Products

Ampdirect Plus

Ampdirect Plus is a novel PCR buffer that effectively neutralizes DNA polymerase inhibitors present in tissues or body fluids from plants or animals, and allows direct PCR amplification from as little as 0.5 µl of sample.

* Ampdirect Plus is produced by SHIMADZU BIOTECH in JAPAN.

Features

  • Direct PCR amplification from tissues or body fluids (including blood) from plants or animals
  • No DNA extraction required
  • Minimizes the risk of sample cross contamination
  • Requires small volume of blood (0.5 - 1 µl)
  • Compatible with various anticoagulants (including sodium citrate, dipotassium EDTA and sodium heparinate)
  • Compatible with blood stored frozen for long periods
  • Ideal for genotyping and genetic screening experiments

Principle

principle

Application

Application 1

Direct PCR of 5 Genes from 23 Different Human Blood Samples

  • PCR: 23 different human blood samples (1L sample volumes treated with EDTA was added to 50 l of Ampdirect Plus mixture)
  • Lane M: Molecular size marker

Application 1

Application 2

Direct PCR from fresh and frozen blood

  • Target: Human beta-globin gene
  • PCR: 5 l (lane 1), 2.5 l (lane 2), 1.25 l (lane 3), 0.63 l (lane 4) and 0 l (lane N) of human blood / 50 l of Ampdirect mixture
  • Molecular size marker: ΦX174 RF DNA digested with HincII (lane M)

Application 2

Protocols

Ampdirect Plus Procedure (PDF)

Downloads

SDS: Ampdirect Plus (PDF)

References

  1. Rapid and simple mutation screening of GM1 gangliosidosis in Shiba dogs by direct amplification of deoxyribonucleic acid from various forms of canine whole-blood specimens
    Yamato O, et al.: J Vet Diagn Invest 2004;16: 469-72

  2. Prenatal origin of TEL-AMLI-positive acute lymphoblastic leukemia in children born in California
    McHale CM, et al GENES, Chromosomes & Cancer 2003;37: 36-43

  3. In utero origin of t(8;21) AML1-ETO translocations in childhood acute myeloid leukemia
    Wiemels JL, et al.: Blood 2002;99: 3801-5

  4. Site-specific translocation and evidence of postnatal origin of the t(1;19) E2A-PBX1 fusion in childhood acute lymphoblastic leukemia
    Wiemels JL, et al.: Proc. Natl. Acad. Sci. USA 2002;99: 15101-6

  5. Various applications of direct PCR using blood samples
    Nishimura N, et al.: Clin Lab; 2002;48: 377-84

  6. Efficiency of polymerase chain reaction using a novel method of DNA preparation and amplification for detection of Mycobacterium avium subsp. paratuberculosis in bovine fecal samples
    Kojima K, et al.: Proceedings of the Seventh International Colloquium on Paratuberculosis; 2002; 267-9

  7. Major change in the predominant type of "Norwalk-like viruses" in outbreaks of acute nonbacterial gastroenteritis in Osaka city, Japan, between April 1996 and March 1999
    Iritani, et al.: J Clin Microbiol 2000;38: 2649-54

  8. Detection of clonotypic IGH and TCR rearrangements in the neonatal blood spots of infants and children with B-cell precursor acute lymphoblastic leukemia
    Yagi T, et al.: Blood; 2000;96:264-8

  9. Direct PCR from whole blood without DNA isolation
    Nishimura N, et al.: Ann Clin Biochem; 2000;37: 674-80

  10. Association between platelet glycoprotein Iba genotype and ischemic cerebrovascular disease
    Sonoda A, et al.: Stroke 2000;31: 493-7

  11. Prenatal origin of acute lymphoblastic leukaemia in children
    Wiemels JL, et al.: Lancet 1999;354: 1499-503

  12. Direct amplification of Escherichia coli O157 vero toxin genes from human faeces by the polymerase chain reaction
    Okamoto H, et al.: Ann Clin Biochem 1999;36: 642-8

Ordering Information

Product Name Grade Storage Product No. PKG Size
Ampdirect Plus F 241-08800-98 500 tests
Ampdirect Plus / BIOTAQ HS DNA Polymerase Set F 241-08890-92 500 tests

(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze