Products

Epitope Tag Antibody

NACALAI TESQUE, INC. carries a family of epitope tag antibodies for the detection and purification of the recombinant proteins. Most of Nacalai's tag antibodies are highly specific mouse monoclonal antibodies.

Anti-c-Myc(mouse lgG1-κ), Monoclonal (MC045)

Clone MC045
Isotype IgG1-κ(mouse)
Product form Liquid
Immunogen c-Myc synthetic peptide [EQKLISEEDL] conjugated with KLH
Application Western blotting 1:1000 - 1:2000
Immunoprecipitation 1:400 - 1:1000
Immunocytochemistry 1:400 - 1:1000
ELISA 1:2000 - 1:20000

Applications

Sample SH-SY5Y cell in which c-Myc-tagged TCP1β is expressed
Primary antibody Anti-c-Myc (Mouse IgG1-κ) (2.5 µg/ml) Anti-Protein X
Secondary antibody Alexa Fluor 546-conjugated antibody (1:400)
Alexa Fluor 488-conjugated antibody (1:400)
Data courtesy of RIKEN Brain Science Institute

Downloads

Epitope Tag Antibodies pdf (0.8 MB)

References

for Western Blotting and Immunoblotting

Title Author Journal, Year Vol., Page
Estrogen Inhibits Transforming Growth Factor β Signaling by Promoting Smad2/3 Degradation Ichiaki Ito, Aki Hanyu, Mitsutoshi Wayama, Natsuka Goto, Yoko Katsuno, Shohei Kawasaki, Yuka Nakajima, Masashi Kajiro, Yoko Komatsu, Akiko Fujimura, Ryuichi Hirota, Akiko Murayama, Keiji Kimura, Takeshi Imamura and Junn Yanagisawa The Journal of Biological Chemistry, 2010 May 7; 285(19): 1474714755
Tissue and Cell-Specific Localization of Rice Aquaporins and Their Water Transport Activities Junko Sakurai, Arifa Ahamed, Mari Murai, Masayoshi Maeshima and Matsuo Uemura Plant Cell Physiol (2008) 49 (1): 30-39
Enzymatic measurement of phosphatidylserine in cultured cells Shin-ya Morita, Sachimi Shirakawa, Yukiko Kobayashi, Keiko Nakamura, Reiko Teraoka, Shuji Kitagawa and Tomohiro Terada J Lipid Res. 2012 Feb;53(2):325-30
Proteome Analysis of Detergent-Resistant Membranes (DRMs) Associated with OsRac1-Mediated Innate Immunity in Rice Masayuki Fujiwara, Satoshi Hamada, Minori Hiratsuka, Yoichiro Fukao, Tsutomu Kawasaki and Ko Shimamoto Plant Cell Physiol (2009) 50 (7): 1191-1200
Involvement of Hsp90 in Assembly and Nuclear Import of Influenza Virus RNA Polymerase Subunits Tadasuke Naito, Fumitaka Momose, Atsushi Kawaguchi and Kyosuke Nagata JOURNAL OF VIROLOGY, Feb. 2007, p. 13391349
Glyceraldehyde-3-phosphate Dehydrogenase Aggregate Formation Participates in Oxidative Stress-induced Cell Death Hidemitsu Nakajima, Wataru Amano, Takeya Kubo, Ayano Fukuhara, Hideshi Ihara, Yasu-Taka Azuma, Hisao Tajima, Takashi Inui, Akira Sawa, and Tadayoshi Takeuchi J Biol Chem. 2009 Dec 4;284(49):34331-41
Phytochrome B regulates Heading date 1 (Hd1)-mediated expression of rice florigen Hd3a and critical day length in rice Ryo Ishikawa, Mayumi Aoki, Ken-ichi Kurotani, Shuji Yokoi, Tomoko Shinomura, Makoto Takano and Ko Shimamoto Mol Genet Genomics. 2011 Jun;285(6):461-70

for Immunocytochemistry / Immunohistochemistry

Title Author Journal, Year Vol., Page
The O-fucosyltransferase O-fut1 is an extracellular component that is essential for the constitutive endocytic trafficking of Notch in Drosophila Takeshi Sasamura, Hiroyuki O. Ishikawa, Nobuo Sasaki, Syunsuke Higashi, Maiko Kanai, Shiho Nakao, Tomonori Ayukawa, Toshiro Aigaki, Katsuhisa Noda, Eiji Miyoshi, Naoyuki Taniguchi and Kenji Matsuno Development 134, 1347-1356 (2007)
Dual Subcellular Localization in the Endoplasmic Reticulum and Peroxisomes and a Vital Role in Protecting against Oxidative Stress of Fatty Aldehyde Dehydrogenase Are Achieved by Alternative Splicing Bunichiro Ashibe, Toshitake Hirai, Kyoichiro Higashi, Kazuhisa Sekimizu and Kiyoto Motojima J Biol Chem. 2007 Jul 13;282(28):20763-73

Ordering Information

Product Name Application Storage Product No. PKG Size  Price
Anti-c-Myc (Mouse IgG1-κ), Monoclonal (MC045) WB, IP, ICC, ELISA R 04362-76 50µg Inquire
04362-34 200µg Inquire

(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze

Anti-c-Myc, Agarose Conjugate

Applications

Clone MC045
Isotype IgG1a-Κ (mouse)
Size 0.5 mg
Ab Protein ca. 2 mg antibody / ml agarose
Product form 1:1 suspension in Dulbecco's PBS(-)

Cells transfected plasmid DNA including c-Myc Tag are lysised, and cell lysate was proceeded to immunoprecipitation with c-Myc-Agarose antibody. The figure above shows the result of wesetrn blotting analysis after immunoprecipitation.

Antibody
Immunoprecipitate: Anti-c-Myc, Agarose conjugate (clone: MC045) (1:500) Western blotting: Anti-c-Myc (clone: MC045)

Data courtesy of a customer

Downloads

Epitope Tag Antibodies pdf (0.8 MB)

References

for Immunopreciptation

Title Author Journal, Year Vol., Page
Involvement of Hsp90 in Assembly and Nuclear Import of Influenza Virus RNA Polymerase Subunits Tadasuke Naito, Fumitaka Momose, Atsushi Kawaguchi and Kyosuke Nagata JOURNAL OF VIROLOGY, Feb. 2007, p. 13391349
The juvenile myoclonic epilepsy-related protein EFHC1 interacts with the redox-sensitive TRPM2 channel linked to cell death Masahiro Katano, Tomohiro Numata, Kripamoy Aguan, Yuji Hara, Shigeki Kiyonaka, Shinichiro Yamamoto, Takafumi Miki, Seishiro Sawamura, Toshimitsu Suzuki, Kazuhiro Yamakawa and Yasuo Mori Cell Calcium. 2012 Feb;51(2):179-85

Ordering Information

Product Name Application Storage Product No. PKG Size  Price
Anti-c-Myc (Mouse IgG1-Κ), Monoclonal (MC045), AS, Agarose Conjugate IP R 04145-55 500µg Inquire

(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze

Anti-c-Myc, POD Conjugated

Clone MC045
Isotype IgG1a-Κ (mouse)
Product form Liquid
Immunogen c-Myc synthetic peptide [EQKLISEEDL]
conjugated with KLH
Application Western blotting 1:1000 - 1:2000
ELISA 1:30000 - 1:60000

Downloads

Epitope Tag Antibodies pdf (0.8 MB)

References

for Immunoblotting

Title Author Journal, Year Vol., Page
Physical and Functional Interaction of Transmembrane Thioredoxin-related Protein with Major Histocompatibility Complex Class I Heavy Chain: Redox-based Protein Quality Control and Its Potential Relevance to Immune Responses Yoshiyuki Matsuo, Hiroshi Masutani, Aoi Son, Shinae Kizaka-Kondoh and Junji Yodoi Molecular Biology of the Cell, Vol. 20, 45524562

