Jena Bioscience社 タンパク質蛍光ラベリングキット
C末端、N末端、または両端を特異的に蛍光標識できます！
The novel labeling kits presented here are designed for site-specific labeling of
- any protein’s C-terminus using convenient, fast, mild and biocompatible oxyamine–ketone ligation (scheme),
- an N-terminal cysteine using native chemical ligation with a fluorophore-thioester, or
- a protein’s C & N-termini simultaneously with two colors in a One-Pot Dual Color labeling reaction for fluorescence resonance energy transfer (FRET) studies
General method for protein modification at the C terminus: Under mild and biocompatible conditions a protein thioester is transformed with 1,2-bis(oxyamino)ethane into an oxyamino-modified species that in a second step reacts rapidly with ketones carrying a fluorescent label.
(adapted from Yi et al. (2010))
| Product | Cat. No. | Amount | Downloads |
|---|---|---|---|
| C-terminal Protein Labeling Kit Universal C-terminal Protein Labeling with Coumarin |
FP-301-COU | 1 Kit (for labeling of 5 mg protein) |
 |
| N-terminal Protein Labeling Kit Universal N-terminal Protein Labeling with Coumarin |
FP-302-COU | 1 Kit (for labeling of 5 mg protein) |
|
| Protein Dual-Labeling Kit Universal C/N-terminal Protein Labeling with Fluorescein/Coumarin |
FP-303-FLU | 1 Kit (for labeling of 5 mg protein) |
|
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