Epitope Tag Antibody
NACALAI TESQUE, INC. carries a family of epitope tag antibodies for the detection and purification of the recombinant proteins. Most of Nacalai's tag antibodies are highly specific mouse monoclonal antibodies.
- Anti-c-Myc(mouse lgG1-κ), Monoclonal (MC045)
- Anti-c-Myc, Agarose Conjugate
- Anti-c-Myc, POD Conjugated
- Anti-GFP(mouse lgG1-κ), Monoclonal(GF200)
- Anti-GFP, POD Conjugated
- Anti-GFP(Rat lgG2a), Monoclonal(GF090R)
- Anti-GFP (Rat IgG2a), Mono (GF090R),CC, Agarose Conjugate
- Anti-GST (Mouse IgG2a-k), Mono (GS019)
- Anti-GST, POD Conjugated
- Anti-HA (Mouse IgG1-κ), Mono (HA124)
- Anti-6xHis (Mouse IgG1a-k), Mono (HI192)
- Anti-6xHis, POD Conjugated
- Anti-DYKDDDDK(Mouse IgG2b-κ), Mono
- Anti-DYKDDDDK (Mouse), Monoclonal Agarose Conjugate
- Anti-V5 (Mouse IgG1-κ), Mono (V5005)
- Anti-V5, POD Conjugated
Anti-c-Myc(mouse lgG1-κ), Monoclonal (MC045)
Clone | MC045 |
Isotype | IgG1-κ(mouse) |
Product form | Liquid |
Immunogen | c-Myc synthetic peptide [EQKLISEEDL] conjugated with KLH |
Application | Western blotting 1:1000 - 1:2000 Immunoprecipitation 1:400 - 1:1000 Immunocytochemistry 1:400 - 1:1000 ELISA 1:2000 - 1:20000 |
Applications
Sample | SH-SY5Y cell in which c-Myc-tagged TCP1β is expressed |
Primary antibody | Anti-c-Myc (Mouse IgG1-κ) (2.5 µg/ml) Anti-Protein X |
Secondary antibody | Alexa Fluor 546-conjugated antibody (1:400) Alexa Fluor 488-conjugated antibody (1:400) |
Data courtesy of RIKEN Brain Science Institute |
Downloads
References
for Western Blotting and Immunoblotting
Title | Author | Journal, Year Vol., Page |
---|---|---|
Estrogen Inhibits Transforming Growth Factor β Signaling by Promoting Smad2/3 Degradation | Ichiaki Ito, Aki Hanyu, Mitsutoshi Wayama, Natsuka Goto, Yoko Katsuno, Shohei Kawasaki, Yuka Nakajima, Masashi Kajiro, Yoko Komatsu, Akiko Fujimura, Ryuichi Hirota, Akiko Murayama, Keiji Kimura, Takeshi Imamura and Junn Yanagisawa | The Journal of Biological Chemistry, 2010 May 7; 285(19): 1474714755 |
Tissue and Cell-Specific Localization of Rice Aquaporins and Their Water Transport Activities | Junko Sakurai, Arifa Ahamed, Mari Murai, Masayoshi Maeshima and Matsuo Uemura | Plant Cell Physiol (2008) 49 (1): 30-39 |
Enzymatic measurement of phosphatidylserine in cultured cells | Shin-ya Morita, Sachimi Shirakawa, Yukiko Kobayashi, Keiko Nakamura, Reiko Teraoka, Shuji Kitagawa and Tomohiro Terada | J Lipid Res. 2012 Feb;53(2):325-30 |
Proteome Analysis of Detergent-Resistant Membranes (DRMs) Associated with OsRac1-Mediated Innate Immunity in Rice | Masayuki Fujiwara, Satoshi Hamada, Minori Hiratsuka, Yoichiro Fukao, Tsutomu Kawasaki and Ko Shimamoto | Plant Cell Physiol (2009) 50 (7): 1191-1200 |
Involvement of Hsp90 in Assembly and Nuclear Import of Influenza Virus RNA Polymerase Subunits | Tadasuke Naito, Fumitaka Momose, Atsushi Kawaguchi and Kyosuke Nagata | JOURNAL OF VIROLOGY, Feb. 2007, p. 13391349 |
Glyceraldehyde-3-phosphate Dehydrogenase Aggregate Formation Participates in Oxidative Stress-induced Cell Death | Hidemitsu Nakajima, Wataru Amano, Takeya Kubo, Ayano Fukuhara, Hideshi Ihara, Yasu-Taka Azuma, Hisao Tajima, Takashi Inui, Akira Sawa, and Tadayoshi Takeuchi | J Biol Chem. 2009 Dec 4;284(49):34331-41 |
Phytochrome B regulates Heading date 1 (Hd1)-mediated expression of rice florigen Hd3a and critical day length in rice | Ryo Ishikawa, Mayumi Aoki, Ken-ichi Kurotani, Shuji Yokoi, Tomoko Shinomura, Makoto Takano and Ko Shimamoto | Mol Genet Genomics. 2011 Jun;285(6):461-70 |
for Immunocytochemistry / Immunohistochemistry
Title | Author | Journal, Year Vol., Page |
---|---|---|
The O-fucosyltransferase O-fut1 is an extracellular component that is essential for the constitutive endocytic trafficking of Notch in Drosophila | Takeshi Sasamura, Hiroyuki O. Ishikawa, Nobuo Sasaki, Syunsuke Higashi, Maiko Kanai, Shiho Nakao, Tomonori Ayukawa, Toshiro Aigaki, Katsuhisa Noda, Eiji Miyoshi, Naoyuki Taniguchi and Kenji Matsuno | Development 134, 1347-1356 (2007) |
Dual Subcellular Localization in the Endoplasmic Reticulum and Peroxisomes and a Vital Role in Protecting against Oxidative Stress of Fatty Aldehyde Dehydrogenase Are Achieved by Alternative Splicing | Bunichiro Ashibe, Toshitake Hirai, Kyoichiro Higashi, Kazuhisa Sekimizu and Kiyoto Motojima | J Biol Chem. 2007 Jul 13;282(28):20763-73 |
Ordering Information
Anti-c-Myc, Agarose Conjugate
Applications
Clone | MC045 |
Isotype | IgG1a-Κ (mouse) |
Size | 0.5 mg |
Ab Protein | ca. 2 mg antibody / ml agarose |
Product form | 1:1 suspension in Dulbecco's PBS(-) |
Cells transfected plasmid DNA including c-Myc Tag are lysised, and cell lysate was proceeded to immunoprecipitation with c-Myc-Agarose antibody. The figure above shows the result of wesetrn blotting analysis after immunoprecipitation.
