ナカライテスク

製品説明

■Absorption/Emission Spectra of CF™ 488A Conjugated Antibodies

■Technical Summary

極大励起波長 / 極大蛍光波長：490 / 515nm
分子吸光係数：70,000
分子量：〜710
フローサイトメトリーレーザーライン：488nm
顕微鏡レーザーライン：488nm

■CF™ 488A標識二次抗体の蛍光強度の比較

ヒト心臓の凍結組織切片（6µm）を、一次抗体に抗フィブロネクチン抗体、二次抗体に各社蛍光色素標識のGoat Anti-Rabbit IgGを用いて染色しました。その結果、CF™ 488A標識二次抗体は、他社製品と同様に使用できることが示されています。
データ提供：Dr. Sawa Kostin at Max-Planck-Institute für Herz- und Lungenforschung （W.G. Kerckhoff-Institut） in Bad Nauheim（Hessen）-Germany

Jurkat細胞を、一次抗体にintracellular CD3に対する抗体とIsotype、二次抗体にCF™ 488Ａおよび各社蛍光色素標識二次抗体を用いて染色しました。データ中の棒グラフは、Beckman Coulter FC-500で分析した細胞集団の蛍光量の中央値の蛍光強度を測定した結果で、CF™ 488Ａ標識二次抗体は、他社製品と比較し、同等もしくは高い蛍光強度を持つことが示されています。ヒストグラムは、各社蛍光色素：CF™ 488A（緑色）、A社蛍光色素（青色）、Ｃ社蛍光色素（紫色）、D社蛍光色素（赤色）を標識した二次抗体を用いて染色した細胞を、BD FACSCalibur™（FL1 チャネル）によって作成された各ヒストグラムを重ね合わせています。

使用例

■CF™ Dye標識体による多重染色

HeLa細胞を、一次抗体に抗golgin97抗体（マウス）および抗COX IV抗体（ラビット）を用い、それぞれの二次抗体にCF™ 405S標識のGoat Anti-Mouse IgG、CF™ 488A標識のGoat Anti-Rabbit IgGを用いて染色しました。F-アクチンはCF™ 555ファロイジンを用いて染色しました。
青：ゴルジ体、緑：ミトコンドリア、赤：細胞骨格

■CF™ 555 ファロイジン、CF™ 488A標識二次抗体を用いた染色

HeLa細胞を、一次抗体に抗α-チューブリン抗体（マウス）、二次抗体にCF™ 488A標識のGoat Anti-Mouse IgGを用いて染色しました。F-アクチンはCF™ 555ファロイジン、核のDNAはDAPIを用いて染色しました。
緑色：チューブリン、赤色：アクチン、青：核

