製品情報

TriLink 社 修飾ヌクレオチド

修飾ヌクレオチドは、核酸医薬研究、エピジェネティクス研究、再生医療研究などさまざまな研究分野で応用されています。TriLink 社は、目的に応じた多種多様な修飾ヌクレオチドを販売しています。ここでは以下の研究分野で利用されている製品を紹介します。

核酸医薬研究(アプタマー、アンタゴ mir、siRNA)

修飾されたヌクレオチドをアプタマー、アンタゴ mir、siRNA の合成に利用することで、ターゲットへの特異性やヌクレアーゼに対する耐性を向上させる一方で、免疫応答を低減することができます。TriLink 社では核酸医薬研究に有用な修飾ヌクレオチドを取り扱っています。

2'-Fluoro-2'-deoxycytidine-5'-triphosphate 8-Oxo-2'-deoxyguanosine-5'-triphosphate
N-1008.png N-2034.png

使用文献(一部)

  1. Dupont, D. M. et al. Protein-binding RNA aptamers affect molecular interactions distantly from their binding sites. PLOS ONE 10(4), e0126782 (2015).
  2. Kariolis, M. S. et al. An engineered Axl 'decoy receptor' effectively silences the Gas6-Axl signaling axis. Nat Chem Biol. 10(11), 977-83 (2014).
  3. Mizrahi, R. A. et al. Potent and selective inhibition of A-to-I RNA editing with 2'-O-methyl/locked nucleic acid-containing antisense oligoribonucleotides. ACS Chem Biol. 8(4), 832-9 (2013).

