Reagents
Epitope Tag Antibody
Nacalai Tesque carries an impressive variety of antibodies. Apart from the wide range of secondary antibodies, recently tag antibodies are developed in increased numbers. Presently available tagged antibody types are: GFP, c-Myc, His, V5, GST. Most of these types are available in POD coupled form as well. Uniquely, GFP (Rat IgG2a) is suitable for immunohistochemical detection!
- Anti-GFP(Rat lgG2a), Monoclonal(GF090R)
- Anti-c-Myc(mouse lgG1-κ), Monoclonal
- Anti-GFP(mouse lgG1-κ), Monoclonal(GF200)
- Anti-GST(mouse lgG2a-κ), Monoclonal(GS019)
- Anti-V5(mouse lgG1-κ), Monoclonal(V5005)
- Anti-6xHis(Mouse lgG1-κ), Monoclonal(HI192)
- Ordering Infirmation
Anti-GFP(Rat lgG2a), Monoclonal(GF090R)
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Feature
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| Clone | GF090R | |
| GF090R | IgG2a(Rat) | |
| Product form | Liquid | |
| Immunogen | Liquid | |
| Application | Immunohistochemstry 1:1000 - 1:2000 Western blotting 1:1000 - 1:2000 ELISA 1:2000 - 1:20000 |
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Immunocytochemistry
| Sample | Mouse brain (nerve cell) |
| Primary antibody | Anti-GFP(Rat IgG2a), Monoclonal (1:1000) RT, O/N |
| Secondary antibody | Anti-Rat IgG-Cy3 (1:300) RT, 1hr |
| Blocking | 5% normal goat serum / 0.2% TritonX-100 in PBS |
| Fixing method | 4% Paraformaldehyde |
Data courtesy of Dr.Yoshihara Y., RIKEN Brain Science Institute
Western blotting
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| Data courtesy of Dr.Yoshida K., Department of Molecular Genetics,Medical Research Institute, Tokyo Medical and Dental University | ||||||||||
Reference
- Nakamura, M. et al. Molecular Vision 16, 425-437(2010)
- Nishide, K. et al. PLoS ONE 4(8), e6869(2009)
- Nagao, M. et al. The Journal of Cell Biology 183(7), 1243-1257(2008)
- Ono, K. et al. Development Biology 320(2), 356-468(2008)
- Nakashiba T. et al. Science 319(5867), 1260-1264 (2008)
- Batista-Brito R et al. The Journal of Neuroscience 28(15), 3966-3975 (2008)
- Esumi S. et al. Neuroscience Research 60(4), 439-451 (2008)
- Fogarty M. et al. The Journal of Neuroscience 27(41), 10935-10946 (2007)
- Sasamura T. et al. Development 134, 1347-1356 (2007)
- Sato Y. et al. The Journal of Neuroscience 27(7), 1606-1615 (2007)
- Yamada M. et al. Stem Cells 25(3), 562-570 (2007)
- Togashi H. et al. The Journal of Cell Biology 174, 141-151 (2006)
- Ogata M. et al. Molecular and Cell Biology 26(24), 9220-9231 (2006)
- Sato Y. et al. The Journal of Neuroscience 25(20), 4889-4897 (2005)
Anti-c-Myc(mouse lgG1-κ), Monoclonal
| Clone | MC045 |
| Isotype | IgG1-κ(mouse) |
| Product form | Liquid |
| Immunogen | c-Myc synthetic peptide [EQKLISEEDL] conjugated with KLH |
| Application | Western blotting 1:1000 - 1:2000 Immunoprecipitation 1:400 - 1:1000 Immunocytochemistry 1:400 - 1:1000 ELISA 1:2000 - 1:20000 |
Immunocytochemistry
| Sample | SH-SY5Y cell in which c-Myc-tagged TCP1β is expressed |
| Primary antibody | Anti-c-Myc (Mouse IgG1-κ) (2.5 μg/ml) Anti-Protein X |
| Secondary antibody | Alexa Fluor 546-conjugated antibody (1:400) Alexa Fluor 488-conjugated antibody (1:400) |
| Data courtesy of RIKEN Brain Science Institute | |
