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VeriKine-HS^TM Human Interferon Beta Serum ELISA Kit

 

• Allows accurate Human IFN-β measurement to as low as 1.2 pg/ml

• Compatibles with human serum, plasma, autoimmune disease sera, or cell culture media

• Provides high precision assay reproducibility with < 8% inter-assay CV

• Suitable for measurement of trademarked therapeutic molecules in human serum samples

 

 

 

Interferon beta (IFN-β) has long been known to function as an inhibitor of viral
replication as part of body’s innate antiviral response. It is also widely used as a
treatment for Multiple Sclerosis (MS) patients, either as a single agent or as part of a
combinatorial approach. With the resurgence of interest in antitumor immunity as a
promising treatment approach for several types of cancers, there has been renewed
focus on applying IFN-β as a therapy due to the anti-tumor and immunomodulatory
properties of the protein. In addition, ligation of Toll-Like Receptors (TLRs)
leads to IFN-β production and, as novel TLR agonists and antagonists are being
developed as therapeutics for several diseases, it may be useful to monitor IFN-β
level as a downstream readout for compound effectiveness.

The VeriKine-HS^TM Human Interferon Beta Serum ELISA kit has been developed
to measure low or basal levels of human IFN-β in serum, plasma or tissue culture
media samples. This assay is also suitable for measurement of trademarked IFN-β
therapeutic compounds in human serum. Researchers and clinical investigators
examining a) the pharmacokinetics of IFN-β molecules, b) IFN-β as a biomarker,
or c) IFN-β as a pharmacodynamic marker of TLR agent or other immune response modifier activity will find this highly sensitive and specific assay to be an essential laboratory tool.

 

 

 

 

Dose Response in Normal Human Serum Spiked with 0, 1, 3, and 10 U/ml IFN-β

 

 

 

Correlation between ELISA Standard Curves prepared in Standard Diluent and Normal Human Serum

 

  

 

 

 

                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            

Catalog Number	Description	Package Size
41415-1	VeriKine-HSTM Human Interferon Beta Serum ELISA Kit Compatibility: human serum, plasma, cell culture media plate Assay range: 1.2-150 pg/ml	1 x 96-stripwell

 

Kit Speci!cations:
• Assay range: 1.2-150 pg/ml
• Format: 96-stripwell plate
• Sample volume: 50 μl per well
• Detection: colorimetric
• Assay time: 3 hours
• Compatibility: human serum, plasma,　or cell culture media
• Speci!city: human IFN-β. Does not　cross-react with human IFN-α,
IFN-γ, IFN-ω, IL-6; no cross-reactivity with　mouse IFN-α, IFN-β or Rat IFN-β

 

Kit Components:
• Precoated 96-stripwell plate
• Plate sealer
• Recombinant human IFN-β standard
• Wash solution concentration
• Standard diluent
• Sample buller
• Antibody concentrate
• HRP conjugate concentrate
• Assay diluent
• TMB substract solution
• Stop solution

 

 

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