高輝度・長寿命の次世代蛍光色素Biotium社 CF™ Dye
CF™ Dye は、Biotium 社より開発された次世代の蛍光色素です。本蛍光色素は、高輝度でかつ退色耐久性に優れた性質を持っています。また、従来の蛍光色素よりも低電荷であり、標識体の性質に影響の少ない蛍光色素です。本色素の標識二次抗体をはじめとするさまざまな標識体、反応性誘導体、ラベリングキットなどを紹介します。
特長
- 高輝度・高い退色耐久性
- 標識体の性質に影響を与えにくい低電荷の蛍光色素
- 標識二次抗体をはじめ、各種標識体、反応性誘導体やラベリングキットを用意
使用例
CF™ 488A & CF™ 555 標識二次抗体を用いたプラナリア脳の神経軸索の染色
左：CF™ 488A および CF™ 555 標識二次抗体を用いた染色
右：A 社標識二次抗体を用いた染色
サンプル
プラナリア脳（ホールマウント、観察時 200 µm の厚さに圧縮）
固定法 カルノア法（120 min, 4℃）
透過処理 キシレン：エタノール=1:1（60 min, 室温）
脳のドーパミン神経の染色
一次抗体
Rabbit Anti-Planarian TH
二次抗体
Biotium 社 CF™ 555 Goat Anti-Rabbit IgG（H+L）
A 社 橙色蛍光標識 Goat Anti-Rabbit IgG（H+L）
脳のGABA神経の染色
一次抗体
Mouse Anti-Planarian GAD
二次抗体
Biotium 社 CF™ 488A Goat Anti-Mouse IgG（H+L）
A 社 緑色蛍光標識 Goat Anti-Mouse IgG（H+L）
核の染色
Hoechst 33342
結果
Olympus FV10 を使用して、A 社製品と同条件で検出した結果、CF™ Dye は、A 社製品と同程度もしくはそれ以上の感度で検出でき、表面から 15 µm という深度の深い場所でも、神経軸索の一本一本まで検出が可能でした。
データご提供
京都大学 大学院理学研究科 生物科学専攻 高次情報形成学講座 生体情報発生分野 分子発生学分科
阿形 清和 教授 / 井上 武 助教
製品概要
CF™ Dye には、UV から近赤外領域までのさまざまな蛍光色素が用意されています。標識二次抗体以外にもさまざまな CF™ Dye 標識体、反応性誘導体、ラベリングキットも用意しています。
CF™ Dye 各蛍光色素を用いた製品ラインアップ
ラベリングキットと関連製品 EverBrite™ Mounting Medium の詳細はこちら。
製品使用文献
製品使用文献はこちらのページで閲覧できます。
https://www.nacalai.co.jp/information/literature/BiotiumCFDye.cfm
CF™ 350
製品説明
CF™ 350 標識二次抗体の励起/蛍光スペクトル
Technical Summary
極大励起波長 / 極大蛍光波長：347 / 448 nm
分子吸光係数：18,000
分子量：〜496
フローサイトメトリーレーザーライン：UV
顕微鏡レーザーライン：UV
CF™ 350 標識二次抗体の蛍光強度の比較
CF™ 350 と A 社蛍光色素標識の Goat Anti-Mouse IgG を用いて、相対的な蛍光強度を測定しました。CF™ 350 は A 社製品と同等以上の結果が得られました。
DOL：蛍光標識の程度
CF™ 350 標識二次抗体を用いた染色
HeLa 細胞を、一次抗体に抗 α-チューブリン抗体（マウス）、二次抗体に CF™ 350 標識 Goat Anti-Mouse IgG を用いて染色しました。
価格表
| 製品名 | メーカー 製品番号 | 容量 | オンライン カタログへ |
|---|---|---|---|
| CF™350 Donkey Anti-Goat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20142-1 | 50 µL |  |
| CF™350 Donkey Anti-Goat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20142 | 0.5 mL | |
| CF™350 Goat Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20143-1 | 50 µL | |
| CF™350 Goat Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20143 | 0.5 mL | |
| CF™350 Goat Anti-Rabbit IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20144-1 | 50 µL | |
| CF™350 Goat Anti-Rabbit IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20144 | 0.5 mL | |
| CF™350 Goat Anti-Rat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20147-1 | 50 µL | |
| CF™350 Goat Anti-Rat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20147 | 0.5 mL | |
| CF™350 Donkey Anti-sheep IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20148-1 | 50 µL | |
| CF™350 Donkey Anti-sheep IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20148 | 0.5 mL | |
| CF™350 Rabbit Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20149-1 | 50 µL | |
| CF™350 Rabbit Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20149 | 0.5 mL | |
| CF™350 Donkey Anti-Chicken IgY(IgG)(H+L), Highly Cross-adsorbed, 2 mg/mL | 20275-1 | 50 µL | |
| CF™350 Donkey Anti-Chicken IgY(IgG)(H+L), Highly Cross-adsorbed, 2 mg/mL | 20275 | 0.5 mL | |
| CF™350 Donkey Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20350-1 | 50 µL | |
| CF™350 Donkey Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20350 | 0.5 mL | |
| CF™350 Donkey Anti-Rabbit IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20351-1 | 50 µL | |
| CF™350 Donkey Anti-Rabbit IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20351 | 0.5 mL | |
| CF™350 Donkey Anti-Rat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20361-50uL | 50 µL | |
| CF™350 Donkey Anti-Rat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20361-500uL | 0.5 mL | |
| CF™350 Goat Anti-Mouse IgG(H+L), 2 mg/mL | 20140-1 | 50 µL | |
| CF™350 Goat Anti-Mouse IgG(H+L), 2 mg/mL | 20140 | 0.5 mL | |
| CF™350 Goat Anti-Rabbit IgG(H+L), 2 mg/mL | 20141-1 | 50 µL | |
| CF™350 Goat Anti-Rabbit IgG(H+L), 2 mg/mL | 20141 | 0.5 mL | |
| CF™350 Goat Anti-Guinea Pig IgG(H+L), 2 mg/mL | 20198-1 | 50 µL | |
| CF™350 Goat Anti-Guinea Pig IgG(H+L), 2 mg/mL | 20198 | 0.5 mL | |
| CF™350 Goat Anti-Mouse IgG1(γ1), 2 mg/mL | 20245-1 | 50 µL | |
| CF™350 Goat Anti-Mouse IgG1(γ1), 2 mg/mL | 20245 | 0.25 mL | |
| CF™350 Goat Anti-Mouse IgG2a(γ2a), 2 mg/mL | 20255-1 | 50 µL | |
| CF™350 Goat Anti-Mouse IgG2a(γ2a), 2 mg/mL | 20255 | 0.25 mL | |
| CF™350 Goat Anti-Mouse IgG2b(γ2b), 2 mg/mL | 20265-1 | 50 µL | |
| CF™350 Goat Anti-Mouse IgG2b(γ2b), 2 mg/mL | 20265 | 0.25 mL | |
| CF™350 Chicken Anti-Mouse IgG(H+L), 2 mg/mL | 20331-50uL | 50 µL | |
| CF™350 Chicken Anti-Mouse IgG(H+L), 2 mg/mL | 20331-500uL | 0.5 mL | |
| CF™350 Chicken Anti-Rabbit IgG(H+L), 2 mg/mL | 20332-50uL | 50 µL | |
| CF™350 Chicken Anti-Rabbit IgG(H+L), 2 mg/mL | 20332-500uL | 0.5 mL | |
| CF™350 Chicken Anti-Goat IgG(H+L), 2 mg/mL | 20364-50uL | 50 µL | |
| CF™350 Chicken Anti-Goat IgG(H+L), 2 mg/mL | 20364-500uL | 0.5 mL | |
| CF™350 F(ab')2 Fragment of Goat Anti-Mouse IgG(H+L), 2 mg/mL | 20145 | 0.25 mL | |
| CF™350 F(ab')2 Fragment of Goat Anti-Rabbit IgG(H+L), 2 mg/mL | 20146 | 0.25 mL | |
| Annexin V, CF™350 Conjugate | 29012 | 0.5 mL | |
| Wheat Germ Agglutinin, CF™350 Conjugate | 29021-1 | 1 mg | |
| Wheat Germ Agglutinin, CF™350 Conjugate | 29021 | 5×1 mg | |
| Concanavalin A, CF™350 Conjugate | 29015 | 5 mg | |
| Streptavidin, CF™350 Conjugate | 29031 | 1 mg | |
| Phalloidin, CF™350 | 00049-T | 50 U | |
| Phalloidin, CF™350 | 00049 | 300 U | |
| CF™350, Aminooxy | 92050 | 1 mg | |
| CF™350 Amine | 92035 | 1 mg | |
| CF™350 Succinimidyl Ester | 92109 | 1 µmol | |
| CF™350 Hydrazide | 92151 | 1 mg | |
| CF™350 Maleimide | 92020 | 1 µmol | |
| Mix-n-Stain™ CF™350 Antibody Labeling Kit, 1×(5-20 µg) labeling | 92270 | 1 kit | |
| Mix-n-Stain™ CF™350 Antibody Labeling Kit, 1×(20-50 µg) labeling | 92250 | 1 kit | |
| Mix-n-Stain™ CF™350 Antibody Labeling Kit, 1×(50-100 µg) labeling | 92230 | 1 kit | |
| CF™350 Protein Labeling Kit 3 labelings | 92210 | 1 kit | |
CF™ 405S
使用例
CF™ Dye 標識体による多重染色
HeLa 細胞を、一次抗体に抗 golgin97 抗体（マウス）および抗 COX IV 抗体（ラビット）を用い、それぞれの二次抗体に CF™ 405S 標識の Goat Anti-Mouse IgG、CF™ 488A 標識の Goat Anti-Rabbit IgG を用いて染色しました。F-アクチンは CF™ 555 ファロイジンを用いて染色しました。
青：ゴルジ体、緑：ミトコンドリア、赤：細胞骨格
価格表
| 製品名 | メーカー 製品番号 | 容量 | オンライン カタログへ |
|---|---|---|---|
| CF™405S Donkey Anti-Goat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20416-50uL | 50 µL | |
| CF™405S Donkey Anti-Goat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20416-500uL | 500 µL | |
| CF™405S Donkey Anti-Guinea Pig IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20356-50uL | 50 µL | |
| CF™405S Donkey Anti-Guinea Pig IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20356-500uL | 0.5 mL | |
| CF™405S Goat Anti-Mouse IgG(H+L), 2 mg/mL | 20080-1 | 50 µL | |
| CF™405S Goat Anti-Mouse IgG(H+L), 2 mg/mL | 20080 | 0.5 mL | |
| CF™405S Goat Anti-Rabbit IgG(H+L), 2 mg/mL | 20082-1 | 50 µL | |
| CF™405S Goat Anti-Rabbit IgG(H+L), 2 mg/mL | 20082 | 0.5 mL | |
| CF™405S Goat Anti-Mouse IgG1(γ1), 2 mg/mL | 20380-50uL | 50 µL | |
| CF™405S Goat Anti-Mouse IgG1(γ1), 2 mg/mL | 20380-250uL | 0.25 mL | |
| CF™405S Goat Anti-Mouse IgG2a(γ2a), 2 mg/mL | 20381-50uL | 50 µL | |
| CF™405S Goat Anti-Mouse IgG2a(γ2a), 2 mg/mL | 20381-250uL | 0.25 mL | |
| CF™405S Goat Anti-Mouse IgG2b(γ2b), 2 mg/mL | 20382-50uL | 50 µL | |
| CF™405S Goat Anti-Mouse IgG2b(γ2b), 2 mg/mL | 20382-250uL | 0.25 mL | |
| CF™405S Monoclonal Mouse Anti-Biotin IgG, 2 mg/mL | 20203-1 | 50 µL | |
| CF™405S Monoclonal Mouse Anti-Biotin IgG, 2 mg/mL | 20203 | 0.25 mL | |
| Wheat Germ Agglutinin, CF™405S Conjugate | 29027-1 | 1 mg | |
| Wheat Germ Agglutinin, CF™405S Conjugate | 29027 | 5×1 mg | |
| Streptavidin, CF™405S Conjugate | 29032 | 1 mg | |
| α-Bungarotoxin, CF™405S | 00002 | 500 µg | |
| CF™405S-dUTP, lyophilized powder | 40004 | 25 nmol | |
| CF™405S, Aminooxy | 92055 | 1 mg | |
| CF™405S Amine | 92036 | 1 mg | |
| CF™405S Succinimidyl Ester | 92110 | 1 µmol | |
| CF™405S Maleimide | 92030 | 1 µmol | |
| Mix-n-Stain™ CF™405S Antibody Labeling Kit, 1×(5-20 µg) labeling | 92271 | 1 kit | |
| Mix-n-Stain™ CF™405S Antibody Labeling Kit, 1×(20-50 µg) labeling | 92251 | 1 kit | |
| Mix-n-Stain™ CF™405S Antibody Labeling Kit, 1×(50-100 µg) labeling | 92231 | 1 kit | |
| CF™405S Protein Labeling Kit 3 labelings | 92211 | 1 kit | |
CF™ 405M
製品説明
CF™ 405M 標識二次抗体の励起/蛍光スペクトル
Technical Summary
極大励起波長 / 極大蛍光波長：408 / 452 nm
分子吸光係数：41,000
分子量：～503
フローサイトメトリーレーザーライン：405 nm
顕微鏡レーザーライン：405 nm
CF™ 405M 標識二次抗体の蛍光強度の比較
Jurkat 細胞を、一次抗体に intracellular CD3 に対する抗体、二次抗体に CF™ 405M または A 社の蛍光色素標識の Goat Anti-Mouse IgG を用い、BD™ LSR II フローサイトメーター（405 バイオレットレーザーと 450/50 nm 検出器搭載）により解析しました。グラフは細胞集団の蛍光強度の相乗平均を表しています。バックグラウンドは二次抗体のみを用いて測定しました。
CF™ 405M 色素の退色性の比較
同じ濃度の CF™ 405M 色素溶液と A 社色素溶液を、水銀ランプを光源とする蛍光顕微鏡上で暴露し、DAPI フィルターセットを用いて蛍光強度を測定しました。5 秒間隔で蛍光強度を測定し、0 秒の値により標準化しました。
価格表
| 製品名 | メーカー 製品番号 | 容量 | オンライン カタログへ |
|---|---|---|---|
| CF™405M Goat Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20182-1 | 50 µL | |
| CF™405M Goat Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20182 | 0.5 mL | |
| CF™405M Goat Anti-Mouse IgG(H+L), Highly Cross-adsorbed(min X Rat), 2 mg/mL | 20340-50uL | 50 µL | |
| CF™405M Goat Anti-Mouse IgG(H+L), Highly Cross-adsorbed(min X Rat), 2 mg/mL | 20340-500uL | 0.5 mL | |
| CF™405M Goat Anti-Rabbit IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20373-50uL | 50 µL | |
| CF™405M Goat Anti-Rabbit IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20373-500uL | 0.5 mL | |
| CF™405M Goat Anti-Rat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20374-50uL | 50 µL | |
| CF™405M Goat Anti-Rat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20374-500uL | 0.5 mL | |
| CF™405M Goat Anti-Chicken IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20375-50uL | 50 µL | |
| CF™405M Goat Anti-Chicken IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20375-500uL | 0.5 mL | |
| CF™405M Donkey Anti-Guinea Pig IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20376-50uL | 50 µL | |
| CF™405M Donkey Anti-Guinea Pig IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20376-500uL | 0.5 mL | |
| CF™405M Goat Anti-Mouse IgG(H+L), 2 mg/mL | 20180-1 | 50 µL | |
| CF™405M Goat Anti-Mouse IgG(H+L), 2 mg/mL | 20180 | 0.5 mL | |
| CF™405M Goat Anti-Rabbit IgG(H+L), 2 mg/mL | 20181-1 | 50 µL | |
| CF™405M Goat Anti-Rabbit IgG(H+L), 2 mg/mL | 20181 | 0.5 mL | |
| CF™405M Monoclonal Mouse Anti-Fluorescein(FITC) IgG, 2 mg/mL | 20214-1 | 50 µL | |
| CF™405M Monoclonal Mouse Anti-Fluorescein(FITC) IgG, 2 mg/mL | 20214 | 0.25 mL | |
| Annexin V, CF™405M Conjugate | 29009 | 0.5 mL | |
| Wheat Germ Agglutinin, CF™405M Conjugate | 29028-1 | 1 mg | |
| Wheat Germ Agglutinin, CF™405M Conjugate | 29028 | 5×1 mg | |
| Streptavidin, CF™405M Conjugate | 29033 | 1 mg | |