| Sample | HeLa cell in which c-Myc-tagged(N-terminal) hnRNP A1 is expressed |
| Primary antibody | Anti-c-Myc (Mouse IgG1-κ) (1:1000) |
| Secondary antibody | FITC-conjugated goat affinity purified f(ab')2 fragment to mouse IgG (whole molecule) (1:200) |
| Fixation method | 2% Formaldehyde in PBS (RT, 30min) |
| Permeabilization | Acetone (-20°C, 3min) |
| Blocking | 3% BSA in PBS (RT) |
| Data courtesy of Dr. Kataoka N., Department of Genetics and Molecular Biology, Laboratry of Biochemistry, Institute for Virus Research, Kyoto University. |
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Anti-GFP(mouse lgG1-κ), Monoclonal(GF200)
| Clone | GF200 |
| Isotype | IgG1-κ(Mouse) |
| Product form | Liquid |
| Immunogen | His-GFP (full length) fusion protein |
| Application | Western blotting 1:1000 - 1:2000 ELISA 1:2000 - 1:20000 |
Western blotting
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| Sample | lane 1) HeLa:GFP-H2A-Bbd lane 2) HeLa:GFP-H2B lane 3) HeLa |
| Data courtesy of Dr. Kimura H., Horizontal Medical Research Organization, Kyoto University Faculty of Medicine |
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Western blotting
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| Sample | Protein extract from seedlings of Arabidopsis (10mg) Lane1 : Non-transformants (Control) Lane2 : Transformants |
| Primary antibody | Anti-GFP(mouse IgG-κ) (1:5000) |
| Secondary antibody | Anti-mouse IgG-HRP (1:2000) |
| Data courtesy of Dr. Shimada T., Department of Botany, Graduate School of Science, Kyoto University | |
Anti-GST(mouse lgG2a-κ), Monoclonal(GS019)
| Clone | GS019 |
| Isotype | IgG2a-κ(mouse) |
| Product form | Liquid |
| Immunogen | Glutathione - S - Transferase(GST) |
| Application | Western blotting 1:1000 - 1:2000 Immunoprecipitation 1:400 - 1:1000 ELISA 1:2000 - 1:20000 |
Western blotting
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| Gel | 12.5% SDS-PAGE |
| Blocking | Blocking One (Nacalai Tesque) / 1 hr at RT |
| Primary antibody | anti-GST (Mouse IgG2a-κ),(GS019) (1:2000 with Blocking One) / 1 hr at RT |
| Secondary antibody | anti-mouse Ig-HRP(1:1000 with TBS-T) / 30 min at RT |
| Wash | TBS-T (5 min x 3) |
| Detection | Luminol Reagent (Santa Cruz) |
| Sample | lane 1: Lysate from E.coli expressioning GST protein lane 2: Glutathione Sepharose flow-through fraction lane 3: Glutathione Sepharose elution fraction |
| Data courtesy of Dr. Shimizu Y., The Institute of Medical Science, The University of Tokyo |
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Anti-V5(mouse lgG1-κ), Monoclonal(V5005)
| Clone | V5005 |
| Isotype | IgG1-κ(mouse) |
| Product form | Liquid |
| Immunogen | V5 synthetic peptide [GKPIPNPLLGLDST] conjugated with KLH |
| Application | Western blotting 1:1000 - 1:2000 Immunoprecipitation 1:400 - 1:1000 ELISA 1:2000 - 1:20000 |
Western blotting, Immunoprecipitation
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| (A) Western blotting VE-Card.-V5 | |
| Sample | Lysate from 293T cell in which V5-tagged (C-terminal) pCDNA dest40-VE-cadherin is expressed |
| Antibody | Anti-V5 (Mouse IgG1-κ) (1:5000) |
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| (B) Immunoprecipitation VE-Card. -V5 | |
