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Sino Biological社 SARS-CoV-2 Antibodies for Lateral Flow Assay

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SARS-CoV-2_Antibodies_for_Lateral_Flow_Assay_Barner.jpg
While traditional PCR-based assay remains as the golden standard for COVID-diagnosis, there is an increasing demand in other methods that enable quicker, easier, and more cost-effective tests. Lateral flow immunoassay (LFA) is an alternative platform that can be used to detect viral antigens in biological samples. Such tests rely on the availability of antibody pairs that can recognize the target antigen with desired specificity and sensitivity. Hundreds of antibodies against N and S proteins have been made available in recent months. Screening this large resource for a suitable pair has become a major speed limiting factor.
In a recent study supported by The Global Good Fund and Global Health Labs (created by Gates Ventures and the Gates Foundation), authors from Global Health Labs, Intellectual Ventures Laboratory, and PATH systematically evaluated all commercially available antibodies using a high throughput screening process directly on nitrocellulose. The study identified a dozen pairs with the best potential to deliver optimal results in LFA assays.

Index

Capture antibody

Detector antibody

 

Average rank

 

rd.1

rd.2

rd.3

rd.4

Average rank in clinical screen

567

Sino Biological

Sino Biological

1

2.5

25

86.5

-

40143-MM08

40143-R004

527

Sino Biological

Sino Biological

2

30

-

-

-

40143-MM05

40143-MM08

564

Sino Biological

Sino Biological

3

4

21.5

14

12

40143-MM08

40143-MM05

111

Creative Diagnostics

Sino Biological

4.5

46.5

-

-

-

CABT-CS037

40143-R004

423

Genemedi GMP-V-

Sino Biological

5.5

-

-

8.5

2.5

2019nCoV-NAb001

40143-MM08

7

Bioss

Creative Diagnostics

7.5

-

4

5.5

9

bsm-41411M

CABT-CS037

534

Sino Biological

Bioss

7.5

-

12.5

27

-

40143-MM08

bsm-41412M

323

Fitzgerald

Bioss

8

-

43.5

32

44.5

10-2856

bsm-41411M

46

Bioss

Bioss

9.5

-

-

36

23.5

bsm-41413M

bsm-41411M

355

Fitzgerald 10-2856

Sino Biological

9.5

-

3.5

14

14

40143-MM08

640

Sino Biological

Creative Diagnostics

10.5

14

34.5

13.5

-

40143-R040

CABT-CS037

33

Bioss

Sino Biological

11

-

3

5.5

3

bsm-41411M

40143-MM08

401

Genemedi GMP-V-

Bioss

11.5

-

-

23

-

2019nCoV-NAb001

bsm-41413M

517

Novus Bio NB100-

Sino Biological

14.5

87

-

-

-

56683

40143-MM05

666

Sino Biological

Novus Bio

14.5

100

-

-

-

40588-T62

NB100-56683

70

Bioss

Sino Biological

16

-

-

3

3.5

bsm-41413M

40143-MM08

Table: Antibody pairs selected to be screened against clinical samples are ranked according to average performance by S-N and S/N in the clinical screen. Average rank from all four robot screening rounds are also shown.

Ref: David Cate, et al., Antibody Screening Results for Anti-Nucleocapsid Antibodies Towards the Development of a SARS-CoV-2 Nucleocapsid Protein Antigen Detecting Lateral Flow Assay. ChemRxiv.2020

Antibody Pairs for SARS-CoV-2 N Protein

Sino Biological has developed a large panel of antibodies against SARS-CoV-2 N protein. These pairs can be used to assemble detection assays for the corresponding antigen.

Antibody_Pairs_for_SARS-CoV-2_N_Protein.png

Antibody Pairs for N Protein

Antibody Pairs

Capture Ab

Detection Ab

Pair I

40143-R004

40143-R040

Pair II

40143-MM05

40143-R001

Pair III

40143-MM08

40143-R004

Antibodies Specific to SARS-CoV-2 Antigen

One of the main concerns for the antigen tests is whether the antibody pair also detects other circulating coronaviruses. To further validate the specificity, these antibodies were tested against a full panel of N proteins from the common coronaviruses. The result came back as all antibodies against the N proteins have no cross-reactivity in ELISA.

Antibodies_Specific_to_SARS-CoV-2_Antigen.jpg

SARS-CoV-2 N Antibodies

40143-R004
(Rabbit MAb, Rabbit IgG)

40143-R040
(Rabbit MAb, Rabbit IgG)

40143-R001
(Rabbit MAb, Rabbit IgG)

40143-R019
(Rabbit MAb, Rabbit IgG)

40588-R0004
(Rabbit MAb, Rabbit IgG)

40143-MM05
(Mouse MAb, Mouse IgG1)

40143-MM08
(Mouse MAb, Mouse IgG1)

 

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