Products

COSMOSIL Protein-R

Features

Column Image

  • Excellent separation
  • High recovery rate
  • Outstanding stability at low pH

Product Information

Specifications

Packing Material Protein-R
USP Code L1
Silica Gel High Purity Porous Spherical Silica
Average Particle Size 5 µm
Average Pore Size approx. 300Å
Specific Surface Area approx. 150 m2/g
Stationary Phase Octadecyl Group
Bonding Type Polymeric
Main Interactions Hydrophobic Interaction
End Capping Treatment Near-perfect Treatment
pH Range 1.5-7.5*
Feature - High recovery rate
- Acid-resistance

* Optimal pH range of silica-based columns is between 2 and 7.5. Extreme pH may significantly decrease column lifetime.

Comparison of Separation

Protein-R shows sharper peaks for proteins than conventional C18 wide pore columns.

Protein-R

Comparison of separation

5C18-300

Comparison of separation

Condition
Column Size 4.6 mm I.D. x 150 mm Sample
  1. Ribonuclease A 1.0 µg
  2. Cytochrome C 1.0 µg
  3. Lysozyme 1.0 µg
  4. BSA 1.0 µg
  5. Myoglobin 1.0 µg
  6. Ovalbumin 1.0 µg
Mobile Phase A : 0.05%TFA - 20% Acetonitrile
B : 0.05%TFA - 60% Acetonitrile
B conc. 0 → 35% 60 min Linear Gradient
Flow Rate 1.0 ml/min
Temperature 30°C
Detection UV 220 nm

Recovery Rate

The figure below shows recovery rates for proteins using different columns. Protein-R shows a higher recovery rate than C4-300 and a much higher recovery rate than C18-300.

recoveryrate

Durability

The figure below shows durability against acidic mobile phase of various columns. Protein-R shows a higher acid durability than C4-300.

durability

Protein Separation Wide Pore Columns

Reversed Phase ChromatographyProtein-R
C18-AR-300・C8-AR-300・C4-AR-300・Ph-AR-300
Gel Filtration ChromatographyDiol-120-II ・Diol-300-II
Hydrophobic Interaction ChromatographyHIC

Applications

Column5 µm Protein-R
I.D. (mm)2.04.610.020.0
Washing method Washing method
1. Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid.
2. (If you used a salt/acid, always do step 1 first!) Remove adsorbed compounds and fix unstable baselines by washing with 50-70% acetonitrile in water containing 0.1% trifluoroacetic acid.
Caution: Some proteins may precipitate when concentration of organic solvent is high.
Storage conditions Short-term (up to a week):
Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. For example, if your mobile phase was 50:50 methanol/20 mmol/l phosphate buffer, wash with 50:50 methanol/water.

Long-term:
Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. Then, replace the solvent with 70% methanol or acetonitrile : 30% water.

In either case, tightly plug the column, and store in a cool and dry place.
Usable pH range 1.5-7.5 (pH 2-7.5 recommended)
Max. pressure 20MPa 15MPa
Temperature range The maximum usable temperature is 60°C. However. for regular use, please use at a constant temperature between 20°C and 50°C.
Usable solvents Any solvent that will not dissolve the silica gel (such as alkaline solutions) or cleave the stationary phase (such as very acidic solutions) is usable.
Note: For solvents with high viscosity, please watch the system pressure and keep it below 20 MPa. Also, please wash the column after using acidic mobile phases or solvents with high freezing points.

Usage information

Column5 µm Protein-R
I.D. (mm)2.04.610.020.0
Washing method Washing method
1. Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid.
2. (If you used a salt/acid, always do step 1 first!) Remove adsorbed compounds and fix unstable baselines by washing with 50-70% acetonitrile in water containing 0.1% trifluoroacetic acid.
Caution: Some proteins may precipitate when concentration of organic solvent is high.
Storage conditions Short-term (up to a week):
Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. For example, if your mobile phase was 50:50 methanol/20 mmol/l phosphate buffer, wash with 50:50 methanol/water.

Long-term:
Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. Then, replace the solvent with 70% methanol or acetonitrile : 30% water.

In either case, tightly plug the column, and store in a cool and dry place.
Usable pH range 1.5-7.5 (pH 2-7.5 recommended)
Max. pressure 20MPa 15MPa
Temperature range The maximum usable temperature is 60°C. However. for regular use, please use at a constant temperature between 20°C and 50°C.
Usable solvents Any solvent that will not dissolve the silica gel (such as alkaline solutions) or cleave the stationary phase (such as very acidic solutions) is usable.
Note: For solvents with high viscosity, please watch the system pressure and keep it below 20 MPa. Also, please wash the column after using acidic mobile phases or solvents with high freezing points.

Downloads

Brochures

  • COSMOSIL Protein-R / Wide Pore Columns pdf (2.4 MB)

Ordering Information

COSMOSIL Protein-R

Product Name Size Product No. Price
COSMOSIL Protein-R Guard Column
4.6 mm l.D. x 10 mm 06518-31 e-Nacalai
10 mm l.D. x 20 mm 06528-01
COSMOSIL Protein-R Packed Column
2.0 mm l.D. x 150 mm 06514-71 e-Nacalai
4.6 mm l.D. x 50 mm 06525-31
4.6 mm l.D. x 150 mm 06526-21
4.6 mm l.D. x 250 mm 06527-11
10 mm l.D. x 150 mm 06529-91
10 mm l.D. x 250 mm 06530-51
20 mm l.D. x 150 mm 06531-41
20 mm l.D. x 250 mm 06532-31

(Storage) RT: Room temperature, A: Cool and dark, R: Refrigerator, F: Freeze