| Sample | Lysate (A) |
| Antibody | Anti-V5 (Mouse IgG1-κ) (1μl) |
| Western blotting | Anti-V5 (Mouse IgG1-κ) (1:2000) |
Anti-6xHis(Mouse lgG1-κ), Monoclonal(HI192)
| Clone | HI192 |
| Isotype | IgG1-κ(mouse) |
| Product form | Liquid |
| Immunogen | 6xHis synthetic peptide [HHHHHH] conjugated with KLH |
| Application | Western blotting 1:1000 - 1:2000 ELISA 1:2000 - 1:20000 |
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| Filter | FluoroTrans[PALL] |
| Blocking | 5% non-fat dry milk/PBS (30 min) |
| Primary antibody | anti-6xHis (Mouse IgG1-κ),Monoclonal (HI192) |
| Wash | .25% Tween-20/TBS (10 min x 3) |
| Secondary antibody | anti-mouse IgG-HRP(1:1000) / 2% BSA / 0.25% Tween-20/TBS (30 min) |
| Wash | 0.25% Tween-20/TBS (10 min x 5) |
| Detection | Luminol Reagent (Santa Cruz) |
| Sample | lysate from Sf 9 cells in which His-tagged Drosophila PTEN is expressed |
| Data courtesy of Dr. Maehama A., The Tokyo Metropolitan Institute of Medical Science (RINSHOKEN) |
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<< Ordering Information Tag Antibodies >>
| Product name | Grade | Storage | Code No. | Quantily |
|---|---|---|---|---|
| Anti-GFP (Rat IgG2a), Monoclonal (GF090R) |
BC | R | 04404-26 | 50μg |
| 04404-84 | 200μg | |||
| Anti-c-Myc (Mouse IgG1-κ), Monoclonal (MC045) | BC | R | 04362-76 | 50μg |
| 04362-34 | 200μg | |||
| Anti-GFP (Mouse IgG1-κ), Monoclonal (GF200) | BC | R | 04363-66 | 50μg |
| 04363-24 | 200μg | |||
| Anti-GST (Mouse IgG2a-κ), Monoclonal (GS019) | BC | R | 04435-84 | 50μg |
| 04435-26 | 200μg | |||
| Anti-V5 (Mouse IgG1-κ), Monoclonal (V5005) | BC | R | 04434-94 | 50μg |
| 04434-36 | 200μg | |||
| Anti-6xHis (Mouse IgG1-κ), Monoclonal (HI192) | BC | R | 04428-26 | 50μg |
| 04428-84 | 200μg |
< POD Conjugated Tag Antibodies >
| Product name | Grade | Storage | Code No. | Quantily |
|---|---|---|---|---|
| Anti-c-Myc (Mouse IgG1-κ),Mono (MC045),POD Conjugated | BC | R | 04554-24 | 50μg |
| Anti-GFP (Mouse IgG1-κ), Mono (GF200),POD Conjugated | BC | R | 05178-34 | 50μg |
| Anti-GST (Mouse IgG2a-κ), Mono (GS019),POD Conjugated | BC | R | 04559-74 | 50μg |
| Anti-V5 (Mouse IgG1-κ), Mono (V5005),POD Conjugated | BC | R | 04578-24 | 50μg |
| Anti-6xHis (Mouse IgG1-κ), Mono (HI192),POD Conjugated | BC | R | 04546-34 | 50μg |
< Agarose Conjugated Tag Antibodies>
| Product name | Grade | Storage | Code No. | Quantily |
|---|---|---|---|---|
| Anti-c-Myc (Mouse IgG1-κ),Mono (MC045), Agarose Conjugated | BC | R | 04145-55 | 500μg |
< Related Products >
| Product name | Grade | Storage | Code No. | Quantily |
|---|---|---|---|---|
| WB Stripping Solution | SP | R | 05364-55 | 500 ml |
| CBB Stain One | SP | RT | 04543-51 | 1 L |
| 04543-64 | 5 L | |||
| Blocking One | SP | R | 03953-95 | 500 ml |
| Pre-stained Protein Markers (Broad Range) | SP | F | 02525-35 | 500 μl |
| Pre-stained Protein Markers (High Range) | SP | F | 26039-75 | 500 μl |
| Pre-stained Protein Markers (Low Range) | SP | F | 28941-75 | 500 μl |
| 28944-74 | 5 x100 μl |
For research use only, not intended for diagnostic or drug use.
(Storage)
A = Cool and dark, RT = Room temperature, F = Freezer, R= Refrigerator,
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