NACALAI TESQUE, INC.

NACALAI TESQUE, INC. carries a family of epitope tag antibodies for the detection and purification of the recombinant proteins. Most of Nacalai’s tag antibodies are highly specific mouse monoclonal antibodies.

• Anti-GFP(Rat lgG2a), Monoclonal(GF090R)
• Anti-c-Myc(mouse lgG1-κ), Monoclonal
• Anti-GFP(mouse lgG1-κ), Monoclonal(GF200)
• Anti-GST(mouse lgG2a-κ), Monoclonal(GS019)
• Anti-V5(mouse lgG1-κ), Monoclonal(V5005)
• Anti-6xHis(Mouse lgG1-κ), Monoclonal(HI192)
• Reference List
• Ordering Infirmation

Anti-GFP(Rat lgG2a), Monoclonal(GF090R)
Feature Immunohistochemical application Rat monoclonal antibody

Clone	GF090R
GF090R	IgG2a(Rat)
Product form	Liquid
Immunogen	Liquid
Application	Immunohistochemstry 1:1000 - 1:2000 Western blotting 1:1000 - 1:2000 ELISA 1:2000 - 1:20000

Immunocytochemistry

Sample	Mouse brain (nerve cell)
Primary antibody	Anti-GFP(Rat IgG2a), Monoclonal (1:1000) RT, O/N
Secondary antibody	Anti-Rat IgG-Cy3 (1:300) RT, 1hr
Blocking	5% normal goat serum / 0.2% TritonX-100 in PBS
Fixing method	4% Paraformaldehyde

Data courtesy of Dr.Yoshihara Y., RIKEN Brain Science Institute

Western blotting

	Sample Lane 1) GFP Lane 2) GFP-14-3-3 (293 cell lysate 50 μg) Gel 5-20% Gradient Gel Primary antibody Anti-GFP(Rat IgG2a), Monoclonal (3 μg/ml) O/N Secondary antibody Anti-Rat IgG-HRP(1:10000)
Data courtesy of Dr.Yoshida K., Department of Molecular Genetics,Medical Research Institute, Tokyo Medical and Dental University

Reference

1. Nakamura, M. et al. Molecular Vision 16, 425-437(2010)
2. Nishide, K. et al. PLoS ONE 4(8), e6869(2009)
3. Nagao, M. et al. The Journal of Cell Biology 183(7), 1243-1257(2008)
4. Ono, K. et al. Development Biology 320(2), 356-468(2008)
5. Nakashiba T. et al. Science 319(5867), 1260-1264 (2008)
6. Batista-Brito R et al. The Journal of Neuroscience 28(15), 3966-3975 (2008)
7. Esumi S. et al. Neuroscience Research 60(4), 439-451 (2008)
8. Fogarty M. et al. The Journal of Neuroscience 27(41), 10935-10946 (2007)
9. Sasamura T. et al. Development 134, 1347-1356 (2007)
10. Sato Y. et al. The Journal of Neuroscience 27(7), 1606-1615 (2007)
11. Yamada M. et al. Stem Cells 25(3), 562-570 (2007)
12. Togashi H. et al. The Journal of Cell Biology 174, 141-151 (2006)
13. Ogata M. et al. Molecular and Cell Biology 26(24), 9220-9231 (2006)
14. Sato Y. et al. The Journal of Neuroscience 25(20), 4889-4897 (2005)

Anti-c-Myc(mouse lgG1-κ), Monoclonal

Clone	MC045
Isotype	IgG1-κ(mouse)
Product form	Liquid
Immunogen	c-Myc synthetic peptide [EQKLISEEDL] conjugated with KLH
Application	Western blotting 1:1000 - 1:2000 Immunoprecipitation 1:400 - 1:1000 Immunocytochemistry 1:400 - 1:1000 ELISA 1:2000 - 1:20000

Immunocytochemistry

Sample	SH-SY5Y cell in which c-Myc-tagged TCP1β is expressed
Primary antibody	Anti-c-Myc (Mouse IgG1-κ) (2.5 μg/ml) Anti-Protein X
Secondary antibody	Alexa Fluor 546-conjugated antibody (1:400) Alexa Fluor 488-conjugated antibody (1:400)
Data courtesy of RIKEN Brain Science Institute

