この製品に関するご意見・ご照会・お問合せはこちら

Screen & Evaluate ACE2 Inhibitors

Fluorogenic ACE2 Substrate for SARS-CoV-2 Research

Mca-APK(Dnp) ACE2 substrate is a FRET peptide for measuring enzymatic activity in cells and tissues.
This fluorogenic substrate offers more flexibility and higher throughput than the commonly used HPLC-separation-based methods.

Chemical structure for Mca-APK(Dnp) ACE2 substrate. The attached DNP acts as a quencher molecule to quench the fluorescence of methoxycoumarin (Mca). 

The spike protein of SARS-CoV-2 utilizes the ACE2 surface receptor on host cells to promote cellular entry, making it a promising therapeutic target for SARS-CoV-2. The loss of ACE2 function following binding by SARS-CoV-2 is driven by endocytosis and activation of proteolytic cleavage and processing.

To screen and evaluate ACE2 inhibitors AAT Bioquest offers Mca-APK(Dnp), a fluorogenic ACE2 substrate:

• The substrate has an ex/em maxima of 322 nm and 381 nm, respectively
• Uses DNP as a quencher molecule to quench the fluorescence of methoxycoumarin (Mca).—more flexibility and higher throughput than the commonly used HPLC-separation-based methods
• Suitable for either kinetic or end-point reading, depending on protocol. 

The binding of viruses to receptors on the surface of target cells is a critical first step in the infectious viral life cycle. For other areas of coronavirus research, click the button below to consult our catalog. 

Mca-APK(Dnp) ACE2 substrates are easily adaptable for high through-put screening. For questions about recommended protocols or applications, contact us. 

520 Mercury Drive Sunnyvale, CA 94085, USA