Antibody
Immunoprecipitate: Anti-c-Myc, Agarose conjugate (clone: MC045) (1:500) Western blotting: Anti-c-Myc (clone: MC045)
Data courtesy of a customer
Downloads
References
for Immunopreciptation
Title | Author | Journal, Year Vol., Page |
---|---|---|
Involvement of Hsp90 in Assembly and Nuclear Import of Influenza Virus RNA Polymerase Subunits | Tadasuke Naito, Fumitaka Momose, Atsushi Kawaguchi and Kyosuke Nagata | JOURNAL OF VIROLOGY, Feb. 2007, p. 13391349 |
The juvenile myoclonic epilepsy-related protein EFHC1 interacts with the redox-sensitive TRPM2 channel linked to cell death | Masahiro Katano, Tomohiro Numata, Kripamoy Aguan, Yuji Hara, Shigeki Kiyonaka, Shinichiro Yamamoto, Takafumi Miki, Seishiro Sawamura, Toshimitsu Suzuki, Kazuhiro Yamakawa and Yasuo Mori | Cell Calcium. 2012 Feb;51(2):179-85 |
Ordering Information
Product Name | Application | Storage | Product No. | PKG Size | Price |
---|---|---|---|---|---|
Anti-c-Myc (Mouse IgG1-Κ), Monoclonal (MC045), AS, Agarose Conjugate | IP | R | 04145-55 | 500µg | Inquire |
(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze
Anti-c-Myc, POD Conjugated
Clone | MC045 |
Isotype | IgG1a-Κ (mouse) |
Product form | Liquid |
Immunogen | c-Myc synthetic peptide [EQKLISEEDL] conjugated with KLH |
Application | Western blotting 1:1000 - 1:2000 ELISA 1:30000 - 1:60000 |
Downloads
References
for Immunoblotting
Title | Author | Journal, Year Vol., Page |
---|---|---|
Physical and Functional Interaction of Transmembrane Thioredoxin-related Protein with Major Histocompatibility Complex Class I Heavy Chain: Redox-based Protein Quality Control and Its Potential Relevance to Immune Responses | Yoshiyuki Matsuo, Hiroshi Masutani, Aoi Son, Shinae Kizaka-Kondoh and Junji Yodoi | Molecular Biology of the Cell, Vol. 20, 45524562 |
Ordering Information
Product Name | Application | Storage | Product No. | PKG Size | Price |
---|---|---|---|---|---|
Anti-c-Myc (Mouse IgG1-Κ), Monoclonal (MC045), AS, POD Conjugated | WB, ELISA | R | 04554-24 | 50µg | Inquire |
(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze
Anti-GFP(mouse lgG1-κ), Monoclonal(GF200)
Clone | GF200 |
GF090R | IgG1-κ(Mouse) |
Product form | Liquid |
Immunogen | His-GFP (full length) fusion protein |
Application | Western blotting 1:1000 - 1:2000 ELISA 1:2000 - 1:20000 |
Applications
Sample | lane 1) HeLa:GFP-H2A-Bbd lane 2) HeLa:GFP-H2B lane 3) HeLa |
Data courtesy of Dr. Kimura H., Horizontal Medical Research Organization, Kyoto University Faculty of Medicine |
Sample | Protein extract from seedlings of Arabidopsis (10mg) Lane1 : Non-transformants (Control) Lane2 : Transformants |
Primary antibody | Anti-GFP(mouse IgG-κ) (1:5000) |
Secondary antibody | Anti-mouse IgG-HRP (1:2000) |
Data courtesy of Dr. Shimada T., Department of Botany, Graduate School of Science, Kyoto University |
Downloads
References
for Western Blotting and Immunoblotting
Title | Author | Journal, Year Vol., Page |
---|---|---|
A novel GTPase, CRAG, mediates promyelocytic leukemia proteinassociated nuclear body formation and degradation of expanded polyglutamine protein | Qingyu Qin, Ryoko Inatome, Azusa Hotta, Masaki Kojima, Hirohei Yamamura, Hirokazu Hirai, Toshihiro Yoshizawa, Hirofumi Tanaka, Kiyoko Fukami and Shigeru Yanagi | J Cell Biol. 2006 Feb 13;172(4):497-504 |
Saccharomyces cerevisiae glucose signalling regulator Mth1p regulates the organellar Na+/H+ exchanger Nhx1p | Keiji Mitsui, Masafumi Matsushita and Hiroshi Kanazawa | Biochem J. 2010 Dec 1;432(2):343-52 |
Membrane-Proximal Region of Glutamate Receptor δ2 Subunit Is Critical for Long-Term Depression and Interaction with Protein Interacting with C Kinase 1 in a Cerebellar Purkinje Neuron | Satoshi Yawata, Hiroshi Tsuchida, Mineko Kengaku and Tomoo Hirano | The Journal of Neuroscience, April 5, 2006 26(14):3626-3633 |
Characterization of a novel posttranslational modification in neuronal nitric oxide synthase by small ubiquitin-related modifier-1 | Masatomo Watanabe, Kouichi Itoh | Biochim Biophys Acta. 2011 Jul;1814(7):900-7 |
Recruitment of PCM1 to the Centrosome by the Cooperative Action of DISC1 and BBS4: A Candidate for Psychiatric Illnesses | Atsushi Kamiya, MD, PhD; Perciliz L. Tan, BSc; Ken-ichiro Kubo, MD, PhD; Caitlin Engelhard, BSc; Koko Ishizuka, MD, PhD; Akiharu Kubo, MD, PhD; Sachiko Tsukita, PhD; Ann E. Pulver, ScD; Kazunori Nakajima, MD, PhD; Nicola G. Cascella, MD; Nicholas Katsanis, PhD; Akira Sawa, MD, PhD | Arch Gen Psychiatry. 2008 September ; 65(9): 9961006 |
Protein Kinase C δ Regulates Ser46 Phosphorylation of p53 Tumor Suppressor in the Apoptotic Response to DNA Damage | Kiyotsugu Yoshida, Hanshao Liu, and Yoshio Miki | J Biol Chem. 2006 Mar 3;281(9):5734-40 |
PIAS3 negatively regulates RANKL-mediated osteoclastogenesis directly in osteoclast precursors and indirectly via osteoblasts | Tomohiro Hikata, Hironari Takaishi, Jiro Takito, Akihiro Hakozaki, Mitsuru Furukawa, Shinichi Uchikawa, Tokuhiro Kimura, Yasunori Okada, Masahito Matsumoto, Akihiko Yoshimura, Riko Nishimura, Sakamuri V. Reddy, Sakamuri V. Reddy, Hiroshi Asahara, and Yoshiaki Toyama | Blood. 2009 Mar 5;113(10):2202-12 |
DYRK2 Is Targeted to the Nucleus and Controls p53 via Ser46 Phosphorylation in the Apoptotic Response to DNA Damage | Naoe Taira, Keishi Nihira, Tomoko Yamaguchi, Yoshio Miki, Kiyotsugu Yoshida | Mol Cell. 2007 Mar 9;25(5):725-38 |
Pim-1 controls NF-κB signalling by stabilizing RelA/p65 | K Nihira, Y Ando, T Yamaguchi, Y Kagami, Y Miki and K Yoshida | Cell Death and Differentiation (2010) 17, 689698 |
Ordering Information
Anti-GFP, POD Conjugated
Clone | GF200 |
GF090R | IgG1-κ(Mouse) |
Product form | Liquid |
Immunogen | His-GFP (full length) fusion protein |
Application | Western blotting 1:1000 - 1:2000 ELISA 1:2000 - 1:4000 |
Downloads
Ordering Information
Product Name | Application | Storage | Product No. | PKG Size | Price |
---|---|---|---|---|---|
Anti-GFP (Mouse IgG1-Κ), Monoclonal (GF200), AS, POD Conjugated | WB, ELISA | R | 05178-34 | 50 µg | Inquire |
(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze
Anti-GFP(Rat lgG2a), Monoclonal(GF090R)
Feature
- Immunohistochemical application
- Rat monoclonal antibody
Clone | GF090R |
GF090R | IgG2a(Rat) |
Product form | Liquid |
Immunogen | His-GFP (full length) fusion protein |
Application | Immunohistochemstry 1:1000 - 1:2000 Western blotting 1:1000 - 1:2000 ELISA 1:2000 - 1:20000 |
Applications