| Phalloidin, CF™405M | 00034-T | 50 U | |
| Phalloidin, CF™405M | 00034 | 300 U | |
| CF™405M Azide | 92092 | 0.5 mg | |
| CF™405M Aminooxy | 92056 | 1 mg | |
| CF™405M Alkyne | 92093 | 0.5 mg | |
| CF™405M Succinimidyl Ester | 92111 | 1 µmol | |
| CF™405M Maleimide | 92021 | 1 µmol | |
| Mix-n-Stain™ CF™405M Antibody Labeling Kit, 1×(5-20 µg) labeling | 92272 | 1 kit | |
| Mix-n-Stain™ CF™405M Antibody Labeling Kit, 1×(20-50 µg) labeling | 92252 | 1 kit | |
| Mix-n-Stain™ CF™405M Antibody Labeling Kit, 1×(50-100 µg) labeling | 92232 | 1 kit | |
| CF™405M Protein Labeling Kit 3 labelings | 92212 | 1 kit | |
CF™ 488A
製品説明
CF™ 488A 標識二次抗体の励起/蛍光スペクトル
Technical Summary
極大励起波長 / 極大蛍光波長：490 / 515 nm
分子吸光係数：70,000
分子量：〜910
フローサイトメトリーレーザーライン：488 nm
顕微鏡レーザーライン：488 nm
CF™ 488A 標識二次抗体の蛍光強度の比較
ヒト心臓の凍結組織切片（6µm）を、一次抗体に抗フィブロネクチン抗体、二次抗体に各社蛍光色素標識の Goat Anti-Rabbit IgG を用いて染色しました。その結果、CF™ 488A 標識二次抗体は、他社製品と同様に使用できることが示されています。
データ提供：Dr. Sawa Kostin at Max-Planck-Institute für Herz- und Lungenforschung （W.G. Kerckhoff-Institut） in Bad Nauheim（Hessen）-Germany
Jurkat 細胞を、一次抗体に intracellular CD3 に対する抗体と Isotype、二次抗体に CF™ 488A および各社蛍光色素標識二次抗体を用いて染色しました。データ中の棒グラフは、Beckman Coulter FC-500 で分析した細胞集団の蛍光量の中央値の蛍光強度を測定した結果で、CF™ 488A 標識二次抗体は、他社製品と比較し、同等もしくは高い蛍光強度を持つことが示されています。ヒストグラムは、各社蛍光色素：CF™ 488A（緑）、A 社蛍光色素（青）、C 社蛍光色素（紫）、D 社蛍光色素（赤）を標識した二次抗体を用いて染色した細胞を、BD FACSCalibur™（FL1 チャネル）によって作成された各ヒストグラムを重ね合わせています。
使用例
CF™ Dye 標識体による多重染色
HeLa 細胞を、一次抗体に抗 golgin97 抗体（マウス）および抗 COX IV 抗体（ラビット）を用い、それぞれの二次抗体に CF™ 405S 標識の Goat Anti-Mouse IgG、CF™ 488A 標識の Goat Anti-Rabbit IgG を用いて染色しました。F-アクチンは CF™ 555 ファロイジンを用いて染色しました。
青：ゴルジ体、緑：ミトコンドリア、赤：細胞骨格
CF™ 555 ファロイジン、CF™ 488A 標識二次抗体を用いた染色
HeLa 細胞を、一次抗体に抗 α-チューブリン抗体（マウス）、二次抗体に CF™ 488A 標識の Goat Anti-Mouse IgG を用いて染色しました。F-アクチンは CF™ 555 ファロイジン、核の DNA は DAPI を用いて染色しました。
緑：チューブリン、赤：アクチン、青：核
価格表
| 製品名 | メーカー 製品番号 | 容量 | オンライン カタログへ |
|---|---|---|---|
| CF™488A Donkey Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20014-1 | 50 µL | |
| CF™488A Donkey Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20014 | 0.5 mL | |
| CF™488A Donkey Anti-Rabbit IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20015-1 | 50 µL | |
| CF™488A Donkey Anti-Rabbit IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20015 | 0.5 mL | |
| CF™488A Donkey Anti-Goat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20016-1 | 50 µL | |
| CF™488A Donkey Anti-Goat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20016 | 0.5 mL | |
| CF™488A Goat Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20018-1 | 50 µL | |
| CF™488A Goat Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20018 | 0.5 mL | |
| CF™488A Goat Anti-Rabbit IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20019-1 | 50 µL | |
| CF™488A Goat Anti-Rabbit IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20019 | 0.5 mL | |
| CF™488A Goat Anti-Chicken IgY(IgG)(H+L), Highly Cross-adsorbed, 2 mg/mL | 20020-1 | 50 µL | |
| CF™488A Goat Anti-Chicken IgY(IgG)(H+L), Highly Cross-adsorbed, 2 mg/mL | 20020 | 0.5 mL | |
| CF™488A Goat Anti-Human IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20022-1 | 50 µL | |
| CF™488A Goat Anti-Human IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20022 | 0.5 mL | |
| CF™488A Goat Anti-Rat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20023-1 | 50 µL | |
| CF™488A Goat Anti-Rat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20023 | 0.5 mL | |
| CF™488A Donkey Anti-sheep IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20024-1 | 50 µL | |
| CF™488A Donkey Anti-sheep IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20024 | 0.5 mL | |
| CF™488A Rabbit Anti-Rat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20025-1 | 50 µL | |
| CF™488A Rabbit Anti-Rat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20025 | 0.5 mL | |
| CF™488A Rabbit Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20026-1 | 50 µL | |
| CF™488A Rabbit Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20026 | 0.5 mL | |
| CF™488A Donkey Anti-Rat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20027-1 | 50 µL | |
| CF™488A Donkey Anti-Rat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20027 | 0.5 mL | |
| CF™488A Rabbit Anti-Human IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20071-1 | 50 µL | |
| CF™488A Rabbit Anti-Human IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20071 | 0.5 mL | |
| CF™488A Donkey Anti-Human IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20074-1 | 50 µL | |
| CF™488A Donkey Anti-Human IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20074 | 0.5 mL | |
| CF™488A Donkey Anti-Chicken IgY(IgG)(H+L), Highly Cross-adsorbed, 2 mg/mL | 20166-1 | 50 µL | |
| CF™488A Donkey Anti-Chicken IgY(IgG)(H+L), Highly Cross-adsorbed, 2 mg/mL | 20166 | 0.5 mL | |
| CF™488A Donkey Anti-Guinea Pig IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20169-1 | 50 µL | |
| CF™488A Donkey Anti-Guinea Pig IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20169 | 0.5 mL | |
| CF™488A Rabbit Anti-sheep IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20172-1 | 50 µL | |
| CF™488A Rabbit Anti-sheep IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20172 | 0.5 mL | |
| CF™488A Bovine Anti-Goat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20293-1 | 50 µL | |
| CF™488A Bovine Anti-Goat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20293 | 0.5 mL | |
| CF™488A Goat Anti-Mouse IgG(H+L), Highly Cross-adsorbed(min X Rat), 2 mg/mL | 20302-1 | 50 µL | |
| CF™488A Goat Anti-Mouse IgG(H+L), Highly Cross-adsorbed(min X Rat), 2 mg/mL | 20302 | 0.5 mL | |
| CF™488A Goat Anti-Mouse IgG(H+L), 2 mg/mL | 20010-1 | 50 µL | |
| CF™488A Goat Anti-Mouse IgG(H+L), 2 mg/mL | 20010 | 0.5 mL | |
| CF™488A Goat Anti-Rabbit IgG(H+L), 2 mg/mL | 20012-1 | 50 µL | |
| CF™488A Goat Anti-Rabbit IgG(H+L), 2 mg/mL | 20012 | 0.5 mL | |
| CF™488A Goat Anti-Guinea Pig IgG(H+L), 2 mg/mL | 20017-1 | 50 µL | |
| CF™488A Goat Anti-Guinea Pig IgG(H+L), 2 mg/mL | 20017 | 0.5 mL | |
| CF™488A Rabbit Anti-Goat IgG(H+L), 2 mg/mL | 20021-1 | 50 µL | |
| CF™488A Rabbit Anti-Goat IgG(H+L), 2 mg/mL | 20021 | 0.5 mL | |
| CF™488A Goat Anti-Swine IgG(H+L), 2 mg/mL | 20028-1 | 50 µL | |
| CF™488A Goat Anti-Swine IgG(H+L), 2 mg/mL | 20028 | 0.5 mL | |
| CF™488A Rabbit Anti-Chicken IgY(IgG)(H+L), 2 mg/mL | 20079-1 | 50 µL | |
| CF™488A Rabbit Anti-Chicken IgY(IgG)(H+L), 2 mg/mL | 20079 | 0.5 mL | |
| CF™488A Chicken Anti-Mouse IgG(H+L),2 mg/mL | 20208-1 | 50 µL | |
| CF™488A Chicken Anti-Mouse IgG(H+L),2 mg/mL | 20208 | 0.5 mL | |
| CF™488A Chicken Anti-Rabbit IgG(H+L),2 mg/mL | 20209-1 | 50 µL | |
| CF™488A Chicken Anti-Rabbit IgG(H+L),2 mg/mL | 20209 | 0.5 mL | |
| CF™488A Chicken Anti-Goat IgG(H+L), 2 mg/mL | 20225-1 | 50 µL | |
| CF™488A Chicken Anti-Goat IgG(H+L), 2 mg/mL | 20225 | 0.5 mL | |
| CF™488A Goat Anti-Mouse IgG1(γ1), 2 mg/mL | 20246-1 | 50 µL | |
| CF™488A Goat Anti-Mouse IgG1(γ1), 2 mg/mL | 20246 | 0.25 mL | |
| CF™488A Goat Anti-Mouse IgG2a(γ2a), 2 mg/mL | 20256-1 | 50 µL | |
| CF™488A Goat Anti-Mouse IgG2a(γ2a), 2 mg/mL | 20256 | 0.25 mL | |
| CF™488A Goat Anti-Mouse IgG2b(γ2b), 2 mg/mL | 20266-1 | 50 µL | |
| CF™488A Goat Anti-Mouse IgG2b(γ2b), 2 mg/mL | 20266 | 0.25 mL | |
| CF™488A Goat Anti-Human IgM(mu chain), 2 mg/mL | 20347-50uL | 50 µL | |
| CF™488A Goat Anti-Human IgM(mu chain), 2 mg/mL | 20347-250uL | 250 µL | |
| CF™488A F(ab')2 Fragment of Goat Anti-Mouse IgG(H+L), 2 mg/mL | 20011-1 | 50 µL | |
| CF™488A F(ab')2 Fragment of Goat Anti-Mouse IgG(H+L), 2 mg/mL | 20011 | 0.25 mL | |
| CF™488A F(ab')2 Fragment of Goat Anti-Rabbit IgG(H+L), 2 mg/mL | 20013 | 0.25 mL | |
| CF™488A Monoclonal Mouse Anti-Biotin IgG, 2 mg/mL | 20204-1 | 50 µL | |
| CF™488A Monoclonal Mouse Anti-Biotin IgG, 2 mg/mL | 20204 | 0.25 mL | |
| CF™488A Monoclonal Mouse Anti-Fluorescein(FITC) IgG, 2 mg/mL | 20210-1 | 50 µL | |
| CF™488A Monoclonal Mouse Anti-Fluorescein(FITC) IgG, 2 mg/mL | 20210 | 0.25 mL | |
| CF™488A Monoclonal Mouse Anti-GFP IgG, 1mg/ml | 20215 | 0.1 mL | |
| CF™488A Monoclonal Mouse Anti-6X His IgG, 1mg/ml | 20228 | 50 µL | |
| CF™488A Rabbit Anti-HA(Hemagglutinin) IgG, 1mg/ml | 20238 | 50 µL | |
| Annexin V, CF™488A Conjugate | 29005 | 0.5 mL | |
| Wheat Germ Agglutinin, CF™488A Conjugate | 29022-1 | 1 mg | |
| Wheat Germ Agglutinin, CF™488A Conjugate | 29022 | 5×1 mg | |
| Cholera Toxin Subunit B, CF™488A Conjugate | 00070 | 100 µg | |
| Concanavalin A, CF™488A Conjugate | 29016 | 5 mg | |
| Streptavidin, CF™488A Conjugate | 29034 | 1 mg | |
| Human Transferrin, CF™488A Conjugate | 00081 | 1 mg | |
| Phalloidin, CF™488A | 00042-T | 50 U | |
| Phalloidin, CF™488A | 00042 | 300 U | |
| α-Bungarotoxin, CF™488A | 00005 | 500 µg | |
| CF™488A-cAMP | 00036 | 100 µg | |
| CF™488A-dUTP, lyophilized powder | 40008 | 25 nmol | |
| CF™488A Azide | 92080 | 0.5 mg | |
| CF™488A, Aminooxy | 92051 | 1 mg | |
| CF™488A Amine | 92037 | 1 mg | |
| CF™488A Alkyne | 92086 | 0.5 mg | |
| CF™488A, Succinimidyl Ester | 92120 | 1 µmol | |
| CF™488A Hydrazide | 92152 | 1 mg | |
| CF™488A Maleimide | 92022 | 1 µmol | |
| Mix-n-Stain™ CF™488A Antibody Labeling Kit, 1×(5-20 µg) labeling | 92273 | 1 kit | |
| Mix-n-Stain™ CF™488A Antibody Labeling Kit, 1×(20-50 µg) labeling | 92253 | 1 kit | |
| Mix-n-Stain™ CF™488A Antibody Labeling Kit, 1×(50-100 µg) labeling | 92233 | 1 kit | |
| CF™488A Protein Labeling Kit 3 labelings | 92213 | 1 kit | |
| CF™488A and 7-AAD Apoptosis Assay Kit | 30060 | 1 kit | |
| CF™488A and PI Apoptosis Assay Kit | 30061 | 1 kit | |
| CF™488A TUNEL Assay Apoptosis Detection Kit | 30063 | 1 kit | |
CF™ 514
価格表
| 製品名 | メーカー 製品番号 | 容量 | オンライン カタログへ |
|---|---|---|---|
| CF™514 Goat Anti-Mouse IgG(H+L), 2 mg/mL | 20386-50uL | 50 µL | |
| CF™514 Goat Anti-Mouse IgG(H+L), 2 mg/mL | 20386-500uL | 0.5 mL | |
| CF™514 Goat Anti-Rabbit IgG(H+L), 2 mg/mL | 20387-50uL | 50 µL | |
| CF™514 Goat Anti-Rabbit IgG(H+L), 2 mg/mL | 20387-500uL | 0.5 mL | |
| CF™514 Succinimidyl Ester | 92103 | 1 µmol | |
CF™ 532
製品説明
CF™ 532 標識二次抗体の励起/蛍光スペクトル
Technical Summary
極大励起波長 / 極大蛍光波長：527 / 558 nm
分子吸光係数：96,000
分子量：～685
フローサイトメトリーレーザーライン：532 nm
顕微鏡レーザーライン：532 nm
CF™ 532 標識二次抗体の蛍光強度の比較
CF™ 532 Goat Anti-Mouse IgG は A 社製品と比較し、蛍光強度の強いことが示されています。
DOL：蛍光標識の程度
Jurkat 細胞染色における蛍光強度の比較
CF™ 532 Goat Anti-Mouse IgG または A 社製品で Jurkat 細胞を染色し、フローサイトメトリーにより蛍光強度を比較しました。CF™ Dye は A 社製品よりも蛍光強度の強いことが示されています。バックグラウンドは抗 CD3 抗体を加えていないサンプルにより測定しました。測定は BD FACSCalibur™ フローサイトメーター（FL2 チャンネル）を使用しました。
価格表
| 製品名 | メーカー 製品番号 | 容量 | オンライン カタログへ |
|---|---|---|---|
| CF™532 Goat Anti-Mouse IgG(H+L), 2 mg/mL | 20365-50uL | 50 µL | |
| CF™532 Goat Anti-Mouse IgG(H+L), 2 mg/mL | 20365-500uL | 0.5 mL | |
| CF™532 Goat Anti-Rabbit IgG(H+L), 2 mg/mL | 20366-50uL | 50 µL | |
| CF™532 Goat Anti-Rabbit IgG(H+L), 2 mg/mL | 20366-500uL | 0.5 mL | |
| Streptavidin, CF™532 Conjugate | 29030 | 1 mg | |
| Phalloidin, CF™532 | 00051-T | 50 U | |
| Phalloidin, CF™532 | 00051 | 300 U | |
| CF™532 Succinimidyl Ester | 92104 | 1 µmol | |
| CF™532 Maleimide | 92045 | 1 µmol | |
| Mix-n-Stain™ CF™532 Antibody Labeling Kit, 1×(5-20 µg) labeling | 92289 | 1 kit | |
| Mix-n-Stain™ CF™532 Antibody Labeling Kit, 1×(20-50 µg) labeling | 92290 | 1 kit | |
| Mix-n-Stain™ CF™532 Antibody Labeling Kit, 1×(50-100 µg) labeling | 92291 | 1 kit | |
| CF™532 Protein Labeling Kit 3 labelings | 92208 | 1 kit | |
CF™ 543
製品説明
CF™ 543 標識二次抗体の励起/蛍光スペクトル
Technical Summary
極大励起波長 / 極大蛍光波長：541 / 560 nm
分子吸光係数：100,000
分子量：〜870
フローサイトメトリーレーザーライン：532 nm
顕微鏡レーザーライン：543 or 546 nm
CF™ 543 を用いた染色
HeLa 細胞を、一次抗体にマウス抗チューブリン抗体、二次抗体に CF™ 543 標識の Goat Anti-mouse IgG を用いて染色しました。アクチンフィラメントと核は CF™ 488A ファロイジンと RedDot2 でそれぞれ対比染色しました。
CF™ 543 標識二次抗体の蛍光強度の比較
CF™ 543 Goat Anti-Mouse IgG は A 社製品と比較し、蛍光強度の強いことが示されています。
DOL：蛍光標識の程度
価格表
| 製品名 | メーカー 製品番号 | 容量 | オンライン カタログへ |
|---|---|---|---|
| CF™543 Goat Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20299-1 | 50 µL | |
| CF™543 Goat Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20299 | 0.5 mL | |
| CF™543 Goat Anti-Rabbit IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20300-1 | 50 µL | |
| CF™543 Goat Anti-Rabbit IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20300 | 0.5 mL | |
| CF™543 Donkey Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20305-1 | 50 µL | |
| CF™543 Donkey Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20305 | 0.5 mL | |
| CF™543 Rabbit Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20307-1 | 50 µL | |
| CF™543 Rabbit Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20307 | 0.5 mL | |
| CF™543 Donkey Anti-Rabbit IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20308-1 | 50 µL | |
| CF™543 Donkey Anti-Rabbit IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20308 | 0.5 mL | |
| CF™543 Donkey Anti-Chicken IgY(IgG)(H+L), Highly Cross-adsorbed, 2 mg/mL | 20310-1 | 50 µL | |
| CF™543 Donkey Anti-Chicken IgY(IgG)(H+L), Highly Cross-adsorbed, 2 mg/mL | 20310 | 0.5 mL | |
| CF™543 Goat Anti-Chicken IgY(H+L), Highly Cross-adsorbed, 2 mg/mL | 20311-1 | 50 µL | |
| CF™543 Goat Anti-Chicken IgY(H+L), Highly Cross-adsorbed, 2 mg/mL | 20311 | 0.5 mL | |
| CF™543 Bovine Anti-Goat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20313-1 | 50 µL | |
| CF™543 Bovine Anti-Goat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20313 | 0.5 mL | |
| CF™543 Donkey Anti-Goat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20314-1 | 50 µL | |
| CF™543 Donkey Anti-Goat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20314 | 0.5 mL | |
| CF™543 Donkey Anti-Guinea Pig IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20316-1 | 50 µL | |
| CF™543 Donkey Anti-Guinea Pig IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20316 | 0.5 mL | |
| CF™543 Donkey Anti-Human IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20318-1 | 50 µL | |
| CF™543 Donkey Anti-Human IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20318 | 0.5 mL | |
| CF™543 Goat Anti-Human IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20319-1 | 50 µL | |
| CF™543 Goat Anti-Human IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20319 | 0.5 mL | |
| CF™543 Donkey Anti-Rat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20320-1 | 50 µL | |
| CF™543 Donkey Anti-Rat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20320 | 0.5 mL | |
| CF™543 Goat Anti-Rat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20321-1 | 50 µL | |
| CF™543 Goat Anti-Rat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20321 | 0.5 mL | |
| CF™543 Donkey Anti-sheep IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20322-1 | 50 µL | |
| CF™543 Donkey Anti-sheep IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20322 | 0.5 mL | |
| CF™543 Rabbit Anti-sheep IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20323-1 | 50 µL | |
| CF™543 Rabbit Anti-sheep IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20323 | 0.5 mL | |
| CF™543 Goat Anti-Mouse IgG(H+L), Highly Cross-adsorbed(min X Rat), 2 mg/mL | 20328-1 | 50 µL | |
| CF™543 Goat Anti-Mouse IgG(H+L), Highly Cross-adsorbed(min X Rat), 2 mg/mL | 20328 | 0.5 mL | |
| CF™543 Goat Anti-Mouse IgG(H+L), 2 mg/mL | 20306-1 | 50 µL | |
| CF™543 Goat Anti-Mouse IgG(H+L), 2 mg/mL | 20306 | 0.5 mL | |
| CF™543 Goat Anti-Rabbit IgG(H+L), 2 mg/mL | 20309-1 | 50 µL | |
| CF™543 Goat Anti-Rabbit IgG(H+L), 2 mg/mL | 20309 | 0.5 mL | |
| CF™543 Rabbit Anti-Chicken IgY(IgG)(H+L), 2 mg/mL | 20312-1 | 50 µL | |
| CF™543 Rabbit Anti-Chicken IgY(IgG)(H+L), 2 mg/mL | 20312 | 0.5 mL | |
| CF™543 Rabbit Anti-Goat IgG(H+L), 2 mg/mL | 20315-1 | 50 µL | |
| CF™543 Rabbit Anti-Goat IgG(H+L), 2 mg/mL | 20315 | 0.5 mL | |
| CF™543 Goat Anti-Guinea Pig IgG(H+L), 2 mg/mL | 20317-1 | 50 µL | |
| CF™543 Goat Anti-Guinea Pig IgG(H+L), 2 mg/mL | 20317 | 0.5 mL | |
| CF™543 Goat Anti-Swine IgG(H+L), 2 mg/mL | 20324-1 | 50 µL | |
| CF™543 Goat Anti-Swine IgG(H+L), 2 mg/mL | 20324 | 0.5 mL | |
| CF™543 Goat Anti-Mouse IgG1(γ1), 2 mg/mL | 20325-1 | 50 µL | |
| CF™543 Goat Anti-Mouse IgG1(γ1), 2 mg/mL | 20325 | 0.25 mL | |
| CF™543 Goat Anti-Mouse IgG2a(γ2a), 2 mg/mL | 20326-1 | 50 µL | |
| CF™543 Goat Anti-Mouse IgG2a(γ2a), 2 mg/mL | 20326 | 0.25 mL | |
| CF™543 Goat Anti-Mouse IgG2b(γ2b), 2 mg/mL | 20327-1 | 50 µL | |
| CF™543 Goat Anti-Mouse IgG2b(γ2b), 2 mg/mL | 20327 | 0.25 mL | |
| CF™543 Chicken Anti-Goat IgG(H+L), 2 mg/mL | 20333-50uL | 50 µL | |
| CF™543 Chicken Anti-Goat IgG(H+L), 2 mg/mL | 20333-500uL | 0.5 mL | |
| CF™543 Chicken Anti-Mouse IgG(H+L), 2 mg/mL | 20334-50uL | 50 µL | |
| CF™543 Chicken Anti-Mouse IgG(H+L), 2 mg/mL | 20334-500uL | 0.5 mL | |
| CF™543 Chicken Anti-Rabbit IgG(H+L), 2 mg/mL | 20335-50uL | 50 µL | |
| CF™543 Chicken Anti-Rabbit IgG(H+L), 2 mg/mL | 20335-500uL | 0.5 mL | |
| CF™543 Rabbit Anit-Guinea Pig IgG(H+L), 2 mg/mL | 20336-50uL | 50 µL | |
| CF™543 Rabbit Anti-Guinea Pig IgG(H+L), 2 mg/mL | 20336-500uL | 0.5 mL | |
| CF™543 F(ab')2 Fragment of Goat Anti-Mouse IgG(H+L), 2 mg/mL | 20329-1 | 0.25 mL | |
| CF™543 F(ab')2 Fragment of Goat Anti-Mouse IgG(H+L), 2 mg/mL | 20329 | 0.25 mL | |
| CF™543 F(ab')2 Fragment of Goat Anti-Rabbit IgG(H+L), 2 mg/mL | 20330 | 0.25 mL | |
| Human Transferrin, CF™543 Conjugate | 00082 | 1 mg | |
| Phalloidin, CF™543 | 00043-T | 50 U | |
| Phalloidin, CF™543 | 00043 | 300 U | |
| α-Bungarotoxin, CF™543 | 00026 | 500 µg | |
| CF™543-dUTP, lyophilized powder | 40002 | 25 nmol | |
| CF™543 Succinimidyl Ester | 92105 | 1 µmol | |
| CF™543 Maleimide | 92044 | 1 µmol | |
| Mix-n-Stain™ CF™543 Antibody Labeling Kit, 1×(5-20 µg) labeling | 92287 | 1 kit | |
| Mix-n-Stain™ CF™543 Antibody Labeling Kit, 1×(20-50 µg) labeling | 92267 | 1 kit | |
| Mix-n-Stain™ CF™543 Antibody Labeling Kit, 1×(50-100 µg) labeling | 92247 | 1 kit | |
| CF™543 Protein Labeling Kit 3 labelings | 92209 | 1 kit | |
CF™ 555
製品説明
CF™ 555 標識二次抗体の励起/蛍光スペクトル
Technical Summary
極大励起波長 / 極大蛍光波長：555 / 565 nm
分子吸光係数：150,000
分子量：～901
フローサイトメトリーレーザーライン：532 nm
顕微鏡レーザーライン：543, 546 or 550 nm
CF™ 555 標識二次抗体を用いた染色
ラット脳の凍結切片のグリア細胞を、一次抗体に抗 GFAP 抗体（ラビット）、二次抗体に CF™ 555 標識 Donkey Anti-Rabbit IgG を用いて染色しました。核の DNA は DAPI を用いて染色しました。
CF™ Dye 標識体による多重染色
HeLa 細胞を、一次抗体に抗 golgin97 抗体（マウス）および抗 COX IV 抗体（ラビット）を用い、それぞれの二次抗体に CF™ 488A 標識の Goat Anti-Mouse IgG、CF™ 555 標識の Goat Anti-Rabbit IgG を用いて染色しました。F-アクチンは CF™ 647 ファロイジン、核の DNA は Hoechst 33342 を用いて染色しました。
緑：ゴルジ体、赤：ミトコンドリア、マゼンタ：細胞骨格、青：核
使用例
CF™ Dye 標識体による多重染色
HeLa 細胞を、一次抗体に抗 golgin97 抗体（マウス）および抗 COX IV 抗体（ラビット）を用い、それぞれの二次抗体に CF™ 405S 標識の Goat Anti-Mouse IgG、CF™ 488A 標識の Goat Anti-Rabbit IgG を用いて染色しました。F-アクチンは CF™ 555 ファロイジンを用いて染色しました。
青：ゴルジ体、緑：ミトコンドリア、赤：細胞骨格
CF™ 555 ファロイジン、CF™ 488A 標識二次抗体を用いた染色
HeLa 細胞を、一次抗体に抗 α-チューブリン抗体（マウス）、二次抗体に CF™ 488A 標識の Goat Anti-Mouse IgG を用いて染色しました。F-アクチンは CF™ 555 ファロイジン、核の DNA は DAPI を用いて染色しました。
緑：チューブリン、赤：アクチン、青：核
CF™ 555 および CF™ 647 標識二次抗体を用いたウェスタンブロッティング
マウス肝臓のライセートを二次元電気泳動を行った後、一次抗体に抗ヒトケラチン 8 抗体（ラビット）、抗ヒトケラチン 18 抗体（マウス）を用い、それぞれの二次抗体に CF™ 555 標識 Goat Anti-Rabbit IgG、CF™ 647 標識 Goat Anti-Mouse IgG を用いて検出しました。データは Cytiva Typhoon スキャナーにより検出しました。
価格表
| 製品名 | メーカー 製品番号 | 容量 | オンライン カタログへ |
|---|---|---|---|
| CF™555 Goat Anti-Chicken IgY(IgG)(H+L), Highly Cross-adsorbed, 2 mg/mL | 20034-1 | 50 µL | |
| CF™555 Goat Anti-Chicken IgY(IgG)(H+L), Highly Cross-adsorbed, 2 mg/mL | 20034 | 0.5 mL | |
| CF™555 Donkey Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20037-1 | 50 µL | |
| CF™555 Donkey Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20037 | 0.5 mL | |
| CF™555 Donkey Anti-Rabbit IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20038-1 | 50 µL | |
| CF™555 Donkey Anti-Rabbit IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20038 | 0.5 mL | |
| CF™555 Donkey Anti-Goat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20039-1 | 50 µL | |
| CF™555 Donkey Anti-Goat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20039 | 0.5 mL | |
| CF™555 Goat Anti-Human IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20230-1 | 50 µL | |
| CF™555 Goat Anti-Human IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20230 | 0.5 mL | |
| CF™555 Goat Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20231-1 | 50 µL | |
| CF™555 Goat Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20231 | 0.5 mL | |
| CF™555 Goat Anti-Rabbit IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20232-1 | 50 µL | |
| CF™555 Goat Anti-Rabbit IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20232 | 0.5 mL | |
| CF™555 Goat Anti-Rat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20233-1 | 50 µL | |
| CF™555 Goat Anti-Rat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20233 | 0.5 mL | |
| CF™555 Donkey Anti-sheep IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20234-1 | 50 µL | |
| CF™555 Donkey Anti-sheep IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20234 | 0.5 mL | |
| CF™555 Rabbit Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20235-1 | 50 µL | |
| CF™555 Rabbit Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20235 | 0.5 mL | |
| CF™555 Donkey Anti-Guinea Pig IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20276-1 | 50 µL | |
| CF™555 Donkey Anti-Guinea Pig IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20276 | 0.5 mL | |
| CF™555 Goat Anti-Mouse IgG(H+L), 2 mg/mL | 20030-1 | 50 µL | |
| CF™555 Goat Anti-Mouse IgG(H+L), 2 mg/mL | 20030 | 0.5 mL | |
| CF™555 Rabbit Anti-Goat IgG(H+L), 2 mg/mL | 20031-1 | 50 µL | |
| CF™555 Rabbit Anti-Goat IgG(H+L), 2 mg/mL | 20031 | 0.5 mL | |
| CF™555 Goat Anti-Rabbit IgG(H+L), 2 mg/mL | 20033-1 | 50 µL | |
| CF™555 Goat Anti-Rabbit IgG(H+L), 2 mg/mL | 20033 | 0.5 mL | |
| CF™555 Goat Anti-Guinea Pig IgG(H+L), 2 mg/mL | 20036-1 | 50 µL | |
| CF™555 Goat Anti-Guinea Pig IgG(H+L), 2 mg/mL | 20036 | 0.5 mL | |
| CF™555 Goat Anti-Swine IgG(H+L), 2 mg/mL | 20236-1 | 50 µL | |
| CF™555 Goat Anti-Swine IgG(H+L), 2 mg/mL | 20236 | 0.5 mL | |
| CF™555 Goat Anti-Mouse IgG1(γ1), 2 mg/mL | 20247-1 | 50 µL | |
| CF™555 Goat Anti-Mouse IgG1(γ1), 2 mg/mL | 20247 | 0.25 mL | |
| CF™555 Goat Anti-Mouse IgG2a(γ2a), 2 mg/mL | 20257-1 | 50 µL | |
| CF™555 Goat Anti-Mouse IgG2a(γ2a), 2 mg/mL | 20257 | 0.25 mL | |
| CF™555 Goat Anti-Mouse IgG2b(γ2b), 2 mg/mL | 20267-1 | 50 µL | |
| CF™555 Goat Anti-Mouse IgG2b(γ2b), 2 mg/mL | 20267 | 0.25 mL | |
| CF™555 F(ab')2 Fragment of Goat Anti-Mouse IgG(H+L), 2 mg/mL | 20032 | 0.25 mL | |
| CF™555 F(ab')2 Fragment of Goat Anti-Rabbit IgG(H+L), 2 mg/mL | 20035 | 0.25 mL | |
| Annexin V, CF™555 Conjugate | 29004 | 0.5 mL | |
| Streptavidin, CF™555 Conjugate | 29038 | 1 mg | |
| Phalloidin, CF™555 | 00040-T | 50 U | |
| Phalloidin, CF™555 | 00040 | 300 U | |
| α-Bungarotoxin, CF™555 | 00018 | 500 µg | |
| CF™555 Azide | 92081 | 0.5 mg | |
| CF™555 Amine | 92038 | 1 mg | |
| CF™555 Alkyne | 92087 | 0.5 mg | |
| CF™555, Succinimidyl Ester | 92130 | 1 µmol | |
| CF™555 Hydrazide | 92153 | 1 mg | |
| CF™555 Maleimide | 92023 | 1 µmol | |
| Mix-n-Stain™ CF™555 Antibody Labeling Kit, 1×(5-20 µg) labeling | 92274 | 1 kit | |
| Mix-n-Stain™ CF™555 Antibody Labeling Kit, 1×(20-50 µg) labeling | 92254 | 1 kit | |
| Mix-n-Stain™ CF™555 Antibody Labeling Kit, 1×(50-100 µg) labeling | 92234 | 1 kit | |
| CF™555 Protein Labeling Kit 3 labelings | 92214 | 1 kit | |
CF™ 568
製品説明
CF™ 568 標識二次抗体の励起/蛍光スペクトル
Technical Summary
極大励起波長 / 極大蛍光波長：562 / 583 nm
分子吸光係数：100,000
分子量：～714
フローサイトメトリーレーザーライン：532 nm
顕微鏡レーザーライン：568 nm
CF™ 568 標識二次抗体の蛍光強度の比較
Jurkat 細胞を、一次抗体に intracellular CD3 に対する抗体と Isotype、二次抗体に CF™ 568 および A 社蛍光色素標識の Goat Anti-Mouse IgG を用いて染色しました。蛍光強度は、BD FACSCalibur™（FL2 チャネル）により測定しました。その結果、CF™ 568 標識二次抗体は、A 社製品と比較し、高い蛍光強度を持つことが示されています。 DOL：蛍光標識の程度
CF™ 568 標識ストレプトアビジンの退色性の比較
Jurkat 細胞を、一次抗体にビオチン標識された intracellular CD3 に対する抗体を反応させた後、CF™ 568 および A 社蛍光色素標識のストレプトアビジンを用いて染色しました。データは水銀ランプを光源とする蛍光顕微鏡を用いて検出しました。15 秒間隔で 5 分間蛍光強度を測定した結果、CF™ 568 標識ストレプトアビジンは、退色し難いことが示されています。
価格表
| 製品名 | メーカー 製品番号 | 容量 | オンライン カタログへ |
|---|---|---|---|
| CF™568 Donkey Anti-Rat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20092-1 | 50 µL | |
| CF™568 Donkey Anti-Rat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20092 | 0.5 mL | |
| CF™568 Rabbit Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20093-1 | 50 µL | |
| CF™568 Rabbit Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20093 | 0.5 mL | |
| CF™568 Rabbit Anti-Rat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20094-1 | 50 µL | |
| CF™568 Rabbit Anti-Rat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20094 | 0.5 mL | |
| CF™568 Donkey Anti-sheep IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20095-1 | 50 µL | |
| CF™568 Donkey Anti-sheep IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20095 | 0.5 mL | |
| CF™568 Goat Anti-Rat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20096-1 | 50 µL | |
| CF™568 Goat Anti-Rat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20096 | 0.5 mL | |
| CF™568 Goat Anti-Human IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20097-1 | 50 µL | |
| CF™568 Goat Anti-Human IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20097 | 0.5 mL | |
| CF™568 Donkey Anti-Rabbit IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20098-1 | 50 µL | |
| CF™568 Donkey Anti-Rabbit IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20098 | 0.5 mL | |
| CF™568 Goat Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20101-1 | 50 µL | |
| CF™568 Goat Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20101 | 0.5 mL | |
| CF™568 Goat Anti-Rabbit IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20103-1 | 50 µL | |
| CF™568 Goat Anti-Rabbit IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20103 | 0.5 mL | |
| CF™568 Goat Anti-Chicken IgY(IgG)(H+L), Highly Cross-adsorbed, 2 mg/mL | 20104-1 | 50 µL | |
| CF™568 Goat Anti-Chicken IgY(IgG)(H+L), Highly Cross-adsorbed, 2 mg/mL | 20104 | 0.5 mL | |
| CF™568 Donkey Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20105-1 | 50 µL | |
| CF™568 Donkey Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20105 | 0.5 mL | |
| CF™568 Donkey Anti-Goat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20106-1 | 50 µL | |
| CF™568 Donkey Anti-Goat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20106 | 0.5 mL | |
| CF™568 Bovine Anti-Goat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20294-1 | 50 µL | |
| CF™568 Bovine Anti-Goat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20294 | 0.5 mL | |
| CF™568 Goat Anti-Mouse IgG(H+L), Highly Cross-adsorbed(min X Rat), 2 mg/mL | 20301-1 | 50 µL | |
| CF™568 Goat Anti-Mouse IgG(H+L), Highly Cross-adsorbed(min X Rat), 2 mg/mL | 20301 | 0.5 mL | |
| CF™568 Donkey Anti-Guinea Pig IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20377-50uL | 50 µL | |
| CF™568 Donkey Anti-Guinea Pig IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20377-500uL | 0.5 mL | |
| CF™568 Goat Anti-Swine IgG(H+L), 2 mg/mL | 20091-1 | 50 µL | |
| CF™568 Goat Anti-Swine IgG(H+L), 2 mg/mL | 20091 | 0.5 mL | |
| CF™568 Goat Anti-Mouse IgG(H+L), 2 mg/mL | 20100-1 | 50 µL | |
| CF™568 Goat Anti-Mouse IgG(H+L), 2 mg/mL | 20100 | 0.5 mL | |
| CF™568 Goat Anti-Rabbit IgG(H+L), 2 mg/mL | 20102-1 | 50 µL | |
| CF™568 Goat Anti-Rabbit IgG(H+L), 2 mg/mL | 20102 | 0.5 mL | |
| CF™568 Rabbit Anti-Goat IgG(H+L), 2 mg/mL | 20107-1 | 50 µL | |
| CF™568 Rabbit Anti-Goat IgG(H+L), 2 mg/mL | 20107 | 0.5 mL | |
| CF™568 Goat Anti-Guinea Pig IgG(H+L), 2 mg/mL | 20108-1 | 50 µL | |
| CF™568 Goat Anti-Guinea Pig IgG(H+L), 2 mg/mL | 20108 | 0.5 mL | |
| CF™568 Goat Anti-Mouse IgG1(γ1), 2 mg/mL | 20248-1 | 50 µL | |
| CF™568 Goat Anti-Mouse IgG1(γ1), 2 mg/mL | 20248 | 0.25 mL | |
| CF™568 Goat Anti-Mouse IgG2a(γ2a), 2 mg/mL | 20258-1 | 50 µL | |
| CF™568 Goat Anti-Mouse IgG2a(γ2a), 2 mg/mL | 20258 | 0.25 mL | |
| CF™568 Goat Anti-Mouse IgG2b(γ2b), 2 mg/mL | 20268-1 | 50 µL | |
| CF™568 Goat Anti-Mouse IgG2b(γ2b), 2 mg/mL | 20268 | 0.25 mL | |
| CF™568 Chicken Anti-Goat IgG(H+L), 2 mg/mL | 20337-50uL | 50 µL | |
| CF™568 Chicken Anti-Goat IgG(H+L), 2 mg/mL | 20337-500uL | 0.5 mL | |
| CF™568 Chicken Anti-Mouse IgG(H+L), 2 mg/mL | 20338-50uL | 50 µL | |
| CF™568 Chicken Anti-Mouse IgG(H+L), 2 mg/mL | 20338-500uL | 0.5 mL | |
| CF™568 Chicken Anti-Rabbit IgG(H+L), 2 mg/mL | 20339-50uL | 50 µL | |
| CF™568 Chicken Anti-Rabbit IgG(H+L), 2 mg/mL | 20339-500uL | 0.5 mL | |
| CF™568 F(ab')2 Fragment of Goat Anti-Mouse IgG(H+L), 2 mg/mL | 20109-1 | 50 µL | |
| Annexin V, CF™568 Conjugate | 29010 | 0.5 mL | |
| Cholera Toxin Subunit B, CF™568 Conjugate | 00071 | 100 µg | |
| Streptavidin, CF™568 Conjugate | 29035 | 1 mg | |
| Human Transferrin, CF™568 Conjugate | 00083 | 1 mg | |
| Phalloidin, CF™568 | 00044-T | 50 U | |
| Phalloidin, CF™568 | 00044 | 300 U | |
| α-Bungarotoxin, CF™568 | 00006 | 500 µg | |
| CF™568-dUTP | 40005 | 25 nmol | |
| CF™568 Azide | 92082 | 0.5 mg | |
| CF™568 Aminooxy | 92057 | 1 mg | |
| CF™568 Amine | 92039 | 1 mg | |
| CF™568 Alkyne | 92088 | 0.5 mg | |
| CF™568 Succinimidyl Ester | 92131 | 1 µmol | |
| CF™568 Hydrazide | 92154 | 1 mg | |
| CF™568 Maleimide | 92024 | 1 µmol | |
| Mix-n-Stain™ CF™568 Antibody Labeling Kit, 1×(5-20 µg) labeling | 92275 | 1 kit | |
| Mix-n-Stain™ CF™568 Antibody Labeling Kit, 1×(20-50 µg) labeling | 92255 | 1 kit | |
| Mix-n-Stain™ CF™568 Antibody Labeling Kit, 1×(50-100 µg) labeling | 92235 | 1 kit | |
| CF™568 Protein Labeling Kit 3 labelings | 92215 | 1 kit | |
CF™ 594
製品説明
CF™ 594 標識二次抗体の励起/蛍光スペクトル
Technical Summary
極大励起波長 / 極大蛍光波長：593 / 614 nm
分子吸光係数：115,000
分子量：〜730
フローサイトメトリーレーザーライン：532 or 594 nm
顕微鏡レーザーライン：594 nm
CF™ 594 標識二次抗体の蛍光強度の比較
CF™ 594 Goat Anti-Mouse IgG は、A 社製品と比較し、蛍光強度の強いことが示されています。
DOL：蛍光標識の程度
CF™ 594 標識二次抗体の退色性の比較
Jurkat 細胞を、一次抗体に intracellular CD3 に対する抗体、二次抗体に CF™ 594 および A 社蛍光色素標識の Goat Anti-Mouse IgG を用いて染色しました。データは水銀ランプを光源とする蛍光顕微鏡を用いて検出しました。15 秒間隔で 5 分間蛍光強度測定した結果、CF™ 594 標識二次抗体は、退色し難いことが示されています。
使用例
CF™ 594 標識二次抗体を用いた染色
HeLa 細胞を、一次抗体に抗 COX IV 抗体（ラビット）、二次抗体に CF™ 594 標識 Donkey Anti-Rabbit IgG を用いて染色しました。核の DNA は DAPI を用いて染色しました。データはオリンパス社の水銀ランプを光源とする顕微鏡により検出しました。
赤：ミトコンドリア、青：核
価格表
| 製品名 | メーカー 製品番号 | 容量 | オンライン カタログへ |
|---|---|---|---|
| CF™ 594 Rabbit Anti-Human IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20072-1 | 50 µL | |
| CF™ 594 Rabbit Anti-Human IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20072 | 0.5 mL | |
| CF™ 594 Donkey Anti-Human IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20075-1 | 50 µL | |
| CF™ 594 Donkey Anti-Human IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20075 | 0.5 mL | |
| CF™ 594 Goat Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20111-1 | 50 µL | |
| CF™ 594 Goat Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20111 | 0.5 mL | |
| CF™ 594 Goat Anti-Rabbit IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20113-1 | 50 µL | |
| CF™ 594 Goat Anti-Rabbit IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20113 | 0.5 mL | |
| CF™ 594 Goat Anti-Chicken IgY(IgG)(H+L), Highly Cross-adsorbed, 2 mg/mL | 20114-1 | 50 µL | |
| CF™ 594 Goat Anti-Chicken IgY(IgG)(H+L), Highly Cross-adsorbed, 2 mg/mL | 20114 | 0.5 mL | |
| CF™ 594 Donkey Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20115-1 | 50 µL | |
| CF™ 594 Donkey Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20115 | 0.5 mL | |
| CF™ 594 Donkey Anti-Goat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20116-1 | 50 µL | |
| CF™ 594 Donkey Anti-Goat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20116 | 0.5 mL | |
| CF™ 594 Donkey Anti-Rabbit IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20152-1 | 50 µL | |
| CF™ 594 Donkey Anti-Rabbit IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20152 | 0.5 mL | |
| CF™ 594 Goat Anti-Human IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20154-1 | 50 µL | |
| CF™ 594 Goat Anti-Human IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20154 | 0.5 mL | |
| CF™ 594 Goat Anti-Rat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20155-1 | 50 µL | |
| CF™ 594 Goat Anti-Rat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20155 | 0.5 mL | |