Ordering Information

Product Name Application Storage Product No. PKG Size  Price
Anti-c-Myc (Mouse IgG1-Κ), Monoclonal (MC045), AS, POD Conjugated WB, ELISA R 04554-24 50µg Inquire

(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze

Anti-GFP(mouse lgG1-κ), Monoclonal(GF200)

Clone GF200
GF090R IgG1-κ(Mouse)
Product form Liquid
Immunogen His-GFP (full length) fusion protein
Application Western blotting 1:1000 - 1:2000
ELISA 1:2000 - 1:20000

Applications

Sample lane 1) HeLa:GFP-H2A-Bbd
lane 2) HeLa:GFP-H2B
lane 3) HeLa
Data courtesy of Dr. Kimura H., Horizontal Medical Research Organization,
Kyoto University Faculty of Medicine

Sample Protein extract from seedlings of Arabidopsis (10mg)
Lane1 : Non-transformants (Control)
Lane2 : Transformants
Primary antibody Anti-GFP(mouse IgG-κ) (1:5000)
Secondary antibody Anti-mouse IgG-HRP (1:2000)
Data courtesy of Dr. Shimada T., Department of Botany, Graduate School of Science, Kyoto University

Downloads

Epitope Tag Antibodies pdf (0.8 MB)

References

for Western Blotting and Immunoblotting

Title Author Journal, Year Vol., Page
A novel GTPase, CRAG, mediates promyelocytic leukemia proteinassociated nuclear body formation and degradation of expanded polyglutamine protein Qingyu Qin, Ryoko Inatome, Azusa Hotta, Masaki Kojima, Hirohei Yamamura, Hirokazu Hirai, Toshihiro Yoshizawa, Hirofumi Tanaka, Kiyoko Fukami and Shigeru Yanagi J Cell Biol. 2006 Feb 13;172(4):497-504
Saccharomyces cerevisiae glucose signalling regulator Mth1p regulates the organellar Na+/H+ exchanger Nhx1p Keiji Mitsui, Masafumi Matsushita and Hiroshi Kanazawa Biochem J. 2010 Dec 1;432(2):343-52
Membrane-Proximal Region of Glutamate Receptor δ2 Subunit Is Critical for Long-Term Depression and Interaction with Protein Interacting with C Kinase 1 in a Cerebellar Purkinje Neuron Satoshi Yawata, Hiroshi Tsuchida, Mineko Kengaku and Tomoo Hirano The Journal of Neuroscience, April 5, 2006 26(14):3626-3633
Characterization of a novel posttranslational modification in neuronal nitric oxide synthase by small ubiquitin-related modifier-1 Masatomo Watanabe, Kouichi Itoh Biochim Biophys Acta. 2011 Jul;1814(7):900-7
Recruitment of PCM1 to the Centrosome by the Cooperative Action of DISC1 and BBS4: A Candidate for Psychiatric Illnesses Atsushi Kamiya, MD, PhD; Perciliz L. Tan, BSc; Ken-ichiro Kubo, MD, PhD; Caitlin Engelhard, BSc; Koko Ishizuka, MD, PhD; Akiharu Kubo, MD, PhD; Sachiko Tsukita, PhD; Ann E. Pulver, ScD; Kazunori Nakajima, MD, PhD; Nicola G. Cascella, MD; Nicholas Katsanis, PhD; Akira Sawa, MD, PhD Arch Gen Psychiatry. 2008 September ; 65(9): 9961006
Protein Kinase C δ Regulates Ser46 Phosphorylation of p53 Tumor Suppressor in the Apoptotic Response to DNA Damage Kiyotsugu Yoshida, Hanshao Liu, and Yoshio Miki J Biol Chem. 2006 Mar 3;281(9):5734-40
PIAS3 negatively regulates RANKL-mediated osteoclastogenesis directly in osteoclast precursors and indirectly via osteoblasts Tomohiro Hikata, Hironari Takaishi, Jiro Takito, Akihiro Hakozaki, Mitsuru Furukawa, Shinichi Uchikawa, Tokuhiro Kimura, Yasunori Okada, Masahito Matsumoto, Akihiko Yoshimura, Riko Nishimura, Sakamuri V. Reddy, Sakamuri V. Reddy, Hiroshi Asahara, and Yoshiaki Toyama Blood. 2009 Mar 5;113(10):2202-12
DYRK2 Is Targeted to the Nucleus and Controls p53 via Ser46 Phosphorylation in the Apoptotic Response to DNA Damage Naoe Taira, Keishi Nihira, Tomoko Yamaguchi, Yoshio Miki, Kiyotsugu Yoshida Mol Cell. 2007 Mar 9;25(5):725-38
Pim-1 controls NF-κB signalling by stabilizing RelA/p65 K Nihira, Y Ando, T Yamaguchi, Y Kagami, Y Miki and K Yoshida Cell Death and Differentiation (2010) 17, 689698

Ordering Information

Product Name Application Storage Product No. PKG Size Price
Anti-GFP (Mouse IgG1-Κ), Monoclonal (GF200) WB, ELISA R 04363-66 50 µg Inquire
04363-24 200 µg Inquire

(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze

Anti-GFP, POD Conjugated

Clone GF200
GF090R IgG1-κ(Mouse)
Product form Liquid
Immunogen His-GFP (full length) fusion protein
Application Western blotting 1:1000 - 1:2000
ELISA 1:2000 - 1:4000

Downloads

Epitope Tag Antibodies pdf (0.8 MB)

Ordering Information

Product Name Application Storage Product No. PKG Size  Price
Anti-GFP (Mouse IgG1-Κ), Monoclonal (GF200), AS, POD Conjugated WB, ELISA R 05178-34 50 µg Inquire

(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze

Anti-GFP(Rat lgG2a), Monoclonal(GF090R)

Feature

  • Immunohistochemical application
  • Rat monoclonal antibody
Clone GF090R
GF090R IgG2a(Rat)
Product form Liquid
Immunogen His-GFP (full length) fusion protein
Application Immunohistochemstry 1:1000 - 1:2000
Western blotting 1:1000 - 1:2000
ELISA 1:2000 - 1:20000

Applications

Immunocytochemistry

Sample Mouse brain (nerve cell)
Primary antibody Anti-GFP(Rat IgG2a), Monoclonal (1:1000) RT, O/N
Secondary antibody Anti-Rat IgG-Cy3 (1:300) RT, 1hr
Blocking 5% normal goat serum / 0.2% TritonX-100 in PBS
Fixing method 4% Paraformaldehyde

Data courtesy of Dr.Yoshihara Y., RIKEN Brain Science Institute

Western blotting

Sample Lane 1) GFP
Lane 2) GFP-14-3-3
(293 cell lysate 50 μg)
Gel 5-20% Gradient Gel
1st antibody Anti-GFP(Rat IgG2a), Monoclonal (3 μg/ml) O/N
2nd antibody Anti-Rat IgG-HRP(1:10000)

Data courtesy of Dr.Yoshida K., Department of Molecular Genetics,Medical Research Institute, Tokyo Medical and Dental University

Downloads

Epitope Tag Antibodies pdf (0.8 MB)