価格表

製品名メーカー
製品番号
容量オンライン
カタログへ
2'-Fluoro-2'-deoxycytidine-5'-triphosphate*1 N-1008-1 1 µmole e-Nacalai.jpg
N-1008-5 5 µmoles e-Nacalai.jpg
N-1008-10 10 µmoles e-Nacalai.jpg
N-1008-50 5 X 10 µmoles e-Nacalai.jpg
N-1008-100 10 X 10 µmoles e-Nacalai.jpg
2'-Fluoro-2'-deoxyuridine-5'-triphosphate N-1010-1 1 µmole e-Nacalai.jpg
N-1010-5 5 µmoles e-Nacalai.jpg
N-1010-10 10 µmoles e-Nacalai.jpg
N-1010-50 5 X 10 µmoles e-Nacalai.jpg
N-1010-100 10 X 10 µmoles e-Nacalai.jpg
2'-O-Methylguanosine-5'-triphosphate N-1017-1 1 µmole e-Nacalai.jpg
N-1017-5 5 µmoles e-Nacalai.jpg
N-1017-10 10 µmoles e-Nacalai.jpg
Inosine-5'-triphosphate N-1020-1 1 µmole e-Nacalai.jpg
N-1020-5 5 µmoles e-Nacalai.jpg
N-1020-10 10 µmoles e-Nacalai.jpg
2'-O-Methylinosine-5'-triphosphate N-1021-1 1 µmole e-Nacalai.jpg
N-1021-5 5 µmoles e-Nacalai.jpg
N-1021-10 10 µmoles e-Nacalai.jpg
2'-Azido-2'-deoxycytidine-5'-triphosphate N-1028-1 1 µmole e-Nacalai.jpg
N-1028-5 5 µmoles e-Nacalai.jpg
N-1028-10 10 µmoles e-Nacalai.jpg
Arauridine-5'-triphosphate N-1034-1 1 µmole e-Nacalai.jpg
N-1034-5 5 µmoles e-Nacalai.jpg
N-1034-10 10 µmoles e-Nacalai.jpg
5-Bromo-2'-deoxyuridine-5'-triphosphate N-2008-1 1 µmole e-Nacalai.jpg
N-2008-5 5 µmoles e-Nacalai.jpg
N-2008-10 10 µmoles e-Nacalai.jpg
5-Nitro-1-indolyl-2'-deoxyribose-5'-triphosphate N-2031-1 1 µmole e-Nacalai.jpg
N-2031-5 5 µmoles e-Nacalai.jpg
N-2031-10 10 µmoles e-Nacalai.jpg
8-Oxo-2'-deoxyguanosine-5'-triphosphate*2 N-2034-1 1 µmole e-Nacalai.jpg
N-2034-5 5 µmoles e-Nacalai.jpg
N-2034-10 10 µmoles e-Nacalai.jpg
5-[(3-Indolyl)propionamide-N-allyl]-2'-deoxyuridine-5'-triphosphate N-2065-0250 250 nmoles e-Nacalai.jpg
N-2065-1 1 µmole e-Nacalai.jpg
N-2065-5 5 µmoles e-Nacalai.jpg
3'-Deoxyadenosine-5'-triphosphate N-3001-1 1 µmole e-Nacalai.jpg
N-3001-5 5 µmoles e-Nacalai.jpg
N-3001-10 10 µmoles e-Nacalai.jpg
3'-Deoxyguanosine-5'-triphosphate N-3002-1 1 µmole e-Nacalai.jpg
N-3002-5 5 µmoles e-Nacalai.jpg
N-3002-10 10 µmoles e-Nacalai.jpg
3'-Deoxycytidine-5'-triphosphate N-3003-1 1 µmole e-Nacalai.jpg
N-3003-5 5 µmoles e-Nacalai.jpg
N-3003-10 10 µmoles e-Nacalai.jpg
3'-Deoxyuridine-5'-triphosphate N-3005-1 1 µmole e-Nacalai.jpg
N-3005-5 5 µmoles e-Nacalai.jpg
N-3005-10 10 µmoles e-Nacalai.jpg
2',3'-Dideoxyguanosine-5'-triphosphate*3 N-4002-1 1 µmole e-Nacalai.jpg
N-4002-5 5 µmoles e-Nacalai.jpg
N-4002-10 10 µmoles e-Nacalai.jpg
3'-Azido-2',3'-dideoxyadenosine-5'-triphosphate N-4007-1 1 µmole e-Nacalai.jpg
N-4007-5 5 µmoles e-Nacalai.jpg
N-4007-10 10 µmoles e-Nacalai.jpg
3'-Azido-2',3'-dideoxyguanosine-5'-triphosphate N-4008-1 1 µmole e-Nacalai.jpg
N-4008-5 5 µmoles e-Nacalai.jpg
N-4008-10 10 µmoles e-Nacalai.jpg
3'-Azido-2',3'-dideoxythymidine-5'-triphosphate N-4009-1 1 µmole e-Nacalai.jpg
N-4009-5 5 µmoles e-Nacalai.jpg
N-4009-10 10 µmoles e-Nacalai.jpg
Guanosine-3',5'-bisdiphosphate N-6001-1 1 µmole e-Nacalai.jpg
N-6001-5 5 µmoles e-Nacalai.jpg
N-6001-10 10 µmoles e-Nacalai.jpg
N-6001-50 5 X 10 µmoles e-Nacalai.jpg
N-6001-100 10 X 10 µmoles e-Nacalai.jpg
2'-Deoxyadenosine-5'-O-(1-thiotriphosphate) N-8001-1 1 µmole e-Nacalai.jpg
N-8001-5 5 µmoles e-Nacalai.jpg
N-8001-10 10 µmoles e-Nacalai.jpg
2'-Deoxycytidine-5'-O-(1-thiotriphosphate) N-8002-1 1 µmole e-Nacalai.jpg
N-8002-5 5 µmoles e-Nacalai.jpg
N-8002-10 10 µmoles e-Nacalai.jpg
2'-Deoxyguanosine-5'-O-(1-thiotriphosphate) N-8003-1 1 µmole e-Nacalai.jpg
N-8003-5 5 µmoles e-Nacalai.jpg
N-8003-10 10 µmoles e-Nacalai.jpg
2'-Deoxythymidine-5'-O-(1-thiotriphosphate) N-8004-1 1 µmole e-Nacalai.jpg
N-8004-5 5 µmoles e-Nacalai.jpg
N-8004-10 10 µmoles e-Nacalai.jpg

