Recombinant LIF Proteins
Description |
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Leukemia Inhibitory Factor (LIF) is a lymphoid factor that promotes long-term maintenance of pluripotent embryonic stem cells by suppressing spontaneous differentiation. Recombinant Mouse and Human Leukemia inhibitory factors (mLIF/hLIF) are produced in E. coli. They contain a single non-glycosylated polypeptide chain 181 amino acids and have a molecular mass of 20kDa. The mLIF/hLIF are purified by proprietary techniques using HPLC and FPLC® chromatography.
Application
Cell culture of mouse ES (CGR8)
| Recombinant Mouse LIF | A company |
|---|---|
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Recombinant Mouse LIF shows the same colony forming compared to A company's product.
Detection of undifferentiated markers（Nanog）
| Nanog（Alexa Fluor®488) | Nucleus（DAPI） |
|---|---|
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Nanog is detected in most of cells.
Data courtesy of Teruhisa Kawamura, MD, PhD, Career-Path Prootion Unit For Young Life Scientists , Kyoto UniversitySDS PAGE of mLIF Sample
| Figure 1. Recombinant Mouse LIF | Figure 2. Recombinant Human LIF |
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Specification
| Quality evaluation | Bioavtivity | approx. 108 units/mg (tested by the M1 cell differentiation assay) | |
| Undifferentiated state preservation | 1000 units/ml (tested by mouse ES cell) | Not tested | |
| Source | Escherichia Coli. | ||
| Purity （SDS-PAGE） | Greater than 99%, as determined by SDS-PAGE (See Fig. 1) | Greater than 95%, as determined by SDS-PAGE (See Fig. 2) | |
| Formulation | 0.22 µm filtered sterile liquid, phosphate-buffered saline with 0.02% Tween-20 and 1% BSA. | ||
| Storage | Maintain at 4°C for 12 months. Freeze-thaw should be avoided as it results in loss of activity. | ||
| Recommended concentration |
107 units, identical 100 µg of pure protein, are sufficient to treat 10.0 L of ES cell. | 0.5x107 units, identical 50 µg of pure protein, are sufficient to treat 5.0 L of stem cells including human embryonic stem cells, neural stem cells, hematopoietic stem cells, mesenchymal stem cells and induced pluripotent stem cells. | |
| Recombinant Mouse LIF | Recombinant Human LIF | ||
|---|---|---|---|
Ordering information
| Product name | Storage | Product No. | PKG Size |
|---|---|---|---|
| Recombinant Mouse LIF | 4°C | NU0012-1 | 1.0 ml （106 units/ml） |
| NU0012-2 | 1.0 ml （107 units/ml） | ||
| Recombinant Human LIF | 4°C | NU0013-1 | 1.0 ml （106 units/ml） |
| NU0013-2 | 1.0 ml （0.5×107 units/ml） |
Recombinant LIF Proteins are produced by Nacalai USA, Inc.
FPLC® is a registered trademark of GE Healthcare.
Alexa Fluor® is a registered trademark of Life Technologies.
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