| CF™ 594 Donkey Anti-sheep IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20156-1 | 50 µL | |
| CF™ 594 Donkey Anti-sheep IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20156 | 0.5 mL | |
| CF™ 594 Rabbit Anti-Rat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20157-1 | 50 µL | |
| CF™ 594 Rabbit Anti-Rat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20157 | 0.5 mL | |
| CF™ 594 Rabbit Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20158-1 | 50 µL | |
| CF™ 594 Rabbit Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20158 | 0.5 mL | |
| CF™ 594 Donkey Anti-Rat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20159-1 | 50 µL | |
| CF™ 594 Donkey Anti-Rat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20159 | 0.5 mL | |
| CF™ 594 Donkey Anti-Chicken IgY(IgG)(H+L), Highly Cross-adsorbed, 2 mg/mL | 20167-1 | 50 µL | |
| CF™ 594 Donkey Anti-Chicken IgY(IgG)(H+L), Highly Cross-adsorbed, 2 mg/mL | 20167 | 0.5 mL | |
| CF™ 594 Donkey Anti-Guinea Pig IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20170-1 | 50 µL | |
| CF™ 594 Donkey Anti-Guinea Pig IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20170 | 0.5 mL | |
| CF™ 594 Rabbit Anti-sheep IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20173-1 | 50 µL | |
| CF™ 594 Rabbit Anti-sheep IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20173 | 0.5 mL | |
| CF™ 594 Bovine Anti-Goat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20295-1 | 50 µL | |
| CF™ 594 Bovine Anti-Goat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20295 | 0.5 mL | |
| CF™ 594 Goat Anti-Mouse IgG(H+L), Highly Cross-adsorbed(min X Rat), 2 mg/mL | 20303-1 | 50 µL | |
| CF™ 594 Goat Anti-Mouse IgG(H+L), Highly Cross-adsorbed(min X Rat), 2 mg/mL | 20303 | 0.5 mL | |
| CF™ 594 Goat Anti-Mouse IgG(H+L), 2 mg/mL | 20110-1 | 50 µL | |
| CF™ 594 Goat Anti-Mouse IgG(H+L), 2 mg/mL | 20110 | 0.5 mL | |
| CF™ 594 Goat Anti-Rabbit IgG(H+L), 2 mg/mL | 20112-1 | 50 µL | |
| CF™ 594 Goat Anti-Rabbit IgG(H+L), 2 mg/mL | 20112 | 0.5 mL | |
| CF™ 594 Rabbit Anti-Goat IgG(H+L), 2 mg/mL | 20117-1 | 50 µL | |
| CF™ 594 Rabbit Anti-Goat IgG(H+L), 2 mg/mL | 20117 | 0.5 mL | |
| CF™ 594 Goat Anti-Guinea Pig IgG(H+L), 2 mg/mL | 20118-1 | 50 µL | |
| CF™ 594 Goat Anti-Guinea Pig IgG(H+L), 2 mg/mL | 20118 | 0.5 mL | |
| CF™ 594 Goat Anti-Swine IgG(H+L), 2 mg/mL | 20160-1 | 50 µL | |
| CF™ 594 Goat Anti-Swine IgG(H+L), 2 mg/mL | 20160 | 0.5 mL | |
| CF™ 594 Rabbit Anti-Chicken IgY(IgG)(H+L), 2 mg/mL | 20164-1 | 50 µL | |
| CF™ 594 Rabbit Anti-Chicken IgY(IgG)(H+L), 2 mg/mL | 20164 | 0.5 mL | |
| CF™ 594 Chicken Anti-Mouse IgG(H+L), 2 mg/mL | 20221-1 | 50 µL | |
| CF™ 594 Chicken Anti-Mouse IgG(H+L), 2 mg/mL | 20221 | 0.5 mL | |
| CF™ 594 Chicken Anti-Rabbit IgG(H+L), 2 mg/mL | 20223-1 | 50 µL | |
| CF™ 594 Chicken Anti-Rabbit IgG(H+L), 2 mg/mL | 20223 | 0.5 mL | |
| CF™ 594 Chicken Anti-Goat IgG(H+L), 2 mg/mL | 20226-1 | 50 µL | |
| CF™ 594 Chicken Anti-Goat IgG(H+L), 2 mg/mL | 20226 | 0.5 mL | |
| CF™ 594 Goat Anti-Mouse IgG1(γ1), 2 mg/mL | 20249-1 | 50 µL | |
| CF™ 594 Goat Anti-Mouse IgG1(γ1), 2 mg/mL | 20249 | 0.25 mL | |
| CF™ 594 Goat Anti-Mouse IgG2a(γ2a), 2 mg/mL | 20259-1 | 50 µL | |
| CF™ 594 Goat Anti-Mouse IgG2a(γ2a), 2 mg/mL | 20259 | 0.25 mL | |
| CF™ 594 Goat Anti-Mouse IgG2b(γ2b), 2 mg/mL | 20269-1 | 50 µL | |
| CF™ 594 Goat Anti-Mouse IgG2b(γ2b), 2 mg/mL | 20269 | 0.25 mL | |
| CF™ 594 Goat Anti-Human IgM(mu chain), 2 mg/mL | 20348-50uL | 50 µL | |
| CF™ 594 Goat Anti-Human IgM(mu chain), 2 mg/mL | 20348-250uL | 250 µL | |
| CF™ 594 F(ab')2 Fragment of Goat Anti-Mouse IgG(H+L), 2 mg/mL | 20119-1 | 50 µL | |
| CF™ 594 F(ab')2 Fragment of Goat Anti-Mouse IgG(H+L), 2 mg/mL | 20119 | 0.25 mL | |
| CF™ 594 F(ab')2 Fragment of Goat Anti-Rabbit IgG(H+L), 2 mg/mL | 20153 | 0.25 mL | |
| CF™ 594 Monoclonal Mouse Anti-Biotin IgG, 2 mg/mL | 20205-1 | 50 µL | |
| CF™ 594 Monoclonal Mouse Anti-Biotin IgG, 2 mg/mL | 20205 | 0.25 mL | |
| CF™ 594 Monoclonal Mouse Anti-Fluorescein(FITC) IgG, 2 mg/mL | 20211-1 | 50 µL | |
| CF™ 594 Monoclonal Mouse Anti-Fluorescein(FITC) IgG, 2 mg/mL | 20211 | 0.25 mL | |
| CF™ 594 Monoclonal Mouse Anti-GFP IgG, 1mg/ml | 20216 | 0.1 mL | |
| CF™ 594 Monoclonal Mouse Anti-6X His IgG, 1 mg/ml | 20229 | 50 µL | |
| CF™ 594 Rabbit Anti-HA(Hemagglutinin)IgG, 1 mg/ml | 20239 | 50 µL | |
| Annexin V, CF™ 594 Conjugate | 29011 | 0.5 mL | |
| Wheat Germ Agglutinin, CF™ 594 Conjugate | 29023-1 | 1 mg | |
| Wheat Germ Agglutinin, CF™ 594 Conjugate | 29023 | 5×1 mg | |
| Cholera Toxin Subunit B, CF™ 594 Conjugate | 00072 | 100 µg | |
| Concanavalin A, CF™ 594 Conjugate | 29017 | 5 mg | |
| Streptavidin, CF™ 594 Conjugate | 29036 | 1 mg | |
| Human Transferrin, CF™ 594 Conjugate | 00084 | 1 mg | |
| Phalloidin, CF™ 594 | 00045-T | 50 U | |
| Phalloidin, CF™ 594 | 00045 | 300 U | |
| α-Bungarotoxin, CF™ 594 | 00007 | 500 µg | |
| CF™ 594-dUTP | 40006 | 25 nmol | |
| CF™ 594 Azide | 92083 | 0.5 mg | |
| CF™ 594, Aminooxy | 92052 | 1 mg | |
| CF™ 594 Amine | 92040 | 1 mg | |
| CF™ 594 Alkyne | 92089 | 0.5 mg | |
| CF™ 594 Succinimidyl Ester | 92132 | 1 µmol | |
| CF™ 594 Hydrazide | 92158 | 1 mg | |
| CF™ 594 Maleimide | 92025 | 1 µmol | |
| Mix-n-Stain™ CF™ 594 Antibody Labeling Kit, 1×(5-20 µg) labeling | 92276 | 1 kit | |
| Mix-n-Stain™ CF™ 594 Antibody Labeling Kit, 1×(20-50 µg) labeling | 92256 | 1 kit | |
| Mix-n-Stain™ CF™ 594 Antibody Labeling Kit, 1×(50-100 µg) labeling | 92236 | 1 kit | |
| CF™ 594 Protein Labeling Kit 3 labelings | 92216 | 1 kit | |
| CF™ 594 TUNEL Assay Apoptosis Detection Kit | 30064 | 1 kit | |
| Dual Apoptosis Assay with NucView 488 Caspase-3 Substrate and CF™ 594-Annexin V (50 assays) | 30067 | 1 kit | |
CF™ 620R
価格表
| 製品名 | メーカー 製品番号 | 容量 | オンライン カタログへ |
|---|---|---|---|
| CF™620R Succinimidyl Ester | 92106 | 1 µmol | |
| CF™620R Maleimide | 92033 | 1 µmol | |
CF™ 633
製品説明
CF™ 633 標識二次抗体の励起/蛍光スペクトル
Technical Summary
極大励起波長 / 極大蛍光波長：630 / 650 nm
分子吸光係数：100,000
分子量：～820
フローサイトメトリーレーザーライン：633 or 635 nm
顕微鏡レーザーライン：633 or 635 nm
CF™ 633 標識二次抗体の蛍光強度の比較
Jurkat 細胞を、一次抗体に intracellular CD3 に対する抗体と Isotype、二次抗体に CF™ 633 および A 社の 2 種類の蛍光色素標識の Goat Anti-Mouse IgG を用いて染色しました。蛍光強度は、BD FACSCalibur™（FL4 チャネル）により測定しました。グラフは細胞集団の蛍光強度の相乗平均を表しています。その結果、CF™ 633 標識二次抗体は、A 社の 2 製品と比較し、高い蛍光強度を持つことが示されています。
DOL：蛍光標識の程度
CF™ 633 標識二次抗体の退色性の比較
Jurkat 細胞を、一次抗体に CD3 に対する抗体、二次抗体に CF™ 633 および A 社蛍光色素標識の Goat Anti-Rabbit IgG を用いて染色しました。データはオリンパス社の水銀ランプを光源とする顕微鏡により、Cy5 フィルター、CCD カメラを用いて検出しました。蛍光顕微鏡で検出した結果、CF™ 633 で標識した抗体は、退色時間の長いことが示されています。
価格表
| 製品名 | メーカー 製品番号 | 容量 | オンライン カタログへ |
|---|---|---|---|
| CF™633 Rabbit Anti-Human IgG(H+L), Highly Cross-adsorbed | 20066-1 | 50 µL | |
| CF™633 Rabbit Anti-Human IgG(H+L), Highly Cross-adsorbed | 20066 | 0.5 mL | |
| CF™633 Donkey Anti-Human IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20076-1 | 50 µL | |
| CF™633 Donkey Anti-Human IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20076 | 0.5 mL | |
| CF™633 Goat Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20121-1 | 50 µL | |
| CF™633 Goat Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20121 | 0.5 mL | |
| CF™633 Goat Anti-Rabbit IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20123-1 | 50 µL | |
| CF™633 Goat Anti-Rabbit IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20123 | 0.5 mL | |
| CF™633 Donkey Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20124-1 | 50 µL | |
| CF™633 Donkey Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20124 | 0.5 mL | |
| CF™633 Donkey Anti-Rabbit IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20125-1 | 50 µL | |
| CF™633 Donkey Anti-Rabbit IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20125 | 0.5 mL | |
| CF™633 Goat Anti-Chicken IgY(IgG)(H+L), Highly Cross-adsorbed, 2 mg/mL | 20126-1 | 50 µL | |
| CF™633 Goat Anti-Chicken IgY(IgG)(H+L), Highly Cross-adsorbed, 2 mg/mL | 20126 | 0.5 mL | |
| CF™633 Donkey Anti-Goat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20127-1 | 50 µL | |
| CF™633 Donkey Anti-Goat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20127 | 0.5 mL | |
| CF™633 Goat Anti-Human IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20132-1 | 50 µL | |
| CF™633 Goat Anti-Human IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20132 | 0.5 mL | |
| CF™633 Goat Anti-Rat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20133-1 | 50 µL | |
| CF™633 Goat Anti-Rat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20133 | 0.5 mL | |
| CF™633 Donkey Anti-sheep IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20134-1 | 50 µL | |
| CF™633 Donkey Anti-sheep IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20134 | 0.5 mL | |
| CF™633 Rabbit Anti-Rat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20135-1 | 50 µL | |
| CF™633 Rabbit Anti-Rat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20135 | 0.5 mL | |
| CF™633 Rabbit Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20136-1 | 50 µL | |
| CF™633 Rabbit Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20136 | 0.5 mL | |
| CF™633 Donkey Anti-Rat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20137-1 | 50 µL | |
| CF™633 Donkey Anti-Rat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20137 | 0.5 mL | |
| CF™633 Donkey Anti-Chicken IgY(IgG)(H+L), Highly Cross-adsorbed, 2 mg/mL | 20168-1 | 50 µL | |
| CF™633 Donkey Anti-Chicken IgY(IgG)(H+L), Highly Cross-adsorbed, 2 mg/mL | 20168 | 0.5 mL | |
| CF™633 Donkey Anti-Guinea Pig IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20171-1 | 50 µL | |
| CF™633 Donkey Anti-Guinea Pig IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20171 | 0.5 mL | |
| CF™633 Rabbit Anti-sheep IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20174-1 | 50 µL | |
| CF™633 Rabbit Anti-sheep IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20174 | 0.5 mL | |
| CF™633 Bovine Anti-Goat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20296-1 | 50 µL | |
| CF™633 Bovine Anti-Goat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20296 | 0.5 mL | |
| CF™633 Goat Anti-Mouse IgG(H+L), Highly Cross-adsorbed(min X Rat), 2 mg/mL | 20341-50uL | 50 µL | |
| CF™633 Goat Anti-Mouse IgG(H+L), Highly Cross-adsorbed(min X Rat), 2 mg/mL | 20341-500uL | 0.5 mL | |
| CF™633 Goat Anti-Mouse IgG(H+L), 2 mg/mL | 20120-1 | 50 µL | |
| CF™633 Goat Anti-Mouse IgG(H+L), 2 mg/mL | 20120 | 0.5 mL | |
| CF™633 Goat Anti-Rabbit IgG(H+L), 2 mg/mL | 20122-1 | 50 µL | |
| CF™633 Goat Anti-Rabbit IgG(H+L), 2 mg/mL | 20122 | 0.5 mL | |
| CF™633 Rabbit Anti-Goat IgG(H+L), 2 mg/mL | 20128-1 | 50 µL | |
