NACALAI TESQUE, INC.

Compositions of each product are available on online catalog, “e-Nacalai Search Version”.

Ordering information

Product name	Storage	Product No.	PKG Size
DMEM(1.0g/l Glucose) with L-Gln and Sodium Pyruvate, liquid	R	08456-65	500 ml
DMEM(1.0g/l Glucose) with Sodium Pyruvate, without L-Gln and Phenol Red, liquid	R	08490-05	500 ml
DMEM(4.5g/l Glucose) with L-Gln and HEPES, without Sodium Pyruvate, liquid	R	08457-55	500 ml
DMEM(4.5g/l Glucose) with L-Gln and Sodium Pyruvate, liquid	R	08458-45	500 ml
DMEM(4.5g/l Glucose) with L-Gln, without Sodium Pyruvate, liquid	R	08459-35	500 ml
DMEM(4.5g/l Glucose) without L-Gln, Sodium Pyruvate and Phenol Red, liquid	R	08489-45	500 ml
DMEM(4.5g/l Glucose) without L-Gln and Sodium Pyruvate, liquid	R	08488-55	500 ml
DMEM (No Glucose) with L-Gln, without Sodium Pyruvate, liquid	R	09891-25	500 ml
DMEM/Ham's F-12 with L-Gln, Sodium Pyruvate and HEPES, liquid	R	08460-95	500 ml
DMEM/Ham's F-12 with L-Gln, Sodium Pyruvate and HEPES, liquid without Phenol Red, liquid	R	05177-15	500 ml
DMEM/Ham's F-12 (No Glucose) with L-Gln and Sodium Pyruvate, liquid	R	09893-05	500 ml
Ham's F-12 with L-Gln, liquid	R	17458-65	500 ml
MEM with Earle's Salts and L-Gln, liquid	R	21442-25	500 ml
MEM with Earle's Salts, L-Gln and Non-Essential Amino Acids, liquid	R	21443-15	500 ml
MEM (No Glucose) with Earle's Salts, L-Gln and Non-Essential Amino Acids, liquid	R	09848-05	500 ml
α-MEM with L-Gln, Ribonucleosides and Deoxyribonucleosides, liquid	R	21444-05	500 ml
α-MEM with L-Gln, without Ribonucleosides and Deoxyribonucleosides, liquid	R	21445-95	500 ml
RPMI 1640 with L-Gln and HEPES, liquid	R	30263-95	500 ml
RPMI 1640 with L-Gln, liquid	R	30264-85	500 ml
RPMI 1640 with L-Gln, without Phenol Red, liquid	R	06261-65	500 ml
RPMI 1640 with L-Gln, liquid	R	05176-25	500 ml
RPMI 1640 (No Glucose) with L-Gln, liquid	R	09892-15	500 ml

(Storage) R = Refrigerator

Balanced Salines

D-PBS(+) Preparation Reagent (Ca,Mg Solution)(100x)

• Easy to prepare D-PBS(+)
• Reduce phosphate precipitation to prepare as needed
• Sterile prepared solution
• Endtoxin and mycoplasma free (Can use for washing cell in culture)

Ordering information

Product name	Storage	Product No.	PKG Size
D-PBS(+) Preparation Reagent (Ca,Mg Solution) (100x)	RT	02492-94	30 ml
D-PBS(-) without Ca and Mg, liquid	RT	14249-95	500 ml
D-PBS(-) without Ca and Mg, Powder	RT	07269-84	100 g
HBSS(+) with Ca, Mg and Phenol Red, liquid	RT	17459-55	500 ml
HBSS(+) with Ca, Mg, without Phenol Red, liquid	RT	09735-75	500 ml
HBSS(-) without Ca and Mg, with Phenol Red, liquid	RT	17460-15	500 ml
HBSS(-) without Ca, Mg and Phenol Red, liquid	RT	17461-05	500 ml

Supplements

• Sterilized by membrane filter (pore size 0.2 µm)
• Sterility tested (for Bacteria, Fungus and Mycoplasma)
• Endotoxin tested

MEM Non-Essential Amino Acids Solution (100x)

Composition: Non-Essential Amino Acids 10 mM each

L-Alanine	890
L-Asparagine,H2O	1,500
L-Asparatic Acid	1,330
L-Glutamic Acid	1,470
Glycine	750
L-Proline	1,150
L-Serine	1,050
Non-Essential Amino Acids	(mg/ml)

100mM-Sodium Pyruvate Solution (100x)

