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SinoBiological社 Recombinant Growth Factors for Organoid Culture

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Organoids are 3D multicellular in vitro tissue cultures that mimic their corresponding in vivo organs. Such cultures can be crafted to resemble much of the complexity of an organ, therefore, are changing the way diseases are studied and treated. Organoid technology has recently emerged as an essential tool for both fundamental and biomedical research.
Organoids can be derived from embryonic stem cells (ESCs), induced pluripotent stem cells (iPSCs), or primary tissue. Different media components are required for each type of source cells used and the type of differentiation to be achieved. Growth factors, such as EGF, Noggin (NOG), R-spondin (RSPO1), HGF, BMP, and FGF, are essential components of organoid media.
Type of Tissue Source Stem Cell Culture Condition (niche factors) Differentiation Culture Condition
Stomach Adult mouse EGF, Noggin, R-spondin, Wnt-3A, FGF10 EGF, R-spondin EGF, R-spondin
Adult human EGF, Noggin, R-spondin, Wnt-3A, FGF10  
hPSC EGF EGF
 
Small Intestine Adult mouse EGF, Noggin, R-spondin EGF, Noggin, R-spondin
Adult human EGF, Noggin, R-spondin, Wnt-3A TGF-beta inhibitor, p38 inhibitor EGF, Noggin, R-spondin, TGF-beta inhibitor
hPSC EGF EGF
 
Colon Adult mouse EGF, Noggin, R-spondin, Wnt-3A EGF, Noggin, R-spondin
Adult human EGF, Noggin, R-Spondin, Wnt-3A, TGF-beta inhibitor, p38 inhibitor EGF, Noggin, R-spondin, TGF-beta inhibitor
 
Pancreas Adult mouse EGF, Noggin, R-spondin, Wnt-3A, FGF10, Nicotinamide EGF, Noggin, R-spondin, Wnt-3A
Adult human EGF, Noggin, R-spondin, Wnt-3A, FGF10, TGF-beta inhibition, Nicotinamide Not reported
 
Liver Adult mouse EGF, Noggin, R-spondin, Wnt-3A, FGF10, HGF, Nicotinamide EGF, Noggin, FGF10, TGF-beta inhibition, Notch inhibition
Adult human EGF, Noggin, R-spondin, Wnt-3A, FGF10, HGF, Nicotinamide, TGF-b inhibitor, Forskolin EGF, Noggin, FGF10, TGF-beta inhibition, Notch inhibition, BMP7
Table 1: Culture Conditions for Organoids from Stem Cells (Source: Cell)
Sino Biological has developed a panel of recombinant growth factors with high bioactivity, batch-to-batch consistency, and purity to enable optimal and consistent organoid growth.
 
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Recombinant Growth Factor Proteins for Organoid Culture
Sino Biological has developed bioactive recombinant growth factors from various species, such as human, mouse, cynomolgus, rhesus, rat, and canine.
• Featured EGF Protein
Human EGF Protein: 10605-HNAE
 
High-purity   Low-endotoxin
 
Tag-free   HPLC-verified
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> 95 % as determined by SEC-HPLC
 
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A cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblasts Batch-to-batch Consistency
• Other Featured Growth Factors
Recombinat HGF Proteins
Human HGF Protein | 10463-HNAS Cynomolgus HGF Protein | 90286-CNAH
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Ability to neutralize TGF-beta mediated inhibition on Mv-1-Lu cell proliferation. Ability to inhibit TGFß1 activity on Mv-1-lu mink lung epithelial cells.
 
Human FGF10 Protein | 10573-HNAE Human Noggin/NOG Protein | 10267-HNAH
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Cell proliferation assay using BaF3 mouse proB cells transfected with human FGFR2b. Ability to inhibit BMP4-induced alkaline phosphatase production by MC3T3E1 mouse preosteoblast cells.
 
Human RSPO1 Protein | 11083-HNAS Human BMP-2 Protein | 10426-HNAE
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Ability to induce activation of ßcatenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. Ability to induce alkaline phosphatase production by MC3T3-E1 mouse osteoblastic cells.
 
More Recombinant Growth Factors for Organoid Culture
EGF   FGF2   FGF7   FGF9   FGF10
 
HGF   NOG   RSPO1   RSPO3   Activin A
 
BMP2   BMP4    
Sino Biological

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