Cell Transfection:

Lipofectamine™ vs. Transfectamine™

Physical transfection methods such as electroporation or microinjection tend to have low efficiency and damage cells.

Chemical-based transfection uses materials ranging from inexpensive calcium phosphate and organic dendrimers to advanced ones like Lipofectamine™ and Transfectamine™, but which must be optimized.

There are 2 key issues with transfection:
• Chronically low efficiency
• Cytotoxicity associated with the transfection procedure.

Through rigorous in-house testing, we have successfully shown that cells transfected with Transfectamine™ 5000 provides much higher transfection efficiency with low cytotoxicity, as compared with similar reagents. 

Comparison of Transfection Efficiency: CHO-K1 cells were cultured to ~90% confluency. GFP plasmid was transfected with Lipofectamine™ 2000, Lipofectamine™ 3000 and Transfectamine™ 5000. Images taken 24 hours post transfection with fluorescent microscope in FITC channel.