Ubiquitin/Ubl Conjugation Assays (LifeSensors)
The conjugation of ubiquitin to substrates is a multi-step, multi-enzyme process. LifeSensors is committed to developing high throughput, facile, and robust assays for measuring the activity of ubiquitin conjugating enzymes. LifeSensors has recently developed a microtiter plate-based assay for measuring protein ubiquitylation. This assay is amenable to high throughput screening and works with a wide range of E3 ligases including members of the three major classes of E3 ligases (simple RING class, SCF RING class, and HECT class)
Product Information
E3LITE Customizable Ubiquitin Ligase Kit / E2 Profiling Kit
They are essentially providing a singular platform for the focused investigation of any E2/E3 enzyme pair, as well as any particular polyubiquitylation linkage type. In support of this flexible platform, LifeSensors offers the widest array of reagents on the market to meet your research needs. The kit itself can be assembled with any one of more than E2 conjugation enzymes, with a selection representing members from each enzyme class. LifeSensors also offers E2 Selection Kits that provides a panel of (4) E2s. The recombinant ubiquitin component of this kit can be wild-type, variants that specifically form K48 linkage types, or variants that form linkages only through one of the seven possible surface exposed lysines.
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E3 Substrate Identification Array Support Kit
This kit provides reagents and protocols for array-based discovery of E3 substrates, including all components necessary for substrate ubiquitylation as well as LifeSensors' proprietary detection reagent for polyubiquitin chains. The user must provide a protein microarray and FITC conjugated avidin/streptavidin. LifeSensors has developed an extremely sensitive poly-ubiquitin detection reagent with nanomolar affinity for poly-ubiquitin chains. Utilizing this detection reagent in combination with protein microarrays allows the user to identify potential E3 ligase substrates.
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Ordering Information
| Product Name | Product No. |
|---|---|
| E3LITE Customizable Ubiquitin Ligase Kit | UC101 |
| E2 Profiling Kit | UC102 |
| E3 Substrate Identification Array Support Kit | UC103 |
(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze
