COSMOSIL

RNA 分離用カラム

  • 1,000 塩基などの比較的大きな核酸分離に最適

ラインアップ

長鎖の核酸分離用カラムとして、「RNA-RP1」、「RNA-SEC-1000」および「RNA-SEC-2000」をラインアップしました。「RNA-RP1」は逆相クロマトグラフィー用カラム、一方、「RNA-SEC-1000」「RNA-SEC-2000」はゲルろ過クロマトグラフィー用カラムであり、異なる 2 種類の分離モードを取りそろえることで、mRNA のような長鎖核酸の分離分析への活用が期待されます。

RNA 分離用充填剤の物性一覧表

充填剤名称RNA-RP1RNA-SEC-1000RNA-SEC-2000
分離モード 逆相クロマトグラフィー ゲルろ過クロマトグラフィー
シリカゲル 全多孔性球状高純度シリカゲル
平均粒子径(µm) 5
平均細孔径(約 Å) 1,000 2,000
化学結合基 オクタデシル基 親水性基
使用可能 pH 範囲 2 ~ 7.5
使用圧力(MPa 以下) 15

分析例

RNA-RP1 カラム

ssRNA Ladder(1)

AP-1870.png

Conditions
Column COSMOSIL RNA-RP1
4.6 mm I.D. × 100 mm
Temperature 65℃
Mobile phase A; 100 mmol/L Triethylamine, 100 mmol/L Acetic Acid (pH 7)
B; Acetonitrile
B conc. 5 → 20% (0 → 20 min), 20% (20 - 24 min)
Detection UV 260 nm
Sample ssRNA Ladder, Low Range (200 ng/µL)
Flow rate 1.0 mL/min Inj. Vol. 5.0 µL

ssRNA Ladder(2)

AP-1871.png

Conditions
Column COSMOSIL RNA-RP1
4.6 mm I.D. × 100 mm
Temperature 65℃
Mobile phase A; 100 mmol/L Triethylamine, 100 mmol/L Acetic Acid (pH 7)
B; Acetonitrile
B conc. 8 → 13% (0 → 20 min), 13% (20 - 24 min)
Detection UV 260 nm
Sample ssRNA Ladder (200 ng/µL)
Flow rate 1.0 mL/min Inj. Vol. 5.0 µL

dsRNA Ladder

AP-1869.png

Conditions
Column COSMOSIL RNA-RP1
4.6 mm I.D. × 100 mm
Temperature 40℃
Mobile phase A; 100 mmol/L Triethylamine, 100 mmol/L Acetic Acid (pH 7)
B; Acetonitrile
B conc. 5 → 20% (0 → 20 min), 20% (20 - 24 min)
Detection UV 260 nm
Sample dsRNA Ladder (200 ng/µL)
Flow rate 1.0 mL/min Inj. Vol. 5.0 µL

ssRNA Ladder、dsRNA Ladder の同時分析

AP-RP1-1.png

Conditions
Column COSMOSIL RNA-RP1
2.0 mm I.D. × 100 mm
Temperature 60℃
Mobile phase A; 100 mmol/L Triethylamine, 100 mmol/L Acetic Acid (pH 7)
B; Acetonitrile
B conc. 5 → 20% (0 → 20 min), 20% (20 - 24 min)
Detection UV 260 nm
Sample ssRNA Ladder
dsRNA Ladder
Flow rate 0.2 mL/min Inj. Vol. 2.0 µL

mRNA

AP-RP1-2.png

Conditions
Column COSMOSIL RNA-RP1
2.0 mm I.D. × 100 mm
Temperature 60℃
Mobile phase A; 100 mmol/L Triethylamine, 100 mmol/L Acetic Acid, 20 mmol/L Phosphate Buffer (pH 7.0)
B; Acetonitrile
B conc. 8 → 11% (0 → 20 min), 11% (20 - 24 min)
Detection UV 260 nm
Sample QBIT7-GFP RNA (4.8 knt) (100 ng/µL)
Flow rate 0.2 mL/min Inj. Vol. 5.0 µL

DNA Ladder

AP-1864.png

Conditions
Column COSMOSIL RNA-RP1
4.6 mm I.D. × 100 mm
Temperature 40℃
Mobile phase A; 100 mmol/L Triethylamine, 100 mmol/L Acetic Acid (pH 7)
B; Acetonitrile
B conc. 12 → 18% (0 → 20 min), 18% (20 - 25 min)
Detection UV 260 nm
Sample 100 bp DNA Ladder (130 ng/µL)
Flow rate 1.0 mL/min Inj. Vol. 5.0 µL

pBR322/MspI digest

AP-1868.png

Conditions
Column COSMOSIL RNA-RP1
4.6 mm I.D. × 100 mm
Temperature 40℃
Mobile phase A; 100 mmol/L Triethylamine, 100 mmol/L Acetic Acid (pH 7)
B; Acetonitrile
B conc. 10 → 17% (0 → 20 min), 17% (20 - 25 min)
Detection UV 260 nm
Sample pBR322/MspI digest (200 ng/µL)
Flow rate 1.0 mL/min Inj. Vol. 5.0 µL
その他の分析例はこちら

