COSMOSIL Sugar-D NH2-MS|NACALAI TESQUE, INC.

COSMOSIL HPLC Columns

Saccharides are not retained on standard C18 columns because of the low hydrophobicity of compounds. COSMOSIL Sugar-D and NH2-MS are specifically designed for separation of saccharides. COSMOSIL C18-PAQ is recommended for hydrophobic glycosides or saccharide derivatives.

Specifications

Packing Material Sugar-D NH2-MS
Silica Gel High Purity Porous Spherical Silica
Average Particle Size 5 µm
Average Pore Size - approx. 120Å
Specific Surface Area - approx. 300m2/g
Stationary Phase - NH2-MS
Secondary/Tertiary Amine Aminopropyl
Bonding Type - Polymeric
Object Substance Monosaccharide, Oligosaccharide
End Capping Treatment - Near-perfect Treatment
Carbon Load - approx. 4%
Feature
  • High durability
  • Good quantitative analysis
  • Different selectivity from Sugar-D

COSMOSIL Sugar-D

Conventionally aminopropyl bonded stationary phases are used for liquid chromatographic analysis of mono- and oligosaccharides. General shortcomings of the conventional aminopropyl bonded phases are tailing and adsorption of certain saccharides and the general low durability (short active life) of these columns. These problems are addressed and solved by the novel COSMOSIL Sugar-D, resulting in better (sharper) separation and much improved durability.

Durability

Durability
Decomposition Condition
Solution Water
Flow Rate 1 ml/min
Temperature Room Temperature
Condition
Column Size 4.6 mm I.D. x 250 mm
Mobile Phase Acetonitrile : Water = 70 : 30
Flow Rate 1 ml/min
Temperature 30°C
Detection RI
Sample Maltose

Adsorption Characteristics

Certain types of saccharides such as arabinose or galactose are partially or temporarily adsorbed on conventional aminopropyl stationary phases causing tailing or no elution at all. COSMOSIL Sugar-D provides superior separation and high recovery for these saccharides.

absorption

Condition
Column Size 4.6 mm I.D. x 250 mm Sample Arabinose
Galactose
Maltitol
Mobile Phase Acetonitrile : Water = 70 : 30
Flow Rate 1 ml/min
Temperature 30°C
Detection RI

Applications

Mono- and Oligosaccharides
Mono- and Oligosaccharides
Condition
Column Size 4.6 mm I.D. x 250 mm
Mobile Phase Acetonitrile:Water =75:25
Flow Rate 1 ml/min
Temperature 30°C
Detection RI
Sample 1. Rhamnose 10 µg
2. Fructose 10 µg
3. Glucose 10 µg
4. Sucrose 10 µg
5. Maltose 10 µg
6. Raffinose 10 µg
Polyols
Polyols
Condition
Column Size 4.6 mm I.D. x 250 mm
Mobile Phase Acetonitrile:Water =75:25
Flow Rate 1 ml/min
Temperature 30°C
Detection RI
Sample 1. Glycerol 10 µg
2. meso-Erythritol 10 µg
3. Xylitol 10 µg
4. Glucitol 10 µg
5. Maltitol 10 µg
6. Inositol 10 µg
Oligomaltoses
Oligomaltoses
Condition
Column size 4.6 mm I.D. x 250 mm
Mobile Phase Acetonitrile:Water = 65:35
Flow Rate 1 ml/min
Temperature 30°C
Detection RI
Sample 1. Glucose 10 µg
2. Maltose 10 µg
3. Maltotriose 10 µg
4. Maltotetraose 10 µg
5. Maltopentaose 10 µg
6. Maltohexaose 10 µg
7. Maltoheptaose 10 µg

Comparison

The separation and the adsorption of monosaccharides were compared using COSMOSIL Sugar-D and other companies columns. Separation of aldoses, containing aldehyde group per molecule, is usually problematic with undesirable adsorption. COSMOSIL Sugar-D provides excellent separations for these saccharides.

comparison

Condition
Column size 4.6 mm I.D. x 250 mm Sample 1. Rhamnose 10 µg
Flow Rate 1 ml/min 2. Xylose 10 µg
Temperature 30°C 3. Fructose 10 µg
Detection RI 4. Mannose 10 µg
    5. Glucose 10 µg

comparison 8data

Retention

The conventional aminopropyl column is slightly more retentive than Sugar-D. The retention time of Sugar-D can be adjusted by increasing the concentration of acetonitrile in the mobile phase by 5%-10%.

Retention

Condition
Column size 4.6 mm I.D. x 250 mm Sample
  1. Glucose
  2. Maltose
Flow Rate 1 ml/min
Temperature 30°C
Detection RI

For more information on COSMOSIL 3.0 mm I.D. columns

Ordering Information

Analytical and Preparative Column (Particle size : 5 µm)
COSMOSIL Sugar-D
Product Name Size Product No.
COSMOSIL Sugar-D Guard Column
4.6 mm l.D. x 10 mm 05394-81
10 mm l.D. x 20 mm 05696-31
20 mm l.D. x 50 mm 05694-51
COSMOSIL Sugar-D Packed Column
2.0 mm l.D. x 250 mm 05689-31
3.0 mm l.D. x 150 mm 05690-91
3.0 mm l.D. x 250 mm 05691-81
4.6 mm l.D. x 150 mm 05395-71
4.6 mm l.D. x 250 mm 05397-51
10 mm l.D. x 250 mm 05692-71
20 mm l.D. x 250 mm 05693-61

NH2-MS

The COSMOSIL 5NH2-MS is a column with aminopropyl group bonded silica packing material. It is recommended for separation of monosaccharides and oligosaccharides. While Sugar-D is our first choice for separation of saccharide, 5NH2-MS can give better separation than Sugar-D in some cases depending on samples.

Comparison

comparison

Condition
Column Size 4.6 mm I.D . x 250 mm Sample 1. Sucrose 10 µg
Flow Rate 1 ml/min 2. Maltose 10 µg
Temperature 30°C 3. Lactose 10 µg
Detection RI 4. Trehalose 10 µg

Ordering Information

Analytical and Preparative Column (Particle size : 5 µm)
COSMOSIL NH2-MS
Product Name Size Product No.
COSMOSIL NH2-MS Guard Column
4.6 mm l.D x 10 mm 38241-51
10 mm l.D x 20 mm 38242-41
20 mm l.D x 50 mm 06093-91
COSMOSIL NH2-MS Packed Column
4.6 mm l.D x 150 mm 38245-11
4.6 mm l.D x 250 mm 38246-01
10 mm l.D x 250 mm 38249-71
20 mm l.D x 250 mm 38250-31

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