References

for Immunocytochemistry / Immunohistochemistry

Title Author Journal, Year Vol., Page
The O-fucosyltransferase O-fut1 is an extracellular component that is essential for the constitutive endocytic trafficking of Notch in Drosophila Takeshi Sasamura, Hiroyuki O. Ishikawa, Nobuo Sasaki, Syunsuke Higashi, Maiko Kanai, Shiho Nakao, Tomonori Ayukawa, Toshiro Aigaki, Katsuhisa Noda, Eiji Miyoshi, Naoyuki Taniguchi and Kenji Matsuno Development 134, 1347-1356 (2007)
Zic1 and Zic3 Regulate Medial Forebrain Development through Expansion of Neuronal Progenitors Takashi Inoue,1 Maya Ota,1 Miyuki Ogawa,1 Katsuhiko Mikoshiba,2 and Jun Aruga1 The Journal of Neuroscience, May 16, 2007 27(20):54615473
Mammalian Atg2 proteins are essential for autophagosome formation and important for regulation of size and distribution of lipid droplets Anoop Kumar G. Velikkakath, Taki Nishimura, Eiko Oita, Naotada Ishihara and Noboru Mizushima Mol Biol Cell. 2012 Mar 23(5):896-909
p62 targeting to the autophagosome formation site requires self-oligomerization but not LC3 binding Eisuke Itakura , Noboru Mizushima J Cell Biol. 2011 January 10; 192(1): 1727
Beclin 1 Forms Two Distinct Phosphatidylinositol 3-Kinase Complexes with Mammalian Atg14 and UVRAG Eisuke Itakura, Chieko Kishi, Kinji Inoue and Noboru Mizushima Molecular Biology of the Cell, Vol. 19, 53605372
Valosin-containing Protein (VCP) in Novel Feedback Machinery between Abnormal Protein Accumulation and Transcriptional Suppression Masaaki Koike, Junpei Fukushi, Yuzuru Ichinohe, Naoki Higashimae, Masahiko Fujishiro, Chiyomi Sasaki, Masahiro Yamaguchi, Toshiki Uchihara, Saburo Yagishita, Hiroshi Ohizumi, Seiji Hori and Akira Kakizuka THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 285, NO. 28, pp. 2173621749
Rostral hindbrain patterning involves the direct activation of a Krox20 transcriptional enhancer by Hox/Pbx and Meis factor Michel A. Wassef, Diane Chomette, Marie Pouilhe, Aline Stedman, Emmanuelle Havis, Carole Desmarquet-Trin Dinh, Sylvie Schneider-Maunoury, Pascale Gilardi-Hebenstreit, Patrick Charnay and Julien Ghislain Development 135, 3369-3378 (2008)
Cerebellum morphogenesis: the foliation pattern is orchestrated by multi-cellular anchoring centers Anamaria Sudarov and Alexandra L Joyner Neural Development 2007, 2:26
X Chromosome Activity in Mouse XX Primordial Germ Cell Susana M. Chuva de Sousa Lopes, Katsuhiko Hayashi, Tanya C. Shovlin, Will Mifsud, M. Azim Surani, Anne McLaren PLoS Genet. 2008 Feb;4(2):e30
Embryonic stem cells reduce liver fibrosis in CCl4-treated mice Kei Moriya, Masahide Yoshikawa, Yukiteru Ouji, Ko Saito, Mariko Nishiofuku, Ryosuke Matsuda, Shigeaki Ishizaka and Hiroshi Fukui Int. J. Exp. Path. (2008), 89, 401409
Sonic hedgehog expressing and responding cells generate neuronal diversity in the medial amygdala Rosalind SE Carney, Jean-Marie Mangin, Lindsay Hayes, Kevin Mansfield, Vitor H Sousa, Gord Fishell, Robert P Machold, Sohyun Ahn, Vittorio Gallo and Joshua G Corbin Neural Development 2010, 5:14
Clonal Production and Organization of Inhibitory Interneurons in the Neocortex Keith N. Brown, et al. Science 334, 480 (2011)
Phospholipase Cγ2 is necessary for separation of blood and lymphatic vasculature in mice Hirotake Ichise, Taeko Ichise, Osamu Ohtani and Nobuaki Yoshida Development 136, 191-195 (2009)
Triple N-Glycosylation in the Long S5-P Loop Regulates the Activation and Trafficking of the Kv12.2 Potassium Channel Kazushi Kimura, Keiichiro Minatohara, Hisako Nakashima, Yasuaki Nagao, Akira Mizoguchi and Yoshinori Fujiyoshi J Biol Chem. 2009 Nov 27;284(48):33139-50
Sodium-level-sensitive sodium channel Nax is expressed in glial laminate processes in the sensory circumventricular organs Eiji Watanabe, Takeshi Y. Hiyama, Hidetada Shimizu, Ryuji Kodama, Noriko Hayashi, Seiji Miyata, Yuchio Yanagawa, Kunihiko Obata and Masaharu Noda Am J Physiol Regul Integr Comp Physiol 290:R568-R576, 2006
Transgenic Xenopus with prx1 limb enhancer reveals crucial contribution of MEK/ERK and PI3K/AKT pathways in blastema formation during limb regeneration. Suzuki M, Satoh A, Ide H, Tamura K Dev Biol. 2007 Apr 15;304(2):675-86
Analysis of Medaka sox9 Orthologue Reveals a Conserved Role in Germ Cell Maintenance Shuhei Nakamura, Ikuko Watakabe, Toshiya Nishimura, Atsushi Toyoda, Yoshihito Taniguchi and Minoru Tanaka PLoS One. 2012;7(1):e29982
Genetic visualization of the secondary olfactory pathway in Tbx21 transgenic mice Sachiko Mitsui, Kei M Igarashi, Kensaku Mori and Yoshihiro Yoshihara Neural Systems & Circuits 2011, 1:5
The Germinal Zones of the Basal Ganglia But Not the Septum Generate GABAergic Interneurons for the Cortex Anna N. Rubin, Fabienne Alfonsi, Michael P. Humphreys, Christina K. P. Choi, Susana F. Rocha, and Nicoletta Kessaris The Journal of Neuroscience, 8 September 2010, 30(36): 12050-12062
Contact-dependent promotion of cell migration by the OL-protocadherinNap1 interaction Shinsuke Nakao, Anna Platek, Shinji Hirano and Masatoshi Takeichi J Cell Biol. 2008 July 28; 182(2): 395410
Autophagy Is Activated for Cell Survival after Endoplasmic Reticulum Stress Maiko Ogata, Shin-ichiro Hino, Atsushi Saito, Keisuke Morikawa, Shinichi Kondo, Soshi Kanemoto, Tomohiko Murakami, Manabu Taniguchi, Ichiro Tanii, Kazuya Yoshinaga, Sadao Shiosaka, James A. Hammarback, Fumihiko Urano and Kazunori Imaizumi Mol Cell Biol. 2006 December; 26(24): 92209231
Spatial Genetic Patterning of the Embryonic Neuroepithelium Generates GABAergic Interneuron Diversity in the Adult Cortex Matthew Fogarty, Matthew Grist, Diego Gelman, Oscar Marn, Vassilis Pachnis and Nicoletta Kessaris The Journal of Neuroscience, 10 October 2007, 27(41): 10935-10946
