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AAT Bioquest社 Cell Transfection Efficiency: Lipofectamine or Transfectamine?



Cell Transfection:

Lipofectamine™ vs. Transfectamine™


Physical transfection methods such as electroporation or microinjection tend to have low efficiency and damage cells.

Chemical-based transfection uses materials ranging from inexpensive calcium phosphate and organic dendrimers to advanced ones like Lipofectamine™ and Transfectamine™, but which must be optimized.

There are 2 key issues with transfection:
• Chronically low efficiency
• Cytotoxicity associated with the transfection procedure.

Through rigorous
in-house testing, we have successfully shown that cells transfected with Transfectamine™ 5000 provides much higher transfection efficiency with low cytotoxicity, as compared with similar reagents. 


Comparison of Transfection Efficiency: CHO-K1 cells were cultured to ~90% confluency. GFP plasmid was transfected with Lipofectamine™ 2000, Lipofectamine™ 3000 and Transfectamine™ 5000. Images taken 24 hours post transfection with fluorescent microscope in FITC channel.

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Featured Products & Resources

AAT Bioquest provides a wide range of resources and specialized products for researchers transfecting cells in the lab, from Application Notes to FAQs.

AssayWise Letters Research Article:
Transfectamine™ 5000: An Efficient and Reliable DNA Delivery System

Drug Discovery Targets:
G-Protein-Coupled Receptors (GPCR)

Open-Access Tools
Online Calculators for DNA Research:
DNA Concentration Calculator
DNA Molecular Weight Calculator

Post_Transfection_Activity_Comparison.pngPost-Transfection Activity Comparison: Activation of GPCR V2R construct transfected CHO-K1 cells with Vasopressin. The promiscuous G-protein Ga16 and GPCR V2R construct were co-transfected by Lipofectamine™ 2000, Lipofectamine™ 3000, or Transfectamine™ 5000. Rapid calcium flux was visualized using the fluorescent calcium dye Calbryte™ 520 AM.

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