Cell Culture Reagents

Features

  • Animal origin-free
  • Sterility tested for bacteria, fungus and mycoplasma
  • Endotoxin tested
  • pH 7.1-7.5
Product Images

Cell Culture Medium

Compositions

Compositions

Compositions of each product are available on online catalog, “e-Nacalai Search Version”.

Downloads

Cell Culture Medium(PDF 6 MB)

▲Page top

Ordering information

Product name Storage Product No. PKG Size Online Catalog
DMEM (1.0g/l Glucose) with L-Gln and Sodium Pyruvate, liquid R 08456-65 500 ml e-Nacalai  
08456-36 10 bottles x 500 ml
DMEM (1.0g/l Glucose) with Sodium Pyruvate, without L-Gln and Phenol Red, liquid R 08490-05 500 ml
DMEM (4.5g/l Glucose) with L-Gln and HEPES, without Sodium Pyruvate, liquid R 08457-55 500 ml
DMEM (4.5g/l Glucose) with L-Gln and Sodium Pyruvate, liquid R 08458-45 500 ml
08458-16 10 bottles x 500 ml
DMEM (4.5g/l Glucose) with L-Gln, without Sodium Pyruvate, liquid R 08459-35 500 ml
08459-64 10 bottles x 500 ml
DMEM (4.5g/l Glucose) without L-Gln, Sodium Pyruvate and Phenol Red, liquid R 08489-45 500 ml
DMEM (4.5g/l Glucose) without L-Gln and Sodium Pyruvate, liquid R 08488-55 500 ml
DMEM (4.5g/l Glucose) with Sodium Pyruvate, without L-Gln, liquid R 11584-85 500 ml
DMEM (4.5g/l Glucose) with HEPES, without L-Gln and Sodium Pyruvate, liquid R 11585-75 500 ml
DMEM (No Glucose) with L-Gln, without Sodium Pyruvate, liquid R 09891-25 500 ml
DMEM/Ham's F-12 with L-Gln, Sodium Pyruvate and HEPES, liquid R 08460-95 500 ml
DMEM/Ham's F-12 with L-Gln, Sodium Pyruvate and HEPES, without Phenol Red, liquid R 05177-15 500 ml
DMEM/Ham's F-12 with L-Gln and Sodium Pyruvate, without HEPES, liquid R 11581-15 500 ml
DMEM/Ham's F-12 with L-Gln and Sodium Pyruvate, without HEPES and Phenol Red, liquid R 11582-05 500 ml
DMEM/Ham's F-12 with Sodium Pyruvate and HEPES, without L-Gln, liquid R 11583-95 500 ml
DMEM/Ham's F-12 (No Glucose) with L-Gln and Sodium Pyruvate, liquid R 09893-05 500 ml
IMDM with L-Gln and HEPES, liquid (Iscove's Modified Dulbecco's Medium) R 11506-05 500 ml
Ham's F-12 with L-Gln, liquid R 17458-65 500 ml
MEM with Earle's Salts and L-Gln, liquid R 21442-25 500 ml
MEM with Earle's Salts, L-Gln and Non-Essential Amino Acids, liquid R 21443-15 500 ml
MEM (No Glucose) with Earle's Salts, L-Gln and Non-Essential Amino Acids, liquid R 09848-05 500 ml
α-MEM with L-Gln, Ribonucleosides and Deoxyribonucleosides, liquid R 21444-05 500 ml
α-MEM with L-Gln, without Ribonucleosides and Deoxyribonucleosides, liquid R 21445-95 500 ml
RPMI 1640 with L-Gln and HEPES, liquid R 30263-95 500 ml
RPMI 1640 with L-Gln, liquid R 30264-85 500 ml
30264-56 10 bottles x 500 ml
RPMI 1640 with L-Gln, without Phenol Red, liquid R 06261-65 500 ml
RPMI 1640 with L-Gln, liquid R 05176-25 500 ml
RPMI 1640 (No Glucose) with L-Gln, liquid R 09892-15 500 ml
(Storage) R = Refrigerator

▲Page top

Balanced Salines

  • Sterilized by 0.2 µm membrane filter, tested for bacteria, fungus, mycoplasma and endotoxin

Ordering information

Product name Storage Product No. PKG Size Online Catalog
D-PBS(+) Preparation Reagent (Ca,Mg Solution) (100x) RT 02492-94 30 ml e-Nacalai
D-PBS(-) without Ca and Mg, liquid  RT  14249-95 500 ml
14249-24  10 bottles
x 500 ml 
D-PBS(-) without Ca and Mg, liquid(10x) R 11482-15 500 ml 
D-PBS(-) without Ca and Mg, Powder RT 07269-84 100 g
HBSS(+) with Ca, Mg and Phenol Red, liquid RT 17459-55 500 ml
HBSS(+) with Ca, Mg, without Phenol Red, liquid RT 09735-75 500 ml
HBSS(-) without Ca and Mg, with Phenol Red, liquid RT 17460-15 500 ml
HBSS(-) without Ca, Mg and Phenol Red, liquid RT 17461-05 500 ml
(Storage) RT = Room Temperature, R = Refrigerator

