PBL's VeriKineTM ELISA kits are heterogeneous antibody sandwich immunoassays. Interferon is captured by the antibody immobilized to microtiter plate wells, and a detection antibody is used to complete the sandwich. A signal generator of conjugated horseradish peroxidase (HRP), together with tetramethyl benzidine (TMB), is used to develop the assay. To address the need to detect different human alpha interferones, PBL offers the three types ofVeriKineTM Human IFN Alpha ELISA kits.
Compatison ofVeriKineTMHuman IFN Alpha ELISA kits.
Interferon | Gene | Detection Sensitivity | ||
---|---|---|---|---|
#41100 Alpha A |
#41105 Multi-Subtype |
#41110 Multi-Subtype Serum |
||
Human IFN-αA | IFNA2 | ++ | ++ | ++ |
Human IFN-α2 | IFNA2 | ++ | ++ | ++ |
Human IFN-αA/D | - | ++ | ++ | ++ |
Human IFN-αB2 | IFNA8 | - | ++ | ++ |
Human IFN-αC | IFNA10 | + | ++ | ++ |
Human IFN-αD | IFNA1 | ++ | ++ | ++ |
Human IFN-αF | IFNA21 | - | - | - |
Human IFN-αG | IFNA5 | + | ++ | ++ |
Human IFN-αH | IFNA14 | - | ++ | ++ |
Human IFN-αI | IFNA17 | - | ++ | ++ |
Human IFN-αJ1 | IFNA7 | + | ++ | ++ |
Human IFN-αK | IFNA6 | ++ | ++ | ++ |
Human IFN-α1 | IFNA1 | ++ | ++ | ++ |
Human IFN-α4a | IFNA4 | + | + | + |
Human IFN-α4b | IFNA4 | ++ | ++ | ++ |
Human IFN-αWA | IFNA16 | + | ++ | ++ |
Human IFN-β | - | - | - | - |
Human IFN-γ | - | - | - | - |
Human IFN-ω | - | - | - | - |
++; Very Good, +; Moderate, -; Not at all
Product name | Matrix Compatibility |
Assay Range | Product number | Size |
---|---|---|---|---|
VeriKineTM Human IFN-α ELISA | Cell Culture Supernatant | 12.5-500pg/ml or 156-5000pg/ml |
41100-1 41100-2 |
1 Plate 5 Plate |
VeriKineTM Human IFN-α Multi-Subtype ELISA |
Cell Culture Supernatant | 12.5-500pg/ml or 156-5000pg/ml |
41105-1 41105-2 |
1 Plate 5 Plate |
VeriKineTM Human IFN-α Multi-Subtype, Serum Samples ELISA |
Cell Culture Supernatant, Serum, Plasma | 12.5-500pg/ml or 156-5000pg/ml |
41110-1 41110-2 |
1 Plate 5 Plate |
VeriKineTM Human IFN-β ELISA | Cell Culture Supernatant | 25-2000pg/ml | 41410-1 41410-2 |
1 Plate 5 Plate |
VeriKineTM Human IFN-ω ELISA | Cell Culture Supernatant, Serum, Plasma | 3-500pg/ml | 41395-1 | 1 Plate |
VeriKineTM Human IFN-γ ELISA | Cell Culture Supernatant | 12.5-500pg/ml | 41500-1 | 1 Plate |
Product name | Matrix Compatibility |
Assay Range | Product number | Size |
---|---|---|---|---|
VeriKineTM Mouse IFN-α ELISA | Cell Culture Supernatant | 12.5-400pg/ml | 42120-1 42120-2 |
1 Plate 5 Plate |
VeriKineTM Mouse IFN-β ELISA | Cell Culture Supernatant, Serum |
1.5-1000pg/ml | 42400-1 42400-2 |
1 Plate 5 Plate |