NACALAI TESQUE, INC.

SUMO Expression Systems

SUMO-based Protein and Peptide Expression Systems maximise the yield of soluble, functional proteins in E. coli, Yeast, Insect cell, and Mammalian cell cultures. SUMO acts partially like a chaperonin and partially as an initiator of protein folding to dramatically improve the solubility of the protein of interest when expressed at high levels. LifeSensors desumoylases efficiently and robustly remove the SUMO tag exactly at the junction of the C-terminus of SUMO and the N-terminus of your protein to release it with the N-terminal amino acid you desire (with the exception of proline). Both SUMO and the desumoylases have hexaHis tags making their subsequent removal fast and easy. Currently, LifeSensors’ SUMO-tags come in three flavors: SUMOpro, SUMOpro-3, and SUMOstar. For more information, visit www.lifesensors.com.

SUMO expression technology

• LifeSensors’ technology (PDF file)
• SUMO Vector Table (PDF file)
• SUMO Star (PDF file)

Application

• SUMOpro fusion enhance expression and solubility in E.coli
• SUMOstarTM Gene Fusion Technology in Insect Cells (PDF file)
• SUMOstarTM Gene Fusion Technology for Secretory Expression in Mammalian Cells (PDF file)

Press Release

Reference