Epitope Tag Antibody

Anti-GFP(Rat lgG2a), Monoclonal(GF090R)

Feature
  • Immunohistochemical application
  • Rat monoclonal antibody
Clone GF090R
GF090R IgG2a(Rat)
Product form Liquid
Immunogen His-GFP (full length) fusion protein
Application Immunohistochemstry 1:1000 - 1:2000
Western blotting 1:1000 - 1:2000
ELISA 1:2000 - 1:20000

Immunocytochemistry

 GFP_naka  GFP_migi
Sample Mouse brain (nerve cell)
Primary antibody Anti-GFP(Rat IgG2a), Monoclonal (1:1000) RT, O/N
Secondary antibody Anti-Rat IgG-Cy3 (1:300) RT, 1hr
Blocking 5% normal goat serum / 0.2% TritonX-100 in PBS
Fixing method 4% Paraformaldehyde

Data courtesy of Dr.Yoshihara Y., RIKEN Brain Science Institute

Western blotting

Anti-GFP_WB
Sample Lane 1) GFP
Lane 2) GFP-14-3-3
(293 cell lysate 50 μg)
Gel 5-20% Gradient Gel
1st antibody Anti-GFP(Rat IgG2a), Monoclonal (3 μg/ml) O/N
2nd antibody Anti-Rat IgG-HRP(1:10000)
Data courtesy of Dr.Yoshida K., Department of Molecular Genetics,Medical Research Institute, Tokyo Medical and Dental University

Reference List

for Immunocytochemistry / Immunohistochemistry

for Western Blotting and Immunoblotting

Ordering Information

Product name Application Storage Code No. Quantily  Price
Anti-GFP (Rat IgG2a), Monoclonal (GF090R) WB, IHC, ELISA R 04404-26 50 µg Inquire
04404-84 200 µg Inquire

(Storage) R= Refrigerator

Anti-GFP (Rat IgG2a), Mono (GF090R),CC, Agarose Conjugate

Western blotting

GFP_Conjugate

Samples: HEK-293 cell lysate expressing either EGFP or EGFPMyosin(1 ml each)
Immunoprecipitation was performed with Anti-GFP agarose conjugate (20 µl) at 4°C for 2 hours.

Data courtesy of Dr. A. Kakizuka, Laboratory of Functional Biology, Graduate School of Biostudies, Kyoto University

Ordering Information

Product name Application Storage Code No. Quantily  Price
Anti-GFP (Rat IgG2a), Monoclonal(GF090R), CC, Agarose Conjugate IP R 06083-05 500 µg Inquire

(Storage) R= Refrigerator

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For research use only, not intended for diagnostic or drug use.

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