The Id2+ distal tip lung epithelium contains individual multipotent embryonic progenitor cells Emma L. Rawlins, Cheryl P. Clark, Yan Xue and Brigid L. M. Hogan Development 136, 3741-3745 (2009)
Intra-axonal Patterning: Intrinsic Compartmentalization of the Axonal Membrane in Drosophila Neurons Takeo Katsuki, Deepak Ailani, Masaki Hiramoto and Yasushi Hiromi Neuron. 2009 Oct 29;64(2):188-99
Multidirectional and multizonal tangential migration of GABAergic interneurons in the developing cerebral cortex Daisuke H. Tanaka, Kazunori Maekawa, Yuchio Yanagawa, Kunihiko Obata and Fujio Murakami Development 133, 2167-2176 (2006)
Laminar and Areal Expression of Unc5d and Its Role in Cortical Cell Survival Makoto Takemoto, Yuki Hattori, Hong Zhao, Haruka Sato, Atsushi Tamada, Shinji Sasaki, Kazunori Nakajima and Nobuhiko Yamamoto Cereb Cortex. 2011 Aug;21(8):1925-34
Kalrn promoter usage and isoform expression respond to chronic cocaine exposure Richard E Mains, Drew D Kiraly, Jodi E Eipper-Mains, Xin-Ming Ma and Betty A Eipper BMC Neuroscience 2011, 12:20
Functional reconstruction of NANOS3 expression in the germ cell lineage by a novel transgenic reporter reveals distinct subcellular localizations of NANOS3 Masashi Yamaji, Takashi Tanaka, Mayo Shigeta, Shinichiro Chuma, Yumiko Saga and Mitinori Saitou Reproduction. 2010 Feb;139(2):381-93
Numb controls E-cadherin endocytosis through p120 catenin with aPKC Kazuhide Sato, Takashi Watanabe, Shujie Wang, Mai Kakeno, Kenji Matsuzawa, Toshinori Matsui, Keiko Yokoi, Kiyoko Murase, Ikuko Sugiyama, Masayuki Ozawa and Kozo Kaibuchi Mol. Biol. Cell September 1, 2011 vol. 22 no. 17 3103-3119
Neuronal activity is required for the development of specific cortical interneuron subtypes Natalia V. De Marco Garca, Theofanis Karayannis and Gord Fishell Nature. 2011 Apr 21;472(7343):351-5
In vitro production of fertile sperm from murine spermatogonial stem cell lines Takuya Sato, Kumiko Katagiri, Tetsuhiro Yokonishi, Yoshinobu Kubota, Kimiko Inoue, Narumi Ogonuki, Shogo Matoba, Atsuo Ogura and Takehiko Ogawa Nature Communications 2, Article number:472
Transgenic Inhibition of Synaptic Transmission Reveals Role of CA3 Output in Hippocampal Learning Toshiaki Nakashiba, Jennie Z. Young, Thomas J. McHugh, Derek L. Buhl, and Susumu Tonegawa Science. 2008 Feb 29; 319(5867):1260-4
Glioblastoma Formation from Cell Population Depleted of Prominin1-Expressing Cells Kenji Nishide, Yuka Nakatani, Hiroshi Kiyonari and Toru Kondo PLoS ONE 4(8): e6869
H-, N- and Kras cooperatively regulate lymphatic vessel growth by modulating VEGFR3 expression in lymphatic endothelial cells in mice Taeko Ichise, Nobuaki Yoshida and Hirotake Ichise Development 137, 1003-1013 (2010)
Leftright asymmetric morphogenesis of the anterior midgut depends on the activation of a non-muscle myosin II in Drosophila Takashi Okumura, Hiroo Fujiwara, Kiichiro Taniguchi, Junpei Kuroda, Naotaka Nakazawa, Mitsutoshi Nakamura, Ryo Hatori, Akira Ishio, Reo Maeda, Kenji Matsuno Dev Biol. 2010 Aug 15;344(2):693-706
Gli2 Is a Novel Regulator of Sox2 Expression in Telencephalic Neuroepithelial Cells Hiromi Takanaga, Nobuko Tsuchida-Straeten, Kenji Nishide and Akira Watanabe, Hiroyuki Aburatani and Toru Kondo Stem Cells. 2009 Jan;27(1):165-74
Dynein and dynactin components modulate neurodegeneration induced by excitotoxicity Takeshi Fujiwara, Koji Morimoto, Akiyoshi Kakita and Hitoshi Takahashi J. Neurochem. (2012) 10.1111/j.1471-4159
The Ca2+-binding Protein ALG-2 Is Recruited to Endoplasmic Reticulum Exit Sites by Sec31A and Stabilizes the Localization of Sec31A Akinori Yamasaki, Katsuko Tani, Akitsugu Yamamoto, Naomi Kitamura and Masayuki Komada Mol. Biol. Cell November 1, 2006 vol. 17 no. 11 4876-4887
Control of Cortical Axon Elongation by a GABA-Driven Ca2+/Calmodulin-Dependent Protein Kinase Cascad Natsumi Ageta-Ishihara, Sayaka Takemoto-Kimura, Mio Nonaka, Aki Adachi-Morishima, Kanzo Suzuki, Satoshi Kamijo, Hajime Fujii, Tatsuo Mano, Frank Blaeser, Talal A. Chatila, Hidenobu Mizuno, Tomoo Hirano, Yoshiaki Tagawa, Hiroyuki Okuno and Haruhiko Bito The Journal of Neuroscience, October 28, 2009 29(43):13720 13729
Spatially asymmetric reorganization of inhibition establishes a motion-sensitive circuit Keisuke Yonehara, Kamill Balint, Masaharu Noda, Georg Nagel, Ernst Bamberg and Botond Roska Nature 469, 407-410 (20 January 2011)
Identification and developmental analysis of endothelin receptor type-A expressing cells in the mouse kidney Taro Kitazawa, Takahiro Sato, Koichi Nishiyama, Rieko Asai, Yuichiro Arima, Yasunobu Uchijima, Yukiko Kurihara, Hiroki Kurihara Gene Expr Patterns. 2011 Oct;11(7):371-7
Two Notch Ligands, Dll1 and Jag1, Are Differently Restricted in Their Range of Action to Control Neurogenesis in the Mammalian Spinal Cord Catarina Ramos, Susana Rocha, Claudia Gaspar, Domingos Henrique PLoS One. 2010 Nov 24;5(11):e15515
LKB1-Mediated Spatial Control of GSK3β and Adenomatous Polyposis Coli Contributes to Centrosomal Forward Movement and Neuronal Migration in the Developing Neocortex Naoyuki Asada and Kamon Sanada Journal of Neuroscience (2010)Volume: 30, Issue: 26, Pages: 8852-8865
Simple Motor System of the Ascidian Larva: Neuronal Complex Comprising Putative Cholinergic and GABAergic/Glycinergic Neurons Takeo Horie, Masashi Nakagawa,Yasunori Sasakura, Takehiro G. Kusakabe and Motoyuki Tsuda Zoological Science 27(2):181-190. 2010
Elimination of oncogenic neighbors by JNK-mediated engulfment in Drosophila Shizue Ohsawa, Kaoru Sugimura, Kyoko Takino, Tian Xu, Atsushi Miyawaki, Tatsushi Igaki Dev Cell. 2011 Mar 15; 20(3):315-28

