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SinoBiological社 HGF/c-Met Axis: A Promising Therapeutic Target and Biomarker for Cancer

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Mesenchymal-epithelial transition factor (MET), also known as c-Met or Hepatocyte growth factor receptor (HGFR), is a receptor tyrosine kinase (RTK) that is overexpressed or mutated in various malignancies. Aberrantly active MET triggers tumor growth, angiogenesis, and cancer metastasis. MET activity is also critical to embryogenesis.
HGF, also known as scatter factor (SF), is the only known ligand of MET. Upon activation, MET engages with a variety of signal transducers at its intracellular multisubstrate docking site to stimulate the Ras/MAPK, JAK/STAT, SRC, PI3K/AKT, Wnt/ß-catenin, and other signaling pathways. MET and its associated signaling pathways are clinically important therapeutic targets. A number of small-molecule inhibitors and antibodies targeting MET and HGF are being developed as promising therapeutics for cancer, heart disease, autism, and other diseases. Sino Biological has developed high-quality reagents to support the study of MET signaling.
 
c-MET and HGF Proteins
Sino Biological has developed a panel of high activity c-MET proteins and HGF proteins from various species to help study the biology of MET signaling.
Human c-MET Protein | 10692-H20B1   Cynomolgus c-MET Protein | 90304-C08H
WB.png Purity:
> 90% as determined by SDS-PAGE

Activity:
• The specific activity was determined to be 10 nmol/min/mg using MBP as substrate.
• Binding ability with HGF measured by functional ELISA.
Cynomolgus_c-MET.png
Binding ability measured by ELISA. Immobilized human HGF (Cat: 10463-HNAS) at 10 µg/mL (100 µL/well) can bind canine c-MET. The EC50 is 7 ng/mL.
Human HGF Protein | 10463-HNAS Cynomolgus HGF Protein | 90286-CNAH
Human_HGF.png Cynomolgus_HGF.png
Ability to neutralize TGF-beta mediated inhibition on Mv-1-Lu cell proliferation. The ED50 is typically 0.5-5 ng/mL. Ability to inhibit TGFß1 activity on Mv-1-lu mink lung epithelial cells. The ED50 is typically 2-10 ng/mL.
• Recommended c-MET and HGF Proteins
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Catalog #   Species Molecule Tag Expressed Host Activity
10692-H20B1 Human c-MET N-GST & His Insect Cells Active
50622-M02H Mouse c-MET C-human IgG1-Fc HEK293 Cells Active
50622-M08H Mouse c-MET C-His HEK293 Cells Active
70008-D08H Canine c-MET C-His HEK293 Cells Active
80004-R02H Rat c-MET C-human IgG1-Fc HEK293 Cells Active
90304-C02H Cynomolgus, Rhesus c-MET C-human IgG1-Fc HEK293 Cells Active
90304-C08H Cynomolgus, Rhesus c-MET C-His HEK293 Cells Active
90304-CCCH Cynomolgus, Rhesus c-MET C-cleavage HEK293 Cells Active
10463-HNAS Human HGF Native CHO Cells Active
50038-M08H Mouse HGF C-His HEK293 Cells Active
50038-MNAH Mouse HGF Native HEK293 Cells Active
70063-DNAH Canine HGF Native HEK293 Cells  
80429-RNAH Rat HGF Native HEK293 Cells Active
90286-CNAH Cynomolgus HGF Native HEK293 Cells Active
 
Anti-c-MET and Anti-HGF Antibodies
Sino Biological has developed a comprehensive panel of antibodies targeting MET and HGF, which can be used in ELISA, FCM, IHC, WB, as well as neutralization. ELISA kits and ELISA pair sets are also available.
Cell Proliferation was Inhibited by Human HGF Antibody | 10463-mh010 Flow Cytometric Analysis of Human c-Met Expression on HepG2 Cells | 10692-R243
Cell_Proliferation_was_Inhibited.png Flow_Cytometric_Analysis.png
Proliferation of U87 MG cells elicited by autocrine HGF was inhibited by increasing concentrations of Human HGF Neutralizing Monoclonal Antibody. The IC50 is typically 1-4 µg/mL. Cells were stained with purified anti-Human c-Met, second antibody conjugated with FITC.
• More Antibodies of c-MET and HGF
Anti-c-MET Antibodies   Anti-HGF Antibodies

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