NACALAI TESQUE, INC.

COSMOSIL alkyl type columns also include stationary phases with packing materials of C₂₂ (Dococyl group), C₈ (Octadecyl group), C₄(Butyl group) and TMS (Trimethyl group). The order of retention force by hydrophobicity of each packing material is C₂₂=C₁₈>C₈>C₄>TMS. The columns having lower hydrophobicity than C₁₈ or C₂₂ are effective for separation of high hydrophobic compounds and compounds with big difference in hydrophobic. Hydrophobicity of C₂₂ is about the same as C₁₈. However, stereoselectivity of C₂₂ is higher than C₁₈, and so C₂₂ may provide better separation.

Material characteristics

Packing material	C22-AR-II	C18-MS-II	C8-MS	C4-MS	TMS-MS
Silica gel	high purity porous spherical silica
Average particle size	5 m
Average pore size	approx. 120
Specific surface area	approx. 300 m2/g
Stationary phase	dococyl group	octadecyl group	octyl group	butyl group	trimethyl group
Bonding type	polymeric	monomeric
Main interaction	hydrophobic interaction
End capping treatment	near-perfect treatment
Carbon content	approx. 19%	approx. 16%	approx. 10%	approx. 7%	approx. 5%

Effect of alkyl chain length on reversed phase

The shorter alkyl chain stationary phase shows shorter retention time for non-polar compounds such as benzene and toluene and longer retention for polar compounds such as acetophenone and benzoate.

Condition
Column size	4.6 mm I.D. x 150 mm	Sample	1. Acetophenone	0.05 g
Mobile phase	methanol : water = 60 : 40		2. Methyl Benzoate	0.5 g
Flow rate	1.0 ml/min		3. Benzene	2.0 g
Temperature	30°C		4. Toluene	2.0 g
Detection	UV 254 nm

Ordering information

Analytical and preparative column (Particle size : 5 μm)

COSMOSIL 5C₂₂-AR-II

Product name	Size	Product No.
COSMOSIL 5C22-AR-II Guard Column
	4.6 mm I.D. x 10 mm	04881-21
	10 mm I.D. x 20 mm	05554-81
COSMOSIL 5C22-AR-II Packed Column
	4.6 mm I.D. x 50 mm	05848-41
	4.6 mm I.D. x 100 mm	05849-31
	4.6 mm I.D. x 150 mm	04598-51
	4.6 mm I.D. x 250 mm	04599-41
	6.0 mm I.D. x 150 mm	05850-91
	6.0 mm I.D. x 250 mm	05851-81
	10 mm I.D. x 250 mm	04969-91
	20 mm I.D. x 250 mm	05183-41

COSMOSIL 5C₈-MS

Product name	Size	Product No.
COSMOSIL 5C8-MS Guard Column
	4.6 mm I.D. x 10 mm	38151-31
	10 mm I.D. x 20 mm	38152-21
COSMOSIL 5C8-MS Packed Column
	4.6 mm I.D. x 50 mm	38153-11
	4.6 mm I.D. x 100 mm	38154-01
	4.6 mm I.D. x 150 mm	38155-91
	4.6 mm I.D. x 250 mm	38156-81
	6.0 mm I.D. x 150 mm	38157-71
	6.0 mm I.D. x 250 mm	38158-61
	10 mm I.D. x 250 mm	38159-51
	20 mm I.D. x 250 mm	38160-11

COSMOSIL 5C₄-MS

Product name	Size	Product No.
COSMOSIL 5C4-MS Guard Column
	4.6 mm I.D. x 10 mm	38161-01
	10 mm I.D. x 20 mm	38162-91
COSMOSIL 5C4-MS Packed Column
	4.6 mm I.D. x 50 mm	38163-81
	4.6 mm I.D. x 100 mm	38164-71
	4.6 mm I.D. x 150 mm	38165-61
	4.6 mm I.D. x 250 mm	38166-51
	6.0 mm I.D. x 150 mm	38167-41
	6.0 mm I.D. x 250 mm	38168-31
	10 mm I.D. x 250 mm	38169-21
	20 mm I.D. x 250 mm	38170-81

COSMOSIL 5TMS-MS

Product name	Size	Product No.
COSMOSIL 5TMS-MS Guard Column
	4.6 mm I.D. x 10 mm	38171-71
	10 mm I.D. x 20 mm	38172-61
COSMOSIL 5TMS-MS Packed Column
	4.6 mm I.D. x 50 mm	38173-51
	4.6 mm I.D. x 100 mm	38174-41
	4.6 mm I.D. x 150 mm	38175-31
	4.6 mm I.D. x 250 mm	38176-21
	6.0 mm I.D. x 150 mm	38177-11
	6.0 mm I.D. x 250 mm	38178-01
	10 mm I.D. x 250 mm	38179-91
	20 mm I.D. x 250 mm	38180-51