Sample	HeLa cell in which c-Myc-tagged(N-terminal) hnRNP A1 is expressed
Primary antibody	Anti-c-Myc (Mouse IgG1-κ) (1:1000)
Secondary antibody	FITC-conjugated goat affinity purified f(ab')2 fragment to mouse IgG (whole molecule) (1:200)
Fixation method	2% Formaldehyde in PBS (RT, 30min)
Permeabilization	Acetone (-20°C, 3min)
Blocking	3% BSA in PBS (RT)
Data courtesy of Dr. Kataoka N., Department of Genetics and Molecular Biology, Laboratry of Biochemistry, Institute for Virus Research, Kyoto University.

Anti-GFP(mouse lgG1-κ), Monoclonal(GF200)

Clone	GF200
Isotype	IgG1-κ(Mouse)
Product form	Liquid
Immunogen	His-GFP (full length) fusion protein
Application	Western blotting 1:1000 - 1:2000 ELISA 1:2000 - 1:20000

Western blotting

Sample	lane 1) HeLa:GFP-H2A-Bbd lane 2) HeLa:GFP-H2B lane 3) HeLa
Data courtesy of Dr. Kimura H., Horizontal Medical Research Organization, Kyoto University Faculty of Medicine

Western blotting

Sample	Protein extract from seedlings of Arabidopsis (10mg) Lane1 : Non-transformants (Control) Lane2 : Transformants
Primary antibody	Anti-GFP(mouse IgG-κ) (1:5000)
Secondary antibody	Anti-mouse IgG-HRP (1:2000)
Data courtesy of Dr. Shimada T., Department of Botany, Graduate School of Science, Kyoto University

Anti-GST(mouse lgG2a-κ), Monoclonal(GS019)

Clone	GS019
Isotype	IgG2a-κ(mouse)
Product form	Liquid
Immunogen	Glutathione - S - Transferase(GST)
Application	Western blotting 1:1000 - 1:2000 Immunoprecipitation 1:400 - 1:1000 ELISA 1:2000 - 1:20000

Western blotting

Gel	12.5% SDS-PAGE
Blocking	Blocking One (Nacalai Tesque) / 1 hr at RT
Primary antibody	anti-GST (Mouse IgG2a-κ),(GS019) (1:2000 with Blocking One) / 1 hr at RT
Secondary antibody	anti-mouse Ig-HRP(1:1000 with TBS-T) / 30 min at RT
Wash	TBS-T (5 min x 3)
Detection	Luminol Reagent (Santa Cruz)
Sample	lane 1: Lysate from E.coli expressioning GST protein lane 2: Glutathione Sepharose flow-through fraction lane 3: Glutathione Sepharose elution fraction
Data courtesy of Dr. Shimizu Y., The Institute of Medical Science, The University of Tokyo

Anti-V5(mouse lgG1-κ), Monoclonal(V5005)

Clone	V5005
Isotype	IgG1-κ(mouse)
Product form	Liquid
Immunogen	V5 synthetic peptide [GKPIPNPLLGLDST] conjugated with KLH
Application	Western blotting 1:1000 - 1:2000 Immunoprecipitation 1:400 - 1:1000 ELISA 1:2000 - 1:20000

Western blotting, Immunoprecipitation

(A) Western blotting   VE-Card.-V5
Sample	Lysate from 293T cell in which V5-tagged (C-terminal) pCDNA dest40-VE-cadherin is expressed
Antibody	Anti-V5 (Mouse IgG1-κ) (1:5000)

(B) Immunoprecipitation   VE-Card. -V5
Sample	Lysate (A)
Antibody	Anti-V5 (Mouse IgG1-κ) (1μl)
Western blotting	Anti-V5 (Mouse IgG1-κ) (1:2000)

Anti-6xHis(Mouse lgG1-κ), Monoclonal(HI192)