| CF™633 Rabbit Anti-Goat IgG(H+L), 2 mg/mL | 20128 | 0.5 mL | |
| CF™633 Goat Anti-Guinea Pig IgG(H+L), 2 mg/mL | 20129-1 | 50 µL | |
| CF™633 Goat Anti-Guinea Pig IgG(H+L), 2 mg/mL | 20129 | 0.5 mL | |
| CF™633 Goat Anti-Swine IgG(H+L), 2 mg/mL | 20138-1 | 50 µL | |
| CF™633 Goat Anti-Swine IgG(H+L), 2 mg/mL | 20138 | 0.5 mL | |
| CF™633 Rabbit Anti-Chicken IgY(IgG)(H+L), 2 mg/mL | 20165-1 | 50 µL | |
| CF™633 Rabbit Anti-Chicken IgY(IgG)(H+L), 2 mg/mL | 20165 | 0.5 mL | |
| CF™633 Chicken Anti-Mouse IgG(H+L), 2 mg/mL | 20222-1 | 50 µL | |
| CF™633 Chicken Anti-Mouse IgG(H+L), 2 mg/mL | 20222 | 0.5 mL | |
| CF™633 Chicken Anti-Rabbit IgG(H+L), 2 mg/mL | 20224-1 | 50 µL | |
| CF™633 Chicken Anti-Rabbit IgG(H+L), 2 mg/mL | 20224 | 0.5 mL | |
| CF™633 Chicken Anti-Goat IgG(H+L), 2 mg/mL | 20227-1 | 50 µL | |
| CF™633 Chicken Anti-Goat IgG(H+L), 2 mg/mL | 20227 | 0.5 mL | |
| CF™633 Goat Anti-Mouse IgG1(γ1), 2 mg/mL | 20250-1 | 50 µL | |
| CF™633 Goat Anti-Mouse IgG1(γ1), 2 mg/mL | 20250 | 0.25 mL | |
| CF™633 Goat Anti-Mouse IgG2a(γ2a), 2 mg/mL | 20260-1 | 50 µL | |
| CF™633 Goat Anti-Mouse IgG2a(γ2a), 2 mg/mL | 20260 | 0.25 mL | |
| CF™633 Goat Anti-Mouse IgG2b(γ2b), 2 mg/mL | 20270-1 | 50 µL | |
| CF™633 Goat Anti-Mouse IgG2b(γ2b), 2 mg/mL | 20270 | 0.25 mL | |
| CF™633 F(ab')2 Fragment of Goat Anti-Mouse IgG(H+L), 2 mg/mL | 20130 | 0.25 mL | |
| CF™633 F(ab')2 Fragment of Goat Anti-Rabbit IgG(H+L), 2 mg/mL | 20131 | 0.25 mL | |
| CF™633 Monoclonal Mouse Anti-Biotin IgG, 2 mg/mL | 20206-1 | 50 µL | |
| CF™633 Monoclonal Mouse Anti-Biotin IgG, 2 mg/mL | 20206 | 0.25 mL | |
| CF™633 Monoclonal Mouse Anti-Fluorescein(FITC) IgG, 2 mg/mL | 20212-1 | 50 µL | |
| CF™633 Monoclonal Mouse Anti-Fluorescein(FITC) IgG, 2 mg/mL | 20212 | 0.25 mL | |
| CF™633 Monoclonal Mouse Anti-GFP IgG, 1mg/ml | 20217 | 0.1 mL | |
| Annexin V, CF™633 Conjugate | 29008 | 0.5 mL | |
| Wheat Germ Agglutinin, CF™633 Conjugate | 29024-1 | 1 mg | |
| Wheat Germ Agglutinin, CF™633 Conjugate | 29024 | 5×1 mg | |
| Concanavalin A, CF™633 Conjugate | 29018 | 5 mg | |
| Streptavidin, CF™633 Conjugate | 29037 | 1 mg | |
| Phalloidin, CF™633 | 00046-T | 50 U | |
| Phalloidin, CF™633 | 00046 | 300 U | |
| α-Bungarotoxin, CF™633 | 00009 | 500 µg | |
| CF™633, Aminooxy | 92053 | 1 mg | |
| CF™633 Amine | 92041 | 1 mg | |
| CF™633 Succinimidyl Ester | 92133 | 1 µmol | |
| CF™633 Hydrazide | 92156 | 1 mg | |
| CF™633 Maleimide | 92026 | 1 µmol | |
| Mix-n-Stain™ CF™633 Antibody Labeling Kit, 1×(5-20 µg) labeling | 92277 | 1 kit | |
| Mix-n-Stain™ CF™633 Antibody Labeling Kit, 1×(20-50 µg) labeling | 92257 | 1 kit | |
| Mix-n-Stain™ CF™633 Antibody Labeling Kit, 1×(50-100 µg) labeling | 92237 | 1 kit | |
| CF™633 Protein Labeling Kit 3 labelings | 92217 | 1 kit | |
CF™ 640R
製品説明
CF™ 640R 標識二次抗体の励起/蛍光スペクトル
Technical Summary
極大励起波長 / 極大蛍光波長：642 / 662 nm
分子吸光係数：105,000
分子量：～832
フローサイトメトリーレーザーライン：633, 635 or 640 nm
顕微鏡レーザーライン：633, 635 or 640 nm
CF™ 640R 標識二次抗体の蛍光強度の比較
Jurkat 細胞を、一次抗体に intracellular CD3 に対する抗体、二次抗体に CF™ 640R、A 社、B 社、2 種類の E 社の蛍光色素標識の Goat Anti-Mouse IgG を用いて染色しました。コントロールは、二次抗体のみを用いて検出しました。蛍光は、BD FACSCalibur™（FL4 チャネル）により測定しました。棒グラフは、細胞集団の蛍光強度の相乗平均を示しています。
DOL：蛍光標識の程度
CF™ 640R 色素の退色性の比較
PBS に 0.5 mM の濃度で CF™ 640R および E 社の蛍光色素を溶解し、水銀ランプを光源とする蛍光顕微鏡で Cy5 フィルターを用いて検出しました。15 秒間隔で 5 分間蛍光強度を測定した結果、CF™ 640R は、退色し難いことが示されています。蛍光の強度は、Image-Pro Express software を用いて算出されています。
価格表
| 製品名 | メーカー 製品番号 | 容量 | オンライン カタログへ |
|---|---|---|---|
| CF™640R Goat Anti-Human IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20081-1 | 50 µL | |
| CF™640R Goat Anti-Human IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20081 | 0.5 mL | |
| CF™640R Donkey Anti-sheep IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20083-1 | 50 µL | |
| CF™640R Donkey Anti-sheep IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20083 | 0.5 mL | |
| CF™640R Goat Anti-Chicken IgY(IgG)(H+L), Highly Cross-adsorbed, 2 mg/mL | 20084-1 | 50 µL | |
| CF™640R Goat Anti-Chicken IgY(IgG)(H+L), Highly Cross-adsorbed, 2 mg/mL | 20084 | 0.5 mL | |
| CF™640R Goat Anti-Rat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20088-1 | 50 µL | |
| CF™640R Goat Anti-Rat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20088 | 0.5 mL | |
| CF™640R Goat Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20175-1 | 50 µL | |
| CF™640R Goat Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20175 | 0.5 mL | |
| CF™640R Goat Anti-Rabbit IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20176-1 | 50 µL | |
| CF™640R Goat Anti-Rabbit IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20176 | 0.5 mL | |
| CF™640R Donkey Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20177-1 | 50 µL | |
| CF™640R Donkey Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20177 | 0.5 mL | |
| CF™640R Donkey Anti-Rabbit IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20178-1 | 50 µL | |
| CF™640R Donkey Anti-Rabbit IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20178 | 0.5 mL | |
| CF™640R Donkey Anti-Goat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20179-1 | 50 µL | |
| CF™640R Donkey Anti-Goat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20179 | 0.5 mL | |
| CF™640R Donkey Anti-Rat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20199-1 | 50 µL | |
| CF™640R Donkey Anti-Rat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20199 | 0.5 mL | |
| CF™640R Rabbit Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20200-1 | 50 µL | |
| CF™640R Rabbit Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20200 | 0.5 mL | |
| CF™640R Rabbit Anti-Rat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20201-1 | 50 µL | |
| CF™640R Rabbit Anti-Rat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20201 | 0.5 mL | |
| CF™640R Bovine Anti-Goat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20297-1 | 50 µL | |
| CF™640R Bovine Anti-Goat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20297 | 0.5 mL | |
| CF™640R Goat Anti-Mouse IgG(H+L), Highly Cross-adsorbed(min X Rat), 2 mg/mL | 20304-1 | 50 µL | |
| CF™640R Goat Anti-Mouse IgG(H+L), Highly Cross-adsorbed(min X Rat), 2 mg/mL | 20304 | 0.5 mL | |
| CF™640R Goat Anti-Guinea Pig IgG(H+L), 2 mg/mL | 20085-1 | 50 µL | |
| CF™640R Goat Anti-Guinea Pig IgG(H+L), 2 mg/mL | 20085 | 0.5 mL | |
| CF™640R Goat Anti-Swine IgG(H+L), 2 mg/mL | 20089-1 | 50 µL | |
| CF™640R Goat Anti-Swine IgG(H+L), 2 mg/mL | 20089 | 0.5 mL | |
| CF™640R Rabbit Anti-Goat IgG(H+L), 2 mg/mL | 20090-1 | 50 µL | |
| CF™640R Rabbit Anti-Goat IgG(H+L), 2 mg/mL | 20090 | 0.5 mL | |
| CF™640R Goat Anti-Mouse IgG(H+L), 2 mg/mL | 20197-1 | 50 µL | |
| CF™640R Goat Anti-Mouse IgG(H+L), 2 mg/mL | 20197 | 0.5 mL | |
| CF™640R Goat Anti-Rabbit IgG(H+L), 2 mg/mL | 20202-1 | 50 µL | |
| CF™640R Goat Anti-Rabbit IgG(H+L), 2 mg/mL | 20202 | 0.5 mL | |
| CF™640R Goat Anti-Mouse IgG1(γ1), 2 mg/mL | 20251-1 | 50 µL | |
| CF™640R Goat Anti-Mouse IgG1(γ1), 2 mg/mL | 20251 | 0.25 mL | |
| CF™640R Goat Anti-Mouse IgG2a(γ2a), 2 mg/mL | 20261-1 | 50 µL | |
| CF™640R Goat Anti-Mouse IgG2a(γ2a), 2 mg/mL | 20261 | 0.25 mL | |
| CF™640R Goat Anti-Mouse IgG2b(γ2b), 2 mg/mL | 20271-1 | 50 µL | |
| CF™640R Goat Anti-Mouse IgG2b(γ2b), 2 mg/mL | 20271 | 0.25 mL | |
| CF™640R Goat Anti-Human IgM(mu chain), 2 mg/mL | 20349-50uL | 50 µL | |
| CF™640R Goat Anti-Human IgM(mu chain), 2 mg/mL | 20349-250uL | 250 µL | |
| CF™640R F(ab')2 Fragment of Goat Anti-Mouse IgG(H+L), 2 mg/mL | 20086 | 0.25 mL | |
| CF™640R F(ab')2 Fragment of Goat Anti-Rabbit IgG(H+L), 2 mg/mL | 20087 | 0.25 mL | |
| CF™640R Monoclonal Mouse Anti-Biotin IgG, 2 mg/mL | 20207-1 | 50 µL | |
| CF™640R Monoclonal Mouse Anti-Biotin IgG, 2 mg/mL | 20207 | 0.25 mL | |
| CF™640R Monoclonal Mouse Anti-Fluorescein(FITC) IgG, 2 mg/mL | 20213-1 | 50 µL | |
| CF™640R Monoclonal Mouse Anti-Fluorescein(FITC) IgG, 2 mg/mL | 20213 | 0.25 mL | |
| CF™640R Monoclonal Mouse Anti-GFP IgG, 1mg/ml | 20218 | 0.1 mL | |
| CF™640R Monoclonal Mouse Anti-6X His IgG, 1mg/ml | 20237 | 50 µL | |
| CF™640R Rabbit Anti-HA(Hemagglutinin)IgG, 1mg/ml | 20240 | 50 µL | |
| Annexin V, CF™640R Conjugate | 29014 | 0.5 mL | |
| Wheat Germ Agglutinin, CF™640R Conjugate | 29026-1 | 1 mg | |
| Wheat Germ Agglutinin, CF™640R Conjugate | 29026 | 5×1 mg | |
| Cholera Toxin Subunit B, CF™640R Conjugate | 00073 | 100 µg | |
| Concanavalin A, CF™640R Conjugate | 29019 | 5 mg | |
| Streptavidin, CF™640R Conjugate | 29041 | 1 mg | |
| Human Transferrin, CF™640R Conjugate | 00085 | 1 mg | |
| Phalloidin, CF™640R | 00050-T | 50 U | |
| Phalloidin, CF™640R | 00050 | 300 U | |
| α-Bungarotoxin, CF™640R | 00004 | 500 µg | |
| CF™640R-cAMP | 00037 | 100 µg | |
| CF™640R-dUTP, lyophilized powder | 40007 | 25 nmol | |
| CF™640R Azide | 92085 | 0.5 mg | |
| CF™640R Aminooxy | 92058 | 1 mg | |
| CF™640R Amine | 92043 | 1 mg | |
| CF™640R Alkyne | 92091 | 0.5 mg | |
| CF™640R Succinimidyl Ester | 92108 | 1 µmol | |
| CF™640R Hydrazide | 92157 | 1 mg | |
| CF™640R Maleimide | 92034 | 1 µmol | |
| Mix-n-Stain™ CF™640R Antibody Labeling Kit, 1×(5-20 µg) labeling | 92278 | 1 kit | |
| Mix-n-Stain™ CF™640R Antibody Labeling Kit, 1×(20-50 µg) labeling | 92258 | 1 kit | |
| Mix-n-Stain™ CF™640R Antibody Labeling Kit, 1×(50-100 µg) labeling | 92245 | 1 kit | |
| CF™640R Protein Labeling Kit 3 labelings | 92225 | 1 kit | |
CF™ 647
製品説明
CF™ 647 標識二次抗体の励起/蛍光スペクトル
Technical Summary
極大励起波長 / 極大蛍光波長：650 / 665 nm
分子吸光係数：240,000
分子量：～836
フローサイトメトリーレーザーライン：633 or 635 nm
顕微鏡レーザーライン：633 or 635 nm
CF™ 647 標識二次抗体の S/N 比の比較
Jurkat 細胞を、一次抗体にマウス抗ヒト CD3 抗体、二次抗体に CF™ 647、A 社色素、B 社色素標識の Goat Anti-Mouse IgG をそれぞれ用いて染色し、BD FACS Calibur（FL4チャネル）により蛍光強度を測定して、S/N 比を算出しました。その結果、CF™ 647 標識二次抗体は最も高い S/N 比を得ることができました。なお、二次抗体のみのシグナルをバックグラウンド（2nd）としています。
DOL：蛍光標識の程度
CF™ 647 標識アネキシンVを用いた染色
Jurkat 細胞を CF™ 647 標識アネキシンV（紫）で染色しました。この細胞はアポトーシス誘導されており、NucView™488（緑）でカスパーゼ 3 の活性を検出しています。
使用例
CF™ 555 および CF™ 647 標識二次抗体を用いたウェスタンブロッティング
マウス肝臓のライセートを二次元電気泳動を行った後、一次抗体に抗ヒトケラチン 8 抗体（ラビット）、抗ヒトケラチン 18 抗体（マウス）を用い、それぞれの二次抗体に CF™ 555 標識 Goat Anti-Rabbit IgG、CF™ 647 標識 Goat Anti-Mouse IgG を用いて検出しました。データは Cytiva Typhoon スキャナーにより検出しました。
CF™ 647 ヒドラジドを用いた神経海馬の染色
ラットの神経海馬を、SynaptoGreen™ C4（#70020）とCF™ 647 Hydrazide を用いて染色しました。SynaptoGreen™ C4 はシナプス小胞を緑に染色し、CF™ 647 Hydrazide は、細胞体を赤に染色しました。
データ提供：Hang Zhou from Professor Guosong Liu's lab in Tsinghua University, China
CF™ 647 標識二次抗体を用いた染色
HeLa 細胞を、一次抗体に抗 α-チューブリン抗体（マウス）、二次抗体に CF™ 647 標識 Goat Anti-Mouse IgG F(ab')2 fragment を用いて染色しました。データはオリンパス社の水銀ランプを光源とする顕微鏡により、Cy5 フィルター、CCD カメラ、Image-Pro Express software を用いて検出しました。
価格表
| 製品名 | メーカー 製品番号 | 容量 | オンライン カタログへ |
|---|---|---|---|
| CF™647 Goat Anti-Chicken IgY(IgG)(H+L), Highly Cross-adsorbed, 2 mg/mL | 20044-1 | 50 µL | |
