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糞便からのDNA精製キット

高収量・高品質のDNA精製が可能

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この製品に関するご意見・ご照会・お問合せはこちら

 

 

 

For the rapid and simple purification of bacterial and host DNA from stool and fecal samples

 

 

 

 

 

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Figure 1. Higher Yields of DNA than Competitor Z.
Stool DNA was isolated from 200 mg of fresh stool samples using Norgens Stool DNA Isolation Kit and Competitor Z's Kit. For evaluation, 10 µL of DNA from the elution was run on 1X TAE 1.2% agarose gel. Norgen's kit isolated much higher yields of DNA. Marker= Norgens HighRanger DNA Ladder.
  
 
 
 
  
  
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Figure 2. Higher Quality DNA than Competitor Z.
Stool DNA was isolated from 200 mg of fresh stool samples using Norgen's Stool DNA Isolation Kit and Competitor Z's Kit. DNA quality was then was measured by Nanodrop. Norgen's Stool DNA Isolation Kit isolated DNA of the highest quality.
  
  
  
  
 
  
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 Figure 3. High Quality DNA confirmed by Real-time PCR.
Stool DNA was isolated from 200 mg of fresh stool samples using Norgen's Stool DNA Isolation Kit and Competitor Z's Kit. DNA quality was confirmed by Real-time PCR using 4 µL of stool DNA (total PCR reaction volume was 20 µL) to detect 16s rDNA from different stool samples. The earlier Ct value with Norgen's DNA samples (blue lines) compared to Competitor Z's samples (red lines) indicated a higher quality of stool DNA for downstream applications.
 
 
 
 
 
 
Kit Specifications
Maximum Stool Input
200 mg
Type of Stool Processed
Frozen, fresh or preserved stool
Format Spin Column
Maximum Column Binding Capacity
50 μg
Maximum Column Loading Volume
650 μL
Elution Volume 50 μL
Time to Complete 10 Purifications
30 minutes
Applications

PCR, qPCR, Southern Blot Analysis,
Sequencing, Microarray Analysis.

 
 
 
Items Cat. # Size
Stool DNA Isolation Kit 27600 50 preps
 
 
 
 
 
For more information visit
https://norgenbiotek.com/product/stool-dna-isolation-kit#
 
この製品に関するご意見・ご照会・お問合せはこちら

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