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Urine Exosome Purification Kit

 

The Urine Exosome Purification Kits provide a fast, reliable and con- venient method to purify and enrich for pure intact exosomes from different urine sample volumes ranging from 250 µL to 30 mL. These kits also allow for the purification of intact extracellular vesicles (EVs) from different urine sample volumes, and these EVs are ready for any downstream application. The purification is based on Norgen’s proprietary resin.

These kits provide a clear advantage over other available methods since they do not require any special instrumentation, ultracentrifugation, precipitation reagents or any protease treatments. More importantly, the purified exosomes will not be contaminated with any　other RNA-binding proteins that may contaminate your exosomal RNA, which is essential if studying exosomal RNA gene expression.

Exosomal RNA can be isolated using Norgen’s Exosomal RNA Isolation Kit from exosomes enriched using Norgen’s Urine Exo- some Purification Kits for gene expression analysis using RT-qPCR, microarray or NGS and for Biomarker discovery.

 

Kit Specifications
Urine Input (Cat. 57700)	250 μL - 1 mL	Size of Exosomes Purified	40 nm - 150 nm
Urine Input (Cat. 57800)	2 mL - 10 mL	Elution Volume	Variable depending on the urine input volume
Urine Input (Cat. 57900)	11 mL - 30 mL	Time to Complete 10 Purifica- tions	15 - 30 minutes

 

 

Features and Benefits
Diverse	Purification and enrichment of intact urinary exosomes for functional studies; compatible with urine from any species
Versatile	Versatile sample input ranging from 250 µL to 30 mL
Phenol-Free	No phenol extractions nor carrier RNA
Compatible with various platforms	Purified exosomes can be analyzed using NanoSight® or Electron Micros- copy for assessing the approximate exosome size range and concentra- tion
Fast and Accurate	No time-consuming ultracentrifugation, filtration nor special syringes re- quired; Purify high-quality RNA/DNA in 15 - 30 minutes

 

 

Figure 1. Intact exosomes purified from 5 mL using different purification methods. Intact exosomes were purified from 5 mL urine using Norgen's Urine Exosome Purification Midi Kit (Cat# 57800), Competitor S's kit, and Competitor L's kit. Exosomes purified using Norgen's kit were resuspended in 400 µL Norgen's ExoR buffer, diluted 1:1,000 and visualized on the NanoSight LM10 instrument. The analysis shows that Norgen's kit isolated 115 nm exosomes with a recovery of 8.36 x 109 particles/mL urine samples. No impurities were found to be contaminating the exosomes purified using Norgen's Urine Exosome Purification Midi Kit. Additionally, Exosomes with a broader size range covering from 75nm - 250nm were purified from 5 mL urine with a higher concentration as compared to the other two methods.

 

 

 

 

Figure 2. Intact Exosomes were purified from 10 mL urine using Norgen's Urine Exosome Purification Midi Kit (Cat# 57800) and Ultracentrifugation. Exosomes purified using Norgen's kit and ultracentrifugation were resuspended in 400 µL of Norgen's ExoR buffer, diluted 1:1,000 and visualized on the NanoSight LM10 instrument. The analysis shows that Norgen's kit purified exosomes with sizes ranging from 65 nm to 195 nm, with a total recovery of 7.63 x 108particles/mL. No impurities were found to be contaminating the exosomes purified using Norgen's Urine Exosome Purification Midi Kit as opposed to the exo- somes purified using ultracentrifugation, which purified exosomes with larger particle sizes ranging from 125 nm - 235 nm with a total recovery of 1.56 x 108 particles/mL.

 

 

 

Component	Cat. 57700	Cat. 57800	Cat. 57900
Slurry E	12.5 mL	12.5 mL	12.5 mL
ExoC Buffer	8 mL	30 mL	50 mL
ExoR Buffer	12 mL	12 mL	12 mL
Mini Filter Spin Columns	50	25	15
Elution Tubes (1.7 mL)	50	25	15
Product Insert	1	1	1

 

 

 

Storage Conditions

All buffers should be kept tightly sealed and stored at room temperature (15-25°C) for up to 2 years without showing any reduction in performance.

 

 

 

Important Note

Urine samples stored at -70°C, -20°C or at 4°C will develop some precipitation due to the aggregation of some of the highly abundant proteins in urine. Eliminating these precipi- tates using centrifugation or filtration may cause the loss of exosomes. Furthermore, these precipitates may affect the quality of the purified nucleic acid. We recommend the use of Norgen’s Urine Preservative when collecting urine sam- ples, which is designed for the preservation of nucleic acids and proteins in fresh urine samples at ambient temperatures. The components of the Urine Preservative allow samples to be stored for over 2 years at room temperature with no de- tected degradation of urine DNA, RNA or proteins. Norgen’s Urine Preservative is available as a liquid format in Norgen’s Urine Preservative Single Dose Ampules, as well as in a dried format in Norgen’s Urine Collection and Preservation Tubes.

 

 

 

Customer-Supplied Reagents and Equipment

• Disposable powder-free gloves
• Centrifuge with a swinging bucket rotor capable of 2,000 RPM
• Benchtop microcentrifuge
• Micropipettors
• Sterile pipette tips with filters
• 1.5 mL tubes
• 15 mL conical tubes
• 50 mL conical tubes
• Nuclease-Free Water 

 

 

Cat #	Description	Size
57700	Urine Exosome Purification Mini Kit	50 preps
57800	Urine Exosome Purification Midi Kit	25 preps
57900	Urine Exosome Purification Maxi Kit	15 preps

 

Norgen Web page: https://norgenbiotek.com/product/urine-exosome-purification-kit#

 

 

 

この製品に関するご意見・ご照会・お問合せはこちら