RNA-SEC-1000、2000 カラム

ssRNA Ladder(1)

AP-1860.png

Conditions
Column COSMOSIL RNA-SEC-****
4.6 mm I.D. × 250 mm
Temperature 65℃
Mobile phase 20 mmol/L Phosphate Buffer (pH 7.0) Detection UV 260 nm
Sample ssRNA Ladder, Low Range (200 ng/µL)
Flow rate 0.3 mL/min Inj. Vol. 2.0 µL

ssRNA Ladder(2)

AP-1861.png

Conditions
Column COSMOSIL RNA-SEC-****
4.6 mm I.D. × 250 mm
Temperature 65℃
Mobile phase 20 mmol/L Phosphate Buffer (pH 7.0) Detection UV 260 nm
Sample ssRNA Ladder (200 ng/µL)
Flow rate 0.3 mL/min Inj. Vol. 2.0 µL

dsRNA Ladder

ap-1859.png

Conditions
Column COSMOSIL RNA-SEC-****
4.6 mm I.D. × 250 mm
Temperature 65℃
Mobile phase 20 mmol/L Phosphate Buffer (pH 7.0) Detection UV 260 nm
Sample dsRNA Ladder (200 ng/µL)
Flow rate 0.3 mL/min Inj. Vol. 2.0 µL

ssRNA Ladder、dsRNA Ladder

ap-1862.png

Conditions
Column COSMOSIL RNA-SEC-1000
4.6 mm I.D. × 250 mm
Temperature 65℃
Mobile phase 20 mmol/L Phosphate Buffer (pH 7.0) Detection UV 260 nm
Sample ssRNA Ladder, Low Range (200 ng/µL)
dsRNA Ladder (200 ng/µL)
Flow rate 0.3 mL/min Inj. Vol. 2.0 µL

DNA Ladder

ap-1853.png

Conditions
Column COSMOSIL RNA-SEC-****
4.6 mm I.D. × 250 mm
Temperature 40℃
Mobile phase 20 mmol/L Phosphate Buffer (pH 7.0) Detection UV 260 nm
Sample 100 bp DNA Ladder (130 ng/µL)
Flow rate 0.3 mL/min Inj. Vol. 4.0 µL

pBR322/MspI digest

ap-1857.png

Conditions
Column COSMOSIL RNA-SEC-****
4.6 mm I.D. × 250 mm
Temperature 40℃
Mobile phase 20 mmol/L Phosphate Buffer (pH 7.0) Detection UV 260 nm
Sample pBR322/MspI digest (200 ng/µL)
Flow rate 0.3 mL/min Inj. Vol. 1.25 µL
その他の分析例はこちら

価格表

分析カラム(粒子径 5 µm)
COSMOSIL RNA-RP1

製品名サイズ製品番号価格
COSMOSIL RNA-RP1 Packed Column  2.0 mm l.D. × 100 mm 21078-31 e-Nacalai.jpg
4.6 mm l.D. × 100 mm 21079-21 e-Nacalai.jpg
10 mm l.D. × 100 mm 21080-81 e-Nacalai.jpg

COSMOSIL RNA-SEC-1000

製品名サイズ製品番号価格
COSMOSIL RNA-SEC-1000 Guard Column 7.5 mm l.D. × 50 mm 20785-91 e-Nacalai.jpg
COSMOSIL RNA-SEC-1000 Packed Column 4.6 mm l.D. × 250 mm 21088-01 e-Nacalai.jpg
7.5 mm l.D. × 300 mm 19380-21 e-Nacalai.jpg

COSMOSIL RNA-SEC-2000

製品名サイズ製品番号価格
COSMOSIL RNA-SEC-2000 Guard Column 7.5 mm l.D. × 50 mm 21096-91 e-Nacalai.jpg
COSMOSIL RNA-SEC-2000 Packed Column 4.6 mm l.D. × 250 mm 21095-01 e-Nacalai.jpg
7.5 mm l.D. × 300 mm 19381-11 e-Nacalai.jpg
※掲載内容は予告なく変更になる場合があります。