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AAT Bioquest社　リソソーム染色キット

明るく退色しにくい色素

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Lysosomes are cellular organelles which contain acid hydrolase enzymes to break up waste materials and cellular debris. Lysosomes
digest excess or worn-out organelles, food particles, and engulfed viruses or bacteria. The membrane around a lysosome allows the digestive enzymes to work at pH 4.5. The interior of the lysosome is acidic (pH 4.5-4.8) compared to the slightly alkaline
cytosol (pH 7.2). The lysosome maintains this pH differential by pumping protons from the cytosol across the membrane via proton pumps and chloride ion channels.

AAT Bioquest Cell Navigator™ fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as membranes, lysosomes, mitochondria, nuclei, etc. The selective labeling of live cell compartments provides a powerful method for studying cellular events in a spatial and temporal context.
Our Cell Navigator™ Lysosome Staining Kits are designed to label lysosomes of live cells with LysoBrite™ dyes, our proprietary lysotropic indicators which selectively accumulate in lysosomes probably via the lysosome pH gradient. The lysoBrite™ dyes are hydrophobic compounds that easily permeate intact live cells, and trapped in lysosomes after they get into cells. Their fluorescence

is significantly enhanced upon entering lysosomes. This key feature significantly reduces the staining background and makes the assay kits useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis
and cytotoxicity. The Cell Navigator™ staining kits are suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells. The labeling protocols are robust, requiring minimal hands-on time. The kits can be readily adapted for many types of fluorescence platforms such as microplate
assays, flow cytometry and fluorescence microscope.

Key Features of Cell Navigator™ Staining Kits:
• Minimal cytotoxicity, no cell toxicity observed.
• Multiplexing wavelengths, Ex/Em = 450/505 nm, 542/556 nm, 575/597 nm and 596/619 nm.
• Increased signal intensity, 10 times brighter than the product of Company A.
• Extraordinarily high photostability, no fading observed with 2 minutes exposure.
• Excellent cellular retention, more than 6 passages for cell tracking in Hela cells.
• Fixable, cell staining pattern survives fixation.

 

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Figure 1. Images of Hela cells stained with A: Cell Navigator™ Lysosome Staining Kit, B: the product from Company A in a Costar blackwall/clear bottom 96-well plate. The signals were compared at 0 and 120 seconds exposure time by using an Olympus fluorescence microscope.

 

 

lysosome fig2.JPG

Figure 2. Images of Hela cells stained with A: Cell

Navigator Lysosome Staining Kit (Top, from AAT Bioquest), B: the product from Company A in a Costar black wall/clear bottom 96-well plate. The signals were compared at 5 cell passages (P1, P2, P3, P4 and P5) respectively using an Olympus fluorescence microscope.

Cat.# Product Description Ex(nm) Em(nm) Size
22656 Cell Navigator Lysosome Staining Kit Green Fluorescence 450 505 500assay
22657 Cell Navigator Lysosome Staining Kit Orange Fluorescence 542 556 500assay
22658 Cell Navigator Lysosome Staining Kit Red Fluorescence 575 597 500assay
22659 Cell Navigator Lysosome Staining Kit Deep Red Fluorescence 596 619 500assay

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