for Western Blotting and Immunoblotting

Title Author Journal, Year Vol., Page
Lulu2 regulates the circumferential actomyosin tensile system in epithelial cells through p114RhoGEF Hiroyuki Nakajima and Takuji Tanoue J Cell Biol. 2011 October 17; 195(2): 245261
RUNX3 interactome reveals novel centrosomal targeting of RUNX family of transcription factors Linda Shyue Huey Chuang, Soak Kuan Lai, Maki Murata-Hori, Ayumi Yamada, Hoi-Yeung Li, Jayantha Gunaratne and Yoshiaki Ito Cell Cycle. 2012 May 15;11(10). Pages 1938-1947
ARHGAP18, a GTPase-activating protein for RhoA, controls cell shape, spreading, and motility Masao Maeda, Hitoki Hasegawa, Toshinori Hyodo, Satoko Ito, Eri Asano, Hong Yuang, Kohei Funasaka, Kaoru Shimokata, Yoshinori Hasegawa, Michinari Hamaguchi, and Takeshi Senga Mol Biol Cell. 2011 Oct;22(20):3840-52
Role of Palladin Phosphorylation by Extracellular Signal-Regulated Kinase in Cell Migration Eri Asano, Masao Maeda, Hitoki Hasegawa, Satoko Ito, Toshinori Hyodo, Hong Yuan, Masahide Takahashi, Michinari Hamaguchi, Takeshi Senga PloS one. 01/2011; 6(12):e29338
Novel BRCA2-interacting protein BJ-HCC-20A inhibits the induction of apoptosis in response to DNA damage Tomiyoshi G, Nakanishi A, Takenaka K, Yoshida K, Miki Y Cancer Sci. 2008 Apr;99(4):747-54
Enhanced Deacetylation of p53 by the Anti-apoptotic Protein HSCO in Association with Histone Deacetylase 1 Hisako Higashitsuji, Hiroaki Higashitsuji, Tomoko Masuda, Yu Liu, Katsuhiko Itoh, and Jun Fujita J Biol Chem. 2007 May 4;282(18):13716-25
ERK1/2 phosphorylate GEF-H1 to enhance its guanine nucleotide exchange activity toward RhoA Shuh-hei Fujishiro, Susumu Tanimura, Shogo Mure, Yuji Kashimoto, Kazushi Watanabe and Michiaki Kohno Biochemical and Biophysical Research Communications, 2008 Mar 28;368(1):162-7
Atg9A Protein, an Autophagy-related Membrane Protein, Is Localized in the Neurons of Mouse Brains Hirosumi Tamura, Masahiro Shibata, Masato Koike, Mitsuho Sasaki and Yasuo Uchiyama Histochem Cytochem. 2010 May; 58(5): 443453
Cooperative Role of Nectin-Nectin and Nectin-Afadin Interactions in Formation of Nectin-based Cell-Cell Adhesion Souichi Kurita, Hisakazu Ogita and Yoshimi Takai J Biol Chem. 2011 Oct 21;286(42):36297-303.
ALCAM (CD166) Is a Surface Marker for Early Murine Cardiomyocytes Hirokazu Hirata, Yoshinobu Murakami, Yoshiaki Miyamoto, Mako Tosaka, Kayoko Inoue, Ayako Nagahashi, Lars Martin Jakt, Takayuki Asahara, Hiroo Iwata, Yoshiki Sawa and Shin Kawamata Cells Tissues Organs 2006;184:172-180

Ordering Information

Product Name Application Storage Product No. PKG Size Price
Anti-GFP (Rat IgG2a), Monoclonal (GF090R) WB, IHC, ELISA R 04404-26 50 µg Inquire
04404-84 200 µg Inquire

(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze

Anti-GFP (Rat IgG2a), Mono (GF090R),CC, Agarose Conjugate

Applications

Western blotting

Samples: HEK-293 cell lysate expressing either EGFP or EGFPMyosin(1 ml each) Immunoprecipitation was performed with Anti-GFP agarose conjugate (20 µl) at 4°C for 2 hours.

Data courtesy of Dr. A. Kakizuka, Laboratory of Functional Biology, Graduate School of Biostudies, Kyoto University

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Epitope Tag Antibodies pdf (0.8 MB)

Ordering Information

Product Name Application Storage Product No. PKG Size Price
Anti-GFP (Rat IgG2a), Monoclonal(GF090R), CC, Agarose Conjugate IP R 06083-05 500 µg Inquire

(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze

Anti-GST (Mouse IgG2a-k), Mono (GS019)

Clone GS019
Isotype IgG2a-κ(mouse)
Product form Liquid
Immunogen Glutathione - S - Transferase(GST)
Application Western blotting 1:1000 - 1:2000
Immunoprecipitation 1:400 - 1:1000
ELISA 1:2000 - 1:20000

Applications

Sample:

  1. Lysate from E. coli expressioning GST protein
  2. Glutathione Sepharose flow-through fraction
  3. Glutathione Sepharose elution fraction
Gel 12.5% SDS-PAGE
Blocking Blocking One (Nacalai Tesque) /1 hr at RT
1st Antibody Anti-GST (Mouse IgG2a-κ) (1:2000 with Blocking One) /1 hr at RT
Wash TBS-T (5 min x 3)
2nd Antibody Anti-Mouse Ig-HRP (1:1000 with TBS-T)/30 min at RT
Wash TBS-T (5 min x 3)
Detection Luminol Reagent (Santa Cruz)

Data courtesy of Dr. Y. Shimizu, The Institute of Medical Science, The University of Tokyo

Downloads

Epitope Tag Antibodies pdf (0.8 MB)

References

for Western Blotting / Immunoblotting

Title Author Journal, Year Vol., Page
Lulu2 regulates the circumferential actomyosin tensile system in epithelial cells through p114RhoGEF Hiroyuki Nakajima and Takuji Tanoue J Cell Biol. 2011 October 17; 195(2): 245261
Protein Kinase C δ Regulates Ser46 Phosphorylation of p53 Tumor Suppressor in the Apoptotic Response to DNA Damage Kiyotsugu Yoshida, Hanshao Liu, and Yoshio Miki J Biol Chem. 2006 Mar 3;281(9):5734-40
DYRK2 Is Targeted to the Nucleus and Controls p53 via Ser46 Phosphorylation in the Apoptotic Response to DNA Damage Naoe Taira, Keishi Nihira, Tomoko Yamaguchi, Yoshio Miki, Kiyotsugu Yoshida Mol Cell. 2007 Mar 9;25(5):725-38Masatoshi Hara, Development 136 (10): 1687-1696
LOVASTATIN INSENSITIVE 1, a Novel Pentatricopeptide Repeat Protein, is a Potential Regulatory Factor of Isoprenoid Biosynthesis in Arabidopsis Keiko Kobayashi, Masashi Suzuki, Jianwei Tang, Noriko Nagata, Kiyoshi Ohyama, Hikaru Seki, Reiko Kiuchi, Yasuko Kaneko, Miki Nakazawa, Minami Matsui, Shogo Matsumoto, Shigeo Yoshida and Toshiya Muranaka Plant Cell Physiol. 48(2): 322331 (2007)
Regulation of melanin-concentrating hormone receptor 1 signaling by RGS8 with the receptor third intracellular loop Mayumi Miyamoto-Matsubara, Osamu Saitoh, Kei Maruyama, Yoshimi Aizaki, Yumiko Saito Cell Signal. 2008 Nov;20(11):2084-94
Molecular diversity of the two sugar-binding sites of the β-trefoil lectin HA33/C (HA1) from Clostridium botulinum type C neurotoxin Toshio Nakamura, Takashi Tonozuka, Sakae Ito, Yoichi Takeda, Ryutaro Sato, Ichiro Matsuo, Yukishige Ito, Keiji Oguma, Atsushi Nishikawa Arch Biochem Biophys. 2011 Aug 1;512(1):69-77
Tyrosine Phosphorylation of 3BP2 Regulates B Cell Receptor-mediated Activation of NFAT Upasana Shukla, Tomoko Hatan, Kenji Nakashima, Kazuhiro Ogi and Kiyonao Sada J Biol Chem. 2009 December 4; 284(49): 3371933728
ATM Augments Nuclear Stabilization of DYRK2 by Inhibiting MDM2 in the Apoptotic Response to DNA Damage Naoe Taira, Hiroyuki Yamamoto, Tomoko Yamaguchi, Yoshio Miki and Kiyotsugu Yoshida J Biol Chem. 2010 February 12; 285(7): 49094919
Leukotriene B4 Augments and Restores Fc γRs-dependent Phagocytosis in Macrophages Fuyuki Okamoto, Kazuko Saek, Hideki Sumimoto, Sho Yamasaki and Takehiko Yokomizo J Biol Chem. 2010 December 24; 285(52): 4111341121
The yeast VAP homolog Scs2p has a phosphoinositide-binding ability that is correlated with its activity Satoshi Kagiwada and Misa Hashimoto Biochemical and Biophysical Research Communications. 2007 Dec 28;364(4):870-6
Intracellular protein delivery activity of peptides derived from insulin-like growth factor binding proteins 3 and 5 Natsuko Goda, Takeshi Tenno, Kosuke Inomata, Masahiro Shirakawa, Toshiki Tanaka, Hidekazu Hiroaki Exp Cell Res. 2008 Aug 1;314(13):2352-61
The common phospholipid-binding activity of the N-terminal domains of PEX1 and VCP/p97 Kumiko Shiozawa, Natsuko Goda, Toshiyuki Shimizu, Kenji Mizuguch, Naomi Kondo, Nobuyuki Shimozawa, Masahiro Shirakawa and Hidekazu Hiroaki FEBS J. 2006 Nov;273(21):4959-71
Novel BRCA2-interacting protein BJ-HCC-20A inhibits the induction of apoptosis in response to DNA damage Go Tomiyoshi, Akira Nakanishi, Katsuya Takenaka, Kiyotsugu Yoshida and Yoshio Miki Cancer Sci. 2008 Apr;99(4):747-54
Human protein factory for converting the transcriptome into an in vitroexpressed proteome Naoki Goshima, Yoshifumi Kawamura, Akiko Fukumoto, Aya Miura, Reiko Honma, Ryohei Satoh, Ai Wakamatsu, Jun-ichi Yamamoto, Kouichi Kimura, Tetsuo Nishikawa, Taichi Andoh, Yuki Iida, Kumiko Ishikawa, Emi Ito, Naoko Kagawa, Chie Kaminaga, Kei-ichi Kanehori, Bunsei Kawakami, Kiyokazu Kenmochi, Rie Kimura, Miki Kobayashi, Toshihiro Kuroita, Hisashi Kuwayama, Yukio Maruyama, Kiyoshi Matsuo, Kazuyoshi Minami, Mariko Mitsubori, Masatoshi Mori, Riyo Morishita, Atsushi Murase, Akira Nishikawa, Shigemichi Nishikawa, Toshihiko Okamoto, Noriko Sakagami, Yutaka Sakamoto, Yukari Sasaki, Tomoe Seki, Saki Sono, Akio Sugiyama, Tsuyoshi Sumiya, Tomoko Takayama, Yukiko Takayama, Hiroyuki Takeda, Takushi Togashi, Kazuhide Yahata, Hiroko Yamada, Yuka Yanagisawa, Yaeta Endo, Fumio Imamoto, Yasutomo Kisu, Shigeo Tanaka, Takao Isogai, Jun-ichi Imai, Shinya Watanabe and Nobuo Nomura Nature Methods 5, 1011 - 1017 (2008)
Pim-1 controls NF-κB signalling by stabilizing RelA/p65 K Nihira, Y Ando, T Yamaguchi, Y Kagami, Y Miki and K Yoshida Cell Death and Differentiation (2010) 17, 689698

