2本鎖DNAの定量を超感度かつ広範囲で行う。

 

 

 

 この製品に関するご意見・ご照会・お問合せはこちら

 

 AccuBlue™ /  AccuClear™

AccuAccuClear™ and AccuBlue™ dsDNA quantitation assays allow precise quantitation of purified dsDNA samples across a wide range of concentrations. Unlike absorbance-based nucleic acid quantitation, fluorescent DNA binding dyes are highly sensitive and selective for double-stranded DNA and provide a more accurate DNA concentration in the presence of contaminating RNA and other common contaminants including free nucleotides, protein, detergents and salts. Biotium offers three dsDNA quantitiation kits for different instruments and sample concentration ranges. The kits also differ in their tolerance of various contaminants; download the product protocols at www.biotium.com for details. The AccuClear™ Ultra High Sensitivity assay is based on a novel, next-generation DNA binding dye which offers unrivaled sensitivity and dynamic range compared to other DNA dyes.

 

AccuBlue™ Broad Range
• Linear range: 2-2000 ng dsDNA
• Blue fluorescence (Ex/Em: 350/460 nm)
• Assay can be extended to 4000 ng dsDNA with minor loss of linearity
• Blue fluorescence detection, compatible with fluorescence microplate reader

 

AccuBlue™ High Sensitivity
• Linear range: 0.2-100 ng dsDNA
• Green fluorescence (Ex/Em: 485/530 nm)
• Membrane-impermeable dye is non-toxic and non-mutagenic, for safer handling and easy disposal
• Green fluorescence detection compatible with fluorescence microplate reader, handheld fluorometer such as Qubit®, or NanoDrop® fluorescence reader

 

AccuClear Ultra High Sensitivity™
• Linear range: 0.03-250 ng dsDNA
• Green fluorescence (Ex/Em: 460/507 nm)
• Unrivaled sensitivity and dynamic range
• Novel green fluorescent dye is a perfect match for blue LED excitation sources
• Green fluorescence detection compatible with fluorescence microplate reader, handheld fluorometer such as Qubit®, or NanoDrop® fluorospectrometer

 

　　　　　　　　　　　　　        　　　　 

Figure 1: Triplicate samples of calf thymus dsDNA, mouse liver RNA or salmon sperm ssDNA were assayed with the AccuBlue High Sensitivity dsDNA Quantitation Kit. The AccuBlue High Sensitivity Assay is selective for double-stranded DNA over RNA or single-stranded DNA.

　　　　　　　　　　　　　　　　　          　　 

　　Figure 2 Quantitate a large number of dsDNA samples in four easy steps with AccuBlue™ or AccuClear™.

 

  

 

 

               AccuBlue™
          High Sensitivity Dye                                        A社                            

Figure 7: The AccuBlue High Sensitivity reagent and Company A were diluted to working concentrations in HeLa cell cultures and incubated for 30 minutes. Company A readily bind nuclear DNA while no nuclear staining is evident with the AccuBlue HS reagent, illustrating its overall safety and lower toxicity due to its membrane impermeability.

 

 

Ordering Information
Linear Range	Cat No.	Product Name	Kit Size
Broad Range 2-2000 ng	31009-T	AccuBlue Broad Range dsDNA Quantitation Solution, Trial Size	200 Assays
	31009	AccuBlue Broad Range dsDNA Quantitation Solution	1000 Assays
	31007	AccuBlue Broad Range dsDNA Quantitation Kit with 9 DNA standards	1000 Assays
High Sensitivity 0.2-100 ng	31008-T	AccuBlue High Sensitivity dsDNA Quantitation Solution, Trial Size	200 Assays
	31008	AccuBlue High Sensitivity dsDNA Quantitation Solution	1000 Assays
	31006	AccuBlue High Sensitivity dsDNA Quantitation Kit with 8 DNA Standards	1000 Assays
Ultra High Sensitivity 0.03-250 ng	31027-T	AccuClear Ultra High Sensitivity dsDNA Quantitation Solution, Trial Size	200 Assays
	31027	AccuClear Ultra High Sensitivity dsDNA Quantitation Solution	1000 Assays
	31028	AccuClear Ultra High Sensitivity dsDNA Quantitation Kit with 7 DNA Standards	1000 Assays
	31029	AccuClear Ultra High Sensitivity dsDNA Quantitation Kit with 1 DNA Standard	4000 Assays

                                                                                                                   

この製品に関するご意見・ご照会・お問合せはこちら