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Sino Biological社 SARS-CoV-2 Neutralizing MAbs and S2-Specific MAbs

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SARS-CoV-2

Neutralizing Antibodies | S2-Specific Antibodies

Antibody Whitepaper

To support COVID-19 research, Sino Biological has developed a large collection of antibodies against different antigens of SARS-CoV-2. These antibodies can be used to detect and/or neutralize the virus in a variety of assays including ELISA, western blot, immunofluorescent staining, immunohistochemistry, SPR (Biacore and Octet), pseudovirus infection, and potentially in vivo animal studies. 

This week, Sino Biological announces the new additions to this product line:

The publication of SARS-CoV-2 antibody whitepaper.

This guidebook will help the users find the most appropriate antibodies for their assay.

The launch of two mouse mAbs with strong neutralizing activities.

These antibodies are SARS-CoV-2 specific, with no cross-reactivity with SARS-CoV, and other common coronaviruses.

The launch of a chimeric antibody specifically recognizing the S2 domain of SARS-CoV-2.

Full List of SARS-CoV-2 Antibodies

SARS-CoV-2_ANTIBODY_DIRECTORY.jpgGet Free Antibody Guidebook

Specific and Neutralizing Antibodies against SARS-CoV-2 Spike

Cat #  40591-MM43 40592-MM57
Description  Anti-Spike S1 Anti-Spike RBD
Type  Mouse MAb Mouse MAb
Ig Type  Mouse IgG1 Mouse IgG2b
Application  Neutralization, ELISA Neutralization, ELISA

 

Neutralization Activity Assay

Neutralizing antibodies can effectively inhibit the infection of SARS-CoV-2 Spike pseudovirus on ACE2 overexpression 293T cells.

S1 Neutralizing Antibody

 

RBD Neutralizing Antibody

Conc. (µg/mL)

Inhibition%

Conc. (µg/mL)

Inhibition%

100

96.39%

100

95.51%

10

94.57%

10

93.32%

1

42.24%

1

75.69%

0.1

-5.53%

0.1

10.11%

 

Competitive-ELISA Assay

Neutralizing ability was detected by SARS-CoV-2 Inhibitor Screening ELISA Kit (Catalog# KIT001) using competitive ELISA.

S1 Neutralizing Antibody

 

RBD Neutralizing Antibody

S1_Neutralizing_Antibody_Competitive-ELISA_Assay.jpg

RBD_Neutralizing_Antibody_Competitive-ELISA_Assay.jpg

 

Specificity Assay

These two anti-spike antibodies have been tested against all seven coronaviruses, and are shown to be specific to SARS-CoV-2 spike. The reactivity of the antibodies against the known coronaviruses is assessed in ELISA. The reactivity (OD450) is illustrated in the figures below.

S1 Neutralizing Antibody

 

RBD Neutralizing Antibody

S1_Neutralizing_Antibody_Specificity_Assay.jpg

RBD_Neutralizing_Antibody_Specificity_Assay.jpg

 

Antibody Against SARS-CoV-2 Spike S2

Cat #

40590-D001

Description

SARS-CoV-2 Spike S2 Antibody

Type

Chimeric MAb

Ig Type

Mouse V domain & Human IgG1

Cross-reactivity

No cross-reactivity in ELISA with
SARS-CoV-2 (2019-nCoV) Spike S1 (Cat# 40591-V08H)
SARS-CoV-2 (2019-nCoV) Spike RBD (Cat# 40592-V05H)

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