Immunocytochemistry
Sample | Mouse brain (nerve cell) |
Primary antibody | Anti-GFP(Rat IgG2a), Monoclonal (1:1000) RT, O/N |
Secondary antibody | Anti-Rat IgG-Cy3 (1:300) RT, 1hr |
Blocking | 5% normal goat serum / 0.2% TritonX-100 in PBS |
Fixing method | 4% Paraformaldehyde |
Data courtesy of Dr.Yoshihara Y., RIKEN Brain Science Institute
Western blotting
Sample | Lane 1) GFP Lane 2) GFP-14-3-3 (293 cell lysate 50 μg) |
Gel | 5-20% Gradient Gel |
1st antibody | Anti-GFP(Rat IgG2a), Monoclonal (3 μg/ml) O/N |
2nd antibody | Anti-Rat IgG-HRP(1:10000) |
Data courtesy of Dr.Yoshida K., Department of Molecular Genetics,Medical Research Institute, Tokyo Medical and Dental University
Downloads
References
for Immunocytochemistry / Immunohistochemistry
Title | Author | Journal, Year Vol., Page |
---|---|---|
The O-fucosyltransferase O-fut1 is an extracellular component that is essential for the constitutive endocytic trafficking of Notch in Drosophila | Takeshi Sasamura, Hiroyuki O. Ishikawa, Nobuo Sasaki, Syunsuke Higashi, Maiko Kanai, Shiho Nakao, Tomonori Ayukawa, Toshiro Aigaki, Katsuhisa Noda, Eiji Miyoshi, Naoyuki Taniguchi and Kenji Matsuno | Development 134, 1347-1356 (2007) |
Zic1 and Zic3 Regulate Medial Forebrain Development through Expansion of Neuronal Progenitors | Takashi Inoue,1 Maya Ota,1 Miyuki Ogawa,1 Katsuhiko Mikoshiba,2 and Jun Aruga1 | The Journal of Neuroscience, May 16, 2007 27(20):54615473 |
Mammalian Atg2 proteins are essential for autophagosome formation and important for regulation of size and distribution of lipid droplets | Anoop Kumar G. Velikkakath, Taki Nishimura, Eiko Oita, Naotada Ishihara and Noboru Mizushima | Mol Biol Cell. 2012 Mar 23(5):896-909 |
p62 targeting to the autophagosome formation site requires self-oligomerization but not LC3 binding | Eisuke Itakura , Noboru Mizushima | J Cell Biol. 2011 January 10; 192(1): 1727 |
Beclin 1 Forms Two Distinct Phosphatidylinositol 3-Kinase Complexes with Mammalian Atg14 and UVRAG | Eisuke Itakura, Chieko Kishi, Kinji Inoue and Noboru Mizushima | Molecular Biology of the Cell, Vol. 19, 53605372 |
Valosin-containing Protein (VCP) in Novel Feedback Machinery between Abnormal Protein Accumulation and Transcriptional Suppression | Masaaki Koike, Junpei Fukushi, Yuzuru Ichinohe, Naoki Higashimae, Masahiko Fujishiro, Chiyomi Sasaki, Masahiro Yamaguchi, Toshiki Uchihara, Saburo Yagishita, Hiroshi Ohizumi, Seiji Hori and Akira Kakizuka | THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 285, NO. 28, pp. 2173621749 |
Rostral hindbrain patterning involves the direct activation of a Krox20 transcriptional enhancer by Hox/Pbx and Meis factor | Michel A. Wassef, Diane Chomette, Marie Pouilhe, Aline Stedman, Emmanuelle Havis, Carole Desmarquet-Trin Dinh, Sylvie Schneider-Maunoury, Pascale Gilardi-Hebenstreit, Patrick Charnay and Julien Ghislain | Development 135, 3369-3378 (2008) |
Cerebellum morphogenesis: the foliation pattern is orchestrated by multi-cellular anchoring centers | Anamaria Sudarov and Alexandra L Joyner | Neural Development 2007, 2:26 |
X Chromosome Activity in Mouse XX Primordial Germ Cell | Susana M. Chuva de Sousa Lopes, Katsuhiko Hayashi, Tanya C. Shovlin, Will Mifsud, M. Azim Surani, Anne McLaren | PLoS Genet. 2008 Feb;4(2):e30 |
Embryonic stem cells reduce liver fibrosis in CCl4-treated mice | Kei Moriya, Masahide Yoshikawa, Yukiteru Ouji, Ko Saito, Mariko Nishiofuku, Ryosuke Matsuda, Shigeaki Ishizaka and Hiroshi Fukui | Int. J. Exp. Path. (2008), 89, 401409 |
Sonic hedgehog expressing and responding cells generate neuronal diversity in the medial amygdala | Rosalind SE Carney, Jean-Marie Mangin, Lindsay Hayes, Kevin Mansfield, Vitor H Sousa, Gord Fishell, Robert P Machold, Sohyun Ahn, Vittorio Gallo and Joshua G Corbin | Neural Development 2010, 5:14 |
Clonal Production and Organization of Inhibitory Interneurons in the Neocortex | Keith N. Brown, et al. | Science 334, 480 (2011) |
Phospholipase Cγ2 is necessary for separation of blood and lymphatic vasculature in mice | Hirotake Ichise, Taeko Ichise, Osamu Ohtani and Nobuaki Yoshida | Development 136, 191-195 (2009) |
Triple N-Glycosylation in the Long S5-P Loop Regulates the Activation and Trafficking of the Kv12.2 Potassium Channel | Kazushi Kimura, Keiichiro Minatohara, Hisako Nakashima, Yasuaki Nagao, Akira Mizoguchi and Yoshinori Fujiyoshi | J Biol Chem. 2009 Nov 27;284(48):33139-50 |
Sodium-level-sensitive sodium channel Nax is expressed in glial laminate processes in the sensory circumventricular organs | Eiji Watanabe, Takeshi Y. Hiyama, Hidetada Shimizu, Ryuji Kodama, Noriko Hayashi, Seiji Miyata, Yuchio Yanagawa, Kunihiko Obata and Masaharu Noda | Am J Physiol Regul Integr Comp Physiol 290:R568-R576, 2006 |
Transgenic Xenopus with prx1 limb enhancer reveals crucial contribution of MEK/ERK and PI3K/AKT pathways in blastema formation during limb regeneration. | Suzuki M, Satoh A, Ide H, Tamura K | Dev Biol. 2007 Apr 15;304(2):675-86 |
Analysis of Medaka sox9 Orthologue Reveals a Conserved Role in Germ Cell Maintenance | Shuhei Nakamura, Ikuko Watakabe, Toshiya Nishimura, Atsushi Toyoda, Yoshihito Taniguchi and Minoru Tanaka | PLoS One. 2012;7(1):e29982 |
Genetic visualization of the secondary olfactory pathway in Tbx21 transgenic mice | Sachiko Mitsui, Kei M Igarashi, Kensaku Mori and Yoshihiro Yoshihara | Neural Systems & Circuits 2011, 1:5 |
The Germinal Zones of the Basal Ganglia But Not the Septum Generate GABAergic Interneurons for the Cortex | Anna N. Rubin, Fabienne Alfonsi, Michael P. Humphreys, Christina K. P. Choi, Susana F. Rocha, and Nicoletta Kessaris | The Journal of Neuroscience, 8 September 2010, 30(36): 12050-12062 |
Contact-dependent promotion of cell migration by the OL-protocadherinNap1 interaction | Shinsuke Nakao, Anna Platek, Shinji Hirano and Masatoshi Takeichi | J Cell Biol. 2008 July 28; 182(2): 395410 |
Autophagy Is Activated for Cell Survival after Endoplasmic Reticulum Stress | Maiko Ogata, Shin-ichiro Hino, Atsushi Saito, Keisuke Morikawa, Shinichi Kondo, Soshi Kanemoto, Tomohiko Murakami, Manabu Taniguchi, Ichiro Tanii, Kazuya Yoshinaga, Sadao Shiosaka, James A. Hammarback, Fumihiko Urano and Kazunori Imaizumi | Mol Cell Biol. 2006 December; 26(24): 92209231 |
Spatial Genetic Patterning of the Embryonic Neuroepithelium Generates GABAergic Interneuron Diversity in the Adult Cortex | Matthew Fogarty, Matthew Grist, Diego Gelman, Oscar Marn, Vassilis Pachnis and Nicoletta Kessaris | The Journal of Neuroscience, 10 October 2007, 27(41): 10935-10946 |
