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AAT Bioquest社 Calcium Visualization For All Platforms


Modern Calcium Imaging

Calcium is a ubiquitous cellular messenger impacting a number of signal transduction pathways, with multiple roles in processes ranging from neurotransmission to muscle contraction. Single-channel indicators, ratiometric probes, and calcium flux assays to track calcium mobilization are all in perpetually high demand.

We are proud to have developed the largest collection of calcium indicators, including well-known favorites Fluo-8® AM, Cal-520® AM, and Calbryte™ 520 AM. We have also expanded our offerings of calcium flux assays and indicator conjugates, with intense fluorescent signals and in a variety of wavelength options ranging from ultraviolet to infrared.


ATP response measured in CHO-K1 cells using either Calbryte™ 520 AM or 10 µg/ml Fluo-4, AM. Imaged with microscope FITC channel (Keyence).

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Useful Resources &

Featured Products

Many resources and specialized products for researchers are available, from Application Notes to FAQs, as well as tools and protocols.

In-House Research Publication
AssayWise Letters Open-Access Article:
Prolonged Retention of Calbryte™ 520 AM for In Vitro Analysis

Webinar Recording
Open-Access Calcium Imaging Webinar
A Series of New and Improved Calcium Indicators for Functional
Analysis of GPCRs and Calcium Channel Targets

G-Protein-Coupled Receptors (GPCR):
ScreenQuest GPCR Receptor Cells & Chimera Cell Lines

Cell Signaling Assays:
ADP, ATP, & cAMP Assays

Classic Calcium Indicators:
Fluo-3 AM
Fluo-4 AM


Featured Citation In Vivo Ca2+ dynamics and muscle displacement of the beetle leg muscle under electrical stimulation with multiple pulse trains. Pseudocolor time series images of beetle leg muscle dosed with indicators Fluo-8 (top) and Cell Tracker (bottom). From: Oral Dosing of Chemical Indicators for In Vivo Monitoring of Ca2+ Dynamics in Insect Muscle by Ferdinandus et al., PLOS, Jan. 2015.

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