Ordering Information

Product Name Application Storage Product No. PKG Size Price
Anti-GST (Mouse IgG2a-κ), Monoclonal (GS019) WB, IP
ELISA
R 04435-84 50µg Inquire
04435-26 200µg Inquire

(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze

Anti-GST, POD Conjugated

Clone GS019
Isotype IgG2a-κ(mouse)
Product form Liquid
&Immunogen Glutathione-s-Transferase (GST)
Application Western blotting 1:4000 - 1:8000
ELISA 1:30000 - 1:60000

Downloads

Epitope Tag Antibodies pdf (0.8 MB)

References

for Immunoblotting

Title Author Journal, Year Vol., Page
Glycoside Hydrolase Family 89α-N-acetylglucosaminidase from Clostridium perfringens Specifically Acts on GlcNAc 1,4Galβ1R at the Non-reducing Terminus of O-Glycans in Gastric Mucin Masaya Fujita, Akiko Tsuchida, Akiko Hirata, Natsumi Kobayash, Kohtaro Goto, Kenji Osumi, Yuriko Hirose, Jun Nakayama, Takashi Yamano, Hisashi Ashida and Mamoru Mizuno J Biol Chem. 2011 February 25; 286(8): 64796489

Ordering Information

Product Name Application Storage Product No. PKG Size Price
Anti-GST (Mouse IgG2a-κ), Monoclonal (GS019), AS, POD Conjugated WB, ELISA R 04559-74 50µg Inquire

(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze

Anti-HA (Mouse IgG1-κ), Mono (HA124)

Clone HA124
GF090R IgG1-k (Mouse)
Product form Liquid
Immunogen Gulutathione-S-Transferase (GST)
-HA[YPYDVPDYA-COOH] fusion protein
Application Western Blotting 1:10000-1:30000
ELISA 1:2000-1:20000

Applications

Western blotting

Sample

  1. E. coli lysate
  2. NH2-(GST)(T7)(HSV)(c-Myc)(VSV)
    (Glu-Glu)(V5)(E-tag)(DDDDK)(S-tag)(HA)
    (6xHis)-COOHNH (GST) (abcam, #ab5395) 250 ng
  3. NH2-(GST)(HA)-COOH (PIERCE, #23613) 20 ng
  4. NH2-(GST)(HA)-COOH (PIERCE, #23613) 100 ng
  5. M.W. marker
1st antibody Anti-HA (Mouse IgG1-κ), Mono (HA124),
1:10000, 60min
2nd antibody Anti-Mouse IgG(Rat IgG), CC, POD Conjugated 1:25000, 60min
Detection Chemi-Lumi One (Product No. 05027-20), 1 min

Immunoprecipitation and gel staining

  1. Clarified bacteria lysate
    +HA-tagged GST 1 µg
    +Anti-HA (Mouse IgG1-κ), Mono (HA124), 5 µg
    +Protein G Agarose (50% Suspention) 0.05 ml
  2. E coli lysate
    +NH2-(GST)(HA)-COOH(PIERECE, #23613) 1 µg

Detection : Silver staining

Downloads

Epitope Tag Antibodies pdf (0.8 MB)

References

for Immunoblotting

Title Author Journal, Year Vol., Page
Interaction between Periostin and BMP-1 Promotes Proteolytic Activation of Lysyl Oxidase Takumi Maruhashi, Isao Kii, Mitsuru Saito and Akira Kudo THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 285, NO. 17, pp. 13294-13303
A novel vanadium transporter of the Nramp family expressed at the vacuole of vanadium-accumulating cells of the ascidian Ascidia sydneiensis samea Tatsuya Ueki, Nobuaki Furuno and Hitoshi Michibata Biochim Biophys Acta. 2011 Apr;1810(4):457-64
Intra-axonal Patterning: Intrinsic Compartmentalization of the Axonal Membrane in Drosophila Neuron Takeo Katsuki, Deepak Ailani, Masaki Hiramoto and Yasushi Hiromi Neuron. 2009 Oct 29;64(2):188-99
Red1 promotes the elimination of meiosis-specific mRNAs in vegetatively growing fission yeast Tomoyasu Sugiyama and Rie Sugioka-Sugiyama The EMBO Journal (2011) 30, 1027-1039

Ordering Information

Product Name Application Storage Product No. PKG Size Price
Anti-HA (Mouse IgG1-k), Monoclonal (HA124) WB, ELISA R 06340-96 50 µg Inquire
06340-54 200 µg Inquire

(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze

Anti-6xHis (Mouse IgG1a-k), Mono (HI192)

Clone HI192
GF090R IgG1-k (Mouse)
Product form Liquid
Immunogen 6xHis synthetic peptide [HHHHHH] conjugated with KLH
Application Western Blotting 1:1000-1:2000
ELISA 1:2000-1:20000

Applications

Western Blotting

Sample:
Lysate from Sf9 cells in which His-tagged Drosophila PTEN is expressed

Filter : FluoroTrans [PALL]
Blocking : 5% non-fat dry milk/PBS (30 min)
1st antibody : Anti-6xHis (Mouse IgG1-), Monoclonal (HI192)
Wash : 0.25% Tween-20/TBS (10 min x 3)
2nd antibody : Anti-Mouse IgG-HRP (1:1000) /2% BSA /0.25% Tween-20 /TBS (30 min)
Wash :0.25% Tween-20/TBS (10 min x 5)
Detection : Luminol Reagent (Santa Cruz)