The Id2+ distal tip lung epithelium contains individual multipotent embryonic progenitor cells | Emma L. Rawlins, Cheryl P. Clark, Yan Xue and Brigid L. M. Hogan | Development 136, 3741-3745 (2009) |
Intra-axonal Patterning: Intrinsic Compartmentalization of the Axonal Membrane in Drosophila Neurons | Takeo Katsuki, Deepak Ailani, Masaki Hiramoto and Yasushi Hiromi | Neuron. 2009 Oct 29;64(2):188-99 |
Multidirectional and multizonal tangential migration of GABAergic interneurons in the developing cerebral cortex | Daisuke H. Tanaka, Kazunori Maekawa, Yuchio Yanagawa, Kunihiko Obata and Fujio Murakami | Development 133, 2167-2176 (2006) |
Laminar and Areal Expression of Unc5d and Its Role in Cortical Cell Survival | Makoto Takemoto, Yuki Hattori, Hong Zhao, Haruka Sato, Atsushi Tamada, Shinji Sasaki, Kazunori Nakajima and Nobuhiko Yamamoto | Cereb Cortex. 2011 Aug;21(8):1925-34 |
Kalrn promoter usage and isoform expression respond to chronic cocaine exposure | Richard E Mains, Drew D Kiraly, Jodi E Eipper-Mains, Xin-Ming Ma and Betty A Eipper | BMC Neuroscience 2011, 12:20 |
Functional reconstruction of NANOS3 expression in the germ cell lineage by a novel transgenic reporter reveals distinct subcellular localizations of NANOS3 | Masashi Yamaji, Takashi Tanaka, Mayo Shigeta, Shinichiro Chuma, Yumiko Saga and Mitinori Saitou | Reproduction. 2010 Feb;139(2):381-93 |
Numb controls E-cadherin endocytosis through p120 catenin with aPKC | Kazuhide Sato, Takashi Watanabe, Shujie Wang, Mai Kakeno, Kenji Matsuzawa, Toshinori Matsui, Keiko Yokoi, Kiyoko Murase, Ikuko Sugiyama, Masayuki Ozawa and Kozo Kaibuchi | Mol. Biol. Cell September 1, 2011 vol. 22 no. 17 3103-3119 |
Neuronal activity is required for the development of specific cortical interneuron subtypes | Natalia V. De Marco Garca, Theofanis Karayannis and Gord Fishell | Nature. 2011 Apr 21;472(7343):351-5 |
In vitro production of fertile sperm from murine spermatogonial stem cell lines | Takuya Sato, Kumiko Katagiri, Tetsuhiro Yokonishi, Yoshinobu Kubota, Kimiko Inoue, Narumi Ogonuki, Shogo Matoba, Atsuo Ogura and Takehiko Ogawa | Nature Communications 2, Article number:472 |
Transgenic Inhibition of Synaptic Transmission Reveals Role of CA3 Output in Hippocampal Learning | Toshiaki Nakashiba, Jennie Z. Young, Thomas J. McHugh, Derek L. Buhl, and Susumu Tonegawa | Science. 2008 Feb 29; 319(5867):1260-4 |
Glioblastoma Formation from Cell Population Depleted of Prominin1-Expressing Cells | Kenji Nishide, Yuka Nakatani, Hiroshi Kiyonari and Toru Kondo | PLoS ONE 4(8): e6869 |
H-, N- and Kras cooperatively regulate lymphatic vessel growth by modulating VEGFR3 expression in lymphatic endothelial cells in mice | Taeko Ichise, Nobuaki Yoshida and Hirotake Ichise | Development 137, 1003-1013 (2010) |
Leftright asymmetric morphogenesis of the anterior midgut depends on the activation of a non-muscle myosin II in Drosophila | Takashi Okumura, Hiroo Fujiwara, Kiichiro Taniguchi, Junpei Kuroda, Naotaka Nakazawa, Mitsutoshi Nakamura, Ryo Hatori, Akira Ishio, Reo Maeda, Kenji Matsuno | Dev Biol. 2010 Aug 15;344(2):693-706 |
Gli2 Is a Novel Regulator of Sox2 Expression in Telencephalic Neuroepithelial Cells | Hiromi Takanaga, Nobuko Tsuchida-Straeten, Kenji Nishide and Akira Watanabe, Hiroyuki Aburatani and Toru Kondo | Stem Cells. 2009 Jan;27(1):165-74 |
Dynein and dynactin components modulate neurodegeneration induced by excitotoxicity | Takeshi Fujiwara, Koji Morimoto, Akiyoshi Kakita and Hitoshi Takahashi | J. Neurochem. (2012) 10.1111/j.1471-4159 |
The Ca2+-binding Protein ALG-2 Is Recruited to Endoplasmic Reticulum Exit Sites by Sec31A and Stabilizes the Localization of Sec31A | Akinori Yamasaki, Katsuko Tani, Akitsugu Yamamoto, Naomi Kitamura and Masayuki Komada | Mol. Biol. Cell November 1, 2006 vol. 17 no. 11 4876-4887 |
Control of Cortical Axon Elongation by a GABA-Driven Ca2+/Calmodulin-Dependent Protein Kinase Cascad | Natsumi Ageta-Ishihara, Sayaka Takemoto-Kimura, Mio Nonaka, Aki Adachi-Morishima, Kanzo Suzuki, Satoshi Kamijo, Hajime Fujii, Tatsuo Mano, Frank Blaeser, Talal A. Chatila, Hidenobu Mizuno, Tomoo Hirano, Yoshiaki Tagawa, Hiroyuki Okuno and Haruhiko Bito | The Journal of Neuroscience, October 28, 2009 29(43):13720 13729 |
Spatially asymmetric reorganization of inhibition establishes a motion-sensitive circuit | Keisuke Yonehara, Kamill Balint, Masaharu Noda, Georg Nagel, Ernst Bamberg and Botond Roska | Nature 469, 407-410 (20 January 2011) |
Identification and developmental analysis of endothelin receptor type-A expressing cells in the mouse kidney | Taro Kitazawa, Takahiro Sato, Koichi Nishiyama, Rieko Asai, Yuichiro Arima, Yasunobu Uchijima, Yukiko Kurihara, Hiroki Kurihara | Gene Expr Patterns. 2011 Oct;11(7):371-7 |
Two Notch Ligands, Dll1 and Jag1, Are Differently Restricted in Their Range of Action to Control Neurogenesis in the Mammalian Spinal Cord | Catarina Ramos, Susana Rocha, Claudia Gaspar, Domingos Henrique | PLoS One. 2010 Nov 24;5(11):e15515 |
LKB1-Mediated Spatial Control of GSK3β and Adenomatous Polyposis Coli Contributes to Centrosomal Forward Movement and Neuronal Migration in the Developing Neocortex | Naoyuki Asada and Kamon Sanada | Journal of Neuroscience (2010)Volume: 30, Issue: 26, Pages: 8852-8865 |
Simple Motor System of the Ascidian Larva: Neuronal Complex Comprising Putative Cholinergic and GABAergic/Glycinergic Neurons | Takeo Horie, Masashi Nakagawa,Yasunori Sasakura, Takehiro G. Kusakabe and Motoyuki Tsuda | Zoological Science 27(2):181-190. 2010 |
Elimination of oncogenic neighbors by JNK-mediated engulfment in Drosophila | Shizue Ohsawa, Kaoru Sugimura, Kyoko Takino, Tian Xu, Atsushi Miyawaki, Tatsushi Igaki | Dev Cell. 2011 Mar 15; 20(3):315-28 |
for Western Blotting and Immunoblotting
Title | Author | Journal, Year Vol., Page |
---|---|---|
Lulu2 regulates the circumferential actomyosin tensile system in epithelial cells through p114RhoGEF | Hiroyuki Nakajima and Takuji Tanoue | J Cell Biol. 2011 October 17; 195(2): 245261 |
RUNX3 interactome reveals novel centrosomal targeting of RUNX family of transcription factors | Linda Shyue Huey Chuang, Soak Kuan Lai, Maki Murata-Hori, Ayumi Yamada, Hoi-Yeung Li, Jayantha Gunaratne and Yoshiaki Ito | Cell Cycle. 2012 May 15;11(10). Pages 1938-1947 |