▲Page top

Supplements

  • Sterilized by 0.2 µm membrane filter, tested for bacteria, fungus, mycoplasma and endotoxin

MEM Non-Essential Amino Acids Solution (100x)

Composition: Non-Essential Amino Acids 10 mM each
L-Alanine 890
L-Asparagine,H2O 1,500
L-Asparatic Acid 1,330
L-Glutamic Acid 1,470
Glycine 750
L-Proline 1,150
L-Serine 1,050
Non-Essential Amino Acids (mg/ml)

▲Page top

100mM-Sodium Pyruvate Solution (100x)

Pyruvic Acid Solution Culture Test

▲Page top

Ordering information

Product name Storage Product No. PKG Size Online Catalog
L-Alanyl-L-glutamine R 01102-82 25 g e-Nacalai
200mmol/l L‐Alanyl‐L‐glutamine Solution(100x) F 04260-64 100 ml
200mM‐L‐Glutamine Stock Solution F 16948-04 100 ml
1mol/l-HEPES Buffer Solution R 17557-94 100 ml
MEM Non-Essential Amino Acids Solution(100x) R 06344-14 20 ml
06344-56 100 ml
100mM-Sodium Pyruvate Solution(100x) R 06977-34 100 ml
apo-Transferrin from Human R 34401-84 100 mg
34401-55 500 mg

(Storage) R = Refrigerator, F = Freezer

Animal Free Cell Media Additives

  • Wide variety of products (recombinant protein, amino Acids, vitamins, others)
Product Type Number of Items Test Items
Recombinant proteins 8 Endotoxin test and other
Amino Acids 28 ・Endotoxin test(max 0.1 EU/mg)
・ Cell culture test
・ Purity
Vitamins 8
Others 4

Ordering information

Product Name Storage Purity Product No. PKG Size Online Catalog
Recombinant proteins 
Albumin, Human, Recombinant expressed in Rice, Animal-Free R 96% 12880-81 1 g e-Nacalai
12880-94 5 g
12880-52 25 g
 [Test item]Endotoxin: max. 1 EU/mg, Solubility in water: Clear to almost clear, Loss on drying: 15%, pH 6.5-7.5 
Insulin, Human, Recombinant expressed in Yeast, Animal-Free F 96% 12878-86 50 mg e-Nacalai
12878-44 250 mg
[Test item] Endotoxin: max. 10 EU/mg, Potency (calculated on dried substance): min. 27.5 units/mg, Loss on drying: max. 10%, Zinc (calculated on dried substance): 0.3-1.0%
Transferrin, Human, Recombinant expressed in Rice, Animal-Free R 95% 12879-34 100 mg e-Nacalai
12879-05 500 mg
12879-21 1 g
[Test item] Endotoxin: max 1.EU/mg, Water: max. 10%, Iron(Fe): max. 0.1 
Amino Acids 
L-α-Alanine, Animal-Free RT 99%(T) 12998-32 25 g e-Nacalai
L-Arginine, Animal-Free RT 98.5%(T) 11984-32 25 g
11984-45 500 g
L-(+)-Arginine Monohydrochloride, Animal-Free RT 99%(T) 12999-22 25 g
L-Asparagine Monohydrate, Animal-Free RT 99%(T) 13000-42 25 g
L-Aspartic Acid, Animal-Free RT 99%(T) 13002-22 25 g
L-Cysteine Hydrochloride Monohydrate, Animal-Free R 99%(T) 11983-42 25 g
11983-55 500 g
L-Cystine Dihydrochloride, Animal-Free RT 98%(T) 13003-12 25 g
L-Glutamic Acid, Animal-Free RT 99%(T) 13012-92 25 g
L-Glutamine, Animal-Free RT 99%(T) 13004-02 25 g
13004-15 500 g
Glycine, Animal-Free RT 99%(T) 12997-42 25 g
L-Histidine (free base), Animal-Free RT 98%(T) 13014-72 25 g
L-Histidine Monohydrochloride Monohydrate, Animal-Free RT 99%(T) 13017-42 25 g
L-Hydroxyproline [trans-4-Hydroxy-L-proline], Animal-Free RT 99%(T) 13018-32 25 g
L-(+)-Isoleucine, Animal-Free RT 99%(T) 13035-02 25 g
L-Leucine, Animal-Free RT 99%(T) 13036-92 25 g
L-Lysine Monohydrochloride, Animal-Free RT 99%(T) 13037-82 25 g
L-Methionine, Animal-Free RT 99%(T) 13038-72 25 g
L-(-)-Phenylalanine, Animal-Free RT 99%(T) 13039-62 25 g
L-(-)-Proline, Animal-Free RT 99%(T) 13040-22 25 g
L-Serine, Animal-Free RT 98%(T) 13041-12 25 g
L-Threonine, Animal-Free RT 99%(T) 13042-02 25 g
L-Tryptophan, Animal-Free RT 99%(T) 13043-92 25 g
L-Tyrosine Disodium Salt Dihydrate, Animal-Free R 98%(T) 11985-22 25 g
11985-35 500 g
L-Valine, Animal-Free RT 98%(T) 13046-62 25 g
Vitamins
p-Aminobenzoic Acid, Animal-Free RT 99%(T) 13047-52 25 g e-Nacalai
L(+)-Ascorbic Acid, Animal-Free RT 99.6%(T) 13048-42 25 g
D-Biotin [Vitamin H], Animal-Free RT 98%(T) 13049-61 1 g
Choline Chloride, Animal-Free R 98%(T) 13050-34 5 g
myo-Inositol [Inosite], Animal-Free RT 99%(GC) 13051-82 25 g
Pyridoxine Hydrochloride, Animal-Free RT 98%(T) 13053-04 5 g
Vitamin B1 Hydrochloride [Thiamine Hydrochloride], Animal-Free RT 98%(HPLC) 13052-14 5 g
Vitamin B12, Animal-Free R 97%(UV) 13054-94 100 mg
Others 
D-(+)-Glucose, Animal-Free RT 98%(GC) 13057-35 500 g e-Nacalai
Glutathione(Reduced Form), free acid, Animal-Free R 97%(T) 13056-61 1 g
Hypoxanthine, Animal-Free RT 97-102%(UV) 13055-71 1 g
Sodium Pyruvate, Animal-Free R 97%(T) 13058-12 25 g