| CF™647 Goat Anti-Chicken IgY(IgG)(H+L), Highly Cross-adsorbed, 2 mg/mL | 20044 | 0.5 mL | |
| CF™647 Donkey Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20046-1 | 50 µL | |
| CF™647 Donkey Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20046 | 0.5 mL | |
| CF™647 Donkey Anti-Rabbit IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20047-1 | 50 µL | |
| CF™647 Donkey Anti-Rabbit IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20047 | 0.5 mL | |
| CF™647 Donkey Anti-Goat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20048-1 | 50 µL | |
| CF™647 Donkey Anti-Goat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20048 | 0.5 mL | |
| CF™647 Goat Anti-Human IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20280-1 | 50 µL | |
| CF™647 Goat Anti-Human IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20280 | 0.5 mL | |
| CF™647 Goat Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20281-1 | 50 µL | |
| CF™647 Goat Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20281 | 0.5 mL | |
| CF™647 Goat Anti-Rabbit IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20282-1 | 50 µL | |
| CF™647 Goat Anti-Rabbit IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20282 | 0.5 mL | |
| CF™647 Goat Anti-Rat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20283-1 | 50 µL | |
| CF™647 Goat Anti-Rat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20283 | 0.5 mL | |
| CF™647 Donkey Anti-sheep IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20284-1 | 50 µL | |
| CF™647 Donkey Anti-sheep IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20284 | 0.5 mL | |
| CF™647 Rabbit Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20285-1 | 50 µL | |
| CF™647 Rabbit Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20285 | 0.5 mL | |
| CF™647 Goat Anti-Mouse IgG(H+L), 2 mg/mL | 20040-1 | 50 µL | |
| CF™647 Goat Anti-Mouse IgG(H+L), 2 mg/mL | 20040 | 0.5 mL | |
| CF™647 Goat Anti-Guinea Pig IgG(H+L), 2 mg/mL | 20041-1 | 50 µL | |
| CF™647 Goat Anti-Guinea Pig IgG(H+L), 2 mg/mL | 20041 | 0.5 mL | |
| CF™647 Goat Anti-Rabbit IgG(H+L), 2 mg/mL | 20043-1 | 50 µL | |
| CF™647 Goat Anti-Rabbit IgG(H+L), 2 mg/mL | 20043 | 0.5 mL | |
| CF™647 Rabbit Anti-Goat IgG(H+L), 2 mg/mL | 20049-1 | 50 µL | |
| CF™647 Rabbit Anti-Goat IgG(H+L), 2 mg/mL | 20049 | 0.5 mL | |
| CF™647 Goat Anti-Mouse IgG1(γ1), 2 mg/mL | 20252-1 | 50 µL | |
| CF™647 Goat Anti-Mouse IgG1(γ1), 2 mg/mL | 20252 | 0.25 mL | |
| CF™647 Goat Anti-Mouse IgG2a(γ2a), 2 mg/mL | 20262-1 | 50 µL | |
| CF™647 Goat Anti-Mouse IgG2a(γ2a), 2 mg/mL | 20262 | 0.25 mL | |
| CF™647 Goat Anti-Mouse IgG2b(γ2b), 2 mg/mL | 20272-1 | 50 µL | |
| CF™647 Goat Anti-Mouse IgG2b(γ2b), 2 mg/mL | 20272 | 0.25 mL | |
| CF™647 Goat Anti-Swine IgG(H+L), 2 mg/mL | 20286-1 | 50 µL | |
| CF™647 Goat Anti-Swine IgG(H+L), 2 mg/mL | 20286 | 0.5 mL | |
| CF™647 Goat Anti-Human IgM(mu chain), 2 mg/mL | 20346-50uL | 50 µL | |
| CF™647 Goat Anti-Human IgM(mu chain), 2 mg/mL | 20346-250uL | 250 µL | |
| CF™647 F(ab')2 Fragment of Goat Anti-Mouse IgG(H+L), 2 mg/mL | 20042-1 | 50 µL | |
| CF™647 F(ab')2 Fragment of Goat Anti-Mouse IgG(H+L), 2 mg/mL | 20042 | 0.25 mL | |
| CF™647 F(ab')2 Fragment of Goat Anti-Rabbit IgG(H+L), 2 mg/mL | 20045 | 0.25 mL | |
| Annexin V, CF™647 Conjugate | 29003 | 0.5 mL | |
| Streptavidin, CF™647 Conjugate | 29039 | 1 mg | |
| Phalloidin, CF™647 | 00041-T | 50 U | |
| Phalloidin, CF™647 | 00041 | 300 U | |
| CF™647 Azide | 92084 | 0.5 mg | |
| CF™647 Amine | 92042 | 1 mg | |
| CF™647 Alkyne | 92090 | 0.5 mg | |
| CF™647, Succinimidyl Ester | 92135 | 1 µmol | |
| CF™647 Hydrazide | 92136 | 1 mg | |
| CF™647 Maleimide | 92027 | 1 µmol | |
| Mix-n-Stain™ CF™647 Antibody Labeling Kit, 1×(5-20 µg) labeling | 92279 | 1 kit | |
| Mix-n-Stain™ CF™647 Antibody Labeling Kit, 1×(20-50 µg) labeling | 92259 | 1 kit | |
| Mix-n-Stain™ CF™647 Antibody Labeling Kit, 1×(50-100 µg) labeling | 92238 | 1 kit | |
| CF™647 Protein Labeling Kit 3 labelings | 92218 | 1 kit | |
CF™ 660C
製品説明
CF™ 660C 標識二次抗体の励起/蛍光スペクトル
Technical Summary
極大励起波長 / 極大蛍光波長：667 / 685 nm
分子吸光係数：200,000
分子量：〜3112
フローサイトメトリーレーザーライン：633 or 635 nm
顕微鏡レーザーライン：633 or 635 nm
CF™ 660C 標識二次抗体の蛍光強度の比較
CF™ 660C、CF™ 660R および A 社蛍光色素標識の Goat Anti-Mouse IgG を用いて、相対的な蛍光強度を測定しました。蛍光は、633 nm の励起光を用いて、各色素の極大蛍光波長で測定しました。CF™ 660C 標識 Goat Anti-Mouse IgG は、A 社製品と比較し、蛍光強度の強いことが示されています。
DOL：蛍光標識の程度
CF™ 660C 標識二次抗体の退色性の比較
HeLa 細胞を、一次抗体に α-チューブリン抗体（マウス）、二次抗体に CF™ 660C、CF™ 660R および A 社蛍光色素標識の Goat Anti-Mouse IgG を用いて染色しました。データはオリンパス社の水銀ランプを光源とする蛍光顕微鏡により、Cy5 フィルター、CCD カメラを用いて検出しました。10 秒間隔で 5 分間蛍光強度測定した結果、CF™ 660R 標識二次抗体は、A 社製品と比較し、退色し難いことが示されています。
価格表
| 製品名 | メーカー 製品番号 | 容量 | オンライン カタログへ |
|---|---|---|---|
| CF™660C Donkey Anti-Goat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20051-1 | 50 µL |  |
| CF™660C Donkey Anti-Goat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20051 | 0.5 mL | |
| CF™660C Goat Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20052-1 | 50 µL | |
| CF™660C Goat Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20052 | 0.5 mL | |
| CF™660C Goat Anti-Mouse IgG(H+L), Highly Cross-adsorbed(min X Rat), 2 mg/mL | 20368-50uL | 50 µL | |
| CF™660C Goat Anti-Mouse IgG(H+L), Highly Cross-adsorbed(min X Rat), 2 mg/mL | 20368-500uL | 0.5 mL | |
| CF™660C Goat Anti-Rabbit IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20369-50uL | 50 µL | |
| CF™660C Goat Anti-Rabbit IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20369-500uL | 0.5 mL | |
| CF™660C Goat Anti-Rat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20370-50uL | 50 µL | |
| CF™660C Goat Anti-Rat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20370-500uL | 0.5 mL | |
| CF™660C Goat Anti-Chicken IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20371-50uL | 50 µL | |
| CF™660C Goat Anti-Chicken IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20371-500uL | 0.5 mL | |
| CF™660C Donkey Anti-Guinea Pig IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20372-50uL | 50 µL | |
| CF™660C Donkey Anti-Guinea Pig IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20372-500uL | 0.5 mL | |
| CF™660C Goat Anti-Mouse IgG(H+L), 2 mg/mL | 20050-1 | 50 µL | |
| CF™660C Goat Anti-Mouse IgG(H+L), 2 mg/mL | 20050 | 0.5 mL | |
| CF™660C Goat Anti-Rabbit IgG(H+L), 2 mg/mL | 20053-1 | 50 µL | |
| CF™660C Goat Anti-Rabbit IgG(H+L), 2 mg/mL | 20053 | 0.5 mL | |
| Phalloidin, CF™660C | 00052-T | 50 U | |
| Phalloidin, CF™660C | 00052 | 300 U | |
| CF™660C Azide | 92094 | 0.5 mg | |
| CF™660C Alkyne | 92095 | 0.5 mg | |
| CF™660C, Succinimidyl Ester | 92137 | 1 µmol | |
| CF™660C Maleimide | 92028 | 1 µmol | |
| Mix-n-Stain™ CF™660C Antibody Labeling Kit, 1×(5-20 µg) labeling | 92280 | 1 kit | |
| Mix-n-Stain™ CF™660C Antibody Labeling Kit, 1×(20-50 µg) labeling | 92260 | 1 kit | |
| Mix-n-Stain™ CF™660C Antibody Labeling Kit, 1×(50-100 µg) labeling | 92239 | 1 kit | |
| CF™660C Protein Labeling Kit 3 labelings | 92219 | 1 kit | |
CF™ 660R
製品説明
CF™ 660R 標識二次抗体の励起/蛍光スペクトル
Technical Summary
極大励起波長 / 極大蛍光波長：663 / 682 nm
分子吸光係数：100,000
分子量：〜888
フローサイトメトリーレーザーライン：633 or 635 nm
顕微鏡レーザーライン：633 or 635 nm
CF™ 660R 標識二次抗体の蛍光強度の比較
CF™ 660C、CF™ 660R および A 社蛍光色素標識の Goat Anti-Mouse IgG を用いて、相対的な蛍光強度を測定しました。蛍光は、633 nm の励起光を用いて、各色素の極大蛍光波長で測定しました。CF™ 660C 標識 Goat Anti-Mouse IgG は、A 社製品と比較し、蛍光強度の強いことが示されています。
DOL：蛍光標識の程度
CF™ 660R 標識二次抗体の退色性の比較
HeLa 細胞を、一次抗体に α-チューブリン抗体（マウス）、二次抗体に CF™ 660C、CF™ 660R および A 社蛍光色素標識の Goat Anti-Mouse IgG を用いて染色しました。データはオリンパス社の水銀ランプを光源とする蛍光顕微鏡により、Cy5 フィルター、CCD カメラを用いて検出しました。10 秒間隔で 5 分間蛍光強度測定した結果、CF™ 660R 標識二次抗体は、A 社製品と比較し、退色し難いことが示されています。
価格表
| 製品名 | メーカー 製品番号 | 容量 | オンライン カタログへ |
|---|---|---|---|
| CF™660R Goat Anti-Mouse IgG(H+L), 2 mg/mL | 20054-1 | 50 µL | |
| CF™660R Goat Anti-Mouse IgG(H+L), 2 mg/mL | 20054 | 0.5 mL | |
| CF™660R Goat Anti-Rabbit IgG(H+L), 2 mg/mL | 20055-1 | 50 µL | |
| CF™660R Goat Anti-Rabbit IgG(H+L), 2 mg/mL | 20055 | 0.5 mL | |
| Streptavidin, CF™660R Conjugate | 29040 | 1 mg | |
| Phalloidin, CF™660R | 00047-T | 50 U | |
| Phalloidin, CF™660R | 00047 | 300 U | |
| CF™660R Aminooxy | 92059 | 1 mg | |
| CF™660R Succinimidyl Ester | 92134 | 1 µmol | |
| CF™660R Maleimide | 92031 | 1 µmol | |
| Mix-n-Stain™ CF™660R Antibody Labeling Kit, 1×(5-20 µg) labeling | 92281 | 1 kit | |
| Mix-n-Stain™ CF™660R Antibody Labeling Kit, 1×(20-50 µg) labeling | 92261 | 1 kit | |
| Mix-n-Stain™ CF™660R Antibody Labeling Kit, 1×(50-100 µg) labeling | 92243 | 1 kit | |
| CF™660R Protein Labeling Kit 3 labelings | 92223 | 1 kit | |
CF™ 680
製品説明
CF™ 680 標識二次抗体の励起/蛍光スペクトル
CF™ 680 標識二次抗体の蛍光強度の比較
HeLa 細胞のライセートを 2 倍ずつ希釈し、アクリルアミドゲルで電気泳動後、ニトロセルロース膜に転写し、一次抗体に抗 α-チューブリン抗体（マウス）と抗 COX IV 抗体（ラビット）、二次抗体に以下の蛍光色素標識抗体を同一濃度で使用し染色しました。
CF™ 770 標識 Goat Anti-Mouse IgG
F 社蛍光色素標識 Goat Anti-Mouse IgG
CF™ 680 標識 Goat Anti-Rabbit IgG
F 社蛍光色素標識 Goat Anti-Rabbit IgG
膜を乾燥後、蛍光検出は LI-COR Biosciences 社の Odyssey® Infrared Imaging System により測定しました。その結果、CF™ 色素標識二次抗体は F 社製品と比較し、約 3.5 倍以上の蛍光強度が得られることが示されています。
使用例
CF™ 680 標識二次抗体を用いた染色
HeLa 細胞を、一次抗体に抗 α-チューブリン抗体（マウス）、二次抗体に CF™ 680 標識 Goat Anti-Mouse IgG を用いて染色しました。データはオリンパス社の水銀ランプを光源とする顕微鏡により、Cy5 フィルター、CCD カメラ、Image-Pro Express software を用いて検出しました。
価格表
| 製品名 | メーカー 製品番号 | 容量 | オンライン カタログへ |
|---|---|---|---|
| CF™680 Donkey Anti-Goat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20060-1 | 50 µL | |
| CF™680 Donkey Anti-Goat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20060 | 0.25 mL | |
| CF™680 Rabbit Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20061-1 | 50 µL | |
| CF™680 Rabbit Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20061 | 0.25 mL | |
| CF™680 Donkey Anti-sheep IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20062-1 | 50 µL | |
| CF™680 Donkey Anti-sheep IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20062 | 0.25 mL | |
| CF™680 Goat Anti-Mouse IgG(H+L), highly cross-absorbed, 2 mg/mL | 20065-1 | 50 µL | |
| CF™680 Goat Anti-Mouse IgG(H+L), highly cross-absorbed, 2 mg/mL | 20065 | 0.25 mL | |
| CF™680 Goat Anti-Rabbit IgG(H+L), highly cross-absorbed, 2 mg/mL | 20067-1 | 50 µL | |
| CF™680 Goat Anti-Rabbit IgG(H+L), highly cross-absorbed, 2 mg/mL | 20067 | 0.25 mL | |
| CF™680 Goat Anti-Rat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20069-1 | 50 µL | |
| CF™680 Goat Anti-Rat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20069 | 0.25 mL | |
| CF™680 Donkey Anti-Guinea Pig IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20241-1 | 50 µL | |
| CF™680 Donkey Anti-Guinea Pig IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20241 | 0.25 mL | |
| CF™680 Donkey Anti-Human IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20278-1 | 50 µL | |
