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SinoBiological社 IL-2: A Cytokine with Immunostimulatory and Immunoregulatory Functions

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IL-2_A_Cytokine_with_Immunostimulatory_and_Immunoregulatory_Functions.jpgInterleukin 2 (IL-2) is a pleiotropic cytokine central to the regulation of T-cell responses. In addition, IL-2 plays critical roles in a wide range of other immune modulatory actions.

Interleukin_2(IL-2).pngIL-2 mediates its function by binding to IL-2 receptors (IL-2R). There are three subunits of IL-2 receptor, α chain (CD25), β chain (CD122), and γ chain (CD132). The β and γ chains are shared between IL-2 and IL-15 receptor complexes, while the γ chain is also used by IL-4, IL-7, IL-9, and IL-21. IL-2 binds to IL-2Rα alone with low affinity, to IL-2Rβ and IL-2Rγ heterodimer with intermediate affinity, and to IL-2α/β/γ heterotrimer with high affinity. Expression of IL-2R by different immune cell subsets determines the cellular sensitivity to IL-2. IL-2 signaling is then transduced via three different pathways: JAK-STAT, PI3K/Akt/mTOR, and MAPK/ERK.

Recent studies have recognized IL-2/IL-2R signaling pathway as an important target for the treatment of various diseases, including cancer, infectious diseases, and autoimmune diseases. New strategies are now being developed to improve the efficacy and lower toxic effects of IL-2 therapy. Sino Biological has developed a panel of recombinant IL-2 and IL-2R protein reagents to facilitate the study of the biology of IL-2.

 

Recombinant IL-2 Proteins

In addition to therapeutic usage, recombinant IL-2 protein also allows the culture of IL-2 dependent T-cell lines and NK cell lines, the proliferation of mitogen-activated T cells and NK cells, the establishment of thymocyte, splenocyte or peripheral blood lymphocyte derived T-cell lines, and the generation of lymphokine-activated killer (LAK) cells.

Sino Biological has developed high activity IL-2 proteins from various species. The activities of these proteins have been validated by cell proliferation assays using CTLL2 cells.

Human IL-2
(11848-H08H)

Human IL-2
(11848-HNAY1)

Human_IL-2_(11848-H08H).png

Human IL-2_(11848-HNAY1).png

Cell proliferation assay using CTLL2 cells.
The ED50 is typically 1-5 ng/mL.

Cell proliferation assay using CTLL2 cells.
The ED50 is 0.35-1.4 ng/mL.

51061-MNAE.png

51061-MNAE

 

70014-DNAE.png

70014-DNAE

 

80075-RNAE.png

80075-RNAE

 

 

Recombinant IL-2 Receptor Proteins

IL-2 receptors with different subunits have been developed to accelerate the research of IL-2/IL-2R signaling.

Cat#

Species

Receptor

Expressed Host

Tag

Activity Validated

10165-H02H

Human

IL-2Rα

HEK293

hFc

Bind to human IL-2

10165-H08H

Human

IL-2Rα

HEK293

His

Inhibit IL-2 induced proliferation

50292-M02H

Mouse

IL-2Rα

HEK293

hFc

 

80074-R08B

Rat

IL-2Rα

Insect

His

 

90265-C02H

Cynomolgus

IL-2Rα

HEK293

hFc

 

90265-C27H-B

Rhesus

IL-2Rα

HEK293

AVI & His

 

10696-H05H

Human

IL-2Rβ

HEK293

mFc

Bind to human IL-2

50792-M08H

Mouse

IL-2Rβ

HEK293

His

 

70082-D02H

Canine

IL-2Rβ

HEK293

hFc

 

10555-H08H

Human

IL-2Rγ

HEK293

His

 

10555-H08B

Human

IL-2Rγ

Insect

His

Bind to rat IL-2

50087-M03H

Mouse

IL-2Rγ

HEK293

Fc & His

 

80197-R02H

Rat

IL-2Rγ

HEK293

hFc

 

For full list, please kindly refer to www.sinobiological.com

 
Cytokines Share the IL-2R γ Chain
The cytokines that share the common gamma-chain (γc) among their receptor subunits including interleukin-2 (IL-2), IL-4, IL-7, IL-9, IL-15, and IL-21, play dominant roles in the regulation of immune responses.
Among members of γc family, IL-15 shares many biological activities to IL-2 as they bind common hematopoietic receptor (IL-2/15Rβ & γc) subunits and may compete for the same receptor. IL-15 induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators, STAT3, STAT5, and STAT6. 

IL-15

 

IL-4

 

IL-7

 

IL-9

 

IL-21

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