(Storage) RT = Room temperature, R = Refrigerator, F = Freezer

▲Page top

Cell Dissociation Reagents

Ordering information

Product name Storage Product No. PKG Size  Online Catalog
2.5g/l-Trypsin Solution F 35555-54 100 ml e-Nacalai
5.0g/l-Trypsin/5.3mmol/l-EDTA Solution F 35556-44 100 ml
2.5g/l-Trypsin/1mmol/l-EDTA Solution F 35554-64 100 ml
2.5g/l-Trypsin/1mmol/l-EDTA Solution,
with Phenol Red
F 32777-44 100 ml
0.5g/l-Trypsin/0.53mmol/l-EDTA Solution F 35553-74 100 ml
0.5g/l-Trypsin/0.53mmol/l-EDTA Solution,
with Phenol Red
F 32778-34 100 ml
0.2g/l-EDTA Solution R 14367-74 100 ml

(Storage) R = Refrigerator, F = Freezer

▲Page top

Antibiotics

Ordering information

Product name Application Storage Product No. PKG Size Online Catalog
Actinomycin D Solution(1mg/ml) Other Antibiotics F 00393-41 1 ml e-Nacalai
Antibiotic-Antimycotic Mixed
Stock Solution(100x)
Bacteria, Fungal, Yeast F 02892-54 100 ml
Antibiotic-Antimycotic Mixed
Stock Solution(100x)(Stabilized)
Bacteria, Fungal, Yeast F 09366-44 100 ml
Colcemid Solution(10µg/ml) Other Antibiotics R 09356-74 10 ml
G 418 Disulfate Selection Antibiotics RT 16512-36 250 mg
16512-81 1 g
16512-94 5 g
16512-52 25 g
G 418 Disulfate Other Antibiotics RT 08973-01 1 g
08973-14 5 g
G 418 Disulfate Aqueous Solution Selection Antibiotics R 09380-86 20 ml
09380-44 100 ml
Gentamicin Sulfate Bacteria/Mycoplasma R 08975-81 1 g
08975-94 5 g
Gentamicin Sulfate Solution (10mg/ml) Bacteria/Mycoplasma R 16672-04 10 ml
Hygromycin B  Selection Antibiotics  07296-66 100 mg
07296-11 1 g
07296-24   5 g
Hygromycin B Solution Selection Antibiotics R 09287-84 20 ml
Kanamycin Monosulfate Selection Antibiotics RT 08976-71 1 g
08976-84 5 g
Mitomycin C Solution(1mg/ml) Other Antibiotics F 20898-21 1 ml
Penicillin-Streptomycin Mixed Solution
Penicillin 10,000u/ml, Streptomycin 10,000µg/ml
Bacteria(Gram-positive bacteria/
Gram-negative bacteria)
F 26253-84 100 ml
Penicillin-Streptomycin-Glutamine Mixed Solution
Penicillin 10,000u/ml, Streptomycin 10,000µg/ml, L-Glutamine 29.2mg/ml,
Sodium Chloride 0.14%, Citrate Buffer Solution 10mM
Bacteria(Gram-positive bacteria/
Gram-negative bacteria)
F 06168-34 100 ml
Penicillin-Streptomycin Mixed Solution (Stabilized)
Penicillin 10,000u/ml, Streptomycin 10,000µg/ml
Bacteria(Gram-positive bacteria/
Gram-negative bacteria)
F 09367-34 100 ml
Penicillin-Streptomycin Mixed Solution
Penicillin 5,000u/ml, Streptomycin 5,000µg/ml
Bacteria(Gram-positive bacteria/
Gram-negative bacteria)
F 26252-94 100 ml
Streptomycin Sulfate Gram-negative bacteria R 32204-34 5 g
32204-92 25 g

(Storage) RT = Room Temperature, R = Refrigerator, F = Freezer

▲Page top