価格表

製品名	メーカー 製品番号	容量	価格	買い物 カゴへ
CF™ 488A Bovine Anti-Goat IgG (H+L), Highly Cross-adsorbed, 2mg/ml	20293-1	50 µl	13,800
CF™ 488A Bovine Anti-Goat IgG (H+L), Highly Cross-adsorbed, 2mg/ml	20293	0.5 ml	42,800
CF™ 488A Chicken Anti-Goat IgG (H+L), 2mg/ml	20225-1	50 µl	12,000
CF™ 488A Chicken Anti-Goat IgG (H+L), 2mg/ml	20225	0.5 ml	35,800
CF™ 488A Chicken Anti-Mouse IgG (H+L), 2mg/ml	20208-1	50 µl	9,000
CF™ 488A Chicken Anti-Mouse IgG (H+L), 2mg/ml	20208	0.5 ml	31,000
CF™ 488A Chicken Anti-Rabbit IgG (H+L), 2mg/ml	20209-1	50 µl	9,000
CF™ 488A Chicken Anti-Rabbit IgG (H+L), 2mg/ml	20209	0.5 ml	31,000
CF™ 488A Goat Anti-Mouse IgG1, (γ1), 2mg/ml	20246	0.25 ml	46,500
CF™ 488A Goat Anti-Mouse IgG2a, (γ2a), 2mg/ml	20256	0.25 ml	46,500
CF™ 488A Goat Anti-Mouse IgG2b, (γ2b), 2mg/ml	20266	0.25 ml	46,500
CF™ 488A Goat Anti-Mouse IgG (H+L), Highly Cross-adsorbed (min X Rat), 2mg/ml	20302-1	50 µl	18,500
CF™ 488A Goat Anti-Mouse IgG (H+L), Highly Cross-adsorbed (min X Rat), 2mg/ml	20302	0.5 ml	55,800
CF™ 488A Donkey Anti-Chicken IgY (IgG) (H+L), Highly Cross-adsorbed, 2mg/ml	20166-1	50 µl	12,000
CF™ 488A Donkey Anti-Chicken IgY (IgG) (H+L), Highly Cross-adsorbed, 2mg/ml	20166	0.5 ml	38,000
CF™ 488A Donkey Anti-Goat IgG (H+L), Highly Cross-adsorbed, 2mg/ml	20016-1	50 µl	12,000
CF™ 488A Donkey Anti-Goat IgG (H+L), Highly Cross-adsorbed, 2mg/ml	20016	0.5 ml	38,000
CF™ 488A Donkey Anti-Guinea Pig IgG (H+L), Highly Cross-adsorbed, 2mg/ml	20169-1	50 µl	12,000
CF™ 488A Donkey Anti-Guinea Pig IgG (H+L), Highly Cross-adsorbed, 2mg/ml	20169	0.5 ml	38,000
CF™ 488A Donkey Anti-Human IgG (H+L), Highly Cross-adsorbed, 2mg/ml	20074-1	50 µl	12,000
CF™ 488A Donkey Anti-Human IgG (H+L), Highly Cross-adsorbed, 2mg/ml	20074	0.5 ml	38,000
CF™ 488A Donkey Anti-Mouse IgG (H+L), Highly Cross-adsorbed, 2mg/ml	20014-1	50 µl	12,000
CF™ 488A Donkey Anti-Mouse IgG (H+L), Highly Cross-adsorbed, 2mg/ml	20014	0.5 ml	38,000
CF™ 488A Donkey Anti-Rabbit IgG (H+L), Highly Cross-adsorbed, 2mg/ml	20015-1	50 µl	12,000
CF™ 488A Donkey Anti-Rabbit IgG (H+L), Highly Cross-adsorbed, 2mg/ml	20015	0.5 ml	38,000
CF™ 488A Donkey Anti-Rat IgG (H+L), Highly Cross-adsorbed, 2mg/ml	20027-1	50 µl	12,000
CF™ 488A Donkey Anti-Rat IgG (H+L), Highly Cross-adsorbed, 2mg/ml	20027	0.5 ml	38,000
CF™ 488A Donkey Anti-Sheep IgG (H+L), Highly Cross-adsorbed, 2mg/ml	20024-1	50 µl	12,000
CF™ 488A Donkey Anti-Sheep IgG (H+L), Highly Cross-adsorbed, 2mg/ml	20024	0.5 ml	38,000
CF™ 488A Goat Anti-Chicken IgY (IgG) (H+L), Highly Cross-adsorbed, 2mg/ml	20020-1	50 µl	12,000
CF™ 488A Goat Anti-Chicken IgY (IgG) (H+L), Highly Cross-adsorbed, 2mg/ml	20020	0.5 ml	38,000
CF™ 488A Goat Anti-Guinea Pig IgG (H+L), 2mg/ml	20017-1	50 µl	9,000
CF™ 488A Goat Anti-Guinea Pig IgG (H+L), 2mg/ml	20017	0.5 ml	35,800
CF™ 488A Goat Anti-Human IgG (H+L), Highly Cross-adsorbed, 2mg/ml	20022-1	50 µl	12,000
CF™ 488A Goat Anti-Human IgG (H+L), Highly Cross-adsorbed, 2mg/ml	20022	0.5 ml	38,000
CF™ 488A Goat Anti-Mouse IgG (H+L), 2mg/ml	20010-1	50 µl	9,000
CF™ 488A Goat Anti-Mouse IgG (H+L), 2mg/ml	20010	0.5 ml	31,000
CF™ 488A Goat Anti-Mouse IgG (H+L), F(ab')2 Fragment, 2mg/ml	20011	0.25 ml	23,000