Data courtesy of Dr. A. Maehama, The Tokyo Metropolitan Institute of Medical Science (RINSHOKEN)

Downloads

Epitope Tag Antibodies pdf (0.8 MB)

References

for Immunoprecipitation

Title Author Journal, Year Vol., Page
Reduction of transforming growth factor-βtype II receptor is caused by the enhanced ubiquitin-dependent degradation in human renal cell carcinoma Hirotaka Fukasawa, Tatsuo Yamamoto, Yoshihide Fujigaki, Taro Misaki, Naro Ohashi, Tatsuya Takayama, Sayuri Suzuki, Soichi Mugiya, Toshiaki Oda, Chiharu Uchida, Kyoko Kitagawa, Takayuki Hattori, Hidetoshi Hayashi, Seiichiro Ozono, Masatoshi Kitagawa and Akira Hishida Int J Cancer. 2010 Oct 1;127(7):1517-25

Ordering Information

Product Name Application Storage Product No. PKG Size Price
Anti-6xHis (Mouse IgG1-k), Monoclonal (HI192) WB, ELISA R 04428-26 50 µg Inquire
04428-84 200 µg Inquire

(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze

Anti-6xHis, POD Conjugated

Clone HI192
Isotype IgG1a-Κ (mouse)
Product form Liquid
Immunogen 6xHis synthetic peptide [HHHHHH] conjugated with KLH
Application Western blot ting 1:1000 - 1:2000
ELISA 1:30000 - 1:60000

Each concentration of GFP-His6 protein synthesized by in vitro translation were apllied 2.5 μl to gel.

Applications

Western blotting

Lane 1 Sample: GFP-His6, undiluted solution
1st Antibody: Anti-His6 produced by A company, 1:100,00
Lane 2 Sample: GFP-His6, 10-fold dilution
1st Antibody: Anti-His6 produced by A company, 1:100,000
Lane 3 Sample: GFP-His6, undiluted solution
1st Antibody: Anti-6xHis, POD conjugated (04546-34), 1:100,000
Lane 4 Sample: GFP-His6, 10-fold dilution
1st Antibody: Anti-6xHis, POD conjugated (04546-34), 1:100,000

Downloads

Epitope Tag Antibodies pdf (0.8 MB)

References

for Western Blotting / Immunoblotting

Title Author Journal, Year Vol., Page
Reduction of transforming growth factor-β type II receptor is caused by the enhanced ubiquitin-dependent degradation in human renal cell carcinoma Hirotaka Fukasawa, Tatsuo Yamamoto, Yoshihide Fujigaki, Taro Misaki, Naro Ohashi, Tatsuya Takayama, Sayuri Suzuki, Soichi Mugiya, Toshiaki Oda, Chiharu Uchida, Kyoko Kitagawa, Takayuki Hattori, Hidetoshi Hayashi, Seiichiro Ozono, Masatoshi Kitagawa and Akira Hishida Int J Cancer. 2010 Oct 1;127(7):1517-25
Numb controls E-cadherin endocytosis through p120 catenin with aPKC Kazuhide Sato, Takashi Watanabe, Shujie Wang, Mai Kakeno, Kenji Matsuzawa, Toshinori Matsui, Keiko Yokoi, Kiyoko Murase, Ikuko Sugiyama, Masayuki Ozawa and Kozo Kaibuchi Mol. Biol. Cell September 1, 2011 vol. 22 no. 17 3103-3119
Longistatin, a novel EF-hand protein from the ixodid tick Haemaphysalis longicornis, is required for acquisition of host blood-meals Anisuzzaman, M. Khyrul Islam, Takeharu Miyoshi, M. Abdul Alim, Takeshi Hatta, Kayoko Yamaji, Yasunobu Matsumoto, Kozo Fujisaki and Naotoshi Tsuji Int J Parasitol. 2010 May;40(6):721-9
Protein Crosslinking by Transglutaminase Controls Cuticle Morphogenesis in Drosophila Toshio Shibata, Shigeru Ariki, Naoaki Shinzawa, Ryuta Miyaji, Haruka Suyama, Miyuki Sako, Nobuyuki Inomata, Takumi Koshiba, Hirotaka Kanuka and Shun-ichiro Kawabata PLoS One. 2010 Oct 18;5(10):e13477
Genetic Modification of Bacillus subtilis for Production of D-chiro-Inositol, an Investigational Drug Candidate for Treatment of Type 2 Diabetes and Polycystic Ovary Syndrome Ken-ichi Yoshida, Masanori Yamaguchi, Tetsuro Morinaga, Maya Ikeuchi, Masaki Kinehara and Hitoshi Ashida Appl Environ Microbiol. 2006 February; 72(2): 13101315
Coupled cytoplasmic transcription-and-translation-a method of choice for heterologous gene expression in Xenopus oocytes Alexander A. Tokmakov, Eiko Matsumoto, Mikako Shirouzu and Shigeyuki Yokoyama J Biotechnol. 2006 Mar 9;122(1):5-15
Tandem multimer expression of angiotensin I-converting enzyme inhibitory peptide in Escherichia coli Fida, Hasan M; Kumada, Yoichi; Terashima, Masaaki; Katsuda, Tomohisa; Katoh, Shigeo Biotechnol J. 2009 Sep;4(9):1345-56
myo-Inositol Catabolism in Bacillus subtilis Ken-ichi Yoshida, Masanori Yamaguchi, Tetsuro Morinaga, Masaki Kinehara, Maya Ikeuchi, Hitoshi Ashida and Yasutaro Fujita The Journal of Biological Chemistry, 283, 10415-10424
Activation of T7 RNA polymerase in Xenopus oocytes and cell-free extracts Alexander A. Tokmakov and Yasuo Fukami Genes Cells. 2010 Nov;15(11):1136-44
Molecular cloning and characterization of Nop25, a novel nucleolar RNA binding protein, highly conserved in vertebrate species Shunji Suzuki, Motoko Kanno, Takashi Fujiwara, Hironobu Sugiyama, Akiko Yokoyama, Hisaaki Takahashi and Junya Tanaka Exp Cell Res. 2006 Apr 15;312(7):1031-41
A novel GTPase, CRAG, mediates promyelocytic leukemia protein-associated nuclear body formation and degradation of expanded polyglutamine protein Qingyu Qin, Ryoko Inatome, Azusa Hotta, Masaki Kojima, Hirohei Yamamura and Hirokazu Hirai J Cell Bio. 2006 Feb 13:172(4):497-504

Ordering Information

Product Name Application Storage Product No. PKG Size Price
Anti-6xHis (Mouse IgG1-Κ), Monoclonal (HI192), AS, POD Conjugated WB, ELISA R 04546-34 50 µg Inquire

(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze

Anti-DYKDDDDK(Mouse IgG2b-κ), Mono

Clone 5A8E5
Isotype IgG2b-κ (Mouse)
Product form Liquid
Specificity Anti-DYKDDDDK recognizes C-terminal, N-terminal and internal tagged fusion proteins
Concentration 0.5 mg/ml, lyophilized with PBS, pH7.4, containing 0.02% sodium azide.
Immunogen A synthetic peptide (DYKDDDDK) coupled KLH
Application Western Blotting 0.1-1.0 µg/ml
Immunoprecipitation 1 µg/ml
Immunofluorescent 1 µg/ml
ELISA 0.05-0.2 µg/ml

Applications

Western blotting

Sample Lane 1. N-terminal DYKDDDDK-tagged fusion protein
Lane 2. Internal DYKDDDDK-tagged fusion protein
Lane 3. C-terminal DYKDDDDK-tagged fusion protein
1st Antibody A. Anti-DYKDDDDK(Mouse IgG2b-κ), Mono (5A8E5) 1 mg/ml
B. Company S, Anti-DYKDDDDK Tag mAb (M2) 1 mg/ml
2nd Antibody Goat anti-Mouse IgG IRDyeTM 800 Conjugated

Western blotting

Sample Lane 1-3. Multiple Tag (Purified) (400 ng, 80 ng, 16 ng)
Lane 4. 293 cell lysate 10 ml
1st antibody A. Anti-DYKDDDDK(Mouse IgG2b-κ), Mono (5A8E5) 1 mg/ml
B. Company S, Anti-DYKDDDDK Tag mAb (M2) 1 mg/ml
2nd antibody Goat anti-Mouse IgG IRDyeTM 800 Conjugated

Downloads

Epitope Tag Antibodies pdf (0.8 MB)

Ordering Information

Product Name Application Storage Product No. PKG Size Price
Anti-DYKDDDDK (Mouse IgG2b-κ), Mono WB, IP
IF, ELISA
R NU01102 200 µg Inquire

(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze

Anti-DYKDDDDK (Mouse), Monoclonal Agarose Conjugate

Applications

Western Blotting

App of WB

Lane 1. DYKDDDDK tagged fusion protein input control
Lane 2. Anti-DYKDDDDK, Agarose Conjugated
Lane 3. DYKDDDDK tagged fusion protein was precipitated by
Anti-DYKDDDDK, agarose conjugated.

Clone 6H8E4
Degree of substitution Approx. 8.5 mg Anti-DYKDDDDK mAb/ml settled resin
Capacity > 1 mg/ml settled resin
Product form 1x PBS containing 50% glycerol

Downloads

Epitope Tag Antibodies pdf (0.8 MB)

Ordering Information

Product Name Application Storage Product No. PKG Size Price
Anti-DYKDDDDK (Mouse), Monoclonal Agarose Conjugate IP R NU01103 1 ml Inquire

(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze

Anti-V5 (Mouse IgG1-κ), Mono (V5005)

Clone V5005
Isotype IgG1-κ(mouse)
Product form Liquid
Immunogen V5 synthetic peptide [GKPIPNPLLGLDST] conjugated with KLH
Application Western blotting 1:1000 - 1:2000
Immunoprecipitation 1:400 - 1:1000
ELISA 1:2000 - 1:20000

Applications

Western blotting, Immunoprecipitation

(A) Western blotting VE-Card.-V5
Sample Lysate from 293T cell in which V5-tagged (C-terminal) pCDNA dest40-VE-cadherin is expressed
Antibody Anti-V5 (Mouse IgG1-κ) (1:5000)

(B) Immunoprecipitation VE-Card. -V5
Sample Lysate (A)
Antibody Anti-V5 (Mouse IgG1-κ) (1μl)
Western blotting Anti-V5 (Mouse IgG1-κ) (1:2000)

Downloads

Epitope Tag Antibodies pdf (0.8 MB)

References

for Immunostaining

Title Author Journal, Year Vol., Page
Activation of the small GTPase Rac1 by a specific guanine-nucleotide-exchange factor suffices to induce glucose uptake into skeletal-muscle cells Shuji Ueda, Tohru Kataoka and Takaya Satoh Biol. Cell (2008) 100, 645657
Oxidized LDL Receptor LOX-1 Binds to C-Reactive Protein and Mediates Its Vascular Effects Yoshiko Fujita, Akemi Kakino, Norihisa Nishimichi, Saburo Yamaguchi, Yuko Sato, Sachiko Machida, Luciano Cominacini, Yves Delneste, Haruo Matsuda and Tatsuya Sawamura Clinical Chemistry 55:2 285294 (2009)
C-Reactive Protein Uptake by Macrophage Cell Line via Class-A Scavenger Receptor Yoshiko Fujita, Akemi Kakino, Mariko Harada-Shiba, Yuko Sato, Kazunori Otsui, Ryo Yoshimoto and Tatsuya Sawamura Clinical Chemistry 56:3 478481 (2010)
A novel mutation of ALK2, L196P, found in the most benign case of fibrodysplasia ossificans progressiva activates BMP-specific intracellular signaling equivalent to a typical mutation, R206H Satoshi Ohte, Masashi Shin, Hiroki Sasanuma, Katsumi Yoneyama, Masumi Akita, Kenji Ikebuchi, Eijiro Jimi, Yuichi Maruki, Masaru Matsuoka, Akira Namba, Hiroshi Tomoda, Yasushi Okazaki, Akira Ohtake, Hiromi Oda, Ichiro Owan, Tetsuya Yoda, Hirokazu Furuya, Jyunji Kamizono, Hiroshi Kitoh, Yasuharu Nakashima, Takafumi Susami, Nobuhiko Haga, Tetsuo Komori and Takenobu Katagiri Biochem Biophys Res Commun. 2011 Apr 1;407(1):213-8

for Immunoprecipitation

Title Author Journal, Year Vol., Page
Phosphorylation-Dependent Protein Interaction with Trypanosoma brucei 14-3-3 Proteins that Display Atypical Target Recognition Masahiro Inoue, Kouichi Yasuda, Haruki Uemura, Natsumi Yasaka, Hiroshi Inoue, Yoshitatsu Sei, Nobuo Horikoshi, Toshihide Fukuma PLoS One. 2010 Dec 21;5(12):e15566

for Western Blotting / ELISA

Title Author Journal, Year Vol., Page
Interaction between PKR and PACT mediated by LPS-inducible NF-κB in human gingival cells Kaya Yoshida, Hirohiko Okamura, Yumi Hoshino, Masayuki Shono, Masami Yoshioka, Daisuke Hinode and Hideo Yoshida Journal of Cellular Biochemistry 113:165173 (2012)
Calcium-dependent platelet aggregation through the interaction between phosphatidylserine and LOX-1 Kazunori Otsui, Makoto Kakutani, Koji Yuasa, Atushi Nakano, Chang-Ping Hu, Akemi Kakino, Yuko Sato, Yoshiko Fujita, Yosuke Kawase, Kou-ichi Jishage, Hiroshi Suzuki, Jawahar L. Mehta, Mitsuhiro Yokoyama, Ken-ichi Hirata, Nobutaka Inoue, and Tatsuya Sawamura Cardiovasc Res. 2010 Dec 1;88(3):547
The orphan nuclear receptor GCNF recruits DNA methyltransferase for Oct-3/4 silencing Noriko Sato, Mitsumasa Kondo and Ken-ichi Arai Biochemical and Biophysical Research Communications 344 (2006) 845851

Ordering Information

Product Name Application Storage Product No. PKG Size Price
Anti-V5 (Mouse IgG1-k), Monoclonal (V5005) WB, IP
ELISA
R 04434-94 50 µg Inquire
04434-36 200 µg Inquire

(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze

Anti-V5, POD Conjugated

Clone GF200
Isotype IgG1-Κ (mouse)
Product form Liquid
Immunogen V5 synthetic peptide [GKPIPNPLLGLDST] conjugated with KLH
Application Western blotting 1:1000 - 1:2000
ELISA 1:8000 - 1:16000

References

for Immunoblotting

Title Author Journal, Year Vol., Page
Physical and Functional Interaction of Transmembrane Thioredoxin-related Protein with Major Histocompatibility Complex Class I Heavy Chain: Redox-based Protein Quality Control and Its Potential Relevance to Immune Responses Yoshiyuki Matsuo, Hiroshi Masutani, Aoi Son, Shinae Kizaka-Kondoh and Junji Yodoi Molecular Biology of the Cell, Vol. 20, 45524562

Ordering Information

Product Name Application Storage Product No. PKG Size Price
Anti-V5 (Mouse IgG1-κ), Monoclonal (V5005), AS, POD Conjugated WB, ELISA R 04578-24 50 µg Inquire

(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze