595-97-3	1
D-Glucitol	50-70-4	3
Maltitol	585-88-6	3
meso-Erythritol [meso-Erythrite]	149-32-6	3
myo-Inositol	87-89-8	2
Cyclodextrins	10016-20-3 7585-39-9 17465-86-0	1
D-Glucuronic Acid	6556-12-3	1
Glycerol	56-81-5	1
1-Kestose	470-69-9	1
Lactose	5989-81-1	1
Maltotriitol	32860-62-1	2
Maltotriose	1109-28-0	2
Maltotetraose	34612-38-9	1
Maltopentaose	1668-09-3	1
Maltohexaose	34620-77-4	1
Maltoheptaose	34620-78-5	1
D-(-)-Mannitol	69-65-8	1
D-Mannosamine	5505-63-5	1
Nystose	13133-07-8	1
Palatinit	64519-82-0	1
Palatinose	13718-94-0	1
D-(+)-Xylose	58-86-6	1
1-Fructofuranosyl-D-nystose		1
Fructooligosaccharides		1
Isomaltooligosaccharides		1
Maltotertaitol		1
Xylooligosaccharides		1

Sugars in Sports Drinks

Condition
Column Size	(4.6 mm I.D. x 250 mm) x2
Mobile Phase	Acetonitrile/ H2O = 75/25
Flow Rate	1.0 ml/min
Temperature	50°C
Detection	RI
Sample	1; meso-Erythritol (meso-Erythrite) (10‰µg) 2; D-(-)-Fructose (10‰µ‰g) 3; D-Glucitol (10‰µ‰g) 4; D-(+)-Glucose (10‰‰µg) 5; Sucrose (10‰µ‰g) 6; Maltose (10‰µ‰g) 7; Maltotriitol (10‰‰µg) 8; Maltotriose (10‰‰µg)

Honey

Condition
Column Size	4.6 mm I.D. x 250 mm
Mobile Phase	Acetonitrile/ H2O = 75/25
Flow Rate	1.0 ml/min
Temperature	30°C
Detection	RI
Sample	Honey (50mg/ml) 1; D-(-)-Fructose 2; D-(+)-Glucose
Inj.Vol.:	5.0µl

Sugar Alcohols

Condition
Column Size	4.6 mm I.D. x 250 mm
Mobile Phase	Acetonitrile/ H2O = 75/25
Flow Rate	1.0 ml/min
Temperature	30°C
Detection	RI
Sample	1; Glycerol (10µg) 2; meso-Erythritol (meso-Erythrite) (10µg) 3; Xylitol (10µg) 4; D-Glucitol (10µg) 5; Maltitol (10µg) 6; myo-Inositol (10µg)

Maltooligosaccharides

Condition
Column Size	4.6 mm I.D. x 250 mm
Mobile Phase	Acetonitrile/ H2O = 65/35
Flow Rate	1.0 ml/min
Temperature	30°C
Detection	RI
Sample	1; D-(+)-Glucose (10‰µg) 2; Maltose (10‰µg) 3; Maltotriose (10‰µg) 4; Maltotetraose (10‰µg) 5; Maltopentaose (10‰µg) 6; Maltohexaose (10‰µg) 7; Maltoheptaose (10‰µg)

See more COSMOSIL Applications

Column	5 µm Sugar-D
I.D. (mm)	2.0	3.0	4.6	10.0	20.0
Washing method	Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. For example, if your mobile phase was 50:50 acetonitrile/20 mmol/l phosphate buffer, wash with 50:50 acetonitrile/water. Remove adsorbed compounds and fix unstable baselines: Wash with 50:50 acetonitrile/water or up to 100% water.
Storage conditions	Wash the column to remove buffer, salts and/or acid. Then, replace the solvent with 90:10 acetonitrile/water. Tightly plug the column, and store in a cool and dry place.
Usable pH range	pH 2 - 7.5
Max. pressure	20MPa			15MPa
Temperature range	The maximum usable temperature is 60°C. However. for regular use, please use at a constant temperature between 20°C and 50°C.
Usable solvents	Acetonitrile/water mobile phases are recommended.
Other instructions	Buffer concentration is usually sufficient at 0.005 - 0.02 mol/L. Always filter mobile phases using a 0.5 µm filter before use. Methanol is not recommended as a mobile phase. If using a buffer or acid, we recommend using the column only for that mobile phase. Buffers may affect performance even after washing the column. Neutral pH will maximize retention time.

Inseparable Compounds

Some sugars are not separated well by Sugar-D. For these compounds, you can use COSMOSIL NH₂-MS.

Compound pair		NH2 application
Glucose	Galactose	AP-0137
Lactose	Maltose	AP-0138

You can search for others in our application search. The Sugar-D chromatogram index (applications beginning "K-") can help you determine analysis conditions.

Brochures

• COSMOSIL Sugar-D (6.3 MB)
• Acarbose Analysis (1 MB)
• Analysis of Sugars in Honey and Syrup (1.7 MB)

Reference list

• COSMOSIL Sugar-D reference list