核酸医薬研究(抗ウイルス研究)

抗ウイルス剤は HIV や腫瘍、がんなどに関与するレトロウイルスの逆転写酵素を阻害することで作用します。修飾ヌクレオチドはウイルス DNA に取り込まれ、二本鎖のウイルス DNA の合成を抑制します。TriLink 社では抗ウイルス研究に有用な修飾ヌクレオチドを取り扱っています。

2',3'-Dideoxycytidine-5'-triphosphate2'-Deoxyadenosine-5'-O-(1-boranotriphosphate)
N-4005.png N-8050.png

使用文献(一部)

  1. Smith, T. M. et al. Identifying initiation and elongation inhibitors of dengue virus RNA polymerase in a high-throughput lead-finding campaign. J Biomol Screen. 20(1), 153-63 (2015).
  2. Cheek, M. A. et al. Inhibition of hepatitis C viral RNA-dependent RNA polymerase by α-P-boranophosphate nucleotides: exploring a potential strategy for mechanism-based HCV drug design. Antiviral Res. 98(2), 14452 (2013).
  3. Rohayem, J. et al. Antiviral strategies to control calicivirus infections. Antiviral Res. 87(2), 162-78 (2010).

価格表

製品名メーカー
製品番号
容量オンライン
カタログへ
4-Thiouridine-5'-triphosphateN-1025-11 µmolee-Nacalai.jpg
N-1025-55 µmolese-Nacalai.jpg
N-1025-1010 µmolese-Nacalai.jpg
Aracytidine-5'-triphosphateN-1033-11 µmolee-Nacalai.jpg
N-1033-55 µmolese-Nacalai.jpg
N-1033-1010 µmolese-Nacalai.jpg
Arauridine-5'-triphosphate*3N-1034-11 µmolee-Nacalai.jpg
N-1034-55 µmolese-Nacalai.jpg
N-1034-1010 µmolese-Nacalai.jpg
Araadenosine-5'-triphosphateN-1048-11 µmolee-Nacalai.jpg
N-1048-55 µmolese-Nacalai.jpg
N-1048-1010 µmolese-Nacalai.jpg
5-Fluoro-2'-deoxyuridine-5'-triphosphateN-2022-11 µmolee-Nacalai.jpg
N-2022-55 µmolese-Nacalai.jpg
N-2022-1010 µmolese-Nacalai.jpg
2',3'-Dideoxycytidine-5'-triphosphate*1N-4005-11 µmolee-Nacalai.jpg
N-4005-55 µmolese-Nacalai.jpg
N-4005-1010 µmolese-Nacalai.jpg
3'-Azido-2',3'-dideoxythymidine-5'-triphosphateN-4009-11 µmolee-Nacalai.jpg
N-4009-55 µmolese-Nacalai.jpg
N-4009-1010 µmolese-Nacalai.jpg
3'-Azido-2',3'-dideoxyuridine-5'-triphosphateN-4015-11 µmolee-Nacalai.jpg
N-4015-55 µmolese-Nacalai.jpg
N-4015-1010 µmolese-Nacalai.jpg
2',3'-Dideoxyinosine-5'-triphosphateN-4017-11 µmolee-Nacalai.jpg
N-4017-55 µmolese-Nacalai.jpg
N-4017-1010 µmolese-Nacalai.jpg
2'-Deoxyadenosine-5'-O-(1-boranotriphosphate)*2N-8050-11 µmolee-Nacalai.jpg
N-8050-55 µmolese-Nacalai.jpg
N-8050-1010 µmolese-Nacalai.jpg
Ganciclovir TriphosphateN-9001-11 µmolee-Nacalai.jpg
N-9001-55 µmolese-Nacalai.jpg
N-9001-1010 µmolese-Nacalai.jpg
Cidofovir-diphosphateN-9002-11 µmolee-Nacalai.jpg
N-9002-55 µmolese-Nacalai.jpg
N-9002-1010 µmolese-Nacalai.jpg