Pyruvic Acid Solution Culture Test

Ordering information

Product name	Storage	Product No.	PKG Size
L-Alanyl-L-glutamine	R	01102-82	25 g
200mmol/l L‐Alanyl‐L‐glutamine Solution(100x)	F	04260-64	100 ml
200mM‐L‐Glutamine Stock Solution	F	16948-04	100 ml
200mM-L-Glutamine Stock Solution	F	16948-04	100 ml
1mol/l-HEPES Buffer Solution	R	17557-94	100 ml
MEM Non-Essential Amino Acids Solution(100x)	R	06344-14	20 ml
		06344-56	100 ml
100mM-Sodium Pyruvate Solution(100x)	R	06977-34	100 ml
apo-Transferrin from Human	R	34401-84	100 mg
		34401-55	500 mg

(Storage) R = Refrigerator, F = Freezer

Cell Dissociation Reagents

Ordering information

Product name	Storage	Product No.	PKG Size
2.5g/l-Trypsin Solution	F	35555-54	100 ml
5.0g/l-Trypsin/5.3mmol/l-EDTA Solution	F	35556-44	100 ml
2.5g/l-Trypsin/1mmol/l-EDTA Solution	F	35554-64	100 ml
2.5g/l-Trypsin/1mmol/l-EDTA Solution, with Phenol Red	F	32777-44	100 ml
0.5g/l-Trypsin/0.53mmol/l-EDTA Solution	F	35553-74	100 ml
0.5g/l-Trypsin/0.53mmol/l-EDTA Solution, with Phenol Red	F	32778-34	100 ml
0.2g/l-EDTA Solution	R	14367-74	100 ml

(Storage) R = Refrigerator, F = Freezer

Antibiotics

Ordering information

Product name	Application	Storage	Product No.	PKG Size
Actinomycin D Solution(1mg/ml)	Other Antibiotics	F	00393-41	1 ml
Antibiotic-Antimycotic Mixed Stock Solution(100x)	Bacteria, Fungal, Yeast	F	02892-54	100 ml
Antibiotic-Antimycotic Mixed Stock Solution(100x)(Stabilized)	Bacteria, Fungal, Yeast	F	09366-44	100 ml
Colcemid Solution(10µg/ml)	Other Antibiotics	R	09356-74	10 ml
G 418 Disulfate	Selection Antibiotics	RT	16512-36	250 mg
			16512-81	1 g
			16512-94	5 g
			16512-52	25 g
G 418 Disulfate	Other Antibiotics	RT	08973-01	1 g
			08973-14	5 g
G 418 Disulfate Aqueous Solution	Selection Antibiotics	R	09380-86	20 ml
			09380-44	100 ml
Gentamicin Sulfate	Bacteria/Mycoplasma	R	08975-81	1 g
			08975-94	5 g
Gentamicin Sulfate Solution (10mg/ml)	Bacteria/Mycoplasma	R	16672-04	10 ml
Hygromycin B	Selection Antibiotics	R	07296-66	100 mg
			07296-11	1 g
Hygromycin B Solution	Selection Antibiotics	R	09287-84	20 ml
Kanamycin Monosulfate	Selection Antibiotics	RT	08976-71	1 g
			08976-84	5 g
Mitomycin C Solution(1mg/ml)	Other Antibiotics	F	20898-21	1 ml
Penicillin-Streptomycin Mixed Solution Penicillin 10,000u/ml, Streptomycin 10,000µg/ml	Bacteria(Gram-positive bacteria/ Gram-negative bacteria)	F	26253-84	100 ml
Penicillin-Streptomycin-Glutamine Mixed Solution Penicillin 10,000u/ml, Streptomycin 10,000µg/ml, L-Glutamine 29.2mg/ml, Sodium Chloride 0.14%, Citrate Buffer Solution 10mM	Bacteria(Gram-positive bacteria/ Gram-negative bacteria)	F	06168-34	100 ml
Penicillin-Streptomycin Mixed Solution (Stabilized) Penicillin 10,000u/ml, Streptomycin 10,000µg/ml	Bacteria(Gram-positive bacteria/ Gram-negative bacteria)	F	09367-34	100 ml
Penicillin-Streptomycin Mixed Solution Penicillin 5,000u/ml, Streptomycin 5,000µg/ml	Bacteria(Gram-positive bacteria/ Gram-negative bacteria)	F	26252-94	100 ml
Streptomycin Sulfate	Gram-negative bacteria	R	32204-34	5 g
			32204-92	25 g

(Storage) RT = Room Temperature, R = Refrigerator, F = Freezer