| CF™680 Donkey Anti-Human IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20278 | 0.25 mL | |
| CF™680 Goat Anti-Human IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20287-1 | 50 µL | |
| CF™680 Goat Anti-Human IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20287 | 0.25 mL | |
| CF™680 Rabbit Anti-Goat IgG(H+L), 2 mg/mL | 20068-1 | 50 µL | |
| CF™680 Rabbit Anti-Goat IgG(H+L), 2 mg/mL | 20068 | 0.5 mL | |
| CF™680 Rabbit Anti-guiea pig IgG(H+L), 2 mg/mL | 20243-1 | 50 µL | |
| CF™680 Rabbit Anti-guiea pig IgG(H+L), 2 mg/mL | 20243 | 0.5 mL | |
| CF™680 Goat Anti-Mouse IgG1(γ1), 2 mg/mL | 20253-1 | 50 µL | |
| CF™680 Goat Anti-Mouse IgG1(γ1), 2 mg/mL | 20253 | 0.25 mL | |
| CF™680 Goat Anti-Mouse IgG2a(γ2a), 2 mg/mL | 20263-1 | 50 µL | |
| CF™680 Goat Anti-Mouse IgG2a(γ2a), 2 mg/mL | 20263 | 0.25 mL | |
| CF™680 Goat Anti-Mouse IgG2b(γ2b), 2 mg/mL | 20273-1 | 50 µL | |
| CF™680 Goat Anti-Mouse IgG2b(γ2b), 2 mg/mL | 20273 | 0.25 mL | |
| CF™680 Goat Anti-Mouse IgM(mu chain), 2 mg/mL | 20384-50uL | 50 µL | |
| CF™680 Goat Anti-Mouse IgM(mu chain), 2 mg/mL | 20384-250uL | 250 µL | |
| CF™680 F(ab')2 Fragment of Goat Anti-Mouse IgG(H+L), 2 mg/mL | 20063 | 0.25 mL | |
| CF™680 F(ab')2 Fragment of Goat Anti-Rabbit IgG(H+L), 2 mg/mL | 20064 | 0.25 mL | |
| CF™680 Monoclonal Mouse Anti-6X His IgG, 1mg/ml | 20359 | 50 µL | |
| CF™680 Monoclonal Mouse Anti-GFP IgG, 1mg/ml | 20219 | 0.1 mL | |
| Annexin V, CF™680 Conjugate | 29007 | 25 µg | |
| CF™680, Succinimidyl Ester | 92139 | 1 µmol | |
| CF™680 Maleimide | 92029 | 1 µmol | |
| Mix-n-Stain™ CF™680 Antibody Labeling Kit, 1×(5-20 µg) labeling | 92282 | 1 kit | |
| Mix-n-Stain™ CF™680 Antibody Labeling Kit, 1×(20-50 µg) labeling | 92262 | 1 kit | |
| Mix-n-Stain™ CF™680 Antibody Labeling Kit, 1×(50-100 µg) labeling | 92240 | 1 kit | |
| VivoBrite™ Rapid Antibody Labeling Kit for Small Animal In Vivo Imaging, CF™680 SE | 92160 | 1 kit | |
| CF™680 Protein Labeling Kit 3 labelings | 92220 | 1 kit | |
CF™ 680R
製品説明
CF™ 680R 標識二次抗体の励起/蛍光スペクトル
価格表
| 製品名 | メーカー 製品番号 | 容量 | オンライン カタログへ |
|---|---|---|---|
| CF™680R Goat Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20192-1 | 50 µL | |
| CF™680R Goat Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20192 | 0.25 mL | |
| CF™680R Goat Anti-Rabbit IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20193-1 | 50 µL | |
| CF™680R Goat Anti-Rabbit IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20193 | 0.25 mL | |
| CF™680R Donkey Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20194-1 | 50 µL | |
| CF™680R Donkey Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20194 | 0.25 mL | |
| CF™680R Donkey Anti-Rabbit IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20195-1 | 50 µL | |
| CF™680R Donkey Anti-Rabbit IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20195 | 0.25 mL | |
| CF™680R Donkey Anti-Goat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20196-1 | 50 µL | |
| CF™680R Donkey Anti-Goat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20196 | 0.25 mL | |
| Wheat Germ Agglutinin, CF™680R Conjugate | 29025-1 | 1 mg | |
| Wheat Germ Agglutinin, CF™680R Conjugate | 29025 | 5×1 mg | |
| Human Transferrin, CF™680R Conjugate | 00086 | 1 mg | |
| Phalloidin, CF™680R | 00048-T | 50 U | |
| Phalloidin, CF™680R | 00048 | 300 U | |
| α-Bungarotoxin, CF™680R | 00003 | 0.5 mg | |
| CF™680R-dUTP, lyophilized powder | 40003 | 25 nmol | |
| CF™680R, Aminooxy | 92054 | 1 mg | |
| CF™680R, Succinimidyl Ester | 92107 | 1 µmol | |
| CF™680R Maleimide | 92032 | 1 µmol | |
| Mix-n-Stain™ CF™680R Antibody Labeling Kit, 1×(5-20 µg) labeling | 92283 | 1 kit | |
| Mix-n-Stain™ CF™680R Antibody Labeling Kit, 1×(20-50 µg) labeling | 92263 | 1 kit | |
| Mix-n-Stain™ CF™680R Antibody Labeling Kit, 1×(50-100 µg) labeling | 92246 | 1 kit | |
| CF™680R Protein Labeling Kit 3 labelings | 92226 | 1 kit | |
CF™ 750
製品説明
CF™ 750 標識二次抗体の励起/蛍光スペクトル
CF™ 750 の安定性の比較
CF™ 750 および各社蛍光色素に太陽光を 30 分間照射しました。スペクトルは、照射前後の励起スペクトルを示しています。CF™ 750 は照射後にも色素が安定であることが示されています。
黒：照射前、灰：照射後
CF™ 750 標識二次抗体の蛍光強度の比較
CF™ 750 および A 社、B 社の蛍光色素標識の Goat Anti-Mouse IgG の蛍光を日立分光蛍光光度計 F-4500 により測定しました。その結果、CF™ 750 標識二次抗体は、A 社および B 社蛍光色素標識二次抗体と比較し、高い蛍光強度を持つことが示されています。
DOL：蛍光標識の程度
CF™ 750 標識二次抗体の S/N 比の比較
Jurkat 細胞を、一次抗体に CD3 に対する抗体と Isotype、二次抗体に CF™ 750 および A 社蛍光色素標識の Goat Anti-Mouse IgG を用い、BD LSR II フローサイトメーター（633 nmレーザー/780/60 nm PMT 検出器搭載）により蛍光強度を測定し、S/N 比を算出しました。その結果、CF™ 750 標識二次抗体は高い S/N 比を得ることが示されています。
使用例
CF™ 750 標識体を用いた in vivo イメージング
CF™ 750 で標識したアバスチン®をマウスに静脈注射し、24 時間後と 96 時間後に IVIS イメージングシステムを用いて検出しました。腫瘍部位に時間経過とともに高濃度で集まってきていることが体外から測定できました。このことより、近赤外色素 CF™ 750 が in vivo イメージングに適することが示されています。
価格表
| 製品名 | メーカー 製品番号 | 容量 | オンライン カタログへ |
|---|---|---|---|
| CF™ 750 Donkey Anti-Rabbit IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20298-1 | 50 µL | |
| CF™ 750 Donkey Anti-Rabbit IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20298 | 0.25 mL | |
| CF™ 750 Donkey Anti-Goat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20362-50uL | 50 µL | |
| CF™ 750 Donkey Anti-Goat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20362-250uL | 0.25 mL | |
| CF™ 750 Goat Anti-Mouse IgG(H+L), 2 mg/mL | 20070-1 | 50 µL | |
| CF™ 750 Goat Anti-Mouse IgG(H+L), 2 mg/mL | 20070 | 0.5 mL | |
| CF™ 750 Goat Anti-Rabbit IgG(H+L), 2 mg/mL | 20073-1 | 50 µL | |
| CF™ 750 Goat Anti-Rabbit IgG(H+L), 2 mg/mL | 20073 | 0.5 mL | |
| Annexin V, CF™ 750 Conjugate | 29006 | 25 µg | |
| CF™ 750, Succinimidyl Ester | 92142 | 1 µmol | |
| Mix-n-Stain™ CF™ 750 Antibody Labeling Kit, 1×(5-20 µg) labeling | 92284 | 1 kit | |
| Mix-n-Stain™ CF™ 750 Antibody Labeling Kit, 1×(20-50 µg) labeling | 92264 | 1 kit | |
| Mix-n-Stain™ CF™ 750 Antibody Labeling Kit, 1×(50-100 µg) labeling | 92241 | 1 kit | |
| VivoBrite™ Rapid Antibody Labeling Kit for Small Animal In Vivo Imaging, CF™ 750 SE | 92161 | 1 kit | |
| CF™ 750 Protein Labeling Kit 3 labelings | 92221 | 1 kit | |
CF™ 770
製品説明
CF™ 770 標識二次抗体の励起/蛍光スペクトル
CF™ 770 標識二次抗体の蛍光強度の比較
HeLa 細胞のライセートを 2 倍ずつ希釈し、アクリルアミドゲルで電気泳動後、ニトロセルロース膜に転写し、一次抗体に抗 α-チューブリン抗体（マウス）と抗 COX IV 抗体（ラビット）、二次抗体に以下の蛍光色素標識抗体を同一濃度で使用し染色しました。
CF™ 770 標識 Goat Anti-Mouse IgG
F 社蛍光色素標識 Goat Anti-Mouse IgG
CF™ 680 標識 Goat Anti-Rabbit IgG
F 社蛍光色素標識 Goat Anti-Rabbit IgG
膜を乾燥後、蛍光検出は LI-COR Biosciences 社の Odyssey® Infrared Imaging System により測定しました。その結果、CF™ 色素標識二次抗体は F 社製品と比較し、約 3.5 倍以上の蛍光強度が得られることが示されています。
価格表
| 製品名 | メーカー 製品番号 | 容量 | オンライン カタログへ |
|---|---|---|---|
| CF™ 770 Goat Anti-Mouse IgG(H+L), highly cross-absorbed, 2 mg/mL | 20077-1 | 50 µL | |
| CF™ 770 Goat Anti-Mouse IgG(H+L), highly cross-absorbed, 2 mg/mL | 20077 | 0.25 mL | |
| CF™ 770 Goat Anti-Rabbit IgG(H+L), highly cross-absorbed, 2 mg/mL | 20078-1 | 50 µL | |
| CF™ 770 Goat Anti-Rabbit IgG(H+L), highly cross-absorbed, 2 mg/mL | 20078 | 0.25 mL | |
| CF™ 770 Donkey Anti-Guinea Pig IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20242-1 | 50 µL | |
| CF™ 770 Donkey Anti-Guinea Pig IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20242 | 0.25 mL | |
| CF™ 770 Donkey Anti-Goat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20277-1 | 50 µL | |
| CF™ 770 Donkey Anti-Goat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20277 | 0.25 mL | |
| CF™ 770 Donkey Anti-Human IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20279-1 | 50 µL | |
| CF™ 770 Donkey Anti-Human IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20279 | 0.25 mL | |
| CF™ 770 Goat Anti-Human IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20288-1 | 50 µL | |
| CF™ 770 Goat Anti-Human IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20288 | 0.25 mL | |
| CF™ 770 Goat Anti-Rat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20383-50uL | 50 µL | |
| CF™ 770 Goat Anti-Rat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20383-250uL | 0.25 mL | |
| CF™ 770 Rabbit Anti-guiea pig IgG(H+L), 2 mg/mL | 20244-1 | 50 µL | |
| CF™ 770 Rabbit Anti-guiea pig IgG(H+L), 2 mg/mL | 20244 | 0.5 mL | |
| CF™ 770 Goat Anti-Mouse IgG1(γ1), 2 mg/mL | 20254-1 | 50 µL | |
| CF™ 770 Goat Anti-Mouse IgG1(γ1), 2 mg/mL | 20254 | 0.25 mL | |
| CF™ 770 Goat Anti-Mouse IgG2a(γ2a), 2 mg/mL | 20264-1 | 50 µL | |
| CF™ 770 Goat Anti-Mouse IgG2a(γ2a), 2 mg/mL | 20264 | 0.25 mL | |
| CF™ 770 Goat Anti-Mouse IgG2b(γ2b), 2 mg/mL | 20274-1 | 50 µL | |
| CF™ 770 Goat Anti-Mouse IgG2b(γ2b), 2 mg/mL | 20274 | 0.25 mL | |
| CF™ 770 Goat Anti-Mouse IgM(mu chain), 2 mg/mL | 20385-50uL | 50 µL | |
| CF™ 770 Goat Anti-Mouse IgM(mu chain), 2 mg/mL | 20385-250uL | 250 µL | |
| CF™ 770 Monoclonal Mouse Anti-GFP IgG, 1mg/ml | 20220 | 0.1 mL | |
| CF™ 770 Monoclonal Mouse Anti-6X His IgG, 1mg/ml | 20360 | 50 µL | |
| CF™ 770 Monoclonal Mouse Anti-Biotin IgG, 2 mg/mL | 20367-50uL | 50 µL | |
| CF™ 770 Monoclonal Mouse Anti-Biotin IgG, 2 mg/mL | 20367-250uL | 250 µL | |
| Annexin V, CF™ 770 | 29046 | 25 µg | |
| CF™ 770 Amine | 92065 | 1 mg | |
| CF™ 770, Succinimidyl Ester | 92150 | 1 µmol | |
| Mix-n-Stain™ CF™ 770 Antibody Labeling Kit, 1×(5-20 µg) labeling | 92285 | 1 kit | |
| Mix-n-Stain™ CF™ 770 Antibody Labeling Kit, 1×(20-50 µg) labeling | 92265 | 1 kit | |
| Mix-n-Stain™ CF™ 770 Antibody Labeling Kit, 1×(50-100 µg) labeling | 92242 | 1 kit | |
| VivoBrite™ Rapid Antibody Labeling Kit for Small Animal In Vivo Imaging, CF™ 770 SE | 92162 | 1 kit | |
| CF™ 770 Protein Labeling Kit 3 labelings | 92222 | 1 kit | |
CF™ 790
価格表
| 製品名 | メーカー 製品番号 | 容量 | オンライン カタログへ |
|---|---|---|---|
| CF™ 790 Goat Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20342-50uL | 50 µL | |
| CF™ 790 Goat Anti-Rabbit IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20343-50uL | 50 µL | |
| CF™ 790 Donkey Anti-Rabbit IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20344-50uL | 50 µL | |
| CF™ 790 Donkey Anti-Goat IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20345-50uL | 50 µL | |
| CF™ 790 Donkey Anti-Mouse IgG(H+L), Highly Cross-adsorbed, 2 mg/mL | 20363-50uL | 50 µL | |
| CF™ 790 Goat Anti-Mouse IgG(H+L), 2 mg/mL | 20378-50uL | 50 µL | |
| CF™ 790 Goat Anti-Rabbit IgG(H+L), 2 mg/mL | 20379-50uL | 50 µL | |
| Annexin V, CF™ 790 | 29047 | 25 µg | |
| CF™ 790, Succinimidyl Ester | 92155 | 0.25 µmol | |
| Mix-n-Stain™ CF™ 790 Antibody Labeling Kit, 1×(5-20 µg) labeling | 92288 | 1 kit | |
| Mix-n-Stain™ CF™ 790 Antibody Labeling Kit, 1×(20-50 µg) labeling | 92268 | 1 kit | |
| Mix-n-Stain™ CF™ 790 Antibody Labeling Kit, 1×(50-100 µg) labeling | 92248 | 1 kit | |
| VivoBrite™ Rapid Abtibody Labeling Kit for Small Animal In Vivo Imaging, CF™ 790 SE | 92163 | 1 kit | |
※記載の内容は、'16年11月現在の情報に基づいております。
※ご所属などの情報は、データご提供時の情報に基づいています。