エピジェネティクス研究

DNA のメチル化を例とするエピゲノムは、がん、細胞の分化、記憶、記憶の形成、X 染色体の活性化などの細胞内の各種パスウェイにおいて鍵となる役割を担うと考えられています。TriLink 社ではエピジェネティクス研究に有用な修飾ヌクレオチドを取り扱っています。

5-Carboxymethylesteruridine-5'-triphosphate5-Hydroxymethyl-2'-deoxycytidine-5'-triphosphate
N-1096.png N-2060.png

使用文献(一部)

  1. Li, Z. et al. Gadd45a promotes DNA demethylation through TDG. Nucleic Acids Res. 43(8), 3986-97 (2015).
  2. Schwartz, S. et al. High-resolution mapping reveals a conserved, widespread, dynamic mRNA methylation program in yeast meiosis. Cell 155(6), 1409-21 (2013).
  3. Mellén, M. et al. MeCP2 binds to 5hmC enriched within active genes and accessible chromatin in the nervous system. Cell 151(7), 1417-30 (2012).

価格表

製品名メーカー
製品番号
容量オンライン
カタログへ
N6-Methyladenosine-5'-triphosphate*2N-1013-11 µmolee-Nacalai.jpg
N-1013-55 µmolese-Nacalai.jpg
N-1013-1010 µmolese-Nacalai.jpg
5-Carboxycytidine-5'-triphosphateN-1084-11 µmolee-Nacalai.jpg
N-1084-55 µmolese-Nacalai.jpg
N-1084-1010 µmolese-Nacalai.jpg
N-1084-505 X 10 µmolese-Nacalai.jpg
N-1084-10010 X 10 µmolese-Nacalai.jpg
5-Formylcytidine-5'-triphosphateN-1085-11 µmolee-Nacalai.jpg
N-1085-55 µmolese-Nacalai.jpg
N-1085-1010 µmolese-Nacalai.jpg
N-1085-5050 µmolese-Nacalai.jpg
N-1085-100100 µmolese-Nacalai.jpg
5-Hydroxycytidine-5'-triphosphateN-1089-11 µmolee-Nacalai.jpg
N-1089-55 µmolese-Nacalai.jpg
N-1089-1010 µmolese-Nacalai.jpg
N-1089-5050 µmolese-Nacalai.jpg
N-1089-100100 µmolese-Nacalai.jpg
5-Hydroxymethylcytidine-5'-triphosphateN-1087-11 µmolee-Nacalai.jpg
N-1087-55 µmolese-Nacalai.jpg
N-1087-1010 µmolese-Nacalai.jpg
N-1087-505 X 10 µmolese-Nacalai.jpg
N-1087-100100 µmolese-Nacalai.jpg
5-Methylcytidine-5'-triphosphateN-1014-11 µmolee-Nacalai.jpg
N-1014-55 µmolese-Nacalai.jpg
N-1014-1010 µmolese-Nacalai.jpg
5-Methoxycytidine-5'-triphosphateN-1094-11 µmolee-Nacalai.jpg
N-1094-55 µmolese-Nacalai.jpg
N-1094-1010 µmolese-Nacalai.jpg
N-1094-505 X 10 µmolese-Nacalai.jpg
N-1094-10010 X 10 µmolese-Nacalai.jpg
O6-Methylguanosine-5'-triphosphateN-1031-11 µmolee-Nacalai.jpg
N-1031-55 µmolese-Nacalai.jpg
N-1031-1010 µmolese-Nacalai.jpg
8-Oxoguanosine-5'-triphosphateN-1066-11 µmolee-Nacalai.jpg
N-1066-55 µmolese-Nacalai.jpg
N-1066-1010 µmolese-Nacalai.jpg
5-Carboxyuridine-5'-triphosphateN-1091-11 µmolee-Nacalai.jpg
N-1091-55 µmolese-Nacalai.jpg
N-1091-1010 µmolese-Nacalai.jpg
N-1091-505 X 10 µmolese-Nacalai.jpg