ARHGAP18, a GTPase-activating protein for RhoA, controls cell shape, spreading, and motility | Masao Maeda, Hitoki Hasegawa, Toshinori Hyodo, Satoko Ito, Eri Asano, Hong Yuang, Kohei Funasaka, Kaoru Shimokata, Yoshinori Hasegawa, Michinari Hamaguchi, and Takeshi Senga | Mol Biol Cell. 2011 Oct;22(20):3840-52 |
Role of Palladin Phosphorylation by Extracellular Signal-Regulated Kinase in Cell Migration | Eri Asano, Masao Maeda, Hitoki Hasegawa, Satoko Ito, Toshinori Hyodo, Hong Yuan, Masahide Takahashi, Michinari Hamaguchi, Takeshi Senga | PloS one. 01/2011; 6(12):e29338 |
Novel BRCA2-interacting protein BJ-HCC-20A inhibits the induction of apoptosis in response to DNA damage | Tomiyoshi G, Nakanishi A, Takenaka K, Yoshida K, Miki Y | Cancer Sci. 2008 Apr;99(4):747-54 |
Enhanced Deacetylation of p53 by the Anti-apoptotic Protein HSCO in Association with Histone Deacetylase 1 | Hisako Higashitsuji, Hiroaki Higashitsuji, Tomoko Masuda, Yu Liu, Katsuhiko Itoh, and Jun Fujita | J Biol Chem. 2007 May 4;282(18):13716-25 |
ERK1/2 phosphorylate GEF-H1 to enhance its guanine nucleotide exchange activity toward RhoA | Shuh-hei Fujishiro, Susumu Tanimura, Shogo Mure, Yuji Kashimoto, Kazushi Watanabe and Michiaki Kohno | Biochemical and Biophysical Research Communications, 2008 Mar 28;368(1):162-7 |
Atg9A Protein, an Autophagy-related Membrane Protein, Is Localized in the Neurons of Mouse Brains | Hirosumi Tamura, Masahiro Shibata, Masato Koike, Mitsuho Sasaki and Yasuo Uchiyama | Histochem Cytochem. 2010 May; 58(5): 443453 |
Cooperative Role of Nectin-Nectin and Nectin-Afadin Interactions in Formation of Nectin-based Cell-Cell Adhesion | Souichi Kurita, Hisakazu Ogita and Yoshimi Takai | J Biol Chem. 2011 Oct 21;286(42):36297-303. |
ALCAM (CD166) Is a Surface Marker for Early Murine Cardiomyocytes | Hirokazu Hirata, Yoshinobu Murakami, Yoshiaki Miyamoto, Mako Tosaka, Kayoko Inoue, Ayako Nagahashi, Lars Martin Jakt, Takayuki Asahara, Hiroo Iwata, Yoshiki Sawa and Shin Kawamata | Cells Tissues Organs 2006;184:172-180 |
Ordering Information
Anti-GFP (Rat IgG2a), Mono (GF090R),CC, Agarose Conjugate
Applications
Western blotting
Samples: HEK-293 cell lysate expressing either EGFP or EGFPMyosin(1 ml each) Immunoprecipitation was performed with Anti-GFP agarose conjugate (20 µl) at 4°C for 2 hours.
Data courtesy of Dr. A. Kakizuka, Laboratory of Functional Biology, Graduate School of Biostudies, Kyoto University
Downloads
Ordering Information
Product Name | Application | Storage | Product No. | PKG Size | Price |
---|---|---|---|---|---|
Anti-GFP (Rat IgG2a), Monoclonal(GF090R), CC, Agarose Conjugate | IP | R | 06083-05 | 500 µg | Inquire |
(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze
Anti-GST (Mouse IgG2a-k), Mono (GS019)
Clone | GS019 |
Isotype | IgG2a-κ(mouse) |
Product form | Liquid |
Immunogen | Glutathione - S - Transferase(GST) |
Application | Western blotting 1:1000 - 1:2000 Immunoprecipitation 1:400 - 1:1000 ELISA 1:2000 - 1:20000 |
Applications
Sample:
- Lysate from E. coli expressioning GST protein
- Glutathione Sepharose flow-through fraction
- Glutathione Sepharose elution fraction
Gel | 12.5% SDS-PAGE |
Blocking | Blocking One (Nacalai Tesque) /1 hr at RT |
1st Antibody | Anti-GST (Mouse IgG2a-κ) (1:2000 with Blocking One) /1 hr at RT |
Wash | TBS-T (5 min x 3) |
2nd Antibody | Anti-Mouse Ig-HRP (1:1000 with TBS-T)/30 min at RT |
Wash | TBS-T (5 min x 3) |
Detection | Luminol Reagent (Santa Cruz) |
Data courtesy of Dr. Y. Shimizu, The Institute of Medical Science, The University of Tokyo
Downloads
References
for Western Blotting / Immunoblotting
Title | Author | Journal, Year Vol., Page |
---|---|---|
Lulu2 regulates the circumferential actomyosin tensile system in epithelial cells through p114RhoGEF | Hiroyuki Nakajima and Takuji Tanoue | J Cell Biol. 2011 October 17; 195(2): 245261 |
Protein Kinase C δ Regulates Ser46 Phosphorylation of p53 Tumor Suppressor in the Apoptotic Response to DNA Damage | Kiyotsugu Yoshida, Hanshao Liu, and Yoshio Miki | J Biol Chem. 2006 Mar 3;281(9):5734-40 |
DYRK2 Is Targeted to the Nucleus and Controls p53 via Ser46 Phosphorylation in the Apoptotic Response to DNA Damage | Naoe Taira, Keishi Nihira, Tomoko Yamaguchi, Yoshio Miki, Kiyotsugu Yoshida | Mol Cell. 2007 Mar 9;25(5):725-38Masatoshi Hara, Development 136 (10): 1687-1696 |
LOVASTATIN INSENSITIVE 1, a Novel Pentatricopeptide Repeat Protein, is a Potential Regulatory Factor of Isoprenoid Biosynthesis in Arabidopsis | Keiko Kobayashi, Masashi Suzuki, Jianwei Tang, Noriko Nagata, Kiyoshi Ohyama, Hikaru Seki, Reiko Kiuchi, Yasuko Kaneko, Miki Nakazawa, Minami Matsui, Shogo Matsumoto, Shigeo Yoshida and Toshiya Muranaka | Plant Cell Physiol. 48(2): 322331 (2007) |
Regulation of melanin-concentrating hormone receptor 1 signaling by RGS8 with the receptor third intracellular loop | Mayumi Miyamoto-Matsubara, Osamu Saitoh, Kei Maruyama, Yoshimi Aizaki, Yumiko Saito | Cell Signal. 2008 Nov;20(11):2084-94 |
Molecular diversity of the two sugar-binding sites of the β-trefoil lectin HA33/C (HA1) from Clostridium botulinum type C neurotoxin | Toshio Nakamura, Takashi Tonozuka, Sakae Ito, Yoichi Takeda, Ryutaro Sato, Ichiro Matsuo, Yukishige Ito, Keiji Oguma, Atsushi Nishikawa | Arch Biochem Biophys. 2011 Aug 1;512(1):69-77 |
Tyrosine Phosphorylation of 3BP2 Regulates B Cell Receptor-mediated Activation of NFAT | Upasana Shukla, Tomoko Hatan, Kenji Nakashima, Kazuhiro Ogi and Kiyonao Sada | J Biol Chem. 2009 December 4; 284(49): 3371933728 |
ATM Augments Nuclear Stabilization of DYRK2 by Inhibiting MDM2 in the Apoptotic Response to DNA Damage | Naoe Taira, Hiroyuki Yamamoto, Tomoko Yamaguchi, Yoshio Miki and Kiyotsugu Yoshida | J Biol Chem. 2010 February 12; 285(7): 49094919 |
Leukotriene B4 Augments and Restores Fc γRs-dependent Phagocytosis in Macrophages | Fuyuki Okamoto, Kazuko Saek, Hideki Sumimoto, Sho Yamasaki and Takehiko Yokomizo | J Biol Chem. 2010 December 24; 285(52): 4111341121 |
The yeast VAP homolog Scs2p has a phosphoinositide-binding ability that is correlated with its activity | Satoshi Kagiwada and Misa Hashimoto | Biochemical and Biophysical Research Communications. 2007 Dec 28;364(4):870-6 |
Intracellular protein delivery activity of peptides derived from insulin-like growth factor binding proteins 3 and 5 | Natsuko Goda, Takeshi Tenno, Kosuke Inomata, Masahiro Shirakawa, Toshiki Tanaka, Hidekazu Hiroaki | Exp Cell Res. 2008 Aug 1;314(13):2352-61 |
