新製品情報

ラット抗GFP抗体結合アガロースビーズ

弊社ラット抗GFP抗体は、世界中で使用されており、使用文献も多数報告されています。この度、このラット抗GFP抗体がアガロースビーズに連結された製品を発売しましたので紹介します。

 

特長

  • 好評頂いているラット抗GFP抗体をアガロースビーズに連結

 

使用例

 

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実験概要

  1. EGFPまたはEGFP-myosinを発現するHEK-293細胞のライセート1mlに、抗GFP抗体アガロースを20μlそれぞれ添加し、4℃で2時間免疫沈降した。
  2. SDSサンプルバッファーを加え、90℃で10分間処理し、SDS-PAGEを行った。
  3. 抗GFP抗体を用いてウェスタンブロッティングにより、EGFPとEGFP-myosinを検出した。


データご提供

京都大学　生命科学研究科　高次生命科学専攻　高次生体統御学　垣塚研究室
垣塚　彰　教授、 　堀　清次　講師、 　笹岡　紀男　研究員

 

使用文献

Kumeta, M et al. Molecular mechanisms underlying nucleocytoplasmic shuttling of actinin-4. J Cell Sci. 123(Pt 7):1020-30 (2010).

 

価格表
製品名用途貯法製品番号容量価格買い物
カゴへ
Anti-GFP(Rat IgG2a), Monoclonal(GF090R), CC,Agarose Conjugate IP 冷蔵 06083-05 500μg 55,000 kago.gif
CC = 培養上清、IP = immunoprecipitation

 

参考

ラット抗GFPモノクローナル抗体の使用文献

1 Yonehara, K. Spatially asymmetric reorganization of inhibition establishes a motion-sensitive circuit. Nature. 469(7330):407-10 (2011).
2 Abe, K. et al. Loss of Heterochromatin Protein 1 Gamma Reduces the Number of Primordial Germ Cells via Impaired Cell Cycle Progression in Mice. Biol Reprod 85(5):1013-24(2011).
3 Hide, T. et al. Combination of a ptgs2 inhibitor and an epidermal growth factor receptor-signaling inhibitor prevents tumorigenesis of oligodendrocyte lineage-derived glioma-initiating cells. Stem Cells. 29(4):590-9 (2011).
4 Kurita, S. et al. Cooperative Role of Nectin-Nectin and Nectin-Afadin Interactions in Formation of Nectin-based Cell-Cell Adhesion. J Biol Chem. 286(42):36297-303 (2011).
5 Wang, X. et al. Loss of adenomatous polyposis coli in Bergmann glia disrupts their unique architecture and leads to cell nonautonomous neurodegeneration of cerebellar Purkinje neurons. Glia. 59(6):857-68 (2011).
6 Mochizuki, N. Intravenous injection of neural progenitor cells improves cerebral ischemia-induced learning dysfunction. Biol Pharm Bull. 34(2):260-5 (2011).
7 Inamura, N. et al. Olig2 lineage cells generate GABAergic neurons in the prethalamic nuclei, including the zona incerta, ventral lateral geniculate nucleus and reticular thalamic nucleus. Dev Neurosci. 33(2):118-29 (2011).
8 Vidaki, M. et al. Rac1-Dependent Cell Cycle Exit of MGE Precursors and GABAergic Interneuron Migration to the Cortex. Cereb Cortex. (2011).
9 Konno, A. et al. Evaluation of a Sindbis virus vector displaying an immunoglobulin-binding domain: Antibody-dependent infection of neurons in living mice. Neurosci Res. (2011).
10 Gotoh, H. et al. Genetically-defined lineage tracing of Nkx2.2-expressing cells in chick spinal cord. Dev Biol. 349(2):504-11 (2011).
11 Rubin, AN. et al. The germinal zones of the basal ganglia but not the septum generate GABAergic interneurons for the cortex. J Neurosci. 30(36):12050-62 (2010).
12 Matsumoto, H. et al. Spatial arrangement of glomerular molecular-feature clusters in the odorant-receptor class domains of the mouse olfactory bulb. J Neurophysiol. 103(6):3490-500 (2010).
13 Tamura, H. et al. Atg9A Protein, an Autophagy-related Membrane Protein, Is Localized in the Neurons of Mouse Brains. J Histochem Cytochem. 58(5):443-53 (2010).
14 Asai, R. et al. Endothelin receptor type A expression defines a distinct cardiac subdomain within the heart field and is later implicated in chamber myocardium formation. Development. 137(22):3823-33 (2010).
15 Hirota, Y. et al. Planar polarity of multiciliated ependymal cells involves the anterior migration of basal bodies regulated by non-muscle myosin II. Development. 137(18):3037-46 (2010).
16 Honma, Y. et al. Axonal projections of mechanoreceptive dorsal root ganglion neurons depend on Ret. Development. 137(14):2319-28 (2010).
17 Kohyama, J. BMP-induced REST regulates the establishment and maintenance of astrocytic identity. J Cell Biol. 189(1):159-70 (2010).
18 Manno, A. et al. Enhanced ATPase activities as a primary defect of mutant valosin-containing proteins that cause inclusion body myopathy associated with Paget disease of bone and frontotemporal dementia. Genes Cells. 15(8):911-22 (2010).
19 Mains, RE. et al. Kalrn promoter usage and isoform expression respond to chronic cocaine exposure. BMC Neurosci. 12:20 (2011).

※記載の内容は、'12年1月現在の情報に基づいております。

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