LIQUID CHROMATOGRAPHY RELATED PRODUCTS

COSMO(+)Capillary

COSMO(+)Capillary is cationic polymer coated capillary column, for electroosmotic flow (EOF) is from the chthode (-) to the direction of the anode (+) because of the cationic polymer coating inside of the capillary column.

  • Opposit EOF direction to non-coated capillary
  • High reproducibility
  • Applicable wide pH range (pH2-10)

Comparison of COSMO(+)Capillary and Non-coated Capillary

Cationic Polymer Coated Capillary Column

Electropherograms for E.coli Metabolic Extraction

The figure shows the analysis data of various kinds of metabolites. COSMO(+)Capillary can analyze them within 20 minutes by electroosmotic flow (EOF) reversal and a sheath liquid consisting of 50 mM ammonium acetate, pH8.5.
E.coli Metabolic Extraction Peak Identification:
1: Glycolate
2: Lactate
3: Fumarate
4: 2-Oxoisopentanoate
5: Succinate
6: Malate
7: PEP
8: DHAP
9: Glycerol3P
10: 3PG
11: MES(is)
12: Gluconate
13: Ribulose5P
14: G1P
15: G6P+F6P
16: 2,3DPG
17: Sedoheptulose7P
18, F1,6DP

Data courtesy of Prof. Tomoyoshi Soga, Institute for Advanced Biosciences (IAB), Keio University
Experimental Conditions:
Sample:
Capillary:
Electrolyte:
Applied Potential:
Injection:
Temperature:
Sheath liquid:		 E.coli W3110, 109 Cells
COSMO(+) 50 µm I.D. x 110 cm
50 mM Ammonium Acetate,  pH8.5
-30 kV
30 sec at 50 mbar
20°C
 10 µl/min 5 mM Ammonium Acetate in 50% (v/v) Methanol - Water

Application Data

The figure shows high reproducibility when COSMO(+)Capillary is used repetitively (26 reference standards).
Reproducibility of COSMO(+)Capillary
Data courtesy of Prof. Tomoyoshi Soga, Institute for Advanced Biosciences (IAB), Keio University
Experimental Conditions:
Sample Concentration
Capillary
Electrolyte
Applied Potential
Injection
Temperature
Sheath liquid		 50 µmol/l each
COSMO(+) 50 µm I.D. x 110 cm
50 mM Ammonium Acetate, pH8.5
-30 kV
30 sec at 50 mbar
20°C
10 µl/min 5 mM Ammonium Acetatein 50% (v/v) Methanol - Water
The COSMO(+)Capillary enables separation of Succinic Acid which fused silica can not separate. Furthermore it has shorter analysis time for other acids than fused silica.
Separation of Succinic Acid (a) Lactic Acid (m/z=89)
(b) Succinic Acid (m/z=117)
(c) Malic Acid (m/z=133)
(d) Uric Acid (m/z=167)
(e) Citrate (m/z=191)
Data courtesy of Yoshihide Tanaka, National Institute of Advanced Industrial Science and Technology
Experimental Conditions:
Capillary
Electrolyte
Applied Potential
Injection
Temperature
Sheath liquid		 COSMO(+) 50 µm I.D. x 110 cm
50 mM ammonium acetate, pH8.5
-30 kV
-10 kV × 30 sec
25°C
10 mM Ammonium Carbonate: 2-Propanol =1:1

Ordering information

Product name PKG size Product No.
COSMO(+)Capillary (50 µm I.D. x 120 cm)
2 PKG 07584-44

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