CF™ 488A Goat Anti-Mouse IgG (H+L), Highly Cross-adsorbed, 2mg/ml	20018-1	50 µl	12,000
CF™ 488A Goat Anti-Mouse IgG (H+L), Highly Cross-adsorbed, 2mg/ml	20018	0.5 ml	38,000
CF™ 488A Goat Anti-Rabbit IgG (H+L), 2mg/ml	20012-1	50 µl	9,000
CF™ 488A Goat Anti-Rabbit IgG (H+L), 2mg/ml	20012	0.5 ml	31,000
CF™ 488A Goat Anti-Rabbit IgG (H+L), F(ab')2 Fragment, 2mg/ml	20013	0.25 ml	23,000
CF™ 488A Goat Anti-Rabbit IgG (H+L), Highly Cross-adsorbed, 2mg/ml	20019-1	50 µl	12,000
CF™ 488A Goat Anti-Rabbit IgG (H+L), Highly Cross-adsorbed, 2mg/ml	20019	0.5 ml	38,000
CF™ 488A Goat Anti-Rat IgG (H+L), Highly Cross-adsorbed, 2mg/ml	20023-1	50 µl	12,000
CF™ 488A Goat Anti-Rat IgG (H+L), Highly Cross-adsorbed, 2mg/ml	20023	0.5 ml	38,000
CF™ 488A Goat Anti-Swine IgG (H+L), 2mg/ml	20028-1	50 µl	9,000
CF™ 488A Goat Anti-Swine IgG (H+L), 2mg/ml	20028	0.5 ml	31,000
CF™ 488A Rabbit Anti-Chicken IgY (IgG) (H+L), 2mg/ml	20079-1	50 µl	9,000
CF™ 488A Rabbit Anti-Chicken IgY (IgG) (H+L), 2mg/ml	20079	0.5 ml	31,000
CF™ 488A Rabbit Anti-Goat IgG (H+L), 2mg/ml	20021-1	50 µl	9,000
CF™ 488A Rabbit Anti-Goat IgG (H+L), 2mg/ml	20021	0.5 ml	31,000
CF™ 488A Rabbit Anti-Human IgG (H+L), Highly Cross-adsorbed, 2mg/ml	20071-1	50 µl	12,000
CF™ 488A Rabbit Anti-Human IgG (H+L), Highly Cross-adsorbed, 2mg/ml	20071	0.5 ml	38,000
CF™ 488A Rabbit Anti-Mouse IgG (H+L), Highly Cross-adsorbed, 2mg/ml	20026-1	50 µl	12,000
CF™ 488A Rabbit Anti-Mouse IgG (H+L), Highly Cross-adsorbed, 2mg/ml	20026	0.5 ml	38,000
CF™ 488A Rabbit Anti-Rat IgG (H+L), Highly Cross-adsorbed, 2mg/ml	20025-1	50 µl	12,000
CF™ 488A Rabbit Anti-Rat IgG (H+L), Highly Cross-adsorbed, 2mg/ml	20025	0.5 ml	38,000
CF™ 488A Rabbit Anti-Sheep IgG (H+L), Highly Cross-adsorbed, 2mg/ml	20172-1	50 µl	12,000
CF™ 488A Rabbit Anti-Sheep IgG (H+L), Highly Cross-adsorbed, 2mg/ml	20172	0.5 ml	38,000
CF™ 488A Monoclonal Mouse Anti-6X His IgG, 1mg/ml	20228	50 µl	67,700
CF™ 488A Monoclonal Mouse Anti-Biotin, 2mg/ml	20204-1	50 µl	20,200
CF™ 488A Monoclonal Mouse Anti-Biotin, 2mg/ml	20204	0.25 ml	42,800
CF™ 488A Monoclonal Mouse Anti-Fluorescein (FITC) IgG, 2mg/ml	20210-1	50 µl	22,600
CF™ 488A Monoclonal Mouse Anti-Fluorescein (FITC) IgG, 2mg/ml	20210	0.25 ml	46,300
CF™ 488A Monoclonal Mouse Anti-GFP IgG, 1mg/ml	20215	0.1 ml	52,300
Annexin V, CF™ 488A Conjugate, 50µg/ml	29005	0.5 ml	65,000
CF™ 488A Wheat Germ Agglutinin (WGA)	29022-1	1 mg	13,000
CF™ 488A Wheat Germ Agglutinin (WGA)	29022	5×1 mg	54,000
CF™ 488A Concanavalin A (Con A)	29016	5×1 mg	27,000
CF™ 488A Streptavidin	29034	1 mg	38,000
Phalloidin, CF™ 488A Conjugate	00042	300 U	78,000
CF™ 488A α-Bungarotoxin	00005	0.5 mg	72,000
Bovine Serum Albumin, CF™ 488A Conjugate	20289	5×1 mg	37,400
CF™ 488A, Aminooxy	92051	1 mg	82,000
CF™ 488A Amine	92037	1 mg	42,000
CF™ 488A Succinimidyl Ester	92120	1 umole	50,000
CF™ 488A Hydrazide	92152	1 mg	36,000
CF™ 488A Maleimide	92022	1 umole	42,000
Mix-n-Stain™ CF™ 488A antibody labeling kit	92273	1×(5-20µg) labeling	21,000
Mix-n-Stain™ CF™ 488A antibody labeling kit	92253	1×(20-50µg) labeling	24,000
Mix-n-Stain™ CF™ 488A antibody labeling kit	92233	1×(50-100µg) labeling	26,000
CF™ 488A SE protein labeling kit	92213	3 labelings	70,000