RNase Quiet / RNase Quiet for Replacement
RNase Quiet is a ready-to-use solution for eliminating RNase, DNase, DNA and RNA contamination. It completely removes RNase contamination from glass, plastic equipments and laboratory tables.
Features
- Removes RNase contamination effectively
- Easy to use spray type
- Easy to wipe with no detergent
- Non-carcinogenic with no DEPC
Applications
Decontamination of cover glass
Condition
- Apply 100 µl RNase A solution (1 mg/ml) on cover glasses and dry them.
- Spray with DEPC treated water or RNase Quiet and wait for 1 minute. Wipe thoroughly with a clean paper towel and then rinse with RNasefree sterile water.
- Apply 50 µl RNA Solution (40 µg/ml) on the cover glasses and incubate them at 37°C for 30 minutes.
- Apply 1 µl ethidium bromide solution (20 µg/ml) on the cover glasses with a pipette.
- Observe the cover glasses with UV.
Decontamination of 1.5 ml micro-tubes
Condition
- (+): Add 10 µl RNase A solution (10 mg/ml) in 1.5 ml micro-tubes.
(?): Add 10 µl lysate buffer in 1.5 ml micro-tubes. - Add 1 ml DEPC treated water or RNase Quiet and wait for 1 minute. Remove the solution from the tube.
- Add 25 µl RNA Solution (40 µg/ml) and incubate them at 37°C for 30 minutes.
- Analyze RNA solution by electrophresis with 1% agarose gel including 100 ng/ml ethidium bromide and then staining gel with 200 ng/ml ethidium bromide solution.
Downloads
Ordering information
| Product Name | Storage | Product No. | PKG Size | Price |
|---|---|---|---|---|
| RNase Quiet (with spray nozzle) |
Room Temp. |
09147-14 | 475 ml |  |
| RNase Quiet for Replacement | Room Temp. |
09477-94 | 475 ml |
Related Products
| Product Name | Storage | Product No. | PKG Size | PRICE |
|---|---|---|---|---|
| 100g/l-Hexadecyltrimetylammonium Bromide Solution, Nuclease tested | Room Temp. |
17472-94 | 100 ml | |
| Phenol:Chloroform:Isoamyl Alcohol 25:24:1 Mixed, pH5.2 | 4°C | 26058-54 26058-96 |
100 ml 400 ml |
|
| Water, DEPC treated, RNase tested | Room Temp. |
36420-61 36420-74 36415-54 36415-41 |
10 x 1 ml 50 x 1 ml 100 ml 1 L |
|
| Hexadecyltrimethylammonium Bromide | Cool and Dark |
08897-82 | 25 g | |
| Guanidine Thiocyanate | Room Temp. |
06287-32 06287-45 |
25 g 500 g |
|
| Ethanol(99.5) | Room Temp. |
08948-54 08948-25 |
100 ml 500 ml |
|
(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze
For research use only, not intended for diagnostic or drug use.