N-1091-10010 X 10 µmolese-Nacalai.jpg
5-Carboxymethylesteruridine-5'-triphosphateN-1096-11 µmolee-Nacalai.jpg
N-1096-55 µmolese-Nacalai.jpg
N-1096-1010 µmolese-Nacalai.jpg
N-1096-505 X 10 µmolese-Nacalai.jpg
N-1096-10010 X 10 µmolese-Nacalai.jpg
5-Formyluridine-5'-triphosphateN-1090-11 µmolee-Nacalai.jpg
N-1090-55 µmolese-Nacalai.jpg
N-1090-1010 µmolese-Nacalai.jpg
N-1090-505 X 10 µmolese-Nacalai.jpg
N-1090-10010 X 10 µmolese-Nacalai.jpg
5-Hydroxyuridine-5'-triphosphateN-1092-11 µmolee-Nacalai.jpg
N-1092-55 µmolese-Nacalai.jpg
N-1092-1010 µmolese-Nacalai.jpg
N-1092-505 X 10 µmolese-Nacalai.jpg
N-1092-10010 X 10 µmolese-Nacalai.jpg
5-Hydroxymethyluridine-5'-triphosphateN-1086-11 µmolee-Nacalai.jpg
N-1086-55 µmolese-Nacalai.jpg
N-1086-1010 µmolese-Nacalai.jpg
N-1086-505 X 10 µmolese-Nacalai.jpg
N-1086-10010 X 10 µmolese-Nacalai.jpg
5-Methoxyuridine-5'-triphosphateN-1093-11 µmolee-Nacalai.jpg
N-1093-55 µmolese-Nacalai.jpg
N-1093-1010 µmolese-Nacalai.jpg
N-1093-505 X 10 µmolese-Nacalai.jpg
N-1093-10010 X 10 µmolese-Nacalai.jpg
N6-Methyl-2'-deoxyadenosine-5'-triphosphateN-2025-11 µmolee-Nacalai.jpg
N-2025-55 µmolese-Nacalai.jpg
N-2025-1010 µmolese-Nacalai.jpg
O6-Methyl-2'-deoxyguanosine-5'-triphosphateN-2027-11 µmolee-Nacalai.jpg
N-2027-55 µmolese-Nacalai.jpg
N-2027-1010 µmolese-Nacalai.jpg
8-Oxo-2'-deoxyguanosine-5'-triphosphateN-2034-11 µmolee-Nacalai.jpg
N-2034-55 µmolese-Nacalai.jpg
N-2034-1010 µmolese-Nacalai.jpg
N4-Methyl-2'-deoxycytidine-5'-triphosphateN-2057-11 µmolee-Nacalai.jpg
N-2057-55 µmolese-Nacalai.jpg
N-2057-1010 µmolese-Nacalai.jpg
5-Methyl-2'-deoxycytidine-5'-triphosphate*3N-2026-11 µmolee-Nacalai.jpg
N-2026-55 µmolese-Nacalai.jpg
N-2026-1010 µmolese-Nacalai.jpg
5-Hydroxymethyl-2'-deoxycytidine-5'-triphosphate*1, 3N-2060-11 µmolee-Nacalai.jpg
N-2060-55 µmolese-Nacalai.jpg
N-2060-1010 µmolese-Nacalai.jpg
5-Carboxy-2'-deoxycytidine-5'-triphosphate*1N-2063-11 µmolee-Nacalai.jpg
N-2063-55 µmolese-Nacalai.jpg
N-2063-1010 µmolese-Nacalai.jpg
5-Formyl-2'-deoxycytidine-5'-triphosphateN-2064-11 µmolee-Nacalai.jpg
N-2064-55 µmolese-Nacalai.jpg
N-2064-1010 µmolese-Nacalai.jpg
5-Carboxy-2'-deoxyuridine-5'-triphosphateN-2066-11 µmolee-Nacalai.jpg
N-2066-55 µmolese-Nacalai.jpg
N-2066-1010 µmolese-Nacalai.jpg
5-Hydroxymethyl-2'-deoxyuridine-5'-triphosphateN-2059-11 µmolee-Nacalai.jpg
N-2059-55 µmolese-Nacalai.jpg
N-2059-1010 µmolese-Nacalai.jpg

その他(遺伝子治療研究、再生医療研究など)

修飾ヌクレオチドを用いた mRNA は免疫原性が低く、遺伝子発現効率の向上も可能なことから、遺伝子治療研究や再生医療研究などさまざまな研究分野で応用されています。

5-Methylcytidine-5'-triphosphatePseudouridine-5'-triphosphate
N-1014.pngN-1019.png

使用文献(一部)

  1. Lee, K. et al. Peptide-enhanced mRNA transfection in cultured mouse cardiac fibroblasts and direct reprogramming towards cardiomyocyte-like cells. Int J Nanomedicine. 10, 1841-54 (2015).
  2. Uchida, S. et al. Screening of mRNA chemical modification to maximize protein expression with reduced immunogenicity. Pharmaceutics 7(3), 137-51 (2015).
  3. Wang, Y. et al. Systemic delivery of modified mRNA encoding herpes simplex virus 1 thymidine kinase for targeted cancer gene therapy. Mol Ther. 21(2), 358-67 (2013).
  4. Warren, L. et al. Highly efficient reprogramming to pluripotency and directed differentiation of human cells with synthetic modified mRNA. Cell Stem Cell 7(5), 618-30 (2010).

価格表

製品名メーカー
製品番号
容量オンライン
カタログへ
N6-Methyladenosine-5'-triphosphateN-1013-11 µmolee-Nacalai.jpg
N-1013-55 µmolese-Nacalai.jpg
N-1013-1010 µmolese-Nacalai.jpg
5-Methylcytidine-5'-triphosphate*3,4N-1014-11 µmolee-Nacalai.jpg
N-1014-55 µmolese-Nacalai.jpg
N-1014-1010 µmolese-Nacalai.jpg
Pseudouridine-5'-triphosphate*2, 3, 4N-1019-11 µmolee-Nacalai.jpg
N-1019-55 µmolese-Nacalai.jpg
N-1019-1010 µmolese-Nacalai.jpg
2-Thiouridine-5'-triphosphate*2N-1032-11 µmolee-Nacalai.jpg
N-1032-55 µmolese-Nacalai.jpg
N-1032-1010 µmolese-Nacalai.jpg
N1-Methylpseudouridine-5'-triphosphateN-1081-11 µmolee-Nacalai.jpg
N-1081-55 µmolese-Nacalai.jpg
N-1081-1010 µmolese-Nacalai.jpg
Thienoguanosine-5'-triphosphateN-1088-11 µmolee-Nacalai.jpg
N-1088-55 µmolese-Nacalai.jpg
N-1088-1010 µmolese-Nacalai.jpg
N-1088-5050 µmolese-Nacalai.jpg
N-1088-100100 µmolese-Nacalai.jpg
ARCA(Anti Reverse Cap Analog)*1, 3, 4N-7003-11 µmolee-Nacalai.jpg
N-7003-55 µmolese-Nacalai.jpg
N-7003-1010 µmolese-Nacalai.jpg
Immune Stimulation Reduction Transcription Nucleotide Set
#N-1014(1.0 µmole), #N-1019(1.0 µmole), #N-7003(2 X 1.0 µmole)
K-10181 sete-Nacalai.jpg
※掲載内容は予告なく変更になる場合があります。