The common phospholipid-binding activity of the N-terminal domains of PEX1 and VCP/p97 | Kumiko Shiozawa, Natsuko Goda, Toshiyuki Shimizu, Kenji Mizuguch, Naomi Kondo, Nobuyuki Shimozawa, Masahiro Shirakawa and Hidekazu Hiroaki | FEBS J. 2006 Nov;273(21):4959-71 |
Novel BRCA2-interacting protein BJ-HCC-20A inhibits the induction of apoptosis in response to DNA damage | Go Tomiyoshi, Akira Nakanishi, Katsuya Takenaka, Kiyotsugu Yoshida and Yoshio Miki | Cancer Sci. 2008 Apr;99(4):747-54 |
Human protein factory for converting the transcriptome into an in vitroexpressed proteome | Naoki Goshima, Yoshifumi Kawamura, Akiko Fukumoto, Aya Miura, Reiko Honma, Ryohei Satoh, Ai Wakamatsu, Jun-ichi Yamamoto, Kouichi Kimura, Tetsuo Nishikawa, Taichi Andoh, Yuki Iida, Kumiko Ishikawa, Emi Ito, Naoko Kagawa, Chie Kaminaga, Kei-ichi Kanehori, Bunsei Kawakami, Kiyokazu Kenmochi, Rie Kimura, Miki Kobayashi, Toshihiro Kuroita, Hisashi Kuwayama, Yukio Maruyama, Kiyoshi Matsuo, Kazuyoshi Minami, Mariko Mitsubori, Masatoshi Mori, Riyo Morishita, Atsushi Murase, Akira Nishikawa, Shigemichi Nishikawa, Toshihiko Okamoto, Noriko Sakagami, Yutaka Sakamoto, Yukari Sasaki, Tomoe Seki, Saki Sono, Akio Sugiyama, Tsuyoshi Sumiya, Tomoko Takayama, Yukiko Takayama, Hiroyuki Takeda, Takushi Togashi, Kazuhide Yahata, Hiroko Yamada, Yuka Yanagisawa, Yaeta Endo, Fumio Imamoto, Yasutomo Kisu, Shigeo Tanaka, Takao Isogai, Jun-ichi Imai, Shinya Watanabe and Nobuo Nomura | Nature Methods 5, 1011 - 1017 (2008) |
Pim-1 controls NF-κB signalling by stabilizing RelA/p65 | K Nihira, Y Ando, T Yamaguchi, Y Kagami, Y Miki and K Yoshida | Cell Death and Differentiation (2010) 17, 689698 |
Ordering Information
Anti-GST, POD Conjugated
Clone | GS019 |
Isotype | IgG2a-κ(mouse) |
Product form | Liquid |
&Immunogen | Glutathione-s-Transferase (GST) |
Application | Western blotting 1:4000 - 1:8000 ELISA 1:30000 - 1:60000 |
Downloads
References
for Immunoblotting
Title | Author | Journal, Year Vol., Page |
---|---|---|
Glycoside Hydrolase Family 89α-N-acetylglucosaminidase from Clostridium perfringens Specifically Acts on GlcNAc 1,4Galβ1R at the Non-reducing Terminus of O-Glycans in Gastric Mucin | Masaya Fujita, Akiko Tsuchida, Akiko Hirata, Natsumi Kobayash, Kohtaro Goto, Kenji Osumi, Yuriko Hirose, Jun Nakayama, Takashi Yamano, Hisashi Ashida and Mamoru Mizuno | J Biol Chem. 2011 February 25; 286(8): 64796489 |
Ordering Information
Product Name | Application | Storage | Product No. | PKG Size | Price |
---|---|---|---|---|---|
Anti-GST (Mouse IgG2a-κ), Monoclonal (GS019), AS, POD Conjugated | WB, ELISA | R | 04559-74 | 50µg | Inquire |
(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze
Anti-HA (Mouse IgG1-κ), Mono (HA124)
Clone | HA124 |
GF090R | IgG1-k (Mouse) |
Product form | Liquid |
Immunogen | Gulutathione-S-Transferase (GST) -HA[YPYDVPDYA-COOH] fusion protein |
Application | Western Blotting 1:10000-1:30000 ELISA 1:2000-1:20000 |
Applications
Western blotting
Sample
- E. coli lysate
- NH2-(GST)(T7)(HSV)(c-Myc)(VSV)
(Glu-Glu)(V5)(E-tag)(DDDDK)(S-tag)(HA)
(6xHis)-COOHNH (GST) (abcam, #ab5395) 250 ng - NH2-(GST)(HA)-COOH (PIERCE, #23613) 20 ng
- NH2-(GST)(HA)-COOH (PIERCE, #23613) 100 ng
- M.W. marker
1st antibody | Anti-HA (Mouse IgG1-κ), Mono (HA124), 1:10000, 60min |
2nd antibody | Anti-Mouse IgG(Rat IgG), CC, POD Conjugated 1:25000, 60min |
Detection | Chemi-Lumi One (Product No. 05027-20), 1 min |
Immunoprecipitation and gel staining
- Clarified bacteria lysate
+HA-tagged GST 1 µg
+Anti-HA (Mouse IgG1-κ), Mono (HA124), 5 µg
+Protein G Agarose (50% Suspention) 0.05 ml - E coli lysate
+NH2-(GST)(HA)-COOH(PIERECE, #23613) 1 µg
Detection : Silver staining
Downloads
References
for Immunoblotting
Title | Author | Journal, Year Vol., Page |
---|---|---|
Interaction between Periostin and BMP-1 Promotes Proteolytic Activation of Lysyl Oxidase | Takumi Maruhashi, Isao Kii, Mitsuru Saito and Akira Kudo | THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 285, NO. 17, pp. 13294-13303 |
A novel vanadium transporter of the Nramp family expressed at the vacuole of vanadium-accumulating cells of the ascidian Ascidia sydneiensis samea | Tatsuya Ueki, Nobuaki Furuno and Hitoshi Michibata | Biochim Biophys Acta. 2011 Apr;1810(4):457-64 |
Intra-axonal Patterning: Intrinsic Compartmentalization of the Axonal Membrane in Drosophila Neuron | Takeo Katsuki, Deepak Ailani, Masaki Hiramoto and Yasushi Hiromi | Neuron. 2009 Oct 29;64(2):188-99 |
Red1 promotes the elimination of meiosis-specific mRNAs in vegetatively growing fission yeast | Tomoyasu Sugiyama and Rie Sugioka-Sugiyama | The EMBO Journal (2011) 30, 1027-1039 |
Ordering Information
Anti-6xHis (Mouse IgG1a-k), Mono (HI192)
Clone | HI192 |
GF090R | IgG1-k (Mouse) |
Product form | Liquid |
Immunogen | 6xHis synthetic peptide [HHHHHH] conjugated with KLH |
Application | Western Blotting 1:1000-1:2000 ELISA 1:2000-1:20000 |
Applications
Western Blotting
Sample:
Lysate from Sf9 cells in which His-tagged Drosophila PTEN is expressed
Filter | : FluoroTrans [PALL] |
Blocking | : 5% non-fat dry milk/PBS (30 min) |
1st antibody | : Anti-6xHis (Mouse IgG1-), Monoclonal (HI192) |
Wash | : 0.25% Tween-20/TBS (10 min x 3) |
2nd antibody | : Anti-Mouse IgG-HRP (1:1000) /2% BSA /0.25% Tween-20 /TBS (30 min) |
Wash | :0.25% Tween-20/TBS (10 min x 5) |
Detection | : Luminol Reagent (Santa Cruz) |
Data courtesy of Dr. A. Maehama, The Tokyo Metropolitan Institute of Medical Science (RINSHOKEN)
Downloads
References
for Immunoprecipitation
Title | Author | Journal, Year Vol., Page |
---|---|---|
Reduction of transforming growth factor-βtype II receptor is caused by the enhanced ubiquitin-dependent degradation in human renal cell carcinoma | Hirotaka Fukasawa, Tatsuo Yamamoto, Yoshihide Fujigaki, Taro Misaki, Naro Ohashi, Tatsuya Takayama, Sayuri Suzuki, Soichi Mugiya, Toshiaki Oda, Chiharu Uchida, Kyoko Kitagawa, Takayuki Hattori, Hidetoshi Hayashi, Seiichiro Ozono, Masatoshi Kitagawa and Akira Hishida | Int J Cancer. 2010 Oct 1;127(7):1517-25 |
Ordering Information
Anti-6xHis, POD Conjugated
Clone | HI192 |
Isotype | IgG1a-Κ (mouse) |
Product form | Liquid |
Immunogen | 6xHis synthetic peptide [HHHHHH] conjugated with KLH |
Application | Western blot ting 1:1000 - 1:2000 ELISA 1:30000 - 1:60000 |
Each concentration of GFP-His6 protein synthesized by in vitro translation were apllied 2.5 μl to gel.
Applications
Western blotting
Lane 1 | Sample: | GFP-His6, undiluted solution |
1st Antibody: | Anti-His6 produced by A company, 1:100,00 | |
Lane 2 | Sample: | GFP-His6, 10-fold dilution |
1st Antibody: | Anti-His6 produced by A company, 1:100,000 | |
Lane 3 | Sample: | GFP-His6, undiluted solution |
1st Antibody: | Anti-6xHis, POD conjugated (04546-34), 1:100,000 | |
Lane 4 | Sample: | GFP-His6, 10-fold dilution |
1st Antibody: | Anti-6xHis, POD conjugated (04546-34), 1:100,000 |
Downloads
References
for Western Blotting / Immunoblotting
Title | Author | Journal, Year Vol., Page |
---|---|---|
Reduction of transforming growth factor-β type II receptor is caused by the enhanced ubiquitin-dependent degradation in human renal cell carcinoma | Hirotaka Fukasawa, Tatsuo Yamamoto, Yoshihide Fujigaki, Taro Misaki, Naro Ohashi, Tatsuya Takayama, Sayuri Suzuki, Soichi Mugiya, Toshiaki Oda, Chiharu Uchida, Kyoko Kitagawa, Takayuki Hattori, Hidetoshi Hayashi, Seiichiro Ozono, Masatoshi Kitagawa and Akira Hishida | Int J Cancer. 2010 Oct 1;127(7):1517-25 |
Numb controls E-cadherin endocytosis through p120 catenin with aPKC | Kazuhide Sato, Takashi Watanabe, Shujie Wang, Mai Kakeno, Kenji Matsuzawa, Toshinori Matsui, Keiko Yokoi, Kiyoko Murase, Ikuko Sugiyama, Masayuki Ozawa and Kozo Kaibuchi | Mol. Biol. Cell September 1, 2011 vol. 22 no. 17 3103-3119 |
Longistatin, a novel EF-hand protein from the ixodid tick Haemaphysalis longicornis, is required for acquisition of host blood-meals | Anisuzzaman, M. Khyrul Islam, Takeharu Miyoshi, M. Abdul Alim, Takeshi Hatta, Kayoko Yamaji, Yasunobu Matsumoto, Kozo Fujisaki and Naotoshi Tsuji | Int J Parasitol. 2010 May;40(6):721-9 |
Protein Crosslinking by Transglutaminase Controls Cuticle Morphogenesis in Drosophila | Toshio Shibata, Shigeru Ariki, Naoaki Shinzawa, Ryuta Miyaji, Haruka Suyama, Miyuki Sako, Nobuyuki Inomata, Takumi Koshiba, Hirotaka Kanuka and Shun-ichiro Kawabata | PLoS One. 2010 Oct 18;5(10):e13477 |
Genetic Modification of Bacillus subtilis for Production of D-chiro-Inositol, an Investigational Drug Candidate for Treatment of Type 2 Diabetes and Polycystic Ovary Syndrome | Ken-ichi Yoshida, Masanori Yamaguchi, Tetsuro Morinaga, Maya Ikeuchi, Masaki Kinehara and Hitoshi Ashida | Appl Environ Microbiol. 2006 February; 72(2): 13101315 |
Coupled cytoplasmic transcription-and-translation-a method of choice for heterologous gene expression in Xenopus oocytes | Alexander A. Tokmakov, Eiko Matsumoto, Mikako Shirouzu and Shigeyuki Yokoyama | J Biotechnol. 2006 Mar 9;122(1):5-15 |
Tandem multimer expression of angiotensin I-converting enzyme inhibitory peptide in Escherichia coli | Fida, Hasan M; Kumada, Yoichi; Terashima, Masaaki; Katsuda, Tomohisa; Katoh, Shigeo | Biotechnol J. 2009 Sep;4(9):1345-56 |
myo-Inositol Catabolism in Bacillus subtilis | Ken-ichi Yoshida, Masanori Yamaguchi, Tetsuro Morinaga, Masaki Kinehara, Maya Ikeuchi, Hitoshi Ashida and Yasutaro Fujita | The Journal of Biological Chemistry, 283, 10415-10424 |
Activation of T7 RNA polymerase in Xenopus oocytes and cell-free extracts | Alexander A. Tokmakov and Yasuo Fukami | Genes Cells. 2010 Nov;15(11):1136-44 |
Molecular cloning and characterization of Nop25, a novel nucleolar RNA binding protein, highly conserved in vertebrate species | Shunji Suzuki, Motoko Kanno, Takashi Fujiwara, Hironobu Sugiyama, Akiko Yokoyama, Hisaaki Takahashi and Junya Tanaka | Exp Cell Res. 2006 Apr 15;312(7):1031-41 |
A novel GTPase, CRAG, mediates promyelocytic leukemia protein-associated nuclear body formation and degradation of expanded polyglutamine protein | Qingyu Qin, Ryoko Inatome, Azusa Hotta, Masaki Kojima, Hirohei Yamamura and Hirokazu Hirai | J Cell Bio. 2006 Feb 13:172(4):497-504 |
Ordering Information
Product Name | Application | Storage | Product No. | PKG Size | Price |
---|---|---|---|---|---|
Anti-6xHis (Mouse IgG1-Κ), Monoclonal (HI192), AS, POD Conjugated | WB, ELISA | R | 04546-34 | 50 µg | Inquire |
(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze
Anti-DYKDDDDK(Mouse IgG2b-κ), Mono
Clone | 5A8E5 |
Isotype | IgG2b-κ (Mouse) |
Product form | Liquid |
Specificity | Anti-DYKDDDDK recognizes C-terminal, N-terminal and internal tagged fusion proteins |
Concentration | 0.5 mg/ml, lyophilized with PBS, pH7.4, containing 0.02% sodium azide. |
Immunogen | A synthetic peptide (DYKDDDDK) coupled KLH |
Application | Western Blotting 0.1-1.0 µg/ml Immunoprecipitation 1 µg/ml Immunofluorescent 1 µg/ml ELISA 0.05-0.2 µg/ml |
Applications
Western blotting
Sample | Lane 1. N-terminal DYKDDDDK-tagged fusion protein Lane 2. Internal DYKDDDDK-tagged fusion protein Lane 3. C-terminal DYKDDDDK-tagged fusion protein |
1st Antibody | A. Anti-DYKDDDDK(Mouse IgG2b-κ), Mono (5A8E5) 1 mg/ml B. Company S, Anti-DYKDDDDK Tag mAb (M2) 1 mg/ml |
2nd Antibody | Goat anti-Mouse IgG IRDyeTM 800 Conjugated |
Western blotting
Sample | Lane 1-3. Multiple Tag (Purified) (400 ng, 80 ng, 16 ng) Lane 4. 293 cell lysate 10 ml |
1st antibody | A. Anti-DYKDDDDK(Mouse IgG2b-κ), Mono (5A8E5) 1 mg/ml B. Company S, Anti-DYKDDDDK Tag mAb (M2) 1 mg/ml |
2nd antibody | Goat anti-Mouse IgG IRDyeTM 800 Conjugated |
Downloads
Ordering Information
Product Name | Application | Storage | Product No. | PKG Size | Price |
---|---|---|---|---|---|
Anti-DYKDDDDK (Mouse IgG2b-κ), Mono | WB, IP IF, ELISA |
R | NU01102 | 200 µg | Inquire |
(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze
Anti-DYKDDDDK (Mouse), Monoclonal Agarose Conjugate
Applications
Western Blotting
Lane 1. DYKDDDDK tagged fusion protein input control
Lane 2. Anti-DYKDDDDK, Agarose Conjugated
Lane 3. DYKDDDDK tagged fusion protein was precipitated by
Anti-DYKDDDDK, agarose conjugated.
Clone | 6H8E4 |
Degree of substitution | Approx. 8.5 mg Anti-DYKDDDDK mAb/ml settled resin |
Capacity | > 1 mg/ml settled resin |
Product form | 1x PBS containing 50% glycerol |
Downloads
Ordering Information
Product Name | Application | Storage | Product No. | PKG Size | Price |
---|---|---|---|---|---|
Anti-DYKDDDDK (Mouse), Monoclonal Agarose Conjugate | IP | R | NU01103 | 1 ml | Inquire |
(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze
Anti-V5 (Mouse IgG1-κ), Mono (V5005)
Clone | V5005 |
Isotype | IgG1-κ(mouse) |
Product form | Liquid |
Immunogen | V5 synthetic peptide [GKPIPNPLLGLDST] conjugated with KLH |
Application | Western blotting 1:1000 - 1:2000 Immunoprecipitation 1:400 - 1:1000 ELISA 1:2000 - 1:20000 |
Applications
Western blotting, Immunoprecipitation
(A) Western blotting VE-Card.-V5 | |
Sample | Lysate from 293T cell in which V5-tagged (C-terminal) pCDNA dest40-VE-cadherin is expressed |
Antibody | Anti-V5 (Mouse IgG1-κ) (1:5000) |
(B) Immunoprecipitation VE-Card. -V5 | |
Sample | Lysate (A) |
Antibody | Anti-V5 (Mouse IgG1-κ) (1μl) |
Western blotting | Anti-V5 (Mouse IgG1-κ) (1:2000) |
Downloads
References
for Immunostaining
Title | Author | Journal, Year Vol., Page |
---|---|---|
Activation of the small GTPase Rac1 by a specific guanine-nucleotide-exchange factor suffices to induce glucose uptake into skeletal-muscle cells | Shuji Ueda, Tohru Kataoka and Takaya Satoh | Biol. Cell (2008) 100, 645657 |
Oxidized LDL Receptor LOX-1 Binds to C-Reactive Protein and Mediates Its Vascular Effects | Yoshiko Fujita, Akemi Kakino, Norihisa Nishimichi, Saburo Yamaguchi, Yuko Sato, Sachiko Machida, Luciano Cominacini, Yves Delneste, Haruo Matsuda and Tatsuya Sawamura | Clinical Chemistry 55:2 285294 (2009) |
C-Reactive Protein Uptake by Macrophage Cell Line via Class-A Scavenger Receptor | Yoshiko Fujita, Akemi Kakino, Mariko Harada-Shiba, Yuko Sato, Kazunori Otsui, Ryo Yoshimoto and Tatsuya Sawamura | Clinical Chemistry 56:3 478481 (2010) |
A novel mutation of ALK2, L196P, found in the most benign case of fibrodysplasia ossificans progressiva activates BMP-specific intracellular signaling equivalent to a typical mutation, R206H | Satoshi Ohte, Masashi Shin, Hiroki Sasanuma, Katsumi Yoneyama, Masumi Akita, Kenji Ikebuchi, Eijiro Jimi, Yuichi Maruki, Masaru Matsuoka, Akira Namba, Hiroshi Tomoda, Yasushi Okazaki, Akira Ohtake, Hiromi Oda, Ichiro Owan, Tetsuya Yoda, Hirokazu Furuya, Jyunji Kamizono, Hiroshi Kitoh, Yasuharu Nakashima, Takafumi Susami, Nobuhiko Haga, Tetsuo Komori and Takenobu Katagiri | Biochem Biophys Res Commun. 2011 Apr 1;407(1):213-8 |
for Immunoprecipitation
Title | Author | Journal, Year Vol., Page |
---|---|---|
Phosphorylation-Dependent Protein Interaction with Trypanosoma brucei 14-3-3 Proteins that Display Atypical Target Recognition | Masahiro Inoue, Kouichi Yasuda, Haruki Uemura, Natsumi Yasaka, Hiroshi Inoue, Yoshitatsu Sei, Nobuo Horikoshi, Toshihide Fukuma | PLoS One. 2010 Dec 21;5(12):e15566 |
for Western Blotting / ELISA
Title | Author | Journal, Year Vol., Page |
---|---|---|
Interaction between PKR and PACT mediated by LPS-inducible NF-κB in human gingival cells | Kaya Yoshida, Hirohiko Okamura, Yumi Hoshino, Masayuki Shono, Masami Yoshioka, Daisuke Hinode and Hideo Yoshida | Journal of Cellular Biochemistry 113:165173 (2012) |
Calcium-dependent platelet aggregation through the interaction between phosphatidylserine and LOX-1 | Kazunori Otsui, Makoto Kakutani, Koji Yuasa, Atushi Nakano, Chang-Ping Hu, Akemi Kakino, Yuko Sato, Yoshiko Fujita, Yosuke Kawase, Kou-ichi Jishage, Hiroshi Suzuki, Jawahar L. Mehta, Mitsuhiro Yokoyama, Ken-ichi Hirata, Nobutaka Inoue, and Tatsuya Sawamura | Cardiovasc Res. 2010 Dec 1;88(3):547 |
The orphan nuclear receptor GCNF recruits DNA methyltransferase for Oct-3/4 silencing | Noriko Sato, Mitsumasa Kondo and Ken-ichi Arai | Biochemical and Biophysical Research Communications 344 (2006) 845851 |
Ordering Information
Anti-V5, POD Conjugated
Clone | GF200 |
Isotype | IgG1-Κ (mouse) |
Product form | Liquid |
Immunogen | V5 synthetic peptide [GKPIPNPLLGLDST] conjugated with KLH |
Application | Western blotting 1:1000 - 1:2000 ELISA 1:8000 - 1:16000 |
References
for Immunoblotting
Title | Author | Journal, Year Vol., Page |
---|---|---|
Physical and Functional Interaction of Transmembrane Thioredoxin-related Protein with Major Histocompatibility Complex Class I Heavy Chain: Redox-based Protein Quality Control and Its Potential Relevance to Immune Responses | Yoshiyuki Matsuo, Hiroshi Masutani, Aoi Son, Shinae Kizaka-Kondoh and Junji Yodoi | Molecular Biology of the Cell, Vol. 20, 45524562 |
Ordering Information
Product Name | Application | Storage | Product No. | PKG Size | Price |
---|---|---|---|---|---|
Anti-V5 (Mouse IgG1-κ), Monoclonal (V5005), AS, POD Conjugated | WB, ELISA | R | 04578-24 | 50 µg | Inquire |
(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze