COSMOSIL HPLC Columns
COSMOSIL® Protein-R
COSMOSIL Protein-R is a reversed phased HPLC column designed specifically for peptide and protein separations. This column provides improved separation, high recovery rate and acid-resistant, which are often problematic to separate using conventional C18-300 (wide pore) and C4-300 (wide pore).
Material characteristics
| Packing material | Protein-R |
|---|---|
| Silica gel | high purity porous spherical silica |
| Average particle size | 5 m |
| Average pore size | approx. 300 |
| Specific surface area | approx. 150 m2/g |
| Stationary phase | octadecyl group |
| Bonding type | polymeric |
| Main interactions | hydrophobic interaction |
| End capping treatment | near-perfect treatment |
| Feature | - High recovery rate - Acid-resistance |
Comparison of separation
Protein-R shows sharper peaks for proteins than conventional C18 wide pore columns.
Protein-R |
5C18-300 |
|---|---|
 |
 |
| Condition | |||
|---|---|---|---|
| Column size | 4.6 mm I.D. x 150 mm | Sample |
|
| Mobile phase | A : 0.05%TFA - 20% acetonitrile | ||
| B : 0.05%TFA - 60% acetonitrile | |||
| B conc. 0 → 35% 60 min linear gradient | |||
| Flow rate | 1.0 ml/min | ||
| Temperature | 30°C | ||
| Detection | UV 220 nm | ||
Recovery rate
The figure below shows recovery rates for proteins using different columns. Protein-R shows a higher recovery rate than C4-300 and a much higher recovery rate than C18-300.
Durability
The figure below shows durability against acidic mobile phase of various columns. Protein-R shows a higher acid durability than C4-300.
Application of peptide separation
5C18-MS-II (pore size 120) shows better separation of low molecular weight proteins, but Protein-R shows better separation of high-molecular weight proteins.
| Condition | |||
|---|---|---|---|
| Column size | 4.6 mm I.D. x 150 mm | Temperature | 30°C |
| Mobile phase | A : 0.05%TFA - water | Detection | UV 220 nm |
| B : 0.05%TFA - acetonitrile | Sample | Poly-L-Glutamic Acid Sodium Salt | |
| B conc. 0 → 35% /60min linear gradient | (Molecular weight:1500-5000) | ||
| Flow rate | 1 ml/min | (Concentration: 2.0 mg/ml） | |
Ordering information
COSMOSIL Protein-R
| Product name | Size | Product No. |
|---|---|---|
| COSMOSIL Protein-R Guard Column | ||
| 4.6 mm l.D. x 10 mm | 06518-31 | |
| 10 mm l.D. x 20 mm | 06528-01 | |
| COSMOSIL Protein-R Packed Column | ||
| 2.0 mm l.D. x 150 mm | 06514-71 | |
| 4.6 mm l.D. x 50 mm | 06525-31 | |
| 4.6 mm l.D. x 150 mm | 06526-21 | |
| 4.6 mm l.D. x 250 mm | 06527-11 | |
| 10 mm l.D. x 150 mm | 06529-91 | |
| 10 mm l.D. x 250 mm | 06530-51 | |
| 20 mm l.D. x 150 mm | 06531-41 | |
| 20 mm l.D. x 250 mm | 06532-31 |
Protein Separation Wide Pore Columns
- Reversed phase chromatography
- Protein-R
C18-AR-300･C8-AR-300･C4-AR-300･Ph-AR-300 - Gel filtration chromatography
- Diol-120-II ･Diol-300-II
- Hydrophobic interaction chromatography
- HIC
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