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HemoGenix社 ヒト幹細胞・前駆細胞増殖測定キット

細胞内ATPを測定

Hemogenix.JPG

 

 

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HALO®-96 HuSPCA Applications:
  • For all human lympho-hematopoietic research applications.
  • Detect and measure proliferation of human stem and progenitor cells.
  • Measure and compare proliferation status and/or potential between human tissues and cell populations.
  • Experimental transplantation models.
  • Effects of growth factors and/or cytokines.
  • In vivo to in vitro assays.
  • Gene targeting assays.
  •  

    Why Use HALO®-96 HuSPCA for Your Research?:
  • HALO®-96 HuSPCA is the only assay system available to detect and measure lympho-hematopoietic proliferation of human cells. (The colony-forming cell, CFC, does not measure proliferation; it is a differentiation assay because colonies are identified by the differentiation and maturation of the cells that form the colonies. Please see CAMEO™-4 and CAMEO™-96). See HALO® and CFC Information Flyer. 
  • Distinguish between multiple primitive and mature stem cell populations and lineage-specific populations.
  • Reliably compare your results from one experiment to another and over time by calibrating and standardizing the assay (reagents provided).
  • Provides all the flexibility you need for your research, including adding your own growth factors and/or cytokines.
  • HALO® utilizes Suspension Expansion Culture (SEC) and bioluminomics™ Technology that can be easily multiplexed with other assay readouts to obtain the most information from the same sample.
  • The following proliferation status or potential can be detected for individual populations using HALO®-96 HuSPCA:

    Stem Cells

    • HPP-SP 1: IL-3, IL-6, SCF, TPO, Flt3-L

    HPP-SP 2: EPO, GM-CSF, IL-2, IL-3, Il-6, IL-7, SCF, TPO, Flt3-L

    CFC-GEMM 1: EPO, GM-CSF, IL-3, IL-6, SCF, TPO, Flt3-L

    CFC-GEMM 2: EPO, GM-CSF, IL-3, IL-6, SCF, TPO

    CFC-GEM 1: EPO, GM-CSF, IL-3, IL-6, SCF

    CFC-GEM 2: EPO, GM-CSF, IL-3, SCF

    CFC-GEM 3: EPO, GM-CSF, G-CSF, IL-3, SCF

    Progenitor Cells

    BFU-E 1: EPO, IL-3, SCF

    BFU-E 2: EPO

    GM-CFC 1: GM-CSF, IL-3, SCF

    GM-CFC 2: GM-CSF, G-CSF, IL-3, SCF

    GM-CFC 3: GM-CSF

    Mk-CFC 1: TPO, IL-3, SCF

    Mk-CFC 2: TPO

    Lymphopoietic cells

    T-CFC: IL-2

    B-CFC: IL-7

    No growth factors: Add growth factors/cytokines of choice.

     

    Multiple Human Cell Populations Detected Simultaneously Using HALO®-96 HuSPCA:

    • 4-Population Assay

    • CFC-GEMM 1

    • BFU-E 1

    • GM-CFC 1

    • Mk-CFC 1

    • 7-Population Assay

    • HPP-SP 2

    • CFC-GEMM 1

    • BFU-E 1

    • GM-CFC 1

    • Mk-CFC 1

    • T-CFC

    •B-CFC

    +Background control

    Example of a 7-Population Comparison Between Human Tissues

    The graph shows the response of 7 lympho-hematopoietic cell populations derived from mobilized peripheral blood CD34+ cells, bone marrow and umbilical cord blood mononuclear cells. Please note that whereas mobilized peripheral blood CD34+ cells are shown at 500 CD34+/well, bone marrow and umbilical cord blood are shown at 5,000 mononuclear cells/well.

     

     

     

    cellcountingfig.JPG

     

     

    Use:

    For Research Use Only. Not for clinical diagnostic use

     

    Kit Contents:

    • HALO®-96 HuSPCA Master Mix

    • ATP standard

    • ATP high and low controls (not included with research kits)

    • ATP Enumeration Reagent

    • Sterile, 96-well plates

    • Non-sterile, 96-well plates

    • Sterile, adhesive foil covers

    • Instruction manual

     

    Product Name

    Product No.

    Cell Population

    Species

    Size

    HALO®-96 Hu SPCA

    K2-HPP1-1H

    HPP-SP1

    Human

    1x96-well plate

    HALO®-96 Hu SPCA

    K2-HPP2-1H

    HPP-SP2

    Human

    1x96-well plate

    HALO®-96 Hu SPCA

    K2-GEMM1-1H

    CFC-GEMM 1

    Human

    1x96-well plate

    HALO®-96 Hu SPCA

    K2-GEMM2-1H

    CFC-GEMM 2

    Human

    1x96-well plate

    HALO®-96 Hu SPCA

    K2-GEM1-1H

    CFC-GEM 1

    Human

    1x96-well plate

    HALO®-96 Hu SPCA

    K2-GEM2-1H

    CFC-GEM 2

    Human

    1x96-well plate

    HALO®-96 Hu SPCA

    K2-GEM3-1H

    CFC-GEM 3

    Human

    1x96-well plate

     

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