ナカライテスク

Wnt シグナル経路上のリン酸化タンパク質を特異的に検出

  

  

  

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Wnt Signaling Phospho Antibody Microarray

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 Introduction

The Wnt signal pathway involves a group of proteins that are responsible for cell interactions, proliferation, differentiation, migration and other functions in animal development. Studies have found that abnormal Wnt signaling is related to many human diseases including cancer and degenerative diseases.

The Wnt Signaling Phospho Antibody Array features 227 highly specific and well-characterized phosphorylation antibodies. It allows researchers to study the important proteins involved in this pathway. The phospho-specific antibodies bind to and detect the protein only when phosphorylated at the specific site.  The non-phospho pairs of the phospho-specific antibodies are included in the array to allow comparison based on phosphorylation state.  Each antibody is printed with six replicates, and the arrays are printed on standard-size coated glass microscope slides and can be scanned on all microarray scanners that are compatible with 76 x 25 x 1 mm (3 in. x 1 in. x 1mm) slides.       

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Key Features

• Unique collection of phospho-specific antibodies and their non-phospho pairs

• Microarray format for high throughput expression profiling protein extracts from cells, frozen or FFPE tissues, or serum samples

• Each antibody is spotted with six replicates for more reliable and consistent results

• 3-D polymer based glass substrates

• High specificity and affinity; low background

• Suitable for detection on all fluorescent microarray scanners compatible with 76x25x1mm slides

• GenePix^® Array List (GAL) files are provided for convenient and accurate data analysis

• Antibody Array Assay Kit provides optimized reagents and buffers for protein extraction, labeling, conjugation and detection

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Applications

• Protein expression profiling (Qualitative)

• Measure changes in phosphorylation status at specific sites

• Compare profiles of normal, diseased or treated samples

• Identify candidate biomarkers

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Specifications

Slide dimensions:	76 x 25 x 1 mm	Spot diameter:	260 um
Barcode label:	23 x 9 mm	Spot-to-spot distance:	600 um

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Technical Information

Antibody Array User's Guide

Antibody List / Reactivity

Array Layout

GAL File

Frequently Asked Questions

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Components

Antibody arrays (2)

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Storage Conditions

4°C for 6 months

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Ordering Information

Cat. No.	Description	Reactivity	No. of Antibodies	Size
PNT227	Wnt Signaling Phospho Antibody Array	Human Mouse	227	2 slides

Related Products and Services

Cat. No.	Description	Size
KAS02	Antibody Array Assay Kit, 2 Reactions	2 Reactions
KAS20	Antibody Array Assay Kit, 20 Reactions	20 Reactions
SEV03	Complete Antibody Array Assay Service, Wnt Signaling Phospho Array (PNT227)	1 sample

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