Bullet PAGE One Precast Gel
Features
- Only 10 minutes with 400 V
- High transfer efficiency of proteins on western blot membrane
- Works well in conventional Laemmli running buffer and sample buffer
- 17-well gel is usable with multichannel pipet for consistent loading
>> Bullet PAGE One Precast Gel vs Laemmli gel 16x (YouTube) 
>> How to remove the comb method 1 (YouTube) , method 2 (YouTube)
Product Information
Performance Comparison
Bullet PAGE One Precast Gel can produce an excellent separation image with the shortest electrophoresis.
| Product Name | Bullet PAGE One | Company A | Company B |
|---|---|---|---|
| Gel ％ | 5 - 15％ | 4 - 15％ | 4 - 12％ |
| Product No. | 13079-84 | - | - |
| Electrophoresis Time | 11 min. | 33 min. | 50 min. |
| Electrophoresis Images |
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Electrophoresis condition
| Sample: | Protein Markers(10x)(Prod No. 29458-24), 5 µl |
| Gel Staining: | CBB Stain One (Prod No. 04543) |
| Voltage Constant: | Bullet PAGE One: 400V, Company A and Company B: 200V |
Comparison of Protein Transfer Efficiency
Bullet PAGE One Precast Gel indicates obviously higher protein transfer efficiency than company A’s, even though blotting time was only 10 min.
Sample
① Protein Ladder One(Triple-color)(Product No. ： 09547-74)
② Protein Markers(3x) 2 µl added
④ Protein Markers(1x) 2 µl added
⑤ Protein Markers(1/3) 2 µl added
Transfer
Use Semi ‐ dry Blotting Buffer Solution for Western Blotting (Product No.: 30650-31)
(Transfer time : 10 min. and 20 min. 10V)
PVDF membrane staining
Stain PVDF membrane with CBB Stain One (Product No. ： 04543).
Gel Types
| Gel | Gradient Gel | Single Percentage Gel | ||
|---|---|---|---|---|
| Gel ％ | 5 - 11% | 5 - 15% | 6% | 8% |
| Product No. 13 well 17 well |
13077-04 13078-94 |
13079-84 13080-44 |
13081-34 13082-24 |
13083-14 13084-04 |
| Electrophoresis Images |  |
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Product Specification
| Glass Plate Size: | W100mm x H80mm x T3.2mm |
| Gel Size: | W80mm x H60mm x T1.0mm |
| Sample Well Configuration / Recommended Load Volume: | 13-well / < 20 µl, 17-well / < 14 µl |
Applications
Coomassie brilliant blue staining and western blotting
The western blot is a composite image using different exposure times for each protein. This data shows that Bullet PAGE One Precast Gel is compatible with CBB staining and western blot techniques.
Data courtesy of professor Tatsuya Moriyama , Faculty of Agriculture, Kinki University
Videos
Bullet PAGE One Precast Gel vs Laemmli gel 16x
How to remove the comb (method 1)
How to remove the comb (method 2)
Protocols
Bullet PAGE One Precast Gel
(100 KB)
Downloads
Bullet PAGE One Precast Gel (PDF 1.5 MB)
Ordering Information
| Product Name | Well | Storage | Product No. | PKG Size | Price |
|---|---|---|---|---|---|
| Bullet PAGE One Precast Gel, 5-11% | 13 | R | 13077-04 | 10 Sheets |  |
| Bullet PAGE One Precast Gel, 5-11% | 17 | R | 13078-94 | 10 Sheets | |
| Bullet PAGE One Precast Gel, 5-15% | 13 | R | 13079-84 | 10 Sheets | |
| Bullet PAGE One Precast Gel, 5-15% | 17 | R | 13080-44 | 10 Sheets | |
| Bullet PAGE One Precast Gel, 6% | 13 | R | 13081-34 | 10 Sheets | |
| Bullet PAGE One Precast Gel, 6% | 17 | R | 13082-24 | 10 Sheets | |
| Bullet PAGE One Precast Gel, 8% | 13 | R | 13083-14 | 10 Sheets | |
| Bullet PAGE One Precast Gel, 8% | 17 | R | 13084-04 | 10 Sheets |
(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze
Related Product
WEP-MN Vertical Electrophoresis Tank
| Product Name: | WEP-MN Vertical Electrophoresis Tank |
| Product No.: | WEP-MN |
| Size: | W134 x D84 x H140 (mm) |
| Required Buffer Volume: | 550 ml |
