Products

COSMOSIL Sugar-D

Features

Column Image

  • Different selectivity from aminopropyl columns
  • Superior quantitative results
  • Superior durability
  • Anomers remain unseparated

Product Information

Column Specifications

Column Specifications

Features

Sugar-D_Feat.png

About Sugar-D

Development of Sugar-D

Aminopropyl columns are commonly used for analysis of monosaccharides and oligosaccharides; however, this type of column has several problems, including low durability, poor separation, and adsorption and peak tailing with some analytes. Carbamoylbased columns separate anomers, which may not be desirable. To solve these problems, we developed a specialty column for sugar analysis with high durability and performance that does not induce irreversible adsorption.

Sugar-D_name.png

Different selectivity from aminopropyl columns

COSMOSIL Sugar-D has a polyamine-based stationary phase, which results in different selectivity from primary anime-based phases, such as aminopropyl.

Comparison to aminopropyl columns

• Sucrose and Turanose

Sugar-D_18App1.png

Condition
Column Size 4.6 mm I.D. x 250 mm
Flow Rate 1 ml/min
Temperature 30°C
Detection RI
Sample
1; Fructose (26.5mg/ml)
2; Glucose (21.2mg/ml)
3; Sucrose (5.3mg/ml)
4; Turanose (5.9mg/ml)
5: Maltose (5.3mg/ml)
Inj.Vol. 17‹µl

Superior quantitative results

Aldehyde groups in the open-chain form of sugars can form Schiff bases with the amino groups on aminopropyl columns, causing low recovery and peak tailing. COSMOSIL Sugar-D was designed to avoid this, so sugars like arabinose and galactose, which are problematic on aminopropyl columns, elute with sharp peaks. Sugar-D is especially useful for samples of low concentration.

Quantitation at low concentration

Sugar-D_Quantitation.png

Superior durability

The stationary phase of COSMOSIL Sugar-D is not easily hydrolyzed, so even using water as the mobile phase does not affect it much.

Change in retention factor

Sugar-D_18retention-factor.png

Eluent Condition
Eluent Water
Flow Rate 1 ml/min
Temperature RT
Test Condition
Column Size 4.6 mm I.D. x 250 mm
Mobile Phase Acetonitrile : Water = 70 : 30
Flow Rate 1.0 ml/min
Temperature 30°C
Detection RI
Sample Maltose

Anomers remain unseparated

When analyzing sugars with amide columns, anomers may separate unless harsh conditions, such as high temperature and basic solvents, are employed. COSMOSIL Sugar-D does not separate anomers, even under mild conditions.

Comparison to amide column

Sugar-D_Comparison_Amino.png

Test Condition
Column Size 4.6 mm I.D. x 250 mm
Mobile Phase (Sugar-D) Acetonitrile / H2O = 80/20
(Company E) Acetonitrile / H2O = 75/25
Flow Rate 1.0 ml/min
Temperature 30°C
Detection RI
Sample
1; Rhamnose (10‹µg)
2; Xylose (10‹µg)
3; Fructose (10‹µg)
4; Mannose (10‹µg)
5; Glucose (10‹µg)

Applications

Analysis of Carbohydrate Mixtures by COSMOSIL Sugar-D

We have compiled a list of our Sugar-D HPLC application data in an easy-to-read format. Please click the compound name to view its data.

CoumpoundCAS No.No. of applications
D-(+)-Glucose 50-99-7 11
D-(-)-Fructose 57-48-7 9
Xylitol 87-99-0 4
D-Psicose 551-68-8 3
Sucrose 57-50-1 7
Maltose 6363-53-7 5
D-(+)-Raffinose 17629-30-0 2
D-(+)-Mannose 3458-28-4 2
L-(+)-Rhamnose 10030-85-0 2
D-(+)-Trehalose 6138-23-4 1
D-(+)-Allose 2595-97-3 1
D-Glucitol 50-70-4 3
Maltitol 585-88-6 3
meso-Erythritol [meso-Erythrite] 149-32-6 3
myo-Inositol 87-89-8 2
Cyclodextrins 10016-20-3
7585-39-9
17465-86-0
1
D-Glucuronic Acid 6556-12-3 1
Glycerol 56-81-5 1
1-Kestose 470-69-9 1
Lactose 5989-81-1 1
Maltotriitol 32860-62-1 2
Maltotriose 1109-28-0 2
Maltotetraose 34612-38-9 1
Maltopentaose 1668-09-3 1
Maltohexaose 34620-77-4 1
Maltoheptaose 34620-78-5 1
D-(-)-Mannitol 69-65-8 1
D-Mannosamine 5505-63-5 1
Nystose 13133-07-8 1
Palatinit 64519-82-0 1
Palatinose 13718-94-0 1
D-(+)-Xylose 58-86-6 1
1-Fructofuranosyl-D-nystose 1
Fructooligosaccharides 1
Isomaltooligosaccharides 1
Maltotertaitol 1
Xylooligosaccharides 1