Clone	HI192
Isotype	IgG1-κ(mouse)
Product form	Liquid
Immunogen	6xHis synthetic peptide [HHHHHH] conjugated with KLH
Application	Western blotting 1:1000 - 1:2000 ELISA 1:2000 - 1:20000
Filter	FluoroTrans[PALL]
Blocking	5% non-fat dry milk/PBS (30 min)
Primary antibody	anti-6xHis (Mouse IgG1-κ),Monoclonal (HI192)
Wash	.25% Tween-20/TBS (10 min x 3)
Secondary antibody	anti-mouse IgG-HRP(1:1000) / 2% BSA / 0.25% Tween-20/TBS (30 min)
Wash	0.25% Tween-20/TBS (10 min x 5)
Detection	Luminol Reagent (Santa Cruz)
Sample	lysate from Sf 9 cells in which His-tagged Drosophila PTEN is expressed
Data courtesy of Dr. Maehama A., The Tokyo Metropolitan Institute of Medical Science (RINSHOKEN)

<< Ordering Information Tag Antibodies >>

Product name	Grade	Storage	Code No.	Quantily	Price
Anti-GFP (Rat IgG2a), Monoclonal (GF090R)	BC	R	04404-26	50μg	Inquire
			04404-84	200μg	Inquire
Anti-c-Myc (Mouse IgG1-κ), Monoclonal (MC045)	BC	R	04362-76	50μg	Inquire
			04362-34	200μg	Inquire
Anti-GFP (Mouse IgG1-κ), Monoclonal (GF200)	BC	R	04363-66	50μg	Inquire
			04363-24	200μg	Inquire
Anti-GST (Mouse IgG2a-κ), Monoclonal (GS019)	BC	R	04435-84	50μg	Inquire
			04435-26	200μg	Inquire
Anti-V5 (Mouse IgG1-κ), Monoclonal (V5005)	BC	R	04434-94	50μg	Inquire
			04434-36	200μg	Inquire
Anti-6xHis (Mouse IgG1-κ), Monoclonal (HI192)	BC	R	04428-26	50μg	Inquire
			04428-84	200μg	Inquire

< POD Conjugated Tag Antibodies >

Product name	Grade	Storage	Code No.	Quantily	Price
Anti-c-Myc (Mouse IgG1-κ),Mono (MC045),POD Conjugated	BC	R	04554-24	50μg	Inquire
Anti-GFP (Mouse IgG1-κ), Mono (GF200),POD Conjugated	BC	R	05178-34	50μg	Inquire
Anti-GST (Mouse IgG2a-κ), Mono (GS019),POD Conjugated	BC	R	04559-74	50μg	Inquire
Anti-V5 (Mouse IgG1-κ), Mono (V5005),POD Conjugated	BC	R	04578-24	50μg	Inquire
Anti-6xHis (Mouse IgG1-κ), Mono (HI192),POD Conjugated	BC	R	04546-34	50μg	Inquire

< Agarose Conjugated Tag Antibodies>

Product name	Grade	Storage	Code No.	Quantily	Price
Anti-c-Myc (Mouse IgG1-κ),Mono (MC045), Agarose Conjugated	BC	R	04145-55	500μg	Inquire

Printed version (PDF 1.29 MB)

< Related Products >

Product name	Grade	Storage	Code No.	Quantily	Price
WB Stripping Solution	SP	R	05364-55	500 ml	Inquire
CBB Stain One	SP	RT	04543-51	1 L	Inquire
			04543-64	5 L	Inquire
Blocking One	SP	R	03953-95	500 ml	Inquire
Pre-stained Protein Markers (Broad Range)	SP	F	02525-35	500 μl	Inquire
Pre-stained Protein Markers (High Range)	SP	F	26039-75	500 μl	Inquire
Pre-stained Protein Markers (Low Range)	SP	F	28941-75	500 μl	Inquire
			28944-74	5 x100 μl	Inquire

For research use only, not intended for diagnostic or drug use.

(Storage)
A = Cool and dark, RT = Room temperature, F = Freezer, R= Refrigerator,