Sugars in Sports Drinks

Sugar-D_18App_Drinks.png
Condition
Column Size (4.6 mm I.D. x 250 mm) x2
Mobile Phase Acetonitrile/ H2O = 75/25
Flow Rate 1.0 ml/min
Temperature 50°C
Detection RI
Sample
1; meso-Erythritol
(meso-Erythrite)
(10‰µg)
2; D-(-)-Fructose (10‰µ‰g)
3; D-Glucitol (10‰µ‰g)
4; D-(+)-Glucose (10‰‰µg)
5; Sucrose (10‰µ‰g)
6; Maltose (10‰µ‰g)
7; Maltotriitol (10‰‰µg)
8; Maltotriose (10‰‰µg)

Honey

Sugar-D_18App_Honey.png

Condition
Column Size 4.6 mm I.D. x 250 mm
Mobile Phase Acetonitrile/ H2O = 75/25
Flow Rate 1.0 ml/min
Temperature 30°C
Detection RI
Sample Honey (50mg/ml)
1; D-(-)-Fructose
2; D-(+)-Glucose
Inj.Vol.: 5.0µl

Sugar Alcohols

Sugar-D_18App_Alcohols.png

Condition
Column Size 4.6 mm I.D. x 250 mm
Mobile Phase Acetonitrile/ H2O = 75/25
Flow Rate 1.0 ml/min
Temperature 30°C
Detection RI
Sample
1; Glycerol (10µg)
2; meso-Erythritol
(meso-Erythrite)
(10µg)
3; Xylitol (10µg)
4; D-Glucitol (10µg)
5; Maltitol (10µg)
6; myo-Inositol (10µg)

Maltooligosaccharides

Sugar-D_18App_Maltoo.png

Condition
Column Size 4.6 mm I.D. x 250 mm
Mobile Phase Acetonitrile/ H2O = 65/35
Flow Rate 1.0 ml/min
Temperature 30°C
Detection RI
Sample
1; D-(+)-Glucose (10‰µg)
2; Maltose (10‰µg)
3; Maltotriose (10‰µg)
4; Maltotetraose (10‰µg)
5; Maltopentaose (10‰µg)
6; Maltohexaose (10‰µg)
7; Maltoheptaose (10‰µg)

See more COSMOSIL Applications

Usage information

Column 5 µm Sugar-D
I.D. (mm) 2.0 3.0 4.6 10.0 20.0
Washing method
  1. Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. For example, if your mobile phase was 50:50 acetonitrile/20 mmol/l phosphate buffer, wash with 50:50 acetonitrile/water.
  2. Remove adsorbed compounds and fix unstable baselines: Wash with 50:50 acetonitrile/water or up to 100% water.
Storage conditions Wash the column to remove buffer, salts and/or acid.
Then, replace the solvent with 90:10 acetonitrile/water.

Tightly plug the column, and store in a cool and dry place.
Usable pH range pH 2 - 7.5
Max. pressure 20MPa 15MPa
Temperature range The maximum usable temperature is 60°C. However. for regular use, please use at a constant temperature between 20°C and 50°C.
Usable solvents Acetonitrile/water mobile phases are recommended.
Other instructions
  • Buffer concentration is usually sufficient at 0.005 - 0.02 mol/L.
  • Always filter mobile phases using a 0.5 µm filter before use.
  • Methanol is not recommended as a mobile phase.
  • If using a buffer or acid, we recommend using the column only for that mobile phase. Buffers may affect performance even after washing the column.
  • Neutral pH will maximize retention time.

Inseparable Compounds

Some sugars are not separated well by Sugar-D. For these compounds, you can use COSMOSIL NH2-MS.

Compound pair NH2 application
Glucose Galactose AP-0137
Lactose Maltose AP-0138

You can search for others in our application search. The Sugar-D chromatogram index (applications beginning "K-") can help you determine analysis conditions.

Downloads

Brochures

References

Reference list

Ordering Information

Analytical and Preparative Column (Particle size : 5 µm)

COSMOSIL Sugar-D

Product Name Size Product No. Price
COSMOSIL Sugar-D Guard Column
4.6 mm l.D. x 10 mm 05394-81 e-Nacalai
10 mm l.D. x 20 mm 05696-31
20 mm l.D. x 50 mm 05694-51
COSMOSIL Sugar-D Packed Column
2.0 mm l.D. x 250 mm 05689-31 e-Nacalai
3.0 mm l.D. x 150 mm 05690-91
3.0 mm l.D. x 250 mm 05691-81
4.6 mm l.D. x 150 mm 05395-71
4.6 mm l.D. x 250 mm 05397-51
10 mm l.D. x 250 mm 05692-71
20 mm l.